1.河北中医学院药学院(河北 石家庄 050091)
2.石家庄平安医院(河北 石家庄 050025)
3.河北省高校中药组方制剂应用技术研发中心(河北 石家庄 050091)
4.河北省中药组方制剂技术创新中心(河北 石家庄 050091)
孙吴倩,女,在读硕士生,主要从事中药制剂新剂型研究
黄怀鹏,研究员,硕士生导师;E-mail:huang71526@163.com
李春花,教授,博士生导师;E-mail:13803369966@163.com
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孙吴倩,张秀芳,忻晓东等.水蒸气蒸馏法和提取-共沸精馏耦合技术提取艾叶挥发油比较研究[J].上海中医药大学学报,2022,36(01):27-32.
SUN Wuqian,ZHANG Xiufang,XIN Xiaodong,et al.Comparative study on extraction of volatile oil from Artemisiae Argyi Folium by steam distillation and water extraction coupling rectification[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(01):27-32.
孙吴倩,张秀芳,忻晓东等.水蒸气蒸馏法和提取-共沸精馏耦合技术提取艾叶挥发油比较研究[J].上海中医药大学学报,2022,36(01):27-32. DOI: 10.16306/j.1008-861x.2022.01.005.
SUN Wuqian,ZHANG Xiufang,XIN Xiaodong,et al.Comparative study on extraction of volatile oil from Artemisiae Argyi Folium by steam distillation and water extraction coupling rectification[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(01):27-32. DOI: 10.16306/j.1008-861x.2022.01.005.
目的,2,比较水蒸气蒸馏法(SD)和提取-共沸精馏耦合技术(WER)提取艾叶挥发油的效果。,方法,2,分别采用SD和WER法提取艾叶挥发油,计算挥发油得率。采用气相色谱-质谱联用仪(GC-MS)对挥发油进行成分分析,比较两种提取方法制得的艾叶挥发油中成分及含量差异。,结果,2,①SD法提取挥发油得率为0.502 ml/100 g,WER法提取挥发油得率为0.596 ml/100 g,WER法提取艾叶挥发油的得率明显优于SD。②SD法提取的艾叶挥发油共分离出42种成分,其中桉叶油醇、β⁃石竹烯、α⁃松油醇分别占挥发油总量的10.80%、5.73%、3.18%。WER法提取的艾叶挥发油共分离出28种成分,桉叶油醇、β⁃石竹烯、α⁃松油醇分别占挥发油总量的19.15%、8.51%、4.61%,主要有效成分的含量均高于SD法。,结论,2,与SD法相比,WER法提取艾叶挥发油得率及有效成分含量较高,品相较好,更利于艾叶挥发油的提取。
Objective: To compare the effect of steam distillation (SD) and water extraction coupling rectification (WER) on the extraction of volatile oil from Artemisiae Argyi Folium.,Methods,2,The volatile oil from Artemisiae Argyi Folium was extracted by SD and WER methods, respectively, and the yield of volatile oil was calculated. The components of volatile oil were analyzed by gas chromatography-mass spectrometry (GC-MS). The components and contents of the volatile oil from Artemisiae Argyi Folium extracted by these two methods were compared.,Results,2,① The yield of volatile oil extracted by SD method was 0.502 ml/100 g, and that extracted by WER method was 0.596 ml/100 g. The yield of volatile oil extracted by WER method was significantly better than that extracted by SD method. ②A total of 42 components were isolated from the volatile oil of Artemisiae Argyi Folium extracted by SD method, among, cineole, β-caryophyllene and α-terpineol accounted for 10.80%, 5.73% and 3.18% of the total volatile oil respectively. A total of 28 components were isolated from the volatile oil of Artemisiae Argyi Folium extracted by WER method, and cineole, β-caryophyllene and α-terpineol accounted for 19.15%, 8.51% and 4.61% of the total volatile oil respectively, which suggested that the contents of main active components were higher than those by SD method.,Conclusion,2,Compared with SD method, WER method shows higher yield and effective component contents and better appearance, which is more conducive to the extraction of volatile oil from Artemisiae Argyi Folium.
艾叶挥发油水蒸气蒸馏法(SD)提取-共沸精馏耦合技术(WER)气相色谱-质谱联用(GC-MS)
Artemisiae Argyi Foliumvolatile oilsteam distillation(SD)water extraction coupling rectification(WER)gas chromatography-mass spectrometry (GC-MS)
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