最新刊期
期 S1 ， 2022
- 摘要：Objective： To observe the therapeutic effect and health economics evaluation of patients with acute cerebral infarction under the intervention mode of management pattern of stroke unit with integrated traditional Chinese and western medicine.MethodsNinety patients with acute cerebral infarction were randomly divided into treatment group and control group， 45 cases in each group. The treatment group was given the combined Chinese and western medicine stroke unit management model for intervention， and the control group was given the western medicine stroke unit management model. The treatment course of both groups was 2 weeks， followed up for 6 months. NIHSS score， Barthel index and related medical expenses were observed and compared.Results①There were statistically significant differences in NIHSS score and Barthel index between the two groups before treatment， 2 weeks after treatment and 6 months after treatment （P<0.05）. The improvement of NIHSS score and Barthel index in the treatment group was better than that in the control group at 2 weeks and 6 months after treatment. The improvement of NIHSS score and Barthel index in the treatment group was better than that in the control group at 2 weeks and 6 months after treatment （P<0.05）. ②The hospitalization cost in the treatment group was higher than that in the control group （P<0.05）， but the total medical cost within 6 months in the treatment group was lower than that in the control group （P<0.05）.ConclusionThe management pattern of stroke unit with integrated traditional Chinese and western medicine can significantly improve the neurological deficit and daily living ability of patients with acute cerebral infarction， and reduce related medical expenses. It has good clinical efficacy and economic benefit.关键词：management pattern of stroke unit with integrated traditional Chinese and western medicine;acute cerebral infarction;prognosis;health economics evaluation
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发布时间：2022-08-29 - 摘要：Objective： To evaluate the clinical efficacy of acupoint injection of astragalus injection combined with seretide on chronic obstructive pulmonary disease （COPD） at stable stage.MethodsNinety-five patients with stable COPD were enrolled in a randomized double-blind controlled study， and randomly divided into the treatment group （n=48） and the control group （n=47）. The patients in the treatment group were given acupoint injection of astragalus injection combined with seretide， and the patients in the control group were given normal saline injection combined with seretide for 3 months. The clinical symptoms and the occurrence of adverse reactions during treatment were recorded weekly with COPD clinical symptom grading quantification table. EpiData3.0 software was used for data management， and SPSS 26.0 statistical software was used for data analysis.ResultsThere was statistically significant difference between the two groups in the clinical efficacy of patients with stable COPD （P<0.05）. And there was a linear correlation between the clinical efficacy and treatment time （P<0.05）. Group was the main factor affecting the clinical efficacy evaluation of the two groups， and the clinical efficacy of the treatment group was better than that of the control group.ConclusionThe clinical efficacy of astragalus injection combined with seretide in the treatment of stable COPD is better than that of seretide group， and the clinical efficacy increases with the extension of treatment time. This treatment method is safe and effective. In clinic， patients can benefit more by appropriately prolonging the treatment course.关键词：chronic obstructive pulmonary disease;stable stage;course of treatment;repeated measurement design;curative effect evaluation
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of umbilical compress therapy of modified Banxia Xiexin Decoction combined with conventional western medicine in the treatment of acute duodenal ulcer bleeding.MethodsNinety patients of acute duodenal ulcer bleeding were randomly divided into the control group and treatment group， 45 cases in each group. The patients in the control group were treated with conventional western medicine， and the patients in the treatment group were treated with umbilical compress therapy of modified Banxia Xiexin Decoction based on the treatment for the control group， with a course of 5 days. The clinical efficacy was observed， and the changes of turning negative time of fecal occult blood， fasting time， hospital stay， traditional Chinese medicine syndrome score， hemoglobin level and blood urea nitrogen level were compared.Results①The apparent effective rate， effective rate and improvement rate of total syndrome score in the treatment group were higher than those in the control group （P<0.05）. ②The turning negative time of fecal occult blood in the treatment group was earlier than that in the control group， and the fasting time was shorter than that in the control group （P<0.05）. There was no statistically significant difference in the hospital stay between the two groups （P>0.05）. ③ After treatment， the levels of hemoglobin and urea nitrogen in both groups were improved （P<0.05）， and the improvement in the treatment group was better than that in the control group （P<0.05）.ConclusionCompared with conventional western medicine alone， umbilical compress therapy of modified Banxia Xiexin Decoction combined with conventional western medicine shows good efficacy in the treatment of acute duodenal ulcer bleeding， which can be widely used in clinic.关键词：duodenal ulcer bleeding;acute;Banxia Xiexin Decoction;umbilical compress therapy
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发布时间：2022-08-29 - 摘要：Objective： To observe the effect of Dahuang Fuzi Decoction on gastrointestinal function， inflammatory factors and oxidative stress injury in the patients with acute gastrointestinal injury （AGI） caused by sepsis.MethodsSeventy AGI patients caused by sepsis were randomly divided into treatment group and control group， 35 cases in each group. Both groups were treated with routine treatment，and the treatment group was additionally treated with Dahuang Fuzi Decoction， the treatment course was 14 days. After the treatment， the clinical efficacy of both groups was evaluated，and the changes in TCM syndrome score， gastrointestinal function indexe， inflammatory indexe， oxidative stress indexe were observed.Results①After treatment， the total effective rate was 91.4% in the treatment group and 77.1% in the control group， the difference in clinical efficacy between the two groups was statistically significant （P<0.05）. ②After treatment，the TCM symptom scores in two groups were both improved compared with those before treatment （P<0.05）， and the scores in the treatment group are lower than those in the control group （P<0.05）. ③After treatment， intra-abdominal pressure and defecation frequency in the two groups were improved compared with those before treatment （P<0.05），and the treatment group was superior than the control group （P<0.05）. ④After treatment， the AGI grade and APACHEⅡ score of the two groups were improved （P<0.05），and the treatment group was superior than the control group. （P<0.05）. ⑤After treatment，the levels of ROS and MDA in the two groups were decreased compared with those before treatment （P<0.05），and the treatment group decreased more significantly than the control group（P<0.05）. After treatment， the level of SOD in the two groups was increased compared with that before treatment，and the treatment group increased more significantly than the control group（P<0.05）. After treatment， the levels of TNF-α and IL-6 in the two groups were decreased compared with those before treatment（P<0.05）， and the treatment group decreased more significantly than the control group（P<0.05）.ConclusionDahuang Fuzi Decoction can restore the gastrointestinal function of AGI patients caused by sepsis，reduce inflammatory reaction and oxidative stress injury，alleviate clinical symptoms and improve the clinical curative effect of traditional Chinese medicine.关键词：sepsis;acute gastrointestinal injury;Dahuang Fuzi Decoction;oxidative stress injury;inflammatory factors;clinical study
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of Dachengqi Decoction on sepsis with acute gastrointestinal injury.MethodsForty patients suffering from sepsis with acute gastrointestinal injury were randomly divided into the treatment group and control group， 20 cases in each group. The control group was given routine treatment， and the treatment group was treated with Dachengqi Decoction on the basis of routine treatment. The treatment course of both groups was 7 days. The SOFA score， APACHE Ⅱ score， AGI score， intra-abdominal pressure， bowel sounds， and PCT， IL-6， TNF-α and IL-10 levels in serum were observed and compared.Results①The SOFA score and APACHE Ⅱ score in the two groups decreased significantly after treatment （P<0.05）. And those scores in the treatment group were lower than those in the control group （P<0.05）. ②After treatment， the intra-abdominal pressure and AGI scores in both groups were significantly decreased （P<0.05）， and the bowel sounds were significantly increased （P<0.05）. The intra-abdominal pressure and AGI scores in the treatment group were lower than those in the control group， and the bowel sounds in the treatment group were significantly more than those in the control group （P<0.05）. ③After treatment， the levels of PCT， IL-6 and TNF-α in serum in both groups decreased significantly （P<0.05） ， and the level of IL-10 increased significantly （P<0.05）. The levels of PCT， IL-6 and TNF-α in the treatment group were significantly lower than those in the control group （P<0.05）， and the levels of IL-10 in the treatment group were significantly higher than those in the control group （P<0.05）.ConclusionDachengqi Decoction combined with western medicine in the treatment of sepsis with acute gastrointestinal injury can effectively promote the recovery of gastrointestinal function and inhibit inflammatory reaction.关键词：Dachengqi Decoction;sepsis;acute gastrointestinal injury;clinical study
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of Qingyi Decoction as supplementary therapy on sepsis-induced acute liver injury （syndrome of bowel excess and heat bind）.MethodsSixty-four patients with sepsis-induced acute liver injury （syndrome of bowel excess and heat bind） were randomly divided into the control group and the treatment group， 32 cases in each group. The course of treatment was 7 days. Three days before and after treatment， and 7 days after treatment， all patients' liver function and severity index were observed. And the efficacy was evaluate.ResultsThe liver function and severity index of two groups were all improved after treatment （P<0.05）. Compared with the control group， the total bilirubin （TBIL） level of patients in the treatment group decreased faster in the early stage （P<0.05）， and the SOFA score of patients in the treatment group improved more significantly after the treatment （P<0.01）.ConclusionIt is preliminarily confirmed that the efficacy of Qingyi Decoction as supplementary therapy on sepsis-induced acute liver injury. In addition， it may improve the systemic inflammatory response of sepsis.关键词：sepsis-induced acute liver injury;syndrome of bowel excess and heat bind;Qingyi Decoction;liver immune
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发布时间：2022-08-29 - 摘要：Objective： To investigate the midnight snack in patients with non-alcoholic fatty liver disease （NAFLD） based on cross-sectional survey.MethodsTaking the physical examination population from Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine as the subjects， the body weight， BMI， blood glucose， blood lipid， liver function， uric acid and other related indexes of body metabolism， as well as the frequency， amount and type of midnight snack in NAFLD group and non-NAFLD group were investigated by sampling.Results①The body weight， waist circumference and BMI of NAFLD group were significantly higher than those of non-NAFLD group （P<0.05）. ②Compared with non-NAFLD group， the serum levels of ALT， AST， γ-GT， FBG， and LDL in the NAFLD group showed increase tendency （P<0.05）. ③The serum contents of TG， TC and UA in NAFLD group were significantly higher than those in non-NAFLD group （P<0.05）. ④The proportion of people with midnight snack in NAFLD group was significantly higher than that in non-NAFLD group （χ²=10.058， P=0.002）. ⑤There was statistically significant difference on the frequency of midnight snack every week between NAFLD group and non-NAFLD group （χ²=6.112， P=0.013）. ⑥For the types of midnight snack， seafood barbecue， dessert and sugary drinks were often found in NAFLD group， while fruits and vegetables， milk， dairy products and nuts were observed in non-NAFLD group.ConclusionThe proportion and the times per week of midnight snack in NAFLD group are higher than those in non-NAFLD group， and the type of midnight snack is different between the two groups. Midnight snack may be an important risk factor for NAFLD.关键词：non-alcoholic fatty liver disease;midnight snack;cross-sectional survey
