1.上海中医药大学交叉科学研究院(上海 201203)
2.淮北师范大学生命科学学院(安徽 淮北 235000)
邢一卓,在读硕士生,主要从事抗肿瘤中药药理学研究
张宏,研究员,博士生导师;E-mail: zhanghong@shutcm.edu.cn
扫 描 看 全 文
邢一卓,杨鸿轩,邓仪卿等.基于肿瘤微环境的三维多细胞共培养模型构建方法[J].上海中医药大学学报,2023,37(04):95-101.
XING Yizhuo,YANG Hongxuan,DENG Yiqing,et al.Construction methods of 3D multicellular co⁃culture model based on tumor microenvironment[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(04):95-101.
邢一卓,杨鸿轩,邓仪卿等.基于肿瘤微环境的三维多细胞共培养模型构建方法[J].上海中医药大学学报,2023,37(04):95-101. DOI: 10.16306/j.1008-861x.2023.04.012.
XING Yizhuo,YANG Hongxuan,DENG Yiqing,et al.Construction methods of 3D multicellular co⁃culture model based on tumor microenvironment[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(04):95-101. DOI: 10.16306/j.1008-861x.2023.04.012.
恶性肿瘤有很高的发病率和病死率。尽管研究开发了大量抗肿瘤药物,但其临床疗效并不如意,这与复杂的肿瘤微环境(TME)密切相关。传统单一类型细胞的二维(2D)培养模型难以模拟TME空间结构中各种细胞的相互作用,阻碍了基于真实世界的药物研发。体外多细胞共培养的三维(3D)肿瘤球模型由于能更好地模拟体内TME,已逐渐成为最有前景的肿瘤生物学及抗肿瘤药物研究方法之一。中药具有多成分、多层次、多靶点协同发挥抗肿瘤作用的特点,单一类型细胞的2D模型往往不能反映中药整体作用,更适合采用多细胞共培养3D肿瘤球模型研究。综述了多细胞共培养3D模型的分类及构建方法。
Malignant tumors have high morbidity and mortality. Although a large number of anti-tumor drugs have been developed, their clinical efficacy is unsatisfactory, which is closely related to the complex tumor microenvironment (TME). It is difficult to simulate the interaction of various cells in the spatial structure of TME by traditional two-dimensional (2D) culture models of a single cell type, which hinders the drug research and development based on the real world. ,In vitro ,multicellular co-culture three-dimensional (3D) tumor spheroid models have gradually become the most promising approaches for tumor biology and anti-tumor drug research because it can better simulate ,in vivo, TME. Traditional Chinese medicine has the characteristics of synergistic anti-tumor effects through multi-component, multi-level and multi-target. However, 2D models of a single cell type often cannot reflect the overall effects of traditional Chinese medicine, making multicellular co-culture 3D tumor spheroid models more suitable for its research. This article reviews the classification and construction methods of 3D multicellular co-culture models.
多细胞共培养肿瘤球微流控肿瘤微环境
multicellularco-culturetumor spheroidmicrofluidicstumor microenvironment
SIEGEL R L, MILLER K D, FUCHS H E,et al. Cancer statistics, 2022[J]. CA Cancer J Clin, 2022, 72(1): 7-33.
WU T, DAI Y. Tumor microenvironment and therapeutic response[J]. Cancer Lett, 2017, 387: 61-68.
EDMONDSON R, BROGLIE J J, ADCOCK A F,et al. Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors[J]. Assay Drug Dev Technol,2014, 12(4): 207-218.
DUVAL K, GROVER H,HAN L H, et al. Modeling Physiological Events in 2D vs. 3D Cell Culture[J]. Physiology (Bethesda),2017, 32(4): 266-277.
ISHIGURO T, OHATA H,SATO A,et al. Tumor-derived spheroids: Relevance to cancer stem cells and clinical applications[J]. Cancer Sci, 2017, 108(3): 283-289.
CALPE B, KOVACS W J. High-throughput screening in multicellular spheroids for target discovery in the tumor microenvironment[J]. Expert Opin Drug Discov, 2020, 15(8): 955-967.
ZANONI M,PIGNATTA S,ARIENTI C,et al. Anticancer drug discovery using multicellular tumor spheroid models[J]. Expert Opin Drug Discov, 2019, 14(3): 289-301.
BADEKILA A K,KINI S, JAISWAL A K. Fabrication techniques of biomimetic scaffolds in three-dimensional cell culture: A review[J]. J Cell Physiol, 2021, 236(2): 741-762.
HAMILTON G. Multicellular spheroids as an in vitro tumor model[J]. Cancer Lett, 1998, 131(1): 29-34.
ZHUANG P,CHIANG Y H,FERNANDA M S,et al. Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment[J]. Int J Bioprint, 2021, 7(4): 444.
VAN DUINEN V,TRIETSCH S J,JOORE J,et al. Microfluidic 3D cell culture: from tools to tissue models[J]. Curr Opin Biotechnol, 2015, 35: 118-126.
WANG J, CHENG Y,YU Y,et al. Microfluidic Generation of Porous Microcarriers for Three-Dimensional Cell Culture[J]. ACS Appl Mater Interfaces, 2015, 7(49): 27035-27039.
KOHL Y,BIEHL M,SPRING S,et al. Microfluidic In Vitro Platform for (Nano)Safety and (Nano)Drug Efficiency Screening[J]. Small, 2021, 17(15): e2006012.
