1.上海中医药大学龙华临床医学院(上海 200032)
2.上海中医药大学附属第七人民医院(上海 200137)
3.上海中医药大学附属龙华医院(上海 200032)
胡俊威,男,在读博士生,主要从事针灸治疗泌尿生殖系统疾病基础研究
陈跃来,主任医师、教授,博士生导师;E-mail:chenyuelai@163.com
纸质出版日期:2024-07-25,
收稿日期:2023-10-24,
修回日期:2024-01-04,
扫 描 看 全 文
胡俊威,金珠,李旭等.小鼠“次髎”穴的取穴方法研究[J].上海中医药大学学报,2024,38(04):55-61.
HU Junwei,JIN Zhu,LI Xu,et al.Study on acupoint positioning method of "Ciliao (BL32)" in mice[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2024,38(04):55-61.
胡俊威,金珠,李旭等.小鼠“次髎”穴的取穴方法研究[J].上海中医药大学学报,2024,38(04):55-61. DOI: 10.16306/j.1008-861x.2024.04.008.
HU Junwei,JIN Zhu,LI Xu,et al.Study on acupoint positioning method of "Ciliao (BL32)" in mice[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2024,38(04):55-61. DOI: 10.16306/j.1008-861x.2024.04.008.
目的
2
探寻小鼠“次髎”穴的取穴方法,为小鼠次髎穴的取穴提供一种准确可行的方案。
方法
2
①取健康成年C57BL小鼠,通过解剖确定次髎穴坐标点;②取成年C57BL小鼠,随机分为实验组和对照组,各组分别采用坐标定位法和“L6 棘突”为骨性标志的穴位定位方法进行取穴,比较两种取穴方法的准确率;③取成年C57BL小鼠,复制间质性膀胱炎(IC)小鼠模型,使用坐标定位法的方法进行取穴治疗,并验证其功能作用。
结果
2
健康成年小鼠的次髎穴坐标为(±1 mm,21 mm,-5 mm),坐标定位法取穴准确率高于骨性标志定位法,且使用坐标定位法取穴电针治疗可以改善IC小鼠相关症状。
结论
2
坐标定位法取穴较骨性标志定位法准确率更高,且具有次髎穴的功能。
Objective: To investigate the positioning method of "Ciliao (BL32)" acupoint in mice, and provide a precise and feasible approach for Ciliao (BL32) acupoint location in mice.
Methods
2
①Healthy adult C57BL mice were taken, and the coordinate point of Ciliao (BL32) was determined by dissection. ②Adult C57BL mice were selected and randomly divided into the experimental group and control group, and the coordinate positioning method and the acupoint positioning method with "L6 spinous process" as the bony sign were respectively applied for acupiont selection. The accuracy of the two acupoint selection methods were compared. ③Adult C57BL mice were selected and the interstitial cystitis (IC) model of mouse was copied. The coordinate positioning method was applied for treatment and its functional effect was verified.
Results
2
The coordinates of Ciliao (BL32) acupoint of healthy adult mice were (±1 mm, 21 mm, -5 mm), and the accuracy of the coordinate positioning method was higher than that of the bone sign positioning method. Meanwhile the electroacupuncture treatment applying the coordinate positioning method could improve the related symptoms of IC mice.
Conclusion
2
The accuracy of the coordinate positioning method is higher than that of the bone sign positioning method, and has the function of Ciliao (BL32) acupoint.
次髎穴小鼠取穴方法坐标定位法间质性膀胱炎
Ciliao (BL32) acupointmiceacupoint positioning methodcoordinate positioning methodinterstitial cystitis
WU J,CHENG Y,QIN Z,et al. Effects of electroacupuncture on bladder and bowel function in patients with transverse myelitis: a prospective observational study[J]. Acupunct Med, 2018, 36(4): 261-266.
ZHANG W,FANG Y,SHI M,et al. Optimal acupoint and session of acupuncture for patients with chronic prostatitis/chronic pelvic pain syndrome: a meta-analysis[J].Transl Androl Urol, 2021, 10(1): 143-153.
WANG S J,YANG H Y,WANG F,et al. Acupoint Specificity on Colorectal Hypersensitivity Alleviated by Acupuncture and the Correlation with the Brain-Gut Axis[J]. Neurochem Res, 2015, 40(6): 1274-1282.
中国针灸学会.实验动物常用穴位名称与定位第3部分:小鼠(T/CAAM 0003-2020) [S]. 针刺研究, 2021, 46(5): 445-446.
Chinese Society of Acupuncture-Moxibustion. Name and location of commonly used acupoints in laboratory animals - Part 3: Mice (T/CAAM 0003-2020)[S]. Acupuncture Research, 21, 46(5): 445-446.
中国针灸学会.实验动物常用穴位名称与定位第2部分:大鼠(T/CAAM 0002-2020)[S]. 针刺研究, 2021, 46(4): 351-352.
Chinese Society of Acupuncture-Moxibustion. Name and location of commonly used acupoints in laboratory animals Part 2: rats (T/CAAM 0002-2020)[S]. Acupuncture Research, 21, 46(4): 351-352.
刘笑萌,许明,邓石峰,等. 大鼠“次髎”穴的取穴方法研究[J].湖南中医药大学学报, 2019, 39(11): 1373-1376.
LIUX M, XU M, DENG S F, et al. Study onacupoint positioning method ofCiliao(BL32) in rats[J]. Journal of Hunan University of Chinese Medicine, 2019, 39 (11): 1373-1376.