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发布时间：2022-08-29 - 摘要：Objective： To observe the efficacy of Jijing Quanxing Formula in the treatment of metabolism-related nephropathy （CKD at stage 1-4）.MethodsIn the treatment group， 70 patients of metabolic related nephropathy with syndrome of kidney deficiency and blood stasis （CKD at stage 1-4） were included and treated with Jijing Quanxing Formula. According to the ratio of 2∶1， 35 patients with similar condition in the same period， similar age and consistent disease distribution were selected as the control group. The changes of the renal related indexes and the effective rates were compared within and between groups.ResultsAfter treatment， the relevant laboratory indexes were improved in both groups， and the improvement of the treatment group was better than that of the treatment group （P<0.05）.ConclusionThe improvements of Jijing Quanxing Recipe on the renal function and the decrease of urinary protein are more obvious in patients with diabetic nephropathy， hypertensive nephropathy， CKD at stage 3 and 4.关键词：Jijing Quanxing Formula;metabolic related nephropathy;CKD at stage 1-4;accumulating essence for morphological recovery;treating different diseases with same method;clinical observation
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发布时间：2022-08-29 - 摘要：Objective： To observe the efficacy of Miniao Paishi Mixture on removing residual stones after extracorporeal shock wave lithotripsy （ESWL） of renal calculi.MethodsNinety patients with renal calculi were randomly divided into the control group and observation group， 45 cases in each group. Both groups were treated with ESWL on the day of admission. After operation， the patients drank 2 500-3 000 ml of water every day and do jumping exercise appropriately. The control group was treated with tamsulosin hydrochloride sustained-release capsule on this basis， and the observation group was treated with Miniao Paishi Mixture based on the treatment for the control group， with a course of 4 weeks. The clinical efficacy and the stone excretion were observed， the changes of clinical symptom scores and related indexes of renal function were compared， and the safety was evaluated.Results①The clinical efficacy of the observation group was better than that of the control group （P<0.05）. ② After treatment， the clinical symptom scores of the two groups were decreased （P<0.05）， and the clinical symptom scores of the observation group were less than those of the control group （P<0.05）. ③The removal rate of residual stone in the observation group was higher than that in the control group （P<0.05）， the stone removal time and disappear time in the observation group were less than those in the control group （P<0.05）， and the recurrence rate of the observation group was lower than that of the control group （P<0.05）. ④After treatment， the levels of serum creatinine and urea nitrogen in the two groups were decreased （P<0.05）， and the levels of serum creatinine and urea nitrogen in the observation group were lower than those in the control group （P<0.05）. ⑤There were no obvious adverse reaction in both groups.ConclusionMiniao Paishi Mixture can effectively improve the stone clearance rate after ESWL， alleviate local clinical symptoms， improve patients’ renal function， and has high safety.关键词：renal calculi;extracorporeal shock wave lithotripsy;Miniao Paishi Mixture;renal function
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发布时间：2022-08-29 - 摘要：Objective： To explore the clinical efficacy of Fuyuan Tongluo Formula in the treatment of stent implantation and restenosis of diabetic lower limb vascular disease.MethodsSixty patients with stent implantation and restenosis of diabetic lower limb vascular disease were randomly divided into traditional Chinese medicine group and control group， 30 cases in each group. Both groups were treated with routine western medicine such as hypoglycemic and anti-hypertensive drugs. On this basis， the traditional Chinese medicine group was treated with Fuyuan Tongluo Formula for 6 treatment courses （4 weeks as one treatment course）. The total clinical effective rate， 6-minute walking and claudication distance， ABI， TcPO2， lower extremity artery diameter and safety indexes were compared between the two groups.Results①After treatment for 6 months， the total effective rate of the traditional Chinese medicine group was 90.0%， and that of the control group was 80.0%. The efficacy of the traditional Chinese medicine group was better than that of the control group （P<0.05）. ②Three and six months after operation， the 6-minute walking distance in the traditional Chinese medicine group was significantly longer than that in the control group （P<0.05）. Six months after operation， the 6-minute claudication distance in the traditional Chinese medicine group was significantly longer than that in the control group （P<0.05）. ③The resting ABI and TcPO2 in the traditional Chinese medicine group were significantly higher than those in the control group （P<0.05）. ④Three and six months after operation， the diameters of femoral artery， popliteal artery and posterior tibial artery in the traditional Chinese medicine group were wider than those in the control group （P<0.05）.ConclusionFuyuan Tongluo Formula can improve the clinical symptoms of stent implantation and restenosis of diabetic lower limb vascular disease， promote the revascularization of lower extremities， and delay the occurrence of restenosis.关键词：Fuyuan Tongluo Formula;diabetic lower limb vascular disease;stent implantation;restenosis;clinical efficacy
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发布时间：2022-08-29 - 摘要：Objective： To study the correlation between oxidative stress， inflammation related indexes and diabetic foot ulcers.MethodsThe clinical data of 120 patients with type 2 diabetes mellitus were retrospectively analyzed. According to whether complicated with diabetic foot， the patients were divided into the simple group and combined group， 60 cases in each group. Another 60 healthy subjects in the same period were taken as the control group. The levels of serum superoxide dismutase （SOD）， serum amylase A （SAA）， high sensitivity C-reactive protein （hs-CRP）， procalcitonin （PCT）， D-dimer， coagulation factor Ⅷ and neutrophil count were detected by enzyme-linked immunosorbent assay （ELISA）， and the level of serum glycosylated hemoglobin （HbA1c） was detected by automatic biochemical analyzer. The differences of oxidative stress and inflammation related indexes in each group were analyzed and compared. The risk factors of diabetic foot ulcers were studied by Logistic regression analysis.Results①The prevalence of hypertension and coronary heart disease and the serum levels of HbA1c， hs-CRP and PCT in the combined group were higher than those in the control group （P< 0.001）. The serum levels of D-dimer， coagulation factor Ⅷ， SAA and neutrophil count were higher than those in the control group and simple group， and the level of serum SOD was lower than those in the control group and simple group （P<0.001）. ②Serum SOD level was negatively correlated with diabetic foot ulcers， and serum SAA level was positively correlated with diabetic foot ulcers （r=0.778， P<0.001； r=-0.603， P<0.001）. ③Logistic regression analysis showed that SAA and SOD levels were independent risk factors for occurrence diabetic foot ulcers.ConclusionOxidative stress injury is serious in diabetic foot ulcers patients. Monitoring SOD and SAA levels is helpful to assess the degree of diabetic foot lesions.关键词：type 2 diabetes mellitus;diabetic foot;oxidative stress;inflammation;superoxide dismutase;serum amylase A
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of Modified Sijunzi Decoction in treating perennial allergic rhinitis with syndrome of deficiency of lung and spleen qi.MethodsOne hundred and eighteen cases of perennial allergic rhinitis with syndrome of deficiency of lung and spleen qi were included as subjects， and were randomly divided into the treatment group and control group， 59 cases in each group. The treatment course was 4 weeks in both groups. The clinical efficacy， VAS score， quadrant score， nasal endoscopy score and TCM syndrome score were compared between the two groups.Results①During the treatment， 2 cases fell off in each group， and no adverse reactions were found in all cases. ②After treatment for 4 weeks， the total effective rates of the treatment group and the control group were 91.23% and 75.44% respectively， with statistically significant difference （P<0.05）. ③The VAS score， quartile score， size of inferior nasal concha and nasal mucosa color score in the treatment group were lower than those in the control group. There were statistically significant differences between the two groups in the improvement of large amount of clear runny nose， shortness of breath， weakness， loose stool， cold prone， nasal congestion， runny nose symptoms and size of inferior nasal concha （P<0.05）.ConclusionCompared with cetirizine hydrochloride alone， Modified Sijunzi Decoction combined with cetirizine hydrochloride shows better improvements on nasal congestion， runny nose， size of inferior nasal concha， large amount of runny nose， shortness of breath and fatigue， loose stool and cold prone.关键词：Sijunzi Decoction;perennial allergic rhinitis;syndrome of deficiency of lung and spleen qi;allergic rhinitis
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发布时间：2022-08-29 - 摘要：Objective： To explore the clinical effect of Sanfeng Tongqiao Dripping Pills combined with mometasone furoate nasal spray in treatment of chronic simple rhinitis.Methods116 patients with chronic simple rhinitis were randomly divided into treatment group and control group，58 cases in each group. The control group was treated with mometasone furoate nasal spray，and the treatment group was additionally treated with Sanfeng Tongqiao Dripping Pills orally on the basis of the control group，the course of treatment was 4 weeks. VAS score of nasal obstruction and patients' subjective dependence on mometasone furoate nasal spray were recorded in the two groups before treatment， the day after treatment and week 12 after treatment.Results①Compare within groups， VAS scores of nasal obstruction in the two groups the day after treatment and week 12 after treatment were lower than those before treatment（P<0.05）； compared between two groups at week 12 after treatment， VAS score of nasal obstruction in the treatment group was lower than that in the control group（P<0.05）. ②At week 12 after treatment， the percentage of subjective dependence on mometasone furoate nasal spray in the treatment group and control group was 15.5%、70.7% respectively， and the difference was statistically significant （P<0.05）.ConclusionSanfeng Tongqiao Dripping Pills combined with mometasone furoate nasal spray in the treatment of chronic simple rhinitis can effiectively improve the symptoms of nasal obstruction and reduce patients’ subjective dependence on mometasone furoate nasal spray.关键词：chronic simple rhinitis;mometasone furoate nasal spray;Sanfeng Tongqiao Dripping Pills;visual analogue scale
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of Sangqin Mixture combined with nasal scratch surger in the treatment of rosacea with syndrome of wind-heat in lung meridian.MethodsNinety-five patients of rosacea with syndrome of wind-heat in lung meridian were randomly divided into the control group （46 cases） and treatment group （49 cases）. The control group was treated with routine nasal scratch surgery， and the treatment group was treated with Sangqin Mixture based on the treatment for the control group. The patients were followed-up at 3， 6 and 12 months after operation， the recovery of nasal shape in the two groups was observed， and the scores of nasal skin erythema， telangiectasia， oil secretion and nasal skin thickening were compared.Results①Six and twelve months after operation， there was no statistically significant difference in the overall nasal shape recovery between the two groups （P>0.05）. ②Compared with those before treatment， there were significant differences in the scores of nasal skin erythema， telangiectasia， oil secretion and skin thickening in the two groups at 3， 6 and 12 months after operation （P<0.05）. ③Three and six months after operation， there were no statistically significant difference in the scores of nasal skin erythema， telangiectasia and oil secretion between the two groups （P>0.05）. Twelve months after operation， the scores of nasal skin erythema and oil secretion in the treatment group were lower than those in the control group （P<0.05）.ConclusionSangqin Mixture combined with nasal scratch surgery shows good efficacy in the treatment of rosacea with syndrome of wind-heat in lung meridian， which can correct the nasal shape， alleviate the symptoms of rosacea， and exert more persistent effect on improving local erythema and skin oil secretion.关键词：rosacea;syndrome of wind-heat in lung meridian;Sangqin Mixture;scratch surgery