SACKMANN E K,FULTON A L, BEEBE D J. The present and future role of microfluidics in biomedical research[J]. Nature,2014, 507(7491): 181-189.
BEEBE D J,MENSING G A, WALKER G M. Physics and applications of microfluidics in biology[J]. Annu Rev Biomed Eng, 2002, 4: 261-286.
WHITESIDES G M. The origins and the future of microfluidics[J]. Nature, 2006, 442(7101): 368-373.
WEISWALD L B,RICHON S,VALIDIRE P,et al. Newly characterised ex vivo colospheres as a three-dimensional colon cancer cell model of tumour aggressiveness[J]. Br J Cancer,2009, 101(3): 473-482.
ACHILLI T M,MEYER J, MORGAN J R. Advances in the formation, use and understanding of multi-cellular spheroids[J]. Expert Opin Biol Ther, 2012, 12(10): 1347-1360.
RYU N E,LEE S H, PARK H. Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells[J]. Cells,2019, 8(12): 1620.
FOTY R. A simple hanging drop cell culture protocol for generation of 3D spheroids[J]. J Vis Exp, 2011, 51: e2720.
HUANG S W,TZENG S C,CHEN J K,et al. A Dynamic Hanging-Drop System for Mesenchymal Stem Cell Culture[J]. Int J Mol Sci, 2020, 21(12): 4298.
HUANG B W, GAO J Q. Application of 3D cultured multicellular spheroid tumor models in tumor-targeted drug delivery system research[J]. J Control Release, 2018, 270: 246-259.
SUN B,ZHAO Y,WU W,et al. A superhydrophobic chip integrated with an array of medium reservoirs for long-term hanging drop spheroid culture[J]. Acta Biomater,2021,135:234-242.
KAMATAR A,GUNAY G, ACAR H. Natural and Synthetic Biomaterials for Engineering Multicellular Tumor Spheroids[J]. Polymers (Basel), 2020, 12(11): 2506.
LU H, STENZEL M H. Multicellular Tumor Spheroids (MCTS) as a 3D In Vitro Evaluation Tool of Nanoparticles[J]. Small,2018, 14(13): e1702858.
ZARE M, BIGHAM A, ZARE M, et al. pHEMA: An Overview for Biomedical Applications[J]. Int J Mol Sci, 2021, 22(12): 6376.
HOLLAND G,PANDIT A,SANCHEZ-ABELLA L,et al. Artificial Cornea: Past, Current, and Future Directions[J]. Front Med (Lausanne), 2021, 8: 770780.
KHODADADI YAZDI M, TAGHIZADEH A, TAGHIZADEH M,et al. Agarose-based biomaterials for advanced drug delivery[J]. J Control Release, 2020, 326: 523-543.
JIANG C,LIU Z,CHENG D,et al. Agarose degradation for utilization: Enzymes, pathways, metabolic engineering methods and products[J]. Biotechnol Adv, 2020, 45: 107641.
MA R,MANDELL J,LU F,et al. Do Patient-derived Spheroid Culture Models Have Relevance in Chondrosarcoma Research?[J]. Clin Orthop Relat Res, 2021, 479(3): 477-490.
IVANOV D P,PARKER T L,WALKER D A,et al. In vitro co-culture model of medulloblastoma and human neural stem cells for drug delivery assessment[J]. J Biotechnol, 2015, 205: 3-13.
IVANOV D P,PARKER T L,WALKER D A,et al. Multiplexing spheroid volume, resazurin and acid phosphatase viability assays for high-throughput screening of tumour spheroids and stem cell neurospheres[J]. PLoS One, 2014, 9(8): e103817.
KHAWAR I A, PARK J K, JUNG E S, et al. Three Dimensional Mixed-Cell Spheroids Mimic Stroma-Mediated Chemoresistance and Invasive Migration in hepatocellular carcinoma[J]. Neoplasia, 2018, 20(8): 800-812.
GAO W, WU D, WANG Y,et al. Development of a novel and economical agar-based non-adherent three-dimensional culture method for enrichment of cancer stem-like cells[J]. Stem Cell Res Ther, 2018, 9(1): 243.
HAISLER W L, TIMM D M, GAGE J A, et al. Three-dimensional cell culturing by magnetic levitation[J]. Nat Protoc, 2013, 8(10): 1940-1949.
HENSLEE E A, DUNLOP C M, DE MEL C M, et al. DEP-Dots for 3D cell culture: low-cost, high-repeatability, effective 3D cell culture in multiple gel systems[J]. Sci Rep, 2020, 10(1): 14603.
CHEN B,WU Y,AO Z,et al. High-throughput acoustofluidic fabrication of tumor spheroids[J]. Lab Chip, 2019, 19(10):1755-1763.
KINGSLEY D M,ROBERGE C L,RUDKOUSKAYA A,et al. Laser-based 3D bioprinting for spatial and size control of tumor spheroids and embryoid bodies[J]. Acta Biomater, 2019, 95: 357-370.
LERMAN M J,LEMBONG J,GILLEN G,et al. 3D printing in cell culture systems and medical applications[J]. Appl Phys Rev,2018, 5(4): 041109.
NATH S, DEVI G R. Three-dimensional culture systems in cancer research: Focus on tumor spheroid model[J]. Pharmacol Ther, 2016, 163: 94-108.
0
浏览量
73
下载量
0
CSCD
0
CNKI被引量
关联资源
相关文章
相关作者
相关机构