小豆蔻明对人三阴性乳腺癌MDA-MB-231细胞葡萄糖摄取能力的影响

靳金美; 邱水平; 石海莲; 张德高; 吴晓俊

doi:10.16306/j.1008-861x.2021.04.008

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小豆蔻明对人三阴性乳腺癌MDA-MB-231细胞葡萄糖摄取能力的影响

实验研究 | 更新时间：2022-09-09

- 小豆蔻明对人三阴性乳腺癌MDA-MB-231细胞葡萄糖摄取能力的影响
- Effects of cardamonin on glucose uptake capacity in human triple negative breast cancer MDA-MB-231 cells
- 上海中医药大学学报 2021年35卷第4期页码：54-60
- 作者机构：
  
  1.上海市复方中药重点实验室，教育部中药标准化重点实验室，国家中医药管理局中药新资源与品质评价重点研究室，上海中医药大学中药研究所（上海　201203）
  2.上海交通大学医学院附属新华医院崇明分院药剂科/药物临床试验机构办公室（上海　202150）
- 作者简介：
  
  靳金美，女，硕士，主要从事中药及其活性成分作用机制研究
  石海莲，研究员，硕士生导师；E-mail：shihailian2003@163.com。
  张德高，主管药师；E-mail：decaux@163.com
- 基金信息：
  
  国家自然科学基金资助项目(81603354);上海市自然科学基金项目(21ZR1462800);上海市教委预算内项目(2020LK014);上海高校中药药效物质E研究院项目
- DOI：10.16306/j.1008-861x.2021.04.008
  中图分类号：
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靳金美, 邱水平, 石海莲, 等. 小豆蔻明对人三阴性乳腺癌MDA-MB-231细胞葡萄糖摄取能力的影响[J]. 上海中医药大学学报, 2021,35(4):54-60.

JIN Jinmei, QIU Shuiping, SHI Hailian, et al. Effects of cardamonin on glucose uptake capacity in human triple negative breast cancer MDA-MB-231 cells[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine, 2021,35(4):54-60.
靳金美, 邱水平, 石海莲, 等. 小豆蔻明对人三阴性乳腺癌MDA-MB-231细胞葡萄糖摄取能力的影响[J]. 上海中医药大学学报, 2021,35(4):54-60. DOI： 10.16306/j.1008-861x.2021.04.008.

JIN Jinmei, QIU Shuiping, SHI Hailian, et al. Effects of cardamonin on glucose uptake capacity in human triple negative breast cancer MDA-MB-231 cells[J]. Academic Journal of Shanghai University of Traditional Chinese Medicine, 2021,35(4):54-60. DOI： 10.16306/j.1008-861x.2021.04.008.

摘要

目的：,2,考察小豆蔻明对人三阴性乳腺癌MDA-MB-231细胞葡萄糖摄取能力的影响，并探讨其分子机制。,方法：,2,将MDA-MB-231细胞分为小豆蔻明不同浓度（0、10、20、40、80 μmol/L）组，分别予以相应浓度药物干预24 h。CCK-8法检测细胞活力，2-NBDG法检测细胞葡萄糖摄取能力，PCR法检测细胞葡萄糖转运体（,GLUT,）mRNA表达，Western blot法检测细胞GLUT蛋白表达。皮下接种MDA-MB-231细胞构建荷瘤裸鼠模型，小豆蔻明（0.3、1.0、3.0 mg/kg）腹腔注射给药4周后，分离荷瘤小鼠乳腺癌组织，Western blot法检测乳腺癌组织GLUT蛋白表达。,结果：,2,小豆蔻明（20、40、80 μmol/L）能剂量依赖性抑制MDA-MB-231细胞的活力（,P,<,0.01），且小豆蔻明（20 μmol/L）能够显著降低乳腺癌细胞的葡萄糖摄取能力（,P,<,0.05）。进一步研究发现，小豆蔻明（20 μmol/L）能够显著下调乳腺癌细胞,GLUT3,和,GLUT4, mRNA表达（,P,<,0.01）；小豆蔻明（40 μmol/L）能够显著下调乳腺癌细胞GLUT3蛋白表达（,P,<,0.01），小豆蔻明（10、20、40 μmol/L）能够显著下调乳腺癌细胞GLUT4蛋白表达（,P,<,0.01），但对GLUT1蛋白表达无明显影响（,P,>,0.05）。小豆蔻明（0.3、1.0、3.0 mg/kg）能够显著降低荷瘤裸鼠乳腺癌组织GLUT1蛋白表达（,P,<,0.01），而小豆蔻明（1.0、3.0 mg/kg）能够显著下调GLUT3蛋白表达（,P,<,0.01）。,结论：,2,小豆蔻明可能通过下调GLUT mRNA和蛋白表达，降低人三阴性乳腺癌MDA-MB-231细胞的活力和葡萄糖摄取能力。

Abstract

Objective:,2,To investigate the effects of cardamonin on the glucose uptake capacity in human triple negative breast cancer MDA-MB-231 cells and explore its molecular mechanism.,Methods:,2,MDA-MB-231 cells were divided into the cardamonin groups with different concentrations(0, 10, 20, 40, 80 μmol/L) .Each group was treated with cardamonin at the corresponding concentration for 24 hours.The cell viability was detected by CCK-8 assay.The glucose uptake capacity was evaluated by 2-NBDG assay.The mRNA expressions of glucose transporters(,GLUT,) were detected by PCR.The protein expressions of GULT were detected by Western blot.MDA-MB-231 cells were subcutaneously inoculated to construct tumor bearing nude mice model.After intraperitoneal injection of cardamonin(0.3, 1.0, 3.0 mg/kg) for 4 weeks, the breast cancer tissue of tumor bearing mice was isolated, and the expressions of GLUT proteins in the breast cancer tissue were detected by Western blot.,Results:,2,Cardamonin(20, 40, 80 μmol/L) significantly inhibited the viability of MDA-MB-231 cells in a dose-dependent manner(,P,<,0.01), and cardamonin at concentration of 20 μmol/L significantly reduced the glucose uptake capacity of breast cancer cells(,P,<,0.05) .Further studies showed that, cardamonin at concentration of 20 μmol/L significantly down-regulated the mRNA expressions of ,GLUT3, and ,GLUT4, in breast cancer cells(,P,<,0.01) .Cardamonin at concentration of 40 μmol/L significantly down-regulated the protein expression of GLUT3 in breast cancer cells(,P,<,0.01), cardamonin(10, 20, 40 μmol/L) significantly down-regulated the protein expression of GLUT4 in breast cancer cells(,P,<,0.01), but showed no obvious effect on GLUT1 protein expression(,P,>,0.05) .Cardamonin(0.3, 1.0, 3.0 mg/kg) significantly reduced the protein expression of GLUT1 in breast cancer tissue of tumor bearing mice(,P,<,0.01), while cardamonin(1.0, 3.0 mg/kg) significantly down-regulated the protein expression of GLUT3(,P,<,0.01) .,Conclusion:,2,Cardamonin may decrease the cell viability and glucose uptake capacity of human triple negative breast cancer MDA-MB-231 cells by down-regulating the mRNA and protein expressions of GLUT.

关键词

小豆蔻明乳腺癌细胞活力葡萄糖摄取葡萄糖转运体

Keywords

cardamoninbreast cancercell viabilityglucose uptakeglucose transporter

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