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发布时间：2022-08-29 - 摘要：Objective： To observe the effect of Zhu’s Bushen Huoxue Formula on pregnancy outcome and serum interleukin（IL）-2 ， IL-4 and IL-10 levels in recurrent spontaneous abortion （RSA） patients with kidney deficiency and blood stasis.MethodsTwenty patients with RSA and 20 patients with normal termination of pregnancy were selected， and the serum levels of IL-2， IL-4 and IL-10 in the two groups were detected by ELISA. Sixty-four patients with kidney deficiency and blood stasis RSA were randomly divided into two groups， 32 cases in the treatment group and 32 cases in the control group. The control group was given dydrogesterone orally for routine fetal protection， while the treatment group was given traditional Chinese medicine（TCM） Bushen Huoxue Formula orally on the basis of the control group. The changes of TCM syndrome scores， serum levels of IL-2， IL-4， IL-10 and pregnancy outcomes were compared between the two groups before and after treatment.Results①The levels of IL-2 and IL-4 in peripheral blood of RSA patients were significantly higher than those of normal group， and IL-10 was significantly lower than that of normal group （P<0.01）. ②After treatment， TCM syndrome score and serum IL-2 and IL-4 levels decreased in both groups， while serum IL-10 level increased. The results in the treatment group were more obvious than those in the control group， and the live birth rate in the treatment group was higher than that in the control group （P<0.01）.ConclusionThe serum levels of IL-2， IL-4 and IL-10 in patients with RSA were significantly higher than those in the normal group， while the level of IL-10 were significantly lower than those in the normal group（P<0.01）， suggesting that inflammatory factors IL-2， IL-4 and IL-10 may be involved in the pathogenesis of RSA. Zhu's Bushen Huoxue Formula may improve the live birth rate and pregnancy outcome of RSA patients by reducing the levels of IL-2， IL-4 and increasing the level of IL-10 in peripheral blood.关键词：recurrent spontaneous abortion;Bushen Huoxue Formula;pregnancy outcome;interleukin
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of Ningshen Ankun Formula in the treatment of climacteric syndrome.MethodsEighty patients with climacteric syndrome were randomly divided into the control group and observation group， 40 cases in each group. The control group was treated with Kunbao Pill， and the observation group was treated with Ningshen Ankun Formula， with a course of 1 month. The clinical efficacy was observed， the changes of Kuppenrman score， menopausal quality of life （MENQOL） scale， sex hormone levels and related laboratory indexes were compared， and the safety was evaluated.Results①The clinical efficacy of the observation group was better than that of the control group （P<0.05）. ②The improvement of Kuppenrman score， MENQOL score， sex hormones and related laboratory indexes in the observation group were better than those in the control group （P<0.05）. ③There were no significant adverse reaction in both groups.ConclusionCompared with Kunbao Pill， Ningshen Ankun Formula can better improve the clinical symptoms of patients with climacteric syndrome， regulate the levels of luteinizing hormone， estradiol and follicle stimulating hormone， and has good safety.关键词：climacteric syndrome;premenopausal and postmenopausal syndromes;Chinese medical therapy
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发布时间：2022-08-29 - 摘要：Objective： To evaluate the clinical curative effect of Huoxiang Zhengqi Powder combined with conventional western medicine on acute norovirus gastroenteritis of cold-damp type and its influence on serum inflammatory factors.MethodSixty-four children with acute norovirus gastroenteritis of cold-damp type were selected and randomly divided into observation group and control group， 32 cases in each group. The control group was treated with conventional western medicine， and the observation group was treated with Huoxiang Zhengqi Powder on the basis of control group. The treatment course of both groups was 5 days. The clinical efficacy and the disappearance time of principal symptoms were compared between the two groups. Before treatment and after treatment， traditional Chinese medicine syndrome score， interleukin（IL）-6 and IL-1β in serum were compared between the two groups.Results①The total clinical efficacy rates of observation group and control group were 93.55% and 73.33%， respectively， and the curative effect of observation group was better than control group （P<0.05）. ②During the treatment， the disappearance time of diarrhea， nausea and vomiting in the observation group were significantly shorter than those of the control group （P<0.05）. ③After treatment， the Chinese medicine syndrome score of both groups was lower than that before treatment （P<0.05）， and the score in the observation group was lower than that in the control group （P<0.05）. The serum levels of IL-6， IL-1β in the two groups were lower than those before treatment， and the serum level of IL-1β in observation group was lower than that in the control group （P<0.05）.ConclusionHuoxiang Zhengqi Powder combined with conventional western medicine can effectively improve the clinical symptoms of children with acute norovirus gastroenteritis of cold-damp type， and reduce the levels of serum inflammatory factors.关键词：norovirus;acute infectious gastroenteritis;cold-damp type;Huoxiang Zhengqi Powder;combination of Chinese and western medicine
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发布时间：2022-08-29 - 摘要：Objective： To evaluate the treatment effect of endoscopic application of tissue adhesive sandwich injection in treatment of Dieulafoy’s disease bleeding.MethodsA total of 61 patients with massive bleeding due to Dieulafoy’s disease were randomly divided into group A and group B， with 31 cases in group A and 27 cases in group B. Group A was given endoscopic cyanoacrylate sandwich injection for hemostasis， and group B was given endoscopic high-frequency electrocoagulation hemostatic forceps for hemostasis. After treatment， the success rate of hemostasis and the rate of rebleeding within 72 hours after operation were compared between the two groups. At the same time，the postoperative complications such as ectopic embolism and tissue necrosis were observed.ResultsThere was no statistical difference in the hemostatic rate between the two groups（P>0.05），but the 72-hour rebleeding rate in group B was significantly higher than that in group A， and the difference was statistically significant （P<0.05）.ConclusionsTissue adhesive sandwich injection is a fast， effective and safe treatment for gastroscopic hemostasis in Dieulafoy’s disease.关键词：Dieulafoy disease;emergency treatment;hemostasis;tissue adhesive;clinical observation
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of traditional Chinese medicine combined with acupuncture and conventional western medicine treatment in the treatment of chronic heart failure （cardiac function grade Ⅱ） with Qi deficiency and blood stasis syndrome.MethodsSeventy-two patients with chronic heart failure （cardiac function grade Ⅱ） were randomly divided into treatment group and control group， 36 cases in each group. The control group was treated with basic western medicine. On the basis of the control group， the treatment group was treated with traditional Chinese medicine combined with acupuncture. The treatment course of both groups was 4 weeks. The clinical efficacy was observed. Before and after treatment， the left ventricular ejection fraction （LVEF） was calculated， and the serum levels of N-terminal pro-brain natriuretic peptide （NT- ProBNP）， transforming growth factor-β1 （TGF-β1）， connective tissue growth factor （CTGF）， procollagen Ⅰ （PC Ⅰ） and procollagen Ⅲ （PC Ⅲ） in patients of both groups were detected and compared.ResultsDuring the experiment， 5 patients in the treatment group and 6 patients in the control group were excluded or dropped out. Finally， 31 patients in the treatment group and 30 patients in the control group were included in the statistical analysis. ①After treatment, The total clinical effective rate of the treatment group and the control group was 74.19% and 63.33%， respectively. And the clinical curative effect of the treatment group was significantly better than that in the control group （P<0.05）. ②After the treatment， the LVEF level in both groups was significantly increased （P<0.05）. The serum levels of NT-ProBNP， TGF-β1， CTGF， PCⅠ， PC Ⅲ were significantly decreased， and the level of LVEF in the treatment group was higher than that in the control group （P<0.05）.ConclusionCompared with the conventional treatment of western medicine， traditional Chinese medicine combined with acupuncture and conventional western medicine treatment can effectively improve the index of heart failure in patients suffering from chronic heart failure （cardiac function grade Ⅱ） with Qi deficiency and blood stasis syndrome and improve the clinical efficacy.关键词：chronic heart failure;Qi deficiency and blood stasis syndrome;acupuncture;traditional Chinese medicine
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of treating diabetes complicated with insomnia by embedding needle at Shu-acupoint.MethodsEighty patients with diabetes complicated with insomnia were randomly divided into treatment group and control group， 40 cases in each group. The control groups were given basic treatments （hypoglycemic drugs and health education） combined with alprazolam tablets. The treatment group was given basic treatments combined with embedding needle at Shu-acupoint. The clinical efficacy was observed and the changes of fasting blood glucose （FPG）， 2 h postprandial blood glucose （2 hPG） and Pittsburgh Sleep Quality Index （PSQI） score were compared.Results①The total effective rates of treatment group and control group were 87.50% and 60.00%， respectively. Compare the clinical efficacy of insomnia between the two groups， the treatment group was significantly superior to the control group （P<0.05）. ②There were statistically significant differences in FPG and 2hPG levels between the two groups before and after treatment （P<0.05）. After treatment， the FPG and 2 hPG levels in treatment group was significantly lower than those in the control group （P<0.05）. ③There were statistically significant differences in PSQI score between the two groups before and after treatment （P<0.05）. After treatment， the PSQI score in treatment group was significantly lower than that in the control group （P<0.05）.ConclusionTreating diabetes complicated with insomnia by embedding needle at Shu-acupoint can improve the sleep disorders and glucose metabolism disorders of the patients.关键词：diabetes;insomnia;embedding needle at Shu-acupoint;Pittsburgh sleep quality index
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发布时间：2022-08-29 - 摘要：Objective： To evaluate the improvement of electroacupuncture （EA） on immunosuppression in breast cancer patients after surgery.MethodsSixty female patients underwent modified radical mastectomy for breast cancer were selected and randomly divided into control group （CON group） and electroacupuncture group （EA group）， 30 cases in both groups. Patients in the EA group were given EA intervention before， during and after surgery， while patients in the CON group were not intervened. The main observation indicators are the changes in the percentage of CD4+T， CD8+T， CD4+/CD8+ ratio and NK cells in breast cancer patients before surgery， day 1 and day 3 after surgery.ResultsCompared with the CON group， immune-related cells （CD4+T， CD8+T， CD4+/CD8+ and NK cells） in the EA group has no statistically significant difference before surgery and day 1 after surgery （P>0.05）. Day 3 after surgery， there was statistically significant difference in immune-related cells （CD4+T， CD4+/CD8+） between the EA group and the CON group （P<0.05）.ConclusionEA can improve the postoperative immunosuppression of breast cancer patients by enhancing the body's adaptive immune function.关键词：electroacupuncture;breast cancer;immunosuppression
