健脾补肾方对大肠癌术后患者肠道菌群的影响
Effects of Jianpi Bushen Formula on intestinal flora in postoperative patients with colorectal cancer
- 上海中医药大学学报 2021年35卷第6期 页码：22-30
- 作者机构：
1.北京中医药大学（北京 100029）
2.北京中医药大学东方医院肿瘤科（北京 100078）
3.宁海县中医医院医疗健康集团（浙江 宁波 315600）
- 作者简介：
谭雅彬，女，在读博士生，主要从事中西医结合防治恶性肿瘤的临床与基础研究。朱名扬，女，在读硕士生，主要从事中西医结合防治恶性肿瘤的临床与基础研究，为共同第一作者
左明焕，教授、主任医师，博士生导师；E-mail：zuomh@263.net。
冯宝约，硕士，医师；E-mail：Fun-baby@qq.com
- 基金信息：北京市科技计划项目(D161100005116004)
DOI：10.16306/j.1008-861x.2021.06.004
中图分类号：
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谭雅彬, 朱名扬, 王一同, 等. 健脾补肾方对大肠癌术后患者肠道菌群的影响[J]. 上海中医药大学学报, 2021,35(6):22-30.
TAN Yabin, ZHU Mingyang, WANG Yitong, et al. Effects of Jianpi Bushen Formula on intestinal flora in postoperative patients with colorectal cancer[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine, 2021,35(6):22-30.
谭雅彬, 朱名扬, 王一同, 等. 健脾补肾方对大肠癌术后患者肠道菌群的影响[J]. 上海中医药大学学报, 2021,35(6):22-30. DOI： 10.16306/j.1008-861x.2021.06.004.
TAN Yabin, ZHU Mingyang, WANG Yitong, et al. Effects of Jianpi Bushen Formula on intestinal flora in postoperative patients with colorectal cancer[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine, 2021,35(6):22-30. DOI： 10.16306/j.1008-861x.2021.06.004.
摘要
目的：,2,探讨健脾补肾方对大肠癌（CRC）术后脾肾阳虚证患者肠道菌群的影响。,方法：,2,纳入40例CRC术后脾肾阳虚证患者和10例健康志愿者，患者入组后给予健脾补肾方口服治疗，周期为14 d。健脾补肾方干预前后，评价并记录所有患者的相关临床症状评分，比较患者症状总积分的变化；采集健脾补肾方干预前后患者及健康志愿者的粪便样本，运用16SrDNA测序技术检测各样本中的肠道菌群，分析患者与健康志愿者的肠道菌群结构差异，比较健脾补肾方干预前后患者肠道菌群结构的变化。,结果：,2,①健脾补肾方干预后，CRC术后脾肾阳虚证患者的症状总积分较干预前显著降低（,P,<,0.05）。②受试患者与健康志愿者的肠道菌群结构在各分类水平上均存在差异，健脾补肾方干预前后患者的肠道菌群结构在各分类水平上均发生了改变。,结论：,2,CRC术后脾肾阳虚证患者的肠道菌群结构与健康人存在差异，健脾补肾方干预可改善患者的相关症状及肠道菌群结构，健脾补肾方改善CRC术后脾肾阳虚证患者相关症状的作用机制可能与改善患者的肠道微生态环境有关。
Abstract
Objective:,2,To investigate the effects of Jianpi Bushen Formula on intestinal flora in postoperative patients of colorectal cancer(CRC) with spleen-kidney yang deficiency syndrome.,Methods:,2,Forty postoperative patients of CRC with spleen-kidney yang deficiency syndrome and 10 healthy volunteers were included.After enrollment, the patients were orally treated with Jianpi Bushen Formula with a course of 14 days.Before and after intervention of Jianpi Bushen Formula, the relevant clinical symptom scores of all patients were evaluated and recorded, and the total symptom scores of the patients were compared.The feces specimens of the healthy volunteers and the patients before and after drug treatment were collected, the intestinal flora in all samples were detected by 16SrDNA sequencing technology, the differences of intestinal flora structure between the patients and the healthy volunteers were analyzed, and the intestinal flora structure of the patients before and after intervention of Jianpi Bushen Formula were compared.,Results:,2,①After intervention of Jianpi Bushen Formula, the total symptom score of CRC postoperative patients with spleen-kidney yang deficiency syndrome were significantly decreased compared with that before intervention(,P,<,0.05) .②There were differences in the intestinal flora structure at all classification levels between the patients and the healthy volunteers.Before and after intervention of Jianpi Bushen Formula, the intestinal flora structure of the patients changed at all classification levels.,Conclusion:,2,There are differences in the intestinal flora structure between the healthy people and the CRC postoperative patients with spleen-kidney yang deficiency syndrome.The intervention of Jianpi Bushen Formula can improve the relevant clinical symptoms and the intestinal flora structure of the patients.The mechanism of Jianpi Bushen Formula improving the relevant symptoms of CRC postoperative patients with spleen-kidney yang deficiency syndrome may be related to improving the intestinal microecological environment.
