基于雌二醇变化探讨淫羊藿总黄酮胶囊对去卵巢大鼠骨丢失的作用机制

姜婵钰; 马锐; 李冠武; 施丹; 史晓

doi:10.16306/j.1008-861x.2022.04.011

您当前的位置：

首页 >

文章列表页 >

基于雌二醇变化探讨淫羊藿总黄酮胶囊对去卵巢大鼠骨丢失的作用机制

实验研究 | 更新时间：2022-08-29

- 基于雌二醇变化探讨淫羊藿总黄酮胶囊对去卵巢大鼠骨丢失的作用机制
- Study on mechanism of Epimedium Total Flavonoid Capsule on bone loss in ovariectomized rats based on changes in estradiol
- 上海中医药大学学报 2022年36卷第4期页码：72-77
- 作者机构：
  
  1.上海中医药大学附属岳阳中西医结合医院老年病科（上海　200437）
  2.上海中医药大学附属岳阳中西医结合医院放射科（上海　　200437）
- 作者简介：
  
  姜婵钰，女，在读硕士生，主要从事中西医结合防治老年退行性疾病研究
  姜婵钰，女，在读硕士生，主要从事中西医结合防治老年退行性疾病研究
  施丹，副主任医师，硕士生导师；E-mail：denny_125@sina.com
- 基金信息：
  
  国家自然科学基金资助项目(81874497);国家重点研发计划“中医药现代化研究”重点专项(2018YFC1704703);上海市中医专科专病联盟建设项目（ZY〔2018-2020〕-FWTX-4022）;上海市卫健委先进适宜技术项目(2019SY020)
- DOI：10.16306/j.1008-861x.2022.04.011
  中图分类号：
扫描看全文
姜婵钰,马锐,李冠武等.基于雌二醇变化探讨淫羊藿总黄酮胶囊对去卵巢大鼠骨丢失的作用机制[J].上海中医药大学学报,2022,36(04):72-77.

JIANG Chanyu,MA Rui,LI Guanwu,et al.Study on mechanism of Epimedium Total Flavonoid Capsule on bone loss in ovariectomized rats based on changes in estradiol[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(04):72-77.
姜婵钰,马锐,李冠武等.基于雌二醇变化探讨淫羊藿总黄酮胶囊对去卵巢大鼠骨丢失的作用机制[J].上海中医药大学学报,2022,36(04):72-77. DOI： 10.16306/j.1008-861x.2022.04.011.

JIANG Chanyu,MA Rui,LI Guanwu,et al.Study on mechanism of Epimedium Total Flavonoid Capsule on bone loss in ovariectomized rats based on changes in estradiol[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(04):72-77. DOI： 10.16306/j.1008-861x.2022.04.011.

摘要

目的,2,基于雌二醇的变化探讨淫羊藿总黄酮胶囊（EFC）对去卵巢骨质疏松模型大鼠的作用机制。,方法,2,将48只3月龄SD大鼠随机分为4组，即假手术组（SHAM组）、模型组（OVX组）、雌二醇组（E,2,组）、EFC组，每组12只。通过行双侧卵巢切除术造模，造模成功后E,2,组及EFC组每天分别给予相应药物干预，SHAM组以及OVX组每天给予等量的0.9%氯化钠溶液，连续12周。通过双能X线吸收测量法观察EFC对大鼠腰椎和股骨骨密度的影响，Micro-CT检测大鼠骨小梁微结构的变化，ELISA法检测大鼠血清中E,2,、Ⅰ型前胶原N端前肽（PINP）、Ⅰ型胶原羧基末端肽（β-CTX）含量变化。,结果,2,①与OVX组比较，EFC组血清E,2,含量显著增高（,P,<,0.01），但含量低于E,2,组；②与OVX组比较，EFC组血清β-CTX含量明显降低（,P,<,0.01），血清PINP含量上升（,P,<,0.05）；③EFC组腰椎骨密度、股骨骨密度较OVX组有所提高，但无统计学意义；④与OVX组比较，EFC组骨小梁体积分数增高（,P,<,0.05），骨小梁数明显增加（,P,<,0.01），骨小梁厚度有所提高，但无统计学意义，骨小梁分离度明显降低（,P,<,0.01）；⑤E,2,与PINP无显著相关性（,P,>,0.05），E,2,与β-CTX呈显著性负相关（,r,=-0.6596，,P,<,0.001）。,结论,2,EFC可能发挥雌激素样作用，通过调节血清骨代谢标志物含量以及骨小梁微结构的变化，改善骨密度从而起到防治骨质疏松的作用。