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发布时间：2022-08-29 - 摘要：ObjectiveTo observe the clinical effect of acupuncture combined with traditional Chinese medicine （TCM） on polycystic ovary syndrome patients with kidney Yang deficiency syndrome.MethodsNinety-two polycystic ovary syndrome patients with kidney Yang deficiency syndrome were randomly divided into treatment group and control group， 46 cases in each group. All patients were given TCM orally， acupuncture was added in the treatment group， and the placebo acupuncture was added to the treatment group in the control group. The treatment course of both groups was 3 menstrual cycles. The curative effect and safety of TCM syndrome were observed， and the menstrual cycle， anti-Mullerian hormone （AMH）， luteinizing hormone （LH）， follicle stimulating hormone （FSH）， testosterone （T） levels and ovulation rate were compared.Results①Before treatment， there was no statistical significance in menstrual cycle between two groups （P>0.05）. After treatment， the menstrual cycle was significantly shortened in both groups （P<0.05）， and the improvement in the treatment group was better than that in the control group （P<0.05）. ②The total effective rate of TCM syndrome in treatment group and control group were 91.30% and 76.09%， respectively. There was statistically significant difference in TCM syndrome curative effect between two groups， and the curative effect of the treatment group was better than the control group （P<0.05）. ③In intra-group comparison before and after treatment， there were statistically significant differences in FSH， LH and T levels in the treatment group， while there were statistically significant differences in LH and T levels in the control group （P<0.05）. After treatment， the level of LH in the treatment group was significantly lower than that in the control group. ④The ovulation rates of treatment group and control group were 67.39% and 36.96% respectively， ovulation rate in treatment group was significantly higher than that in control group （P<0.05）. ⑤During the experiment， there were no adverse event in the two groups.ConclusionThe clinical effect of acupuncture combined with TCM on polycystic ovary syndrome patients with kidney Yang deficiency syndrome. The combination of acupuncture and TCM in the treatment of polycystic ovary syndrome patients with kidney Yang deficiency syndrome has a significant effect， which can effectively improve the TCM syndrome and menstrual cycle， regulate the level of sex hormones， improve ovulation rate．关键词：acupuncture;combination of acupuncture and medicine;polycystic ovary syndrome;kidney Yang deficiency syndrome;clinical observation
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发布时间：2022-08-29 - 摘要：ObjectiveTo study the effect of transcutaneous electrical acupoint stimulation （TEAS） combined with palonosetron on postoperative nausea and vomiting （PONV） in patients after hysteroscopic surgery.MethodsEighty patients undergoing hysteroscopic surgery were selected and randomly divided into Palonosetron group （group A） and TEAS combined with Palonosetron group （group B）， 40 cases in each group. The occurrence of nausea and vomiting at 1 h， 6 h， 12 h and 24 h after surgery was recorded. The time of the first anal exhaust after operation and the patients’ satisfaction score at 24 h after surgery was evaluated.Results①Compared with group A， the incidence of PONV at 1 h and 6 h after operation in group B was significantly decreased （P<0.05）. There was no significant difference in the incidence of PONV between the two groups at 12 h and 24 h after surgery （P>0.05）. ②Compared with group A， the first anal exhaust time after operation was significantly shorter in group B （P<0.05）. ③Compared with group A， postoperative satisfaction score of patients was significantly higher in group B （P<0.05）.ConclusionTEAS combined with intravenous palonosetron can significantly reduce the incidence of nausea and vomiting in patients after hysteroscopic surgery， shorten the first anal exhaust time after surgery and improve patients’ satisfaction， which is beneficial to the quick recovery of patients after surgery.关键词：transcutaneous electrical acupoint stimulation;palonosetron;postoperation;nausea and vomiting;hysteroscope
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发布时间：2022-08-29 - 摘要：Objective： To observe the clinical efficacy of electroacupuncture combined with pudendal nerve block in anal fistula operation.MethodsOne hundred and twenty patients undergoing anal fistula operation were taken as subjects， and were randomly divided into the experimental group （electroacupuncture at Baliao acupoint combined with pudendal nerve block） and the control group （spinal anesthesia）， 60 cases in each group. The changes of mean arterial pressure （MAP） and heart rate （HR） before and after operation， the time of operation， the time of the first postoperative pain， the grade of postoperative pain and the postoperative complications were compared between the two groups， and the safety was evaluated at the same time.Results①There was no significant difference in preoperative and intraoperative mean arterial pressure and heart rate in the experimental group （P>0.05）， and the intraoperative mean arterial pressure and heart rate in the control group were lower than those before operation （P<0.05）. ②There was no significant difference in operation time between the two groups （P>0.05）. The occurrence time of the first postoperative pain in the experimental group was significantly prolonged compared with that in the control group （P<0.05）. ③At 6 h， 12 h and 24 h after operation， the degree of pain in the experimental group was lower than that in the control group. ④The incidence rates of postoperative urinary retention and nausea and vomiting in the experimental group were significantly lower than those in the control group.ConclusionThe anesthetic method of electroacupuncture at Baliao acupoint combined with pudendal nerve block can meet the needs of analgesia and anal relaxation required by operation， with no circulatory fluctuation， but longer analgesia time and better motor function， which could promote the enhanced recovery after surgery （ERAS）.关键词：electroacupuncture;Baliao acupoint;pudendal nerve block;anal fistula;anorectal surgery;anesthesia;spinal anesthesia
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发布时间：2022-08-29 - 摘要：Objective： To observe the influence of holistic nursing in operating room on elderly patients’ psychological state and life quality after laparoscopic surgery.MethodsNinety-six elderly patients after laparoscopic surgery were randomly divided into control group and observation group， 48 cases in each group. During the treatment， patients in the control group were treated with routine nursing， and patients in the observation group were treated with holistic nursing in the operating room. The changes of Zung anxiety Scale （SAS）， Depression Scale （SDS） and Brief Health Questionnaire （SF-36） scores were observed and compared before and after nursing.Results①After nursing， the scores of SAS and SDS in the two groups were significantly deceased （P<0.05）. And the SAS and SDS scores in the observation group were significantly lower than those in the control group （P<0.05）. ②After nursing， the SF-36 score was significantly increased in both groups （P<0.05）. And the SF-36 scores in the control group was significantly lower than that in the observation group （P<0.05）.ConclusionDuring the nursing of elderly patients undergoing laparoscopic surgery， holistic nursing in the operating room can significantly improve the negative emotions of patients and improve the patients’ life quality.关键词：holistic nursing in operating room;laparoscopic surgery for elderly patient;psychological state;life quality
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发布时间：2022-08-29
Clinical Research
- 摘要：To summarize the theoretical basis for the treatment of chronic obstructive pulmonary disease（COPD） complicated with gastrointestinal dysfunction by “regulating Qi activity， reducing phlegm， and facilitating bowel movement”， and the basis and advantages of the application of Chailing Jiangqi Decoction， also called Dachaihu Decoction with Linggan Wuwei Jiangxin Xiaxing Decoction. It is believed that chronic obstructive pulmonary disease is a common， preventable and treatable disease that can seriously harm human’s health. In addition to the symptoms of choking lung gas such as cough， sputum， wheezing and fullness， its clinical manifestations are complicated with gastrointestinal dysfunction such as abdominal distension and constipation. COPD is the syndrome of primary deficiency， and various pathologic products can lead to pulmonary and intestinal Qi obstructing over time， causing acute attack or gastrointestinal dysfunction. “Qi movement stagnation” is an important mechanism of acute exacerbatio chronic obstructive pulmonary disease（AECOPD ）complicated with gastrointestinal dysfunction.关键词：chronic obstructive pulmonary disease;gastrointestinal dysfunction;Dachaihu Decoction;Linggan Wuwei Jiangxin Xiaxing Decoction;traditional Chinese medicine therapy
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发布时间：2022-08-29 - 摘要：This paper discusses professor Yang xuejun’s experience in treating urinary tract infections in middle-aged and elderly women. It is believed that the majority of its incidence population is middle-aged and elderly women， the etiology mechanism is the heart and kidney incompatibility， and the humid and hot bets are the standard， the treatment takes the heart as the entry point， calms the spirit， clears the damp heat， and the traffic heart and kidney. And cite 1 case.关键词：urinary tract infection;from the heart to treat;clinical experience;tradional Chinese medicne therapy
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发布时间：2022-08-29 - 摘要：To explore Professor Yin Xiuqi’s experience in syndrome differentiation and treatment of polycystic ovary syndrome. Consider that the clinical manifestation of some polycystic ovarian syndrome cases can be attributed to pulmonary functional disorder， and attention should be paid to the pathogenesis of lung in the pathogenesis of polycystic ovary syndrome. And ventilating the lung， keeping the qi flowing downward and replenishing yin to relieve dryness of the lung could be added in therapy. Commonly used drugs include Fritillariae Cirrhosae Bulbus， Platycodonis Radix， Trichosanthis Pericarpium， Mori Cortex， Chloriti Lapis， Pinelliae Rhizoma， Menthae Haplocalycis Herba， Cicadae Periostracum， etc.关键词：polycystic ovary syndrome;lung;Xuan Fei Su Jiang;traditional Chinese medicine therapy
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发布时间：2022-08-29 - 摘要：To explore the experience of treating infertility of polycystic ovary syndrome under the guidance of the theory of “water out of the kidneys”. Consider that polycystic ovary syndrome is one of the main causes of menstrual disorders and ovulation disorder infertility. In traditional Chinese medicine， it is believed that women “menstrual fetal birth” with menstruation first， and pregnancy must be after regulating menstruation. menstrual adjustment is the basis of fetal pregnancy， follow the “water out of the kidneys” theory， differentiate syndromes and treat olycystic ovary syndrome infertility by the kidney deficiency essence. And 1 case was cited.关键词：“menstruation comes from the kidney”;irregular menstruation;polycystic ovary syndrome;infertility;traditional Chinese medicine therapy
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发布时间：2022-08-29
Clinical Experience
- 摘要：Objective： To screen the key genes of Glioma Decoction-1 （GD-1） in combination with temozolomide （TMZ） against glioma by transcriptomics study.MethodsA subcutaneous glioma heterotopic model was established in mice， and after successful modeling， the mice were grouped for administration， and the tumor volume was measured every third day. Samples were taken after 14 days， and the pathological histomorphology of tumors was observed by HE staining. The potential mechanism of GD-1 combined with TMZ against glioma was predicted by transcriptomics and literature review.ResultsCompared with the control group， the average tumor volume of nude mice in the TMZ and TMZ-GD-1 groups was significantly reduced （P<0.05）， and compared with the TMZ group， the tumor volume of the TMZ-GD-1 group was reduced， but the difference was not statistically significant （P>0.05）.He staining showed the highest growth density of glioblastoma in the control group with the largest and hyperchromatic nuclei， and compared with the TMZ group， the TMZ-GD-1 group showed lower growth density of glioblastoma with lighter nuclei and more nuclei emerged pyknosis and lysis. By transcriptomics study， 65 differential genes were found in the GD-1 combined with TMZ group compared with the TMZ group， including 36 up-regulated genes and 29 down-regulated genes. Through GO enrichment analysis and KEGG enrichment analysis， the differential genes related to differentiation， apoptosis and biological signaling transmission during cell development were SLC17A7， CACNA1D， MTCP1， RASIP1， BTBD16， among which CACNA1D was down-regulated in MAPK signaling pathway， MTCP1 was down-regulated in PI3K/Akt signaling pathway， and CACNA1D was down-regulated in the cGMP/PKG signaling pathway.ConclusionGD-1 combined with TMZ has better effect in the treatment of glioma than the Western medicine TMZ alone， which could further reduce the tumor volume and lower tumor growth density. The mechanism may be up-regulation of inhibitory oncogenes （SLC17A7， RASIP1， BTBD16） and down-regulation of oncogenes （MTCP1， CACNA1D）， which further affects the activity of PI3K/Akt， cGMP/PKG， MAPK signaling pathways， thereby inhibiting glioma growth.关键词：Glioma Decoction 1;temozolomide;glioma;transcriptome sequencing;action mechanism
