Objective:,2,To explore the effects of Kushenol F on oxidative stress and autophagy in melanocytes.,Methods:,2,PIG1 cell line was used as the research object.The cells were divided into the control group, model group, positive control(berberine, 5 μmol/L) group and Kushenol F groups with low-(26 μmol/L), middle-(52 μmol/L) and high-(78 μmol/L) dose.Except the control group, the other groups were treated with 1.0 mmol/L hydrogen peroxide(H,2,O,2,) for 24 hours to induce oxidative stress injury.After H,2,O,2, stimulation, each drug group was treated with the corresponding drug for 24 hours.The cell viability was detected by CCK8 assay.The levels of reactive oxygen species(ROS), malondialdehyde(MDA) and glutathione(GSH) were detected by ELISA.The autophagosomes were observed by transmission electron microscope.The expression of E-cadherin was detected by immunofluorescence.The expressions of oxidative stress and autophagy related proteins were detected by Western blot, including Beclin-1, P62, microtubule-associated protein 1 light chain 3(LC3) and nuclear factor erythroid 2-related factor 2(Nrf2) .,Results:,2,①Compared with the model group, the cell viability of Kushenol F groups with different doses was significantly increased(,P,<,0.01), and the cell viability of high-dose group was significantly higher than that of low-dose group and positive control group(,P,<,0.05) .②Compared with the model group, the levels of ROS and MDA were significantly decreased(,P,<,0.05,P,<,0.01) and the level of GSH was significantly increased(,P,<,0.05,P,<,0.01) in the Kushenol F groups with different doses.The levels of ROS and MDA in the high-dose group were lower than those in the low-dose group and positive control group((,P,<,0.05,P,<,0.01), and the level of GSH in the high-dose group was higher than that in the low-dose group(,P,<,0.05) .③Compared with the model group, the number of autophagosomes in the Kushenol F groups with different doses was significantly increased(,P,<,0.05,P,<,0.01) ; and the number of autophagosomes in the middle-and high-dose groups was significantly more than that in the low-dose group(,P,<,0.05,P,<,0.01) .④The expression of E-cadherin in the Kushenol F groups with middle-and high-dose was significantly higher than that in the model group(,P,<,0.05,P,<,0.01), and was significantly higher than that in the low-dose group(,P,<,0.05,P,<,0.01) .⑤Compared with the model group, after treatment with Kushenol F at different doses, the protein expression levels of Beclin-1, LC3Ⅱ/LC3Ⅰ, Nrf2 and P62 were significantly increased(,P,<,0.05,P,<,0.01) .,Conclusion:,2,Kushenol F may up-regulate Nrf2-P62 signaling pathway, promote melanocyte autophagy, and thus resist oxidative stress injury.