Paxinos G,Franklin K B J.Paxinos and Franklin's the Mouse Brain in Stereotaxic Coordinates[M]. Amsterdam: Elsevier/A P,2013.
鲍星奇,黄轶晨,魏思思,等. 藻蓝蛋白对环磷酰胺诱导的小鼠间质性膀胱炎作用的研究[J].现代泌尿外科杂志, 2019, 24(6): 480-484.
BAO X Q, HUANG Y C, WEI S S, et al. Effects of phycocyanin on the cyclophosphamide-induced IC/BPS in murine models[J]. Journal of Modern Urology, 2019, 24 (6): 480-484.
CHEN Z,LIU L,CHEN Y,et al. Periostin attenuates Cyclophosphamide-induced bladder injury by promoting urothelial stem cell proliferation and macrophage polarization[J].Stem Cells Transl Med, 2022, 11(6): 659-673.
刘建党,冯戟玮,胡俊威,等. 针刺对膀胱过度活动大鼠膀胱尿路上皮Piezo1介导机械刺激的影响 [J]. 时珍国医国药, 2022, 33(10): 2539-2542.
LIU J D, FENG J W, HU J W, et al. Effects of acupuncture on PIEZO1-mediated mechanical stimulation of bladder urothelium in rats with overactive bladder[J]. Lishizhen Medicine and Materia Medica Research, 2022, 33 (10): 2539-2542.
GAUDET A D,FONKEN L K,AYALA M T,et al. Aging and miR-155 in mice influence survival and neuropathic pain after spinal cord injury[J]. Brain Behav Immun, 2021, 97: 365-370.
LU Y,LI J,DONG L,et al. Activation of uroepithelial 5-HT(4)R inhibits mechanosensory activity of murine bladder afferent nerves[J]. Front Physiol, 2022, 13: 990178.
WANG L L,REN Z X,ZHU J Y,et al. Efficacy of electroacupuncture combined with penetrating moxibustion for postpartum stress urinary incontinence[J]. Zhongguo Zhen Jiu,2019, 39(6): 599-603.
徐桂兴,罗廖君,银子涵,等. 基于数据挖掘近10年针灸治疗良性前列腺增生症用穴规律研究[J].世界科学技术-中医药现代化, 2020, 22(4): 1330-1340.
XU G X, LUO L J, DENG Z H, et al. Research on acupointregularity of acupunctureand moxibustion treatment for BPH in recent 10 Years:A date mining based on clusteringand association rules[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology, 20, 22(4): 1330-1340.
何婷,杨硕,莫倩. 针灸治疗膀胱过度活动症的选穴用经特点与规律分析[J].世界科学技术-中医药现代化, 2018, 20(6): 960-966.
HE T, YANG S, MO Q. Analysis of the characteristics and rules of acupoints selection of acupuncture and moxibustion treatment of overactivitybladder[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2018, 20(6): 960-966.
张平薛,寇文平. 针刺次髎穴改善肛肠术后疼痛水肿的效果及相关疼痛介质、炎性因子水平的变化 [J].中华中医药学刊, 2022, 40(10): 87-90.
ZHANG P X, KOU W P. Effect of acupuncture at Ciliao(BL32)on pain and edema after anorectal surgery and changes in levels ofrelated pain mediators and inflammatory factors [J]. Chinese Archives of Traditional Chinese Medicine, 202, 40(10): 87-90.
黎文献,薛长利,黎建海. 针灸简易取穴法[M]. 广州: 科学普及出版社广州分社, 1988.
LI W X, XUE C L, LI J H. Simple acupuncture point selection [M]. Guangzhou: Science Popularization Press Guangzhou Branch, 1988.
杨文婷, 胡慧. 胡慧教授关于八髎穴应用的临床经验[J]. 北京中医药大学学报(中医临床版), 2013, 20(1): 60-61.
YANG W T, HU H. Professor Hu Hui's clinical experience on the application of Baliao Point [J]. Journal of Beijing University of Chinese Medicine (Clinical Edition of Traditional Chinese Medicine), 2013, 20(1): 60-61.
KIM K C,CHO H C,JANG T J,et al. Automatic detection and segmentation of lumbar vertebrae from X-ray images for compression fracture evaluation[J]. Comput Methods Programs Biomed, 2021, 200: 105833.
FENG Q F,HOU Y H,HOU W G,et al. The effects of acupuncture on bladder interstitial cells of cajal excitability in rats with overactive bladder[J]. Evid Based Complement Alternat Med, 2013, 2013: 261217.
李雯,金珠,刘建党,等. 电针对间质性膀胱炎大鼠脊髓背角p-ERK1/2和c-fos表达的影响[J].针刺研究, 2021, 46(5): 375-379.
LI W, JIN Z, LIU J D, et al. Effect of electroacupuncture on expressionof p-ERK1/2 and c-fos in spinal dorsal horn in rats with interstitial cystitis [J]. Acupuncture Research, 21, 46(5) : 375-379.
袁涌昊,赵若曦,夏庆昌,等.关于实验用大鼠腧穴定位的争议[J].时珍国医国药, 2022, 33(11): 2714-2715.
YUAN Y H, ZHAO R X, XIA Q C, et al. The controversy about the location of experimental rats'acupoints —Taking Shenque as an example[J].Lishizhen Medicine and Materia Medica Research, 2022,33 (11) : 2714-2715.
0
浏览量
0
下载量
0
CSCD
0
CNKI被引量
关联资源
相关文章
相关作者
相关机构