Abstract

Objective： To explore the mechanism of Epimedium Total Flavonoid Capsule（EFC）on ovariectomized osteoporosis model rats.,Methods,2,Forty-eight 3-month-old SD rats were randomly divided into 4 groups： SHAM operation group （Sham group）， model group（OVX group）， estradiol group（E,2, group）and EFC group， 12 rats in each group. The model was established by bilateral oophorectomy. After successful modeling， E,2, group and EFC group were given corresponding drug intervention respectively every day， while SHAM group and OVX group were given the same amount of 0.9% NaCl solution every day， each group was administered for 12 consecutive weeks. The effects of EFC on bone mineral density of lumbar vertebrae and femur in rats were observed by dual energy X-ray absorption measurement， the changes of trabecular microstructure in rats were detected by Micro-CT， and the content changes of E,2,， type Ⅰ procollagen amino terminal peptide（PINP） and type Ⅰ collagen carboxyl terminal peptide（β-CTX） in rats’ serum were detected by ELISA.,Results,2,①Compared with OVX group， serum E,2, content in EFC group was significantly increased （,P,<,0.01）， but lower than that in E,2, group. ②Compared with OVX group， serum β-CTX content in EFC group was significantly decreased （,P,<,0.01）， and serum PINP content was increased （,P,<,0.05）. ③The bone mineral density of lumbar vertebrae and femur in EFC group were higher than those in OVX group， but there was no statistical significance. ④Compared with OVX group， the volume fraction of bone trabecula in EFC group was increased （,P,<,0.05）， the number of bone trabecular was increased significantly （,P,<,0.01）， the thickness of bone trabecular was increased， but there was no statistical significance， and the separation degree of bone trabecular was decreased significantly （,P,<,0.01）. ⑤There was no significant correlation between E,2, and PINP （,P,>,0.05）， but there was a significant negative correlation between E,2, and β-CTX（,r,=-0.659 6， ,P,<,0.001）.,Conclusion,2,EFC may play an estrogen-like role in the prevention and treatment of osteoporosis by regulating the content of serum bone metabolism markers and the changes of bone trabecular microstructure and improving bone density.

关键词

淫羊藿总黄酮胶囊绝经后骨质疏松症雌二醇骨代谢

Keywords

Epimedium Total Flavonoid Capsulepostmenopausal osteoporosisestradiolbone metabolism

references

HARVEY N C， POOLE K E， RALSTON S H， et al. Towards a cure for osteoporosis： the UK Royal Osteoporosis Society （ROS） Osteoporosis Research Roadmap［J］. Arch Osteoporos， 2022， 17（1）： 12.

WANG L H， YU W， YIN X J， et al. Prevalence of Osteoporosis and Fracture in China： The China Osteoporosis Prevalence Study［J］. JAMA Netw Open， 2021， 4（8）： e2121106.

CHEUNG C L，ANG S B，CHADHA M，et al. An updated hip fracture projection in Asia：The Asian Federation of Osteoporosis Societies study［J］. Osteoporos Sarcopenia， 2018， 4（1）： 16-21.

SI L，WINZENBERG T M，JIANG Q， et al. Projection of osteoporosis-related fractures and costs in China：2010-2050［J］. Osteoporos Int， 2015， 26（7）： 1929-1937.

LI H X，XIAO Z S，QUARLES L D，et al. Osteoporosis：Mechanism， Molecular Target and Current Status on Drug Development［J］. Curr med chem， 2021， 28（8）： 1489-1507.

SCHINI M，PEEL N， TORONJO-URQUIZA L， et al. Evaluation of estimated glomerular function（eGFR）versus creatinine clearance（CrCl） to predict acute kidney injury when using zoledronate for the treatment of osteoporosis［J］. Osteoporos Int， 2022， 33（3）： 737-744.

ZHAO J F， XU J Y， XU Y E， et al. High-Throughput Metabolomics Method for Discovering Metabolic Biomarkers and Pathways to Reveal Effects and Molecular Mechanism of Ethanol Extract From Epimedium Against Osteoporosis［J］. Front Pharmacol， 2020， 11： 1318.

梁伟乔，钟诚，李宇明. 骨质疏松症的中医病因病机认识与治疗进展［J］. 中国骨质疏松杂志，2020， 26（1）： 135-139.

蔡毅，赵继荣，陈文，等. 基于“肾主骨生髓”理论综述骨髓间充质干细胞与绝经后骨质疏松的关系［J］. 中国骨质疏松杂志，2021， 27（11）： 1666-1670.

童伟伟，樊巧玲，谭峰. 天癸、BMSCs与PMOP的理论关系研究［J］. 中国中医基础医学杂志，2018， 24（5）： 610-613.

赖满香，廖利平，谭玮璐，等. “肾精-骨质疏松-骨髓间充质干细胞”理论探讨［J］. 中医杂志，2018， 59（2）： 100-103.

FU M，FANG L P，XIANG X，et al. Microarray analysis of circRNAs sequencing profile in exosomes derived from bone marrow mesenchymal stem cells in postmenopausal osteoporosis patients［J］. J Clin Lab Anal， 2022， 36（1）： e23916.

XU Q，CHEN G P，LIU X Q，et al. Icariin inhibits RANKL-induced osteoclastogenesis via modulation of the NF-κB and MAPK signaling pathways［J］. Biochem Biophys Res Commun，2019， 508（3）： 902-906.

XI H R， MA H P， YANG F F， et al. Total flavonoid extract of Epimedium herb increases the peak bone mass of young rats involving enhanced activation of the AC10/cAMP/PKA/CREB pathway［J］. J Ethnopharmacol， 2018， 223： 76-87.

WANG Z，WANG D，YANG D，et al. The effect of icariin on bone metabolism and its potential clinical application［J］. Osteoporos Int， 2018， 29（3）： 535-544.

ALMEIDA M， LAURENT M R， DUBOIS V， et al. Estrogens and Androgens in Skeletal Physiology and Pathophysiology［J］. Physiol Rev， 2017， 97（1）： 135-187.

浏览量

204

下载量

CSCD

CNKI被引量

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于PI3K/Akt信号通路探讨加味盆痛灵方对子宫内膜异位症模型大鼠的镇痛作用机制

益气清络方对类风湿关节炎患者炎症和骨破坏的影响

中医“肾主骨”理论与肾性骨病刍议