Objective:,2,To investigate the therapeutic effects of Huganning Tablets on high fat diet induced non-alcoholic fatty liver disease(NAFLD) in mice, as well as its influence on the intestinal mucosal mechanical barrier function.,Methods:,2,Male C57BL/6J mice were randomly divided into the normal group, model group and Huganning Tablets(0.6 g·kg,-1,·d,-1,) group, 8 mice in each group.Except the normal group, the other mice were fed with high fat diet for 12 weeks to induce the NAFLD model.After modeling, the mice in each group were treated with the corresponding drug by intragastric administration for 4 weeks.After the last administration, the serum was taken and the colon and liver tissues were collected.The serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total cholesterol(TC), triglyceride(TG), low-density lipoprotein(LDL) and high-density lipoprotein(HDL) were detected by biochemical analyzer.The morphological changes of colon and liver tissues were observed after HE staining, and the lipid accumulation in liver tissue was observed after oil red O staining.The levels of TG and TC in liver tissue were detected by kits.The mRNA expressions of tumor necrosis factor-α(,TNF-α,), interleukin-1β(,IL-1β,), interleukin-6(,IL-6,), zonula occludens-1(,ZO-1,) 、,Claudin-1, and ,Occludin, were detected by PCR.The protein expressions of ZO-1, Claudin-1 and Occludin were detected by Western blot.,Results:,2,①The serum levels of ALT, AST, TG, TC, HDL and LDL in the model group were significantly higher than those in the normal group(,P,<,0.01) .Huganning Tablets could significantly decrease the serum levels of ALT, AST, TC, HDL and LDL in model mice(,P,<,0.05) .②Huganning Tablets could significantly reduce the TG level in the liver of model mice(,P,<,0.01), and obviously improve the hepatocyte steatosis and lipid accumulation.③The colon tissue of mice in the model group showed obvious crypt structure destruction, epithelial cell injury and inflammatory cell infiltration, and the mRNA expression levels of ,TNF-α,IL-1β, and ,IL-6, were significantly higher than those in the normal group(,P,<,0.05,P,<,0.01) .Huganning Tablets could significantly improve the pathological injury of colon tissue in model mice, and significantly reduce the mRNA expression levels of ,TNF-α, and ,IL-,6(,P,<,0.05) .④The mRNA expression levels of ,ZO-1, and ,Claudin-1, in colon tissue of mice in the model group were significantly lower than those in the normal group(,P,<,0.05,P,<,0.01), and the protein expression levels of ZO-1, Claudin-1 and Occludin were significantly lower than those in the normal group(,P,<,0.05) .Huganning Tablets could significantly increase the mRNA expression levels of ,ZO-1,Claudin-1, and ,Occludin, in colon tissue of model mice(,P,<,0.05), and up-regulate the protein expression levels of ZO-1 and Occludin(,P,<,0.05) .,Conclusion:,2,Huganning Tablets can improve the liver function and blood lipid metabolism of NAFLD model mice, and reduce the injury of liver and colon tissues.Its mechanism may be related to up-regulating the expressions of tight junction proteins ZO-1, Claudin-1 and Occludin in colon tissue and improving the intestinal mucosal mechanical barrier function.