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发布时间：2022-08-29 - 摘要：Objective： To observe effect and mechanism of Huangci Granules on 5-fluorouracil （5-FU） induced cognitive impairment in mice.MethodsThe mice were randomly divided into control group， model group （5-FU， 60 mg/kg）， low-dose Huangci Granules group （1.8 g/kg） and high-dose Huangci Granules group （5.4 g/kg）. The changes of motor ability and memory ability were observed by open field test and new and old objects recognition test. The neurogenesis and gliosis in the brain of mice were investigated by immunohistochemistry in each group.Results①There was no significant difference in the motor ability of mice in each group. ②Compared to the control group， the memory ability of the model mice decreased significantly （P<0.05）， and Huangci Granules could significantly improve the impairment of memory ability in mice （P<0.05）. ③Compared to the control group， the expression of Doublecortin （DCX） in the subgranule zone of the dentate gyrus and subventricular zone of the lateral ventricle decreased significantly in mice of the model group （P<0.05）， and both low dose and high dose Huangci Granules could significantly increase the expression of DCX （P<0.05）. ④Compared to the control group， microglia and astrocytes in the hippocampus of the model group significantly proliferated and activated （P<0.05）， and Huangci Granules could significantly reduce the proliferation and activation of microglia and astrocytes in the hippocampus （P<0.05）.ConclusionHuangci Granules can alleviate 5-FU induced cognitive impairment in mice through improving nerve regeneration injury and gliosis.关键词：Huangci Granules;5-fluorouracil;nerve regeneration;gliosis;chemotherapy-related cognitive impairment
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发布时间：2022-08-29 - 摘要：Objective： To explore the protective effect and preliminary mechanism of Qilong Quyu Decoction on ischemic stroke.MethodsThe model of middle cerebral artery occlusion （MCAO） in mouse was established. The mice were randomly divided into sham operation group， model group， Qilong Quyu Decoction low-dose group （10 g/kg）， medium-dose group （20 g/kg） and high-dose group （30 g/kg）. After modeling， the mice were administered intragastrically， and the neurological function score was evaluated after 24 h. TTC staining was used to evaluate the volume of cerebral infarction in mice. The number of neurons and the activation of microglia and astrocyte in the cortex region were observed by immunohistochemistry. The expressions of SOD1， SOD2， CAT and GPX-4 were detected by Western blot； The epressions of IL-6， TNF-α and IL-1β were detected by ELISA kit.ResultsCompared with the model group， medium-dose and high-dose Qilong Quyu Decoction group improved the neurological injury， the volume of cerebral infarction and neurological deficit after MCAO in mice（P<0.05）. Meanwhile， high-dose Qilong Quyu Decoction significantly reduced the injury of infarcted cortical neurons and the activation of microglia and astrocyte， increased the protein levels of CAT and GPX-4， reduced the level of ROS expression in the brain， and decreased the inflammatory factor expressions of IL-6、TNF-α and IL-1β（P<0.05）.ConclusionQilong Quyu Decoction has a positive protective effect on cerebral ischemia-reperfusion injury in mice， and its mechanism may be closely related to its anti-inflammatory and antioxidant functions.关键词：Qilong Quyu Decoction;cerebral ischemia-reperfusion;oxidative stress;inflammatory response
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发布时间：2022-08-29 - 摘要：Objective： To explore the mechanism of Luhong Formula preventing myocardial ischemia-reperfusion injury （MIRI） in rats.MethodsForty rats were randomly divided into control group， model group， low dose group of Luhong Formula， medium dose group of Luhong Formula and high dose group of Luhong Formula， 8 in each group. The control group and the model group were given 0.9% NaCl solution by gavage， and the rats in Luhong Formula groups of different dosage were given different doses of Luhong Formula by gavage for 7 days. Twelve hours after the last gavage， the rat ischemia-reperfusion model was established by ligation of the left anterior descending branch for 30 min， and reperfusion for 24. After the model was copied， the samples were taken and the morphological changes of heart were observed by HE staining， and Serum creatine kinase （CK-MB）， lactate dehydrogenase （LDH）， troponin Ⅰ （cTn-Ⅰ）， reduced glutathione （GSH）， malondialdehyde （MDA） and superoxide dismutase （SOD） were detected. The expression of non-glycosylated XCT （SLC7A11） and glutathione peroxidase 4 （GPx4） proteins in myocardial tissue were detected by Western blot.Results①HE staining showed that compared with the control group， myocardial cells in model group were necrotic and edema， accompanied by inflammatory cell infiltration. All Luhong Formula groups of different dosage could improve myocardial injury， and the effect of medium and high doses groups was the best. ②The detection results of relevant myocardial injury markers showed that compared with the control group， the concentrations of CK-MB， LDH， cTn-Ⅰ and MDA increased significantly （P<0.05）， and the concentrations of GSH and SOD decreased significantly （P<0.05）. Luhong Formula groups of different dosage could reduce the contents of CK-MB， LDH， cTn-Ⅰ and MDA （P<0.05）， and increase the contents of GSH and SOD （P<0.05）， and the effect of medium and high doses groups was the best. ③The results of Western blot showed that compared with the control group， Luhong Formula groups of different dosage could promote the expression of SLC7A11 and GPX4 protein （P<0.05）.ConclusionLuhong Formula can alleviate myocardial ischemia-reperfusion injury， and its mechanism may be related to upregulating SLC7A11 / GPX4 pathway and inhibiting iron death.关键词：Luhong Formula;myocardial ischemia-reperfusion injury;oxidative stress;SLC7A11/GPx4 pathway;iron death;model rats;research of traditional Chinese medicine
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发布时间：2022-08-29 - 摘要：Objective： To explore the pathological mechanism of the imbalance of “liver-bile acid-intestinal microbiological axis” in non-alcoholic fatty liver disease （NAFLD） model mice induced by high-fat diet， verify the regulation of Cangju Qinggan Formula on the imbalance of “liver-bile acid-intestinal microbiological axis”， and further illustrate the mechanism of Cangju Formula in the treatment of NAFLD.MethodsThirty-two SPF male SD rats were randomly divided into blank group （N， n=5） and high-fat diet group （HFD， n=27）. Five mice in blank group were only given ordinary diet， and high-fat diet group was fed with hrgh-fat diet （cholestenol 1%，lard 10%， sucrose 20%） to induce NAFLD model. After 8 weeks of feeding， 25 rats in the high-fat diet group were randomly divided into model group （HFDM， n=5）， probiotic group （YSJ， n=5）， Cangju Formula high-dose group （CJFH， n=5）， Cangju Formula medium-dose group （CJFM， n=5）， Cangju Formula low-dose group （CJFL， n=5）. The contents of serum alanine aminotransferase （ALT） ， aspartate aminotransferase （AST） ， high density lipoprotein cholesterol （HDL-C）， low density lipoprotein cholesterol （LDL-C）， triglyceride （TG） and total cholesterol （TC） in serum of rats in each group were measured by automatic biochemical instrument. The degree of liver lesion and lipid deposition in rats were observed by HE staining. Using Illumina high-throughput sequencing method， the V3~V4 region of 16sr RNA of total DNA of rat fecal samples was sequenced and bioinformatics was analyzed.ResultsCompared with blank group， serum ALT and AST increased， TC， TG and LDL-C increased， and HLD-C decreased in model group， the differences were statistically significant （P<0.05）. Compared with the model group， serum ALT， AST and TC decreased in the probiotic group and Cangju Formula groups （P<0.05）， serum TG decreased in Cangju Formula medium and high dose group （P<0.05）， serum HDD-C increased in the probiotic group， Cangju Formula medium and high dose group， and serum LDL-C decreased in Cangju Formula high-dose group （P<0.05）. HE staining showed that hepatocytes in the model group presented diffuse vacuoles of different sizes and balloon-like changes， the infiltration of inflammatory cells in the portal area was obvious， and the hepatocytes were disordered with lots of lipid droplet of different sizes and dark color. All treatment groups could improve the damage degree of liver cells in NAFLD rats， with partially scattered vacuoles， and some liver cells returned to normal. The intestinal microflora of Cangju Formula groups were different from those of model group， and gradually approached to the state of the normal group.ConclusionCangju Formula can reduce the levels of ALT， AST， TC， TG， LDL-C in serum of rats， and increase the level of HDL-C in serum of rats. It can also improve the pathological status and lipid deposition of NAFLD rats. And it improves NAFLD mainly by improving the number of community species and gradually restoring the composition and structure of disturbed intestinal flora in NAFLD rats fed with high-fat diet to normal.关键词：Cangju Qinggan Formula;non-alcoholic fatty liver disease;intestinal flora;model rats;research of traditional Chinese medicine
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发布时间：2022-08-29 - 摘要：Objective： To explore the immune mechanism on the water extract of Bushen Jianpi Decoction in inhibiting hepatitis B virus in HepG2 cells transfected with plasmid pHBV1.3-pCR2.1.MethodsAfter the water extract of Bushen Jianpi Decoction intervened in HepG2 cells transfected with plasmid pHBV1.3-pCR2.1 in two different concentrations（250 μg/ml and 500 μg/ml）， HBsAg and HBeAg contents in cell supernatant were detected by ELISA. After the water extract of Bushen Jianpi Decoction intervened in HepG2 cells transfected with plasmid pHBV1.3-pCR2.1 in concentrations of 500 μg/ml， the expressions of TRL3， p-IRF3 protein were detected by WB， and the expression of IFN-β mRNA was detected by qRT-PCR. Bushen Jianpi Decoction intervened in HepG2 cells led that HepG2 cells were transfected with plasmid pHBV1.3-pCR2.1 and TLR3 siRNA down regulated the expression of TLR3. The expressions of TLR3 and p-IRF3 protein were detected by Western blot， and the expression of IFN-β mRNA was detected by qRT-PCR.ResultsHBsAg of Bushen Jianpi Decoction（500 μg/ml）was significantly decreased compared with that in Bushen Jianpi Decoction（250 μg/ml）and the model group（P<0.05，P<0.01）. HBeAg of Bushen Jianpi Decoction（500 μg/ml）was significantly decreased compared with that in Bushen Jianpi Decoction（250 μg/ml）and the model group（P<0.05，P<0.01）. Bushen Jianpi Decoction（500 μg/ml） enhanced TLR3 and p-IRF3 protein expressions， and promoted IFN⁃β mRNA expression in cell（P<0.01）. After Bushen Jianpi Decoction intervened in HepG2 cells transfected with plasmid pHBV1.3-pCR2.1， TLR3 siRNA inhibited TLR3 protein expression and p-IRF3 protein expression was decreased significantly and the expression of intracellular IFN⁃β mRNA expression was decreased significantly.ConclusionThe water extract of Bushen Jianpi Decoction can inhibit hepatitis B virus replication by enhancement of TLR3 effect in HepG2 cells transfected with plasmid pHBV1.3-pCR2.1 and promotion of intracellular IFN-β secretion.关键词：water extract of Bushen Jianpi Recipe;hepatitis B virus;Toll-like receptor 3
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发布时间：2022-08-29 - 摘要：Objective： To study the hepatoprotective effect of Zhizi Bopi Decoction on cholestasis mice induced by 1% cholic acid （CA） feeding， and explore the mechanism.MethodsC57BL/6 male mice were randomly divided into 3 groups （n=5）： normal control group， 1% CA model group， Zhizi Bopi Decoction group. Zhizi Bopi decoction group was given Zhizi Bopi Decoction by gavage （6 g/kg， body weight 0.1 ml per 10 g）， once a day， for consecutive 10 days. Normal control group and 1% CA model group were given 0.9% NaCl solution by gavage. All groups were fed normal feed for the first 3 days， and 1% CA model group and Zhizi Bopi Decoction group were changed to 1% CA diet on the 4th day after intragastric administration until the end of the experiment. The normal control group continued to feed with ordinary feed. After intervention， plasma alkaline phosphatase （ALP） and alanine aminotransferase （ALT） activities were detected， and liver histopathology was performed by H&E staining. Hepatic bile acid contents were measured by LC-MS/MS， and expression of NOD‑like receptors family pyrin domain containing 3 （NLRP3） inflammasome， adaptor （ASC） and N-terminal clipping body of gasdermin D （GSDMD-N） in mouse liver was quantified by Western blot.ResultsCompared with 1% CA model group， the activities of ALT and ALP in plasma in Zhizi Bopi Decoction group were significantly decreased， liver necrosis and inflammatory cell infiltration were alleviated， the contents of hepatic total bile acid， taurocholic acid （TCA） and taurodeoxycholic acid （TDCA） in liver were significantly decreased， and the protein expressions of NLRP3， ASC and GSDMD-N were down-regulated. The differences were statistically significant（P<0.05）.ConclusionZhizi Bopi Decoction can protect the liver of mice against 1% CA feeding-induced cholestatic liver injury， which may be related to alleviating hepatic bile acid accumulation， inhibiting the activation of NLRP3 inflammasome and reducing pyroptosis.关键词：Zhizi Bopi Decoction;cholestasis;bile acid;NLRP3 Inflammasome;pyroptosis
