Methods： To explore the differences of volatile components in pine wood for

Poria cocos

planting before and after planting.

Methods

2

Pine samples before and after planting

Poria cocos

were separately extracted with petroleum ether， and the residues were extracted with methanol to obtain petroleum ether and methanol extracts. The volatile components in the extracts were analyzed by headspace-gas chromatography-mass spectrometry （HS-GC-MS）. The relative content of each component was calculated by peak area normalization method. The structures of the different compounds were confirmed through databases. Significantly different compounds were confirmed by OPLS-DA.

Results

2

A total of 130 chemical components were detected in pine samples before and after planting， of which the relative contents of 125 components changed before and after planting. Compared with the volatile components of pine samples before planting， the relative contents of 79 components of pine samples after planting were decreased， while the relative contents of 46 components were increased， of which the relative contents of 24 components were significantly decreased， while the relative contents of 9 components were significantly increased ［variable importance in the projection （VIP） > 1 or |p （corr）| >0.58 in S-plots］. The components that significantly decreased in the pine samples after planting were mainly acids， esters， alkanes， alkenes and ethers.

Conclusion

2

Compared with the pine samples before planting， the 79 components whose relative contents were decreased after planting， especially the 24 components whose relative contents were decreased significantly， may be the nutrients absorbed and utilized from pines during the growth of

Poria cocos

. The research results provide scientific basis for the pine replacement in planting

Poria cocos

and the protection of pine resources.