健脾解毒方调节组蛋白去甲基化酶JMJD2C抑制结直肠癌转移
Jianpi Jiedu Recipe inhibits colorectal cancer metastasis through regulating histone demethylase JMJD2C
- 上海中医药大学学报 2021年35卷第6期 页码：42-48,65
- 作者机构：
1.上海中医药大学附属曙光医院肿瘤科（上海 201203）
2.上海中医药大学附属曙光医院肿瘤研究所（上海 201203）
3.上海中医药大学中西医结合研究院（上海 201203）
- 作者简介：
吴新楠，男，硕士，住院医师，主要从事中西医结合防治消化道肿瘤的临床与基础研究
季青，研究员，博士生导师；E-mail：ttt99118@hotmail.com
- 基金信息：国家自然科学基金资助项目(82074225;81830120;81573749)
DOI：10.16306/j.1008-861x.2021.06.007
中图分类号：
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吴新楠, 李瑞晓, 李昊泽, 等. 健脾解毒方调节组蛋白去甲基化酶JMJD2C抑制结直肠癌转移[J]. 上海中医药大学学报, 2021,35(6):42-48,65.
WU Xinnan, LI Ruixiao, LI Haoze, et al. Jianpi Jiedu Recipe inhibits colorectal cancer metastasis through regulating histone demethylase JMJD2C[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine, 2021,35(6):42-48,65.
吴新楠, 李瑞晓, 李昊泽, 等. 健脾解毒方调节组蛋白去甲基化酶JMJD2C抑制结直肠癌转移[J]. 上海中医药大学学报, 2021,35(6):42-48,65. DOI： 10.16306/j.1008-861x.2021.06.007.
WU Xinnan, LI Ruixiao, LI Haoze, et al. Jianpi Jiedu Recipe inhibits colorectal cancer metastasis through regulating histone demethylase JMJD2C[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine, 2021,35(6):42-48,65. DOI： 10.16306/j.1008-861x.2021.06.007.
摘要
目的：,2,探讨健脾解毒方调节组蛋白去甲基化酶JMJD2C发挥抗结直肠癌转移的作用机制。,方法：,2,①取24只裸鼠，尾静脉注射结直肠癌HCT116细胞，复制结直肠癌肺转移模型。造模7 d后，将裸鼠平分为4组，分别灌胃给予不同剂量（0、250、500、1 000 mg/kg）的健脾解毒方水煎液。药物干预28 d后，分离肺转移灶组织，免疫组化检测JMJD2C蛋白表达。②分别以HCT116和LoVo细胞为研究对象，给予不同浓度（0、50、100、200 μg/ml）健脾解毒方醇提液。药物干预48 h后，PCR检测,JMJD2C, mRNA表达，Western blot检测JMJD2C蛋白表达，免疫荧光检测JMJD2C的蛋白表达和细胞定位。,结果：,2,①健脾解毒方能够显著下调裸鼠结直肠癌肺转移灶中JMJD2C蛋白表达，且抑制作用呈剂量依赖性。②健脾解毒方能够显著下调两种结直肠癌细胞中JMJD2C的mRNA和蛋白表达，且100、200 μg/ml作用更显著。③健脾解毒方能够抑制JMJD2C蛋白在结直肠癌细胞胞核内的累积，且100、200 μg/ml作用更显著。,结论：,2,健脾解毒方能够抑制结直肠癌转移，作用机制可能与下调JMJD2C表达及其在细胞核的累积相关。
Abstract
Objective:,2,To explore the mechanism of Jianpi Jiedu Recipe regulating histone demethylase JMJD2C against colorectal cancer metastasis.,Methods:,2,①Twenty-four nude mice were taken and injected with colorectal cancer HCT116 cells via caudal vein, to replicate the lung metastasis model of colorectal cancer.Seven days after modeling, the nude mice were equally divided into four groups, and treated with Jianpi Jiedu Recipe decoction at different doses(0, 250, 500, 1 000 mg/kg) by intragastric administration.After drug treatment for 28days, the lung metastasis tissuewas isolated, andtheexpressionofJMJD2Cproteinwas detectedby immunohistochemistry.②HCT116 and LoVo cells were taken as the research objects, respectively.The cells were treated with alcohol extract of Jianpi Jiedu Recipe at different concentrations(0, 50, 100, 200 μg/ml) .After drug treatment for 48 hours, the expression of ,JMJD2C, mRNA was detected by PCR, the expression of JMJD2C protein was detected by Western blot, and the protein expression and cell localization of JMJD2C were detected by immunofluorescence.,Results:,2,①Jianpi Jiedu Recipe could significantly down-regulate the expression of JMJD2C protein in lung metastases of colorectal cancer in nude mice, and the inhibitory effect showed in a dose-dependent manner.②Jianpi Jiedu Recipe could significantly down-regulate the mRNA and protein expressions of JMJD2C in these two colorectal cancer cells, and Jianpi Jiedu Recipe at 100 and 200 μg/ml showed more significant effects.③Jianpi Jiedu Recipe could inhibit the accumulation of JMJD2C protein in the nucleus of colorectal cancer cells, and Jianpi Jiedu Recipe at 100 and 200 μg/ml showed more significant effects.,Conclusion:,2,Jianpi Jiedu Recipe can inhibit the metastasis of colorectal cancer, and the mechanism may be related to the down-regulation of JMJD2C expression and its accumulation in the nucleus.
关键词
健脾解毒方组蛋白去甲基化酶JMJD2C结直肠癌转移裸鼠
Keywords
Jianpi Jiedu Recipehistone demethylase JMJD2Ccolorectal cancermetastasisnude mouse
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