关键词
大肠癌术后脾肾阳虚证健脾补肾方肠道菌群
Keywords
colorectal cancerpostoperationspleen-kidney yang deficiency syndromeJianpi Bushen Formulaintestinal flora
references
SIEGEL R L，MILLER K D，FUCHS H E, et al.Cancer Statistics，2021[J].CA Cancer J Clin，2021，71(1)：7-33.
AI D M，PAN H F，HAN R B, et al.Using Decision Tree Aggregation with Random Forest Model to Identify Gut Microbes Associated with Colorectal Cancer[J].Genes，2019，10(2)：112.
GUYTON K，ALVERDY J C.The gut microbiota and gastrointestinal surgery[J].Nat Rev Gastroenterol Hepatol，2017，14(1)：43-54.
冯宝约.温阳健脾法治疗大肠癌术后患者中医证候变化与肠道菌群的关系研究[D].北京：北京中医药大学，2019.
WANG J，FENG W，TANG F, et al.Gut microbial transformation, a potential improving factor in the therapeutic activities of four groups of natural compounds isolated from herbal medicines[J]. Fitoterapia，2019，138：104293.
WONG S H，YU J.Gut microbiota in colorectal cancer：mechanisms of action and clinical applications[J].Nat Rev Gastroenterol Hepatol，2019，16(11)：690-704.
来岳标，陈玉亚，姚庆华.中西医治疗对肠癌患者肠道菌群失调影响的研究进展[J].浙江中西医结合杂志，2018，28(7)：604-606.
国家卫生计生委医政医管局，中华医学会肿瘤学分会.中国结直肠癌诊疗规范（2017年版）[J].中华普通外科学文献（电子版），2018，12(3)：145-159.
American Joint Committee on Cancer.AJCC Cancer Staging Manual[M].8th ed.New York：Springer Verlag，2017：255.
林洪生.恶性肿瘤中医诊疗指南[M].北京：人民卫生出版社，2014.
李灿东.中医诊断学[M].北京：中国中医药出版社，2018.
郑筱萸.中药新药临床研究指导原则（试行）[M].北京：中国医药科技出版社，2002.
王华中，吴玉华，曹文，等.健脾益肾、化瘀解毒法联合化疗治疗老年结直肠癌术后30例临床观察[J].中医临床研究，2018，10(2)：15-17.
李志发，罗超元，吴小兵.温肾健脾益气法联合足三里穴位埋线对腹腔镜直肠癌术后患者胃肠功能恢复及机体免疫功能影响[J].现代中西医结合杂志，2017，26(6)：580-583.
FENG Q，LIANG S，JIA H, et al.Gut microbiome development along the colorectal adenoma-carcinoma sequence[J].Nat Commun，2015，6：6528.
WIRBEL J，PYL P T，KARTAL E, et al.Meta-analysis of fecal metagenomes reveals global microbial signatures that are specific for colorectal cancer[J].Nat Med，2019，25(4)：679-689.
VANDEPUTTE D，FALONY G，VIEIRA-SILVA S, et al.Stool consistency is strongly associated with gut microbiota richness and composition, enterotypes and bacterial growth rates[J].Gut，2016，65(1)：57-62.
LIANG J Q，LI T，NAKATSU G, et al.A novel faecal Lachnoclostridium marker for the non-invasive diagnosis of colorectal adenoma and cancer[J].Gut，2020，69(7)：1248-1257.
COSTELLO M E，CICCIA F，WILLNER D, et al.Brief Report: Intestinal Dysbiosis in Ankylosing Spondylitis[J].Arthritis Rheumatol，2015，67(3)：686-691.
MONDOT S，DE WOUTERS T，DORÉ J, et al.The human gut microbiome and its dysfunctions[J].Dig Dis，2013，31(3-4)：278-285.
MA Q，LI Y，WANG J, et al.Investigation of gut microbiome changes in type 1 diabetic mellitus rats based on high-throughput sequencing[J].Biomed Pharmacother，2020，124：109873.
张巍远，王玉柳明，张宇坤，等.结肠癌患者术前肠道菌群特征与术后腹泻的关系研究[J].结直肠肛门外科，2020，26(3)：337-341.
KINOSHITA M，SUZUKI Y，SAITO Y.Butyrate reduces colonic paracellular permeability by enhancing PPARγ activation[J].Biochem Biophys Res Commun，2002，293(2)：827-831.
CANI P D，AMAR J，IGLESIAS M A, et al.Metabolic endotoxemia initiates obesity and insulin resistance[J].Diabetes，2007，56(7)：1761-1772.
SCHLUTER J，PELED J U，TAYLOR B P, et al.The gut microbiota is associated with immune cell dynamics in humans[J].Nature，2020，588(7837)：303-307.
CHAPUT N，LEPAGE P，COUTZAC C, et al.Baseline gut microbiota predicts clinical response and colitis in metastatic melanoma patients treated with ipilimumab[J].Ann Oncol，2017，28(6)：1368-1379.
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