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发布时间：2022-08-29 - 关键词：Fuzheng Huayu Formula;hepatic fibrosis;CCl4;DMN;lymphocyte
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发布时间：2022-08-29 - 摘要：Objective： To investigate the mechanism of Bushen Rougan Formula against hepatic fibrosis from the perspective of angiogenesis.MethodsMale C57 mice were stratified randomly divided into normal group， model group， Bushen Rougan Formula group， Sorafenib group according to body weight. In order to induce liver fibrosis model， the model group， Bushen Rougan Formula group and Sorafenib group were intraperitoneally injected with 0.4% dimethylnitrosamine （DMN） once a day， three times a week for 8 weeks. The drug was administered at the 5th week of modeling. After 8 weeks， the mice were sacrificed， and serum and liver tissue samples were collected. The changes of body weight and death of mice were recorded. Serum alanine aminotransferase （ALT）， aspartate aminotransferase （AST） and albumin （ALB） contents were detected after sampling. The degree of hepatic fibrosis was evaluated by sirius red collagen staining and liver hydroxyproline （Hyp） contents. The expression of platelet endothelial cell adhesion molecule-1 （CD31） and vascular endothelial growth factor receptor 2 （VEGFR2） protein in liver tissue were used to evaluate hepatic angiogenesis.ResultsThe mortality rate of mice in the model group， Bushen Rougan Formula group and the Sorafenib group was 15.4%（2/13）， 0 and 7.7%（1/13）， respectively. The contents of serum ALT and AST in Bushen Rougan Formula group and the Sorafenib group were significantly decreased （P<0.05）， while the content of serum ALB was significantly increased （P<0.05）. Sirius red staining showed that the degree of fibrosis in Bushen Rougan Formula group and Sorafenib group was significantly reduced compared with the model group. Western blot and liver immunohistochemistry showed that CD31 and VEGFR2 protein expression were significantly down-regulated in Bushen Rougan Formula group and the Sorafenib group compared with the model group （P<0.05）.ConclusionBushen Rougan Formula can effectively improve DMN-induced hepatic fibrosis in mouse model， and its mechanism may be related to inhibition of angiogenesis.关键词：Bushen Rougan Formula;dimethylnitrosamine;hepatic fibrosis;angiogenesis
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发布时间：2022-08-29 - 摘要：Objective： To explore the effect and possible mechanism of Buqi Sanhan Formula on allergic rhinitis mouse model.MethodsBALB/c mice were randomly divided into blank control group， model group， Singulair group， low， medium and high concentration groups of Buqi Sanhan Formula， 10 mice in each group. AR mouse model was established by sensitization with OVA， and was treated with Buqi Sanhan Formula or singulair by gavage for 1 week. The symptomatic scores of the mice were recorded， and histological changes of nasal mucosa， serum levels of total immunoglobulin E （IgE）， OVA-specific IgE， interleukin-17A （IL-17A） and interleukin-33 （IL-33） were observed.ResultsBuqi Sanhan Formula could significantly reduce the nasal symptoms of allergic rhinitis mice， reduce the increase of body weight， thymus weight and thymus index caused by allergic rhinitis， significantly reduce the levels of serum total IgE and ova specific IgE， and reduce the contents of IL-17A and IL-33 in thymus.ConclusionBuqi Sanhan Formula has definite curative effect on AR mice. It may play a therapeutic effect on allergic rhinitis by reducing the level of serum total IgE， specific IgE and IL-17A.关键词：Buqi Sanhan Formula;allergic rhinitis;rhinallergosis
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发布时间：2022-08-29 - 摘要：Objective： This study aims to explore the molecular mechanism and material basis of Jianpi Bushen Decoction via network pharmacology.MethodsPotential targets of Jianpi Bushen Decoction on amyotrophic lateral sclerosis （ALS） were obtained by disease database and traditional Chinese medicine database. Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway and Gene Ontology （GO） enrichment analysis were performed using DAVID database. The protein-protein interaction （PPI） network was constructed using String database. And target network was analyzed and established by Cytoscape software.ResultsKEGG enrichment analysis showed that the mechanism of Jianpi Bushen Decoction on ALS may involve the cell survival （phosphatidylinositol 3-kinase/protein kinase B pathway， PI3K/Akt pathway）， inflammation （mitogen-activated protein kinase pathway， MAPKs signaling）， and calcium signaling pathway. GO enrichment analysis showed that Jianpi Bushen Decoction could regulate apoptosis， protein phosphorylation， protein binding and other processes. PPI network showed that glutamate ionic receptor family， MAPKs， and cysteine aspirate protease family were the main protein family. Target network analysis suggested that the main regulatory targets were apoptosis related genes， including cysteine protease 3 （CASP3）， cystatin C （CYCS） and tumor protein 53 （TP53）. Quercetin， luteolin， and kaempferol may be the potential active ingredients of Jianpi Bushen Decoction.ConclusionJianpi Bushen Decoction may treat ALS by inhibiting neuronal death and neuroinflammation through multiple pathways.关键词：Jianpi Bushen Decoction;amyotrophic lateral sclerosis;network pharmacology;glutamate receptors
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发布时间：2022-08-29 - 摘要：Objective： To investigate the effect of Miniao Paishi Mixture of our hospital on the formation of calcium oxalate renal calculi in rats， and explore its mechanism preliminarily.MethodsForty healthy male SD rats of SPF grade were randomly divided into normal group， model group， positive control group （potassium citrate） and Miniao Paishi Mixture group， 10 in each group. To establish calcium oxalate kidney stone model in rats， 1% ethylene glycol was given with drinking water， and 2% ammonium chloride was given by gavage. And each group was given corresponding drugs intervention. The 24 h urine volume， urinary oxalic acid content， urinary Ca2+， P3+， Mg2+ levels and serum BUN， Cr， Ca2+， P3+， Mg2+ levels of rats in each group were observed. Calcium oxalate crystal deposition and pathological changes in renal tissue sections were observed under microscope.Results①The 24 h urine volume and blood biochemical indexes： the 24 h urine volume of Miniao Paishi Mixture group and positive control group was higher than that of normal group and model group （P<0.05）. Compared with the model group， the levels of 24 h urinary oxalic acid， Ca2+ and P3+ in the blood and urine in the Miniao Paishi Mixture group and the positive control group were significantly lower （P<0.05）. Moreover， those in the Miniao Paishi Mixture group was lower than the positive control group （P<0.05）. ②Comparison of serum and urinary Mg2+ levels： the positive control group and Miniao Paishi Mixture group were higher than model group （P<0.05）. There was no significant difference in Mg2+ level in blood and urine between positive control group and Miniao Paishi Mixture group （P>0.05）. ③Renal function： the Miniao Paishi Mixture group and positive control group were significantly better than the model group （P<0.05）. ④Pathological change： compared with the model group， the deposition area of calcium oxalate crystals in the renal tissue was significantly reduced and the renal tubular edema decreased in the Miniao Paishi Mixture group and the positive control group.ConclusionMiniao Paishi Mixture can promote the excretion of oxalic acid， decrease the levels of calcium and phosphorus in blood and urine， increase the level of magnesium in blood， thus inhibit the formation of renal calcium oxalate crystal in experimental rats， and finally play a role in protecting renal function.关键词：Miniao Paishi Mixture renal calculus;calcium oxalate
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发布时间：2022-08-29 - 摘要：Objective： To investigate the effects of different concentrations of astragaloside IV （AS-Ⅳ） on the expression of endothelial nitric oxide synthase （eNOS）， nitric oxide （NO）， endothelin-1 （ET-1） and prostacyclin （PGI2） at different time points in human umbilical vein endothelial cells （HUVECs） treated with high glucose.MethodsHuman umbilical vein endothelial cells （HUVECs） were cultured in vitro and divided into control group， high glucose group （30 mmol/L）， 10 μmol AS-Ⅳ group， 20 μmol AS-Ⅳ group， 50 μmol AS-Ⅳ group and 100 μmol AS-Ⅳ group. Real time polymerase chain reaction （RT-PCR） was used to detect the expressions of eNOS mRNA at 12 h， 24 h and 48 h after AS-Ⅳ treatment in each group. ELISA was used to determine the concentrations of ET-1 and PGI2 in cell culture supernatant at 12 h， 24 h and 48 h， and nitrate reductase assay was used to detect the secretion of NO in the culture medium.ResultsCompared with the control group， the mRNA expression of eNOS， the secretion of PGI2 and NO decreased significantly， while the secretion of ET-1 increased significantly in the high glucose group（P<0.01）. Compared with the high glucose group， different concentrations of AS-Ⅳ increased the mRNA expression of eNOS， promoted the secretion of PGI2 and NO， and reduce the secretion of ET-1 to varying degrees（P<0.05，P<0.01）.ConclusionAS-Ⅳ can prevent endothelial cells from high glucose injury and improve endothelial cell function by increasing the expression of eNOS， promoting the secretion of NO and PGI2 and inhibiting the secretion of ET-1.关键词：Astragaloside Ⅳ;human umbilical vein endothelial cells;vasoactive substances
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发布时间：2022-08-29 - 摘要：Objective： To explore the potential active components and mechanism of Fructus Arctii on diabetic nephropathy.MethodsThe active components， targets and two-dimensional structure diagram of Fructus Arctii were collected through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP）. The related targets of diabetic nephropathy were obtained from TTD database， Drugbank database， GeneCards database and OMIM database， the repeated targets were eliminated， and then the Uniprot ID of each target was determined in combination with Uniprot database. On the basis of data from String database， the topological value of PPI network is calculated by the Network Analyzer and cytoHubba plugin of Cytoscape 3.7.0 software， and the topological network diagram for important targets is constructed. The network diagram of “active ingredient-target-pathway” is drawn. The core targets were imported into Metascape database for GO biological process enrichment analysis and KEGG analysis. The crystal structure of key target protein was downloaded from RSCB database， docked by AutoDock， and visualized by Pymol software.ResultsFructus Arctii has 8 active components and 80 main targets， and 34 targets were obtained after intersection with diabetic nephropathy targets. The core targets include transcription factor AP-1 （JUN）， tumor necrosis factor （TNF）， serine-threonine kinase （AKT1）， catenin protein beta 1 （CT-NNB1）， vascular endothelial growth factor A （VEGFA）， mitogen-activated protein kinase （MAPK8）， vascular cell adhesion molecule-1 （VCAM1）， nitric oxide synthase 2 （NOS2）， intercellular adhesion molecule-1 （ICAM1） and E-selectin （SELE）. Thirty GO items were obtained by GO enrichment analysis， and the top 10 main pathways were selected by KEGG pathway enrichment including fluid shear stress and atherosclerosis， advanced glycosylation end products （AGEs）-receptor of AGEs （RAGE） signaling pathway in diabetic complications， cancer pathway， tumor necrosis factor signaling pathway， Kaposi’s sarcoma-associated herpes virus infection， non-alcoholic fatty liver disease， Interleukin-17 signaling pathway， C-type lectin receptor signaling pathway， toxoplasmosis， proteoglycan in cancer， etc. It is speculated that AGE-RAGE signaling pathway in diabetic complications is the most closely related to diabetic nephropathy. The results of molecular docking showed that the active components of Fructus Arctii docked with multiple targets， and the binding energy between β-sitosterol and AKT1， kaempferol and TNF was low.ConclusionFructus Arctii may treat diabetic nephropathy through multiple components， multiple targets and multiple pathways.关键词：network pharmacology;molecular docking;Fructus Arctii;diabetic nephropathy
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发布时间：2022-08-29 - 摘要：Objective： To analyze the potential molecular mechanism of capsicum against colorectal cancer invasion and metastasis based on network pharmacology.MethodsThe main chemical components and their targets of capsicum were collected from TCMSP database. The disease targets related to colorectal cancer were obtained from GeneCards， PharmGkb， OMIM and TTD databases， and their intersection with the targets of active ingredients was the common targets of drug and disease. The protein interaction network map （PPI） of capsicum-colorectal cancer common targets was drawn by String database and Cytoscape 3.8.2， and the core targets were screened. GO enrichment analysis and KEGG pathway analysis were performed on key targets using DAVID database.ResultsThere were 260 common targets and 18 core genes including IGF1R， MAP2K1 and CHEK1. Pathway analysis showed that capsicum was involved in the regulation of several colorectal cancer related pathways， mainly involving the PI3K-AKT signaling pathway， MAPK signaling pathway， Ras signaling pathway and other classic tumor signaling pathways.ConclusionCapsicum regulates multiple targets， and the same target participates in multiple biological processes and signaling pathways. Capsicum may play an anti-invasion and metastasis role in colorectal cancer through multiple targets and pathways.关键词：network pharmacology;capsicum;colorectal cancer;mechanism
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发布时间：2022-08-29 - 摘要：Objective： The network pharmacology was used to screen the active components of Fukangling and their targets and pathways on triple negative breast cancer stem cells， and explore the molecular mechanism.MethodsTCMSP database， TCMID database and BATMAN-TCM database were used for screening active components of Fukangling and related targets. Disease-related genes were collected through GeneCards database and GEO database， and target genes were obtained by intersection. The protein interaction （PPI） network was constructed using String database. The key targets of Fukangling were analyzed by Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） using DAVID database.Results108 active ingredients of Fukangling and 446 corresponding target genes were obtained， and there were 49 genes associated with triple negative breast cancer stem cells. GO analysis showed that the gene function of Fukangling active compounds mainly manifested in biological progresses such as signal regulation， cell stimulation response， oxidative stress response， apoptosis， inflammatory response， and response to cytokine stimulation. Its anti-recurrence and metastasis against triple negative breast cancer is mainly through biological processes like regulating cell proliferation and migration. KEGG analysis showed that it mainly exerts therapeutic effects by regulating tumor necrosis factor （TNF） signaling pathway， mitogen-activated protein kinase （MAPK） signaling pathway， nuclear transcription factor-κB （NF-κB） signaling pathway， tumor pathway， epidermal growth factor receptor （EGFR） tyrosine kinase inhibitor resistance and so on.ConclusionThe targets， material basis and molecular mechanism of Fukangling on breast cancer stem cells， and its molecular mechanism were predicted.关键词：Fukangling;triple negative breast cancer stem cells;network pharmacology;pathway;molecular mechanism
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发布时间：2022-08-29 - 摘要：Objective： To analyze the potential targets and mechanisms of Erxian Decoction on mammary hyperplasia by network pharmacology.MethodsThe Chinese Medicine System Pharmacology Database and Analysis Platform （TCMSP） was used to screen the active ingredients of Erxian Decoction and their targets. Then the GeneCards database was used to search for the relevant targets of mammary hyperplasia. Map disease targets and drug targets， and predict the interactions of key targets between them through String database. Construct the network diagram of “drug-active component-action target”. And key targets were analyzed by Gene Ontology （GO） enrichment analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis， to analyze the potential mechanism of Erxian Decoction on mammary hyperplasia.ResultsA total of 85 active ingredients of Erxian Decoction， 215 targets of active ingredient， 4 775 targets of mammary hyperplasia， 175 intersection targets of active ingredients and diseases were obtained. The protein-protein interaction （PPI） core network contains 10 targets， and the top 10 GO enrichment results and the top 20 KEGG enrichment results were obtained.ConclusionErxian Decoction acts on ESR1， TP53， AKT1 and other targets through quercetin， kaherol， β -sitosterol and other active ingredients， and directly or indirectly regulates the activity of estrogen by regulating PI3K-AKT， MAPK and other signal pathways， thus exerts therapeutic effects of treating mammary gland hyperplasia.关键词：Erxian Decoction;mammary hyperplasia;estrogen;network pharmacology
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发布时间：2022-08-29 - 摘要：Objective： To explore the mechanism of Yougui Pill in the treatment of DED by observing the effect of Yougui Pill on oxidative stress in corpus cavernosum tissue of diabetic erectile dysfunction （DED） rats.MethodThe diabetic rat model was established by intraperitoneal injection of streptozotocin （STZ） in mature male SD rats. The erectile function of rats was observed by apomorphine （APO） test and the DED model was screened. DED rats were divided into model group （n=10） and Yougui Pill group （n=10）， and 10 of the most robust male SD rats were selected as control group. Eight weeks after administration， the erection of rats was observed by apomorphine test， the blood of tail vein was taken to determine the level of glucose. The corpus cavernosum tissue of rats were taken， of which the concentration of malondialdehyde （MDA）， the activity of superoxide dismutase （SOD） and glutathione reductase （GSH-Px） were determined， and the apoptosis of endothelial cells were determined by TUNEL staining. The protein expression levels of nuclear erythroid 2-related factor 2 （Nrf2） and heme oxygenase-1 （HO-1） were measured by Western blot.Result①Compared with the control group， the blood glucose level increased， the erectile function decreased， the antioxidant capacity decreased， and the cell apoptosis increased in the model group. ②Compared with the model group， in Yougui Pill group， the total number of erections increased， the erectile latent time decreased， and the blood glucose level had no significant difference， but the content of MDA decreased significantly （P<0.05）， the activity of SOD and GSH-Px as well as the expression of Nrf2 and HO-1 protein increased significantly （P<0.05）， and the cell apoptosis decreased.ConclusionYougui Pill may reduce oxidative stress injury and apoptosis of corpus cavernosum endothelial cells in DED rats by activating Nrf2-ARE signal pathway， thus improving diabetic erectile dysfunction.关键词：Yougui Pill;diabetic erectile dysfunction;oxidative stress;apoptosis;Nrf2-ARE
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发布时间：2022-08-29 - 摘要：Objective： To observe the effect of icariin on iron metabolism in mature osteoclasts and explore its possible mechanism.MethodsOsteoclast model was established and randomly divided into control group， low concentration icariin group （0.1 μmol/L）， medium concentration icariin group （1 μmol/L） and high concentration icariin group （10 μmol/L）. After the intervention， CCK-8 was used to determine the proliferation ability of cells in each group， and appropriate drug concentration was selected for subsequent experiments. The experimental groups were added with appropriate concentration of icariin-NAC （oxidant）. The differentiation level of osteoclasts was measured by tartrate resistant acid phosphatase （TRAP） specific staining. The levels of ROS and ferrous ions in osteoclasts were detected by reactive oxygen species （ROS） kit and ferrous ion fluorescent probe. The levels of GSH and GSH-Px in osteoclasts were detected by glutathione （GSH） and glutathione peroxidase （GSH-Px） kits. And the related protein expression of Nrf2/HO-1/GPX4 signal pathway in osteoclasts were measured by reverse transcription polymerase chain reaction （RT-PCR） and Western blot.ResultsIcariin inhibited the formation of osteoclasts in a dose-dependent manner. High concentration icariin had the strongest inhibitory effect on the activity of osteoclasts （P<0.05）. Compared with the control group， icariin increased the levels of ROS and ferrous ions in osteoclasts， and decreased the levels of GSH and GSH-Px. Icariin up-regulated oxidative stress-related Nrf2/HO-1 signal transduction pathway and promoted the gene expression of Nrf2， HO-1， inhibited the expression of GPX4 gene and transcriptional protein related to iron metabolism in osteoclasts.ConclusionIcariin may promote the changes of oxidative stress in osteoclasts， increase the level of ROS in osteoclasts， increase the level of ferrous ions in osteoclasts， down-regulate GPX4 gene expression related to iron metabolism by up-regulating Nrf2/HO-1 signal transduction pathway， in order to regulate the level of iron metabolism in osteoclasts and increase its oxidative stress injury， which inhibits osteoclast formation and bone resorption.关键词：icariin;osteoclast;iron metabolism;Nrf2/HO-1/GPX4 signal pathway;cell differentiation
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发布时间：2022-08-29 - 摘要：Objective： To explore the intervention mechanism of modified Liangge Powder （LGS） in sepsis-associated acute lung injury （ALI）/acute respiratory distress syndrome （ARDS） mice based on the immune regulation of vitamin D.MethodsSeventy-two 8-week-old male C57 mice were randomly divided into sham operation group， model group and LGS group. The drugs were administered continuously for 5 days， then the sepsis-associated ALI/ARDS model was established 1 h after the last administration by CLP. Twelve mice in each group were selected to observe the 7-day survival rate. The remaining mice were killed 24 h after operation， and the serum was collected. The inflammatory factors interleukin-6 （IL-6）， tumor necrosis factor-α （TNF- α） and 25-hydroxyvitamin D3 ［25（OH）D3］ were detected by enzyme linked immunosorbent assay （ELISA）.Results①Compared with the sham operation group， the 7-day survival rate of the model group was significantly decreased （P<0.05）， the serum levels of inflammatory factors IL-6 and TNF-α were significantly increased （P<0.05）， and the level of 25（OH）D3 was significantly decreased （P<0.05）. ②Compared with the model group， the 7-day survival rate of was significantly improved （P<0.05）， the serum levels of inflammatory factors IL-6 and TNF-α were significantly decreased （P<0.05）， and level of 25（OH）D3 was significantly increased （P<0.05） in the LGS group.ConclusionLGS may intervene the sepsis-associated ALI/ARDS based on the immune regulation of vitamin D.关键词：modified Liangge Powder;sepsis;vitamin D;inflammatory factors
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发布时间：2022-08-29 - 摘要：Objective： A rapid quantitative detection method for 12 chemical constituents in different genera of Celastraceae was established by ultra-high-performance liquid-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry （UHPLC-Q/Exactive）.MethodsDifferent genera of Celastraceae are crushed， sieved， and extracted with methanol ultrasonically. UPLC®HSS T3 column （2.1 mm×100 mm， 1.8 μm） was used for gradient elution with the mobile phase consists of 0.1% formic acid aqueous solution as the aqueous phase and acetonitrile as the organic phase， and the detection was performed with quadrupole/electrostatic field orbitrap high-resolution mass spectrometry with electrospray ion source in positive ion mode， and internal standard method is used for quantification.ResultsThe method had good linearity and wide linear range. The 12 chemical components all had good linear relationship in the range of 0.8 ~ 2 000 ng/ml， R2>0.99. The sample spiked recoveries were 97.1%~108.0%， and the relative standard deviations were 0.1%~1.2%.ConclusionThis method has the advantages of rapidness， simple operation and high accuracy. It can be used for the rapid quantitative analysis of the constituents of Celastraceae， which can better promote the rational use of Celastraceae expand the available classes of herbs， and provide a certain basis for scientific development of Chinese medicine resources.
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发布时间：2022-08-29 - 摘要：Objective： To investigate and analyze the content of phthalic acid esters （PAEs） in traditional Chinese medicine （TCM） decoction packaging film materials in Shanghai.MethodsA total of 30 batches of packaging film materials were selected from Shanghai TCM decoction service companies by random sampling survey method. Then the content of PAEs in TCM decocting packaging film was determined by gas chromatography-mass spectrometry （GC-MS）.ResultsThe linear range of each component was 0.01~5.00 mg/L， between which the linear relationship was good. The correlation coefficient was >0.998， and the detection limit was 0.002 mg/L. The standard curve and correlation coefficient showed a good linear relationship from 0.01 to 5.00 mg/L， the correlation coefficient was >0.998， the detection limit was 0.002 mg/L. According to the relevant regulations， the qualified rate of PAEs content in 30 batches of TCM decoction packaging film was only 20%. The main reason was that the content of bis（2-ethylhexyl）phthalate， dibutyl phthalate ， diisobutyl phthalate and other PAEs exceed the standard.ConclusionThe GC-MS method meets the requirement for determining PAES plasticizer. The content of PAEs in some packaging film materials of TCM decoctions exceeds the standard in Shanghai， which brings hidden dangers to the quality of the decoctions and the health of the patients. It is expected that the relevant departments should strengthen and standardize the supervision of plastic packaging film materials in TCM decoction industry.关键词：GC-MS;traditional Chinese medicine decoction;packaging film material;phthalic acid esters
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发布时间：2022-08-29
Experiment Research
- 摘要：Objective： To learn more about work stress， coping style and psychological health level of young medical staff in a Grade A tertiary hospital， explore related influencing factors， provide data support for improving the psychological health level of young medical staff， and put forward corresponding countermeasures and suggestions.Methods194 young medical staff in a Grade A tertiary hospital of traditional Chinese medicine were investigated by work stress scale， coping style scale and symptom checklist. Descriptive statistics， correlation analysis and multiple regression analysis were used to obtain the factors affecting psychological health level.Resultsthe average score of work stress of all the young medical staff was （88.79±16.05）， and the dimensions with the highest score were workload and external environment. The average score of coping style was （88.79±16.05）， and the dimensions with the highest score were problem solving and help seeking. The average score of psychological health was （143.95±11.86）， mainly manifested as compulsion and interpersonal sensitivity. Workload， career development， interpersonal relationship in work stress and self-blame in coping style are positively correlated with psychological health level. Workload， career development， interpersonal relationship in work stress， self-blame and avoidance in coping style have significant positive predictive effect on SCL-90， while problem solving has significant negative predictive effect on SCL-90.Conclusionyoung medical staff are under great work pressure. Work stress can be relieved from workload， external environment and interpersonal relationship； young medical staff can be guided and trained to adopt mature coping styles to promote the psychological health of young medical staff.关键词：young medical staff;work stress;coping style;psychological health
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发布时间：2022-08-29 - 摘要：In recent years， the policy of zero mark-up and centralized volume procurement of drugs and consumables has been proceeded orderly， and the price medical services has been adjusted in multiple rounds， which has promoted the improvement of medical services and the progress of medical technology. However， the medical service price system which is service-oriented， focused on technical services and adaptive to clinical diagnosis and treatment needs to be improved. Taking a number of public hospitals of Traditional Chinese Medicine in Shanghai as the research objects， according to the charging data of traditional Chinese medicine medical service items， a pricing mechanism based on the elements and characteristics of the traditional Chinese medicine services is proposed， which provides ideas for the price adjustment by medical insurance bureau and other government departments.关键词：elements and characteristics of traditional Chinese medicine service items;medical services;pricing mechanism
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发布时间：2022-08-29 - 摘要：The reform of DRG and DIP medicare payment system is intensive， and disease entities cost accounting energizes cost control， which becomes an important part of hospital fine management. Cost accounting of disease groups formed based on DRG and DIP， as well as its application is carried out relatively rare in traditional Chinese medicine hospitals. Based on the analysis of the advantages and disadvantages of the current disease cost accounting methods， a disease group cost accounting method based on “comprehensive index method” is proposed， whose application value is explored in traditional Chinese medicine hospitals， hoping to provide help for the lean operation of traditional Chinese medicine hospitals.关键词：hospital fine management;disease entities cost accounting;comprehensive index method
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发布时间：2022-08-29
Management Science
- 摘要：Patchouli alcohol is an important active ingredient of traditional Chinese medicine Pogostemonis Herba. In recent years， a variety of promising medicinal activities of patchouli alcohol have been reported， including influenza virus treatment， anti-inflammatory， regulating intestinal flora treatment of chronic intestinal diseases， anti-depression， analgesia， improving acute brain injury， antitumor， resisting metabolic related diseases， etc. This review will summarize the progress of the experimental research on patchouli alcohol in diseases， and try to analyze the existing problems in the current research， to provide a reference for further research.关键词：patchouli alcohol;Pogostemonis Herba;active ingredients;experimental research
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发布时间：2022-08-29 - 摘要：This article reviews the clinical application and experimental research progress of Mufangji Decoction at domestic and abroad in recent years. It mainly treats Gejianzhiyin. And modern clinical application and experimental research of Mufangji Decoction mainly focus on cardiovascular diseases， bone and joint diseases， edema and effusion， etc.关键词：cardiovascular diseases;bone and joint diseases;edema and effusion Mufangji Decoction;clinical application;experimental research
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发布时间：2022-08-29 - 摘要：Non-alcoholic fatty liver disease （NAFLD）， a metabolic stress liver injury closely related to insulin resistance and genetic susceptibility， has been the main chronic liver disease in our country. However， there is still a lack of effective western medicine for NAFLD. Recently， classical prescriptions have been applied in the treatment of NAFLD and achieved preferable efficacy. According to the classification of therapeutic principle and method， this paper summarizes relevant clinical and basic researches on the application of classical prescriptions （Dachaihu Decoction， Xiaochaihu Decoction， Linggui Zhugan Decoction， Dahuang Zhechong Pill， Biejiajian Pills， Yinchenhao Decoction， etc） in the treatment of NAFLD in recent years.关键词：non-alcoholic fatty liver disease;traditional Chinese medicine;classical prescription
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发布时间：2022-08-29 - 摘要：In the Ming Dynasty when Chinese herbal medicine was prevalent， the medicinal value of corn and its various parts were continuously explored， among which， the effective components of corn silk were complex and had both medical and food effects， which enriched the connotation of traditional Chinese medicine and played a certain role in promoting the development of traditional Chinese medicine in later generations. According to the literatures， corn silk has the functions of diuresis， heat relief， calming liver， choleretic action， broadening intestines and lowering Qi， etc. Modern pharmacological research has confirmed that corn silk and its active ingredients have anti-inflammatory， antioxidant， hypoglycemic， hypotensive， anti-tumor effects and so on. In clinical practice， corn silk is also widely used in various clinical departments， and has a broad prospect of development and utilization.关键词：corn silk;modern pharmacology;clinical application
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发布时间：2022-08-29 - 摘要：Due to the characteristics of tumor microenvironment and biological barrier， chemotherapy， immunity， traditional Chinese medicine and other drugs are difficult to penetrate and destroy tumor genetic material. Polypeptide polymerized nanodrugs have the characteristics of easy assembly， degradability and targeting， and the delivery system mediated by polypeptide polymerized nanodrugs has become the main research direction of tumor targeted drug release. This paper summarizes the recent progress of polypeptide nanodrugs that use multi-target combination and response of nanodrug delivery system， to tumor targeted release， improve the precise penetration curative efficacy of anti-tumor drugs such as chemotherapy， immunity and traditional Chinese medicine， and reduce oral absorption insoluble， toxic and side effects. This field will also become the main development direction of antitumor drug research in the future.关键词：nanoparticles;polypeptide;nanodrug delivery system;tumor;targeted therapy
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发布时间：2022-08-29 - 摘要：This paper reviews the research progress of metabonomics on type 2 diabetes mellitus and its associated complications， and analyzes the role of metabonomics in diabetic nephropathy， diabetic retinopathy， diabetic neuropathy and diabetic vasculopathy. It is believed that traditional Chinese medicine has unique advantages in the treatment of diabetes and the delaying the occurrence and development of complications. And through metabolomics， it has realized the early diagnosis of diabetes and related complications， which provides a possibility for delaying the occurrence and development of complications.关键词：metabolomics;type 2 diabetes diabetic;complications;traditional Chinese medicine therapy
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发布时间：2022-08-29
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