最新刊期
期 5 ， 2022
- 摘要：Seasonal influenza is an acute respiratory infectious disease that is easy to spread among people. Seasonal influenza virus can spread all over the world and can infect people of any age group. The epidemic of seasonal influenza has brought a huge disease burden and economic burden to the world. The thought of “Preventive treatment of disease” in traditional Chinese medicine （TCM） has important guiding significance for the prevention of infectious diseases represented by seasonal influenza. In order to standardize TCM prevention method of seasonal influenza and to form a local expert consensus， this research conducted three rounds of expert surveys as well as enquiries anonymously through Delphi method， optimizing and enriching the questionnaire items gradually. Finally， consensus was reached on 26 items of prevention and treatment of seasonal influenza in two parts， namely community residents and medical institutions， and the “TCM prevention scheme for seasonal influenza （Shanghai）” was initially formed.关键词：seasonal influenza;prevention;traditional Chinese medicine;preventive treatment of disease;Delphi method
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发布时间：2022-11-01
Expert Consensus
- 摘要：Focusing on the important issues of the discipline of “disease syndrome correlation， formula syndrome correspondence”， the research idea of “disease-syndrome-effect” was once proposed. Nearly two decades of research practice shows that this should be one of the effective ways to realize the inheritance and innovation of traditional Chinese medicine. Making full use of the research results of modern medicine and life science， studying the correlation between syndrome pathogenesis and “disease and formula”， and clarifying the pathological basis of disease formula and syndrome are the key scientific issues in syndrome research. Based on the results of previous studies， the authors analyzed the anti-liver fibrosis effects of Yiqi Buxu Huangqi Decoction， which are characterized by inhibiting the transdifferentiation of hepatic stellate cells （HSCs）into myofibroblasts， and hepatic progenitor cells（HPCs）into bile duct epithelial cells， as well as regulating the abnormal metabolism of bile acids. The key pathological basis of Qi deficiency pathogenesis of liver cirrhosis deduced from the effect basis of effective formulas may be related to the abnormal transdifferentiation of HSCs and HPCs （changes of cell shape and function） and abnormal bile acid metabolism. It provides a development idea for the combination of “disease-formula （effect）-syndrome” to explore the pathobiological basis of the pathogenesis of traditional Chinese medicine formulas and syndromes， and to discover the new uses of classical formulas.关键词：syndrome pathogenesis;pathology of formulas and syndromes;liver cirrhosis;Huangqi Decoction;ductular reaction;bile acid metabolism
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发布时间：2022-11-01
Scholar Forum
- 摘要：Objective： To observe the clinical efficacy of Xinglou Chengqi Decoction in the treatment of cognitive impairment after ischemic stroke with phlegm-heat and bowel-repletion type based on the theory of “brain-gut interaction”.MethodsA total of 144 patients of cognitive impairment after ischemic stroke with phlegm-heat and bowel-repletion type were included， and randomly divided into the treatment group and control group， 72 cases in each group. All the patients were treated with basic treatment of ischemic stroke and donepezil hydrochