黄慈颗粒通过改善神经再生损伤及胶质增生缓解5⁃FU诱导的小鼠认知障碍

王杰; 贾茹; 马福祺; 荣汶青; 李静茹; 冯琴

doi:10.16306/j.1008-861x.2022.S1.031

您当前的位置：

首页 >

文章列表页 >

黄慈颗粒通过改善神经再生损伤及胶质增生缓解5⁃FU诱导的小鼠认知障碍

实验研究 | 更新时间：2022-08-29

- 黄慈颗粒通过改善神经再生损伤及胶质增生缓解5⁃FU诱导的小鼠认知障碍
- Huangci Granules alleviate 5⁃FU induced cognitive impairment in mice through improving neuroregenerative damage and gliosis
- 上海中医药大学学报 2022年36卷第S1期页码：124-129
- 作者机构：
  
  1.上海中医药大学附属曙光医院肿瘤科，肿瘤研究所（上海　201203）
  2.上海中医药大学附属曙光医院肝病研究所（上海　　201203）
  3.陕西中医药大学（陕西　咸阳　712046）
- 作者简介：
  
  王杰，男，博士，助理研究员，主要从事认知功能障碍的基础和临床研究；E-mail：wangjieyct@163.com
- 基金信息：
  
  上海中医药大学附属曙光医院四明青年基金(SGKJ-202101)
- DOI：10.16306/j.1008-861x.2022.S1.031
  中图分类号：
扫描看全文
王杰,贾茹,马福祺等.黄慈颗粒通过改善神经再生损伤及胶质增生缓解5⁃FU诱导的小鼠认知障碍[J].上海中医药大学学报,2022,36(S1):124-129.

WANG Jie,JIA Ru,MA Fuqi,et al.Huangci Granules alleviate 5⁃FU induced cognitive impairment in mice through improving neuroregenerative damage and gliosis[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):124-129.
王杰,贾茹,马福祺等.黄慈颗粒通过改善神经再生损伤及胶质增生缓解5⁃FU诱导的小鼠认知障碍[J].上海中医药大学学报,2022,36(S1):124-129. DOI： 10.16306/j.1008-861x.2022.S1.031.

WANG Jie,JIA Ru,MA Fuqi,et al.Huangci Granules alleviate 5⁃FU induced cognitive impairment in mice through improving neuroregenerative damage and gliosis[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):124-129. DOI： 10.16306/j.1008-861x.2022.S1.031.

摘要

目的,2,观察黄慈颗粒对5-氟尿嘧啶（5-FU）诱导的小鼠认知功能障碍的作用及其机制。,方法,2,用5-FU干预小鼠诱导认知功能障碍模型，随机将小鼠分为对照组、模型组（5-FU， 60 mg/kg）、黄慈颗粒低剂量组（1.8 g/kg）和高剂量组（5.4 g/kg）。利用旷场实验和新旧事物识别实验观察各组小鼠运动能力及记忆能力的变化；利用免疫组化技术考察小鼠神经再生及胶质细胞活化情况。,结果,2,①各组小鼠运动能力未见明显差异；②与对照组相比，模型小鼠的记忆能力显著下降（,P,<,0.05），而黄慈颗粒能够显著改善小鼠记忆能力的受损（,P,<,0.05）；③与对照组相比，模型组小鼠海马齿状回颗粒下层和侧脑室室管膜下层双皮质素（DCX）的表达显著下降（,P,<,0.05），而低、高剂量黄慈颗粒均可显著提高DCX的表达（,P,<,0.05）；④与对照组相比，模型组小鼠海马区小胶质细胞及星形胶质细胞显著增生及活化（,P,<,0.05），而黄慈颗粒能够显著减少海马区小胶质细胞和星型胶质细胞的增殖及激活（,P,<,0.05）。,结论,2,黄慈颗粒可通过改善神经再生受损及胶质增生缓解5-FU诱导的小鼠认知障碍。

Abstract

Objective： To observe effect and mechanism of Huangci Granules on 5-fluorouracil （5-FU） induced cognitive impairment in mice.,Methods,2,The mice were randomly divided into control group， model group （5-FU， 60 mg/kg）， low-dose Huangci Granules group （1.8 g/kg） and high-dose Huangci Granules group （5.4 g/kg）. The changes of motor ability and memory ability were observed by open field test and new and old objects recognition test. The neurogenesis and gliosis in the brain of mice were investigated by immunohistochemistry in each group.,Results,2,①There was no significant difference in the motor ability of mice in each group. ②Compared to the control group， the memory ability of the model mice decreased significantly （,P,<,0.05）， and Huangci Granules could significantly improve the impairment of memory ability in mice （,P,<,0.05）. ③Compared to the control group， the expression of Doublecortin （DCX） in the subgranule zone of the dentate gyrus and subventricular zone of the lateral ventricle decreased significantly in mice of the model group （,P,<,0.05）， and both low dose and high dose Huangci Granules could significantly increase the expression of DCX （,P,<,0.05）. ④Compared to the control group， microglia and astrocytes in the hippocampus of the model group significantly proliferated and activated （,P,<,0.05）， and Huangci Granules could significantly reduce the proliferation and activation of microglia and astrocytes in the hippocampus （,P,<,0.05）.,Conclusion,2,Huangci Granules can alleviate 5-FU induced cognitive impairment in mice through improving nerve regeneration injury and gliosis.

关键词

黄慈颗粒5-氟尿嘧啶神经再生胶质增生化疗相关认知障碍

Keywords

Huangci Granules5-fluorouracilnerve regenerationgliosischemotherapy-related cognitive impairment

references

MILLER K D，NOGUEIRA L，MARIOTTO A B，et al.Cancer treatment and survivorship statistics， 2019［J］.CA Cancer J Clin， 2019， 69（5）： 363-385.

DAS A，RANADIVE N，KINRA M，et al.An overview on chemotherapy-induced cognitive impairment and potential role of antidepressants［J］.Curr Neuropharmacol， 2020， 18（9）： 838-851.

NGUYEN L D，EHRLICH B E.Cellular mechanisms and treatments for chemobrain： insight from aging and neurodegenerative diseases［J］.EMBO Mol Med，2020， 12（6）： e12075.

SEKERES M J，BRADLEY-GARCIA M，MARTINEZ-CANABAL A，et al.Chemotherapy-induced cognitive impairment and hippocampal neurogenesis： a review of physiological mechanisms and interventions［J］.Int J Mol Sci，2021， 22（23）： 12697.

RUNMMEL N G，CHAISWING L，BONDADA S，et al.Chemotherapy-induced cognitive impairment： focus on the intersection of oxidative stress and TNFalpha［J］.Cell Mol Life Sci， 2021， 78（19-20）： 6533-6540.

SCHROYEN G，BLOMMAERT J，VAN W D，et al.Neuroinfla-mmation and its association with cognition， neuronal markers and peripheral inflammation after chemotherapy for breast cancer［J］.Cancers （Basel），2021， 13（16）： 4198.

JIA R，LIU N，CAI G，et al.Effect of PRM1201 combined with adjuvant chemotherapy on preventing recurrence and metastasis of stage Ⅲ colon cancer： a randomized， double-blind， placebo-controlled clinical trial［J］.Front Oncol， 2021， 11： 618793.

ENNACEUR A， DELACOUR J.A new one-trial test for neurobiological studies of memory in rats. 1： behavioral data［J］.Behav Brain Res， 1988， 31（1）： 47-59.

ANACKER C，HEN R.Adult hippocampal neurogenesis and cognitive flexibility-linking memory and mood［J］.Nat Rev Neurosci， 2017， 18（6）： 335-346.

WALGRAVE H，BALUSU S，SNOECK S，et al.Restoring miR-132 expression rescues adult hippocampal neurogenesis and memory deficits in Alzheimer’s disease［J］.Cell Stem Cell， 2021， 28（10）： 1805-1821.

NOKIA M S，ANDERSON M L，SHORS T J.Chemotherapy disrupts learning， neurogenesis and theta activity in the adult brain［J］.Eur J Neurosci， 2012， 36（11）： 3521-3530.

SIRICHOAT A，KRUSRI S，SUWANNOKOT K，et al.Melatonin protects against methotrexate-induced memory deficit and hippocampal neurogenesis impairment in a rat model［J］.Biochem Pharmacol， 2019， 163： 225-233.

WINOCUR G，WOJTOWICZ J M，HUANG J，et al.Physical exercise prevents suppression of hippocampal neurogenesis and reduces cognitive impairment in chemotherapy-treated rats［J］. Psychopharmacology（Berl）， 2014， 231（11）： 2311-2320.

DISABOTO D J，QUAN N，GODBOUT J P.Neuroinflammation： the devil is in the details［J］.J Neurochem， 2016， 139： 136-153.

MONJE M L， TODA H，PALMER T D.Inflammatory blockade restores adult hippocampal neurogenesis［J］.Science， 2003， 302（5651）： 1760-1765.

KWAK J H，KIM S，YU N K，et al.Loss of the neuronal genome organizer and transcription factor CTCF induces neuronal death and reactive gliosis in the anterior cingulate cortex［J］.Genes Brain Behav，2021， 20（2）： e12701.

李林，魏海峰，张兰，等.中医“肾生髓，脑为髓海”现代生物学基础探讨［J］.中国中药杂志，2006， 17： 1397-1400.

浏览量

124

下载量

CSCD

CNKI被引量

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据