1.上海中医药大学中药学院(上海 201203)
2.上海中医药大学中药现代制剂技术教育部工程研究中心(上海 201203)
3.中药提取安徽省技术创新中心(安徽 亳州 236800)
4.安徽济人药业有限公司(安徽 亳州 236800)
金诚,女,在读博士生,主要从事中药制剂技术研究
吴飞,副研究员,硕士生导师;E-mail:wufei_shutcm@126.com
林晓,教授,博士生导师;E-mail: shutcmlx@163.com
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金诚,杜若飞,王权等.HPLC法同时测定康儿灵颗粒中新橙皮苷二氢查耳酮和纽甜的含量[J].上海中医药大学学报,2023,37(05):47-54.
JIN Cheng,DU Ruofei,WANG Quan,et al.Simultaneous determination of neohesperidin dihydrochalcone and neotame in Kang'erling Granules by HPLC method[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(05):47-54.
金诚,杜若飞,王权等.HPLC法同时测定康儿灵颗粒中新橙皮苷二氢查耳酮和纽甜的含量[J].上海中医药大学学报,2023,37(05):47-54. DOI: 10.16306/j.1008-861x.2023.05.006.
JIN Cheng,DU Ruofei,WANG Quan,et al.Simultaneous determination of neohesperidin dihydrochalcone and neotame in Kang'erling Granules by HPLC method[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(05):47-54. DOI: 10.16306/j.1008-861x.2023.05.006.
目的,2,建立一种同时测定康儿灵颗粒中新橙皮苷二氢查耳酮(NHDC)和纽甜含量的高效液相色谱(HPLC)分析方法。,方法,2,采用Agilent Zorbax SB-Aq(4.6 mm×250 mm)色谱柱进行分离检测,以乙腈-水(21∶79)为流动相,流速为1 mL/min,检测波长为218 nm,柱温为30 ℃。,结果,2,NHDC和纽甜分别在1.89~189.00 μg/mL和4.86~485.60 μg/mL范围内线性关系良好(,r,=0.999 9),平均加样回收率分别为97.59%、98.01%,,RSD,<,3.00%。,结论,2,该方法重复性好、准确性高、操作简便,可用于食品、医药行业中NHDC和纽甜的质量控制。
Objective: A high performance liquid chromatography (HPLC) method was developed for simultaneous determination of neohesperidin dihydrochalcone(NHDC) and neotame in Kang'erling Granules.,Methods,2,The Agilent Zorbax SB-Aq (4.6 mm×250 mm) was used for separation and detection, and the mobile phase consisted of acetonitrile-water(21∶79)at a flow rate of 1 mL/min. The detective wavelength was 218 nm and the column temperature was 30 ℃.,Results,2,NHDC and neotame had good linear relationships (,r,=0. 999 9) within the range of 1.89~189.00 μg/mL and 4.86~485.60 μg/mL respectively, whose average recovery rates were 97.59% and 102.41% respectively, and ,RSD,<,3.00%.,Conclusion,2,This method has good repeatability, high accuracy and simple operation, and can be used for the quality control of NHDC and neotame in food and pharmaceuticals industry.
高效液相色谱新橙皮苷二氢查耳酮纽甜
high performance liquid chromatographyneohesperidin dihydrochalconeneotame
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