Objective:,2,To study the effects of short-term administration of cisplatin on syndrome and steroidogenesis in mice.,Methods:,2,Forty male ICR mice were randomly divided into the control group and cisplatin groups with different doses(3, 5, 10 mg·kg,-1,·d,-1,), 10 mice in each group.The drug intervention groups were intraperitoneally injected with cisplatin at the corresponding dose for 5 days.After last administration and withdrawal for 5 days, the signs(body mass, food intake, axillary temperature and claw color) of mice were observed, and the behavioral tests(grip, open field test and rotarod test) were performed.After observation period, the serum was taken, the kidney, heart, thymus and spleen were separated and the organ index was calculated, and the adrenal gland and testis were collected.The serum levels of corticosterone and testosterone were detected by ELISA.The morphological changes of adrenal gland and testis were observed under light microscope after HE staining.The expressions of genes and proteins related to steroid hormone synthetase and testosterone synthetase were detected by PCR and Western blot respectively.,Results:,2,①After administration for 5 days, the body mass, food intake, axillary temperature, horizontal movement and vertical movement in the cisplatin 10 mg·kg,-1,·d,-1, group were significantly decreased compared with those in the control group(,P,<,0.05,P,<,0.01) ; the axillary temperature of the cisplatin 3 and 5 mg·kg,-1,·d,-1, groups was significantly lower than that of the control group, while the r value of claw color was higher than that of the control group(,P,<,0.05,P,<,0.01) .After withdrawal for 5 days, the body mass, food intake, stay time, grip, axillary temperature, r value of claw color, horizontal movement and vertical movement in the cisplatin 10 mg·kg,-1,·d,-1, group were significantly decreased compared with those in the control group(,P,<,0.05,P,<,0.01) ; and the vertical movement of the cisplatin 3 and 5 mg·kg,-1,·d,-1, groups was also decreased compared with that of the control group(,P,<,0.01) .②Compared with the control group, cisplatin at dose of 10 mg·kg,-1,·d,-1, significantly inhibited the spleen, thymus and heart of mice, these organ indexes were decreased significantly(,P,<,0.05,P,<,0.01), while the kidney index was increased significantly(,P,<,0.01) ; cisplatin at dose of 5 mg·kg,-1,·d,-1, also inhibited the heart(,P,<,0.05) .③The morphology showed that, the cells in the glomerular zone of adrenal cortex swelled obviously in the cisplatin 10 mg·kg,-1,·d,-1, group, the arrangement of spermatogenic cells at all levels was slightly disordered, and the leydig cells of testis contracted obviously in the cisplatin 5 and 10 mg·kg,-1,·d,-1, groups.④Compared with the control group, the serum content of corticosterone was significantly increased in the cisplatin 10 mg·kg,-1,·d,-1, group(,P,<,0.01), while there was no significant change in serum testosterone.⑤Compared with the control group, the mRNA expressions of ,Star, and ,Cyp21a1, in the cisplatin 3 mg·kg,-1,·d,-1, group was inhibited(,P,<,0.05), while the mRNA expressions of ,Star,Cyp11b1, and ,Cyp21a1, and the protein expressions of StAR, CYP21A2 and CYP11B1 were significantly up-regulated in the cisplatin 10 mg·kg,-1,·d,-1, group(,P,<,0.05,P,<,0.01) .⑥Compared with the control group, cisplatin at dose of 10 mg·kg,-1,·d,-1, significantly inhibited the mRNA and protein expressions of StAR, CYP11A1, CYP17A1 and HSD3B2 in testis(,P,<,0.05,P,<,0.01), cisplatin at dose of 5 mg·kg,-1,·d,-1, significantly inhibited the mRNA expressions of ,Star, and ,Hsd3b2,(,P,<,0.05,P,<,0.01), and cisplatin at dose of 3 mg·kg,-1,·d,-1, significantly inhibited the mRNA expressions of ,Cyp17a1, and ,Hsd3b2,(,P,<,0.01) .,Conclusion:,2,Short-term administration of cisplatin can induce deficiency syndrome in mice, which mainly shows the deficiency of essence and qi, and its material basis is related to the decrease of steroid hormone synthesis and reserve.