三种广藿香油的化学成分及非靶向代谢组学分析

许玮华; 李晓锋; 彭涛兴; 王冰; 吴欢; 黄永焯

doi:10.16306/j.1008-861x.2023.05.003

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三种广藿香油的化学成分及非靶向代谢组学分析

中药分析专栏 | 更新时间：2023-12-02

- 三种广藿香油的化学成分及非靶向代谢组学分析
- Chemical composition and metabolomis analysis of non⁃targeted metabolites of three patchouli oils
- 上海中医药大学学报 2023年37卷第5期页码：19-30
- 作者机构：
  
  1.安徽中医药大学药学院（安徽　合肥　230012）
  2.中国科学院上海药物研究所（上海　201203）
  3.南通市海门长三角药物高等研究院（江苏　南通　226133）
- 作者简介：
  
  许玮华，女，在读硕士生，主要从事中药制剂研究
  王冰，副研究员，硕士生导师；E-mail：bwang@simm.ac.cn
  黄永焯，研究员，博士生导师；E-mail：yzhuang@simm.ac.cn
- 基金信息：
  
  中国科学院药物创新研究院自主部署项目(CASIMM0120184002)
- DOI：10.16306/j.1008-861x.2023.05.003
  中图分类号：
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许玮华,李晓锋,彭涛兴等.三种广藿香油的化学成分及非靶向代谢组学分析[J].上海中医药大学学报,2023,37(05):19-30.

XU Weihua,LI Xiaofeng,PENG Taoxing,et al.Chemical composition and metabolomis analysis of non⁃targeted metabolites of three patchouli oils[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(05):19-30.
许玮华,李晓锋,彭涛兴等.三种广藿香油的化学成分及非靶向代谢组学分析[J].上海中医药大学学报,2023,37(05):19-30. DOI： 10.16306/j.1008-861x.2023.05.003.

XU Weihua,LI Xiaofeng,PENG Taoxing,et al.Chemical composition and metabolomis analysis of non⁃targeted metabolites of three patchouli oils[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(05):19-30. DOI： 10.16306/j.1008-861x.2023.05.003.

摘要

目的,2,系统评价不同来源广藿香油的区别与质量，为后续广藿香油的应用研究提供可靠的实验基础。,方法,2,选择水蒸气蒸馏法和超临界萃取法制备广藿香挥发油，并采用气相色谱-火焰离子化检测器（GC-FID）和气质联用色谱仪（GC-MS）定量和定性分析两种自制挥发油和市售广藿香油的区别；并应用超高效液相色谱-质谱（UPLC-MS）技术，对3种不同来源的广藿香油进行非靶向代谢组学研究。,结果,2,3种广藿香油的化学成分大约有15种，主要包括倍半萜类、黄酮类等各类化合物。3组的组间差异性代谢物共有184种，超临界萃取与水蒸气蒸馏提取的挥发油存在显著差异，超临界萃取与市售的水蒸气蒸馏提取的挥发油差异较小，不同批次的市售广藿香油代谢物含量无明显差异。,结论,2,自制广藿香油的化学成分和代谢成分与市售基本一致，市售的广藿香油更稳定，为后续的药物研发和应用提供科学依据。

Abstract

Objective： To systematically and comprehensively evaluate the difference and quality of patchouli oils from different sources， in order to provide a scientific and reliable experimental basis for subsequent studies on the application of patchouli oil.,Methods,2,Steam distillation and supercritical extraction were chosen to prepare patchouli oil， gas chromatography-flame ionization detection （GC-FID） and gas chromatography-mass spectrometry （GC-MS） were used to quantitatively and qualitatively analyze the differences between the two lab-made volatile oils and marketed patchouli oil. The ultra-high performance liquid chromatography-mass spectrometry （UPLC-MS） technique was also applied to perform a non-targeted metabolomics study on three different sources of patchouli oils.,Results,2,There approximately 15 chemical components of the three patchouli oils， mainly including sesquiterpenoids and flavonoids and other compounds. There were 184 differential metabolites in three groups. There were significant difference between the supercritical and steam distillation extracted volatile oils， and less difference between the supercritical and the marketed steam distillation extracted volatile oils， and no significant difference in the metabolite content of different batches of commercially available patchouli oils.,Conclusion,2,The chemical composition of lab-made patchouli oil is essentially the same as marketed. Marketed patchouli oil is more stable， so as to provide scientific basis for its subsequent drug development and application.

关键词

广藿香油GC-MS非靶向代谢组学化学成分差异代谢物

Keywords

patchouli oilGC-MSnon-targeted metabolomicschemical compositiondifferential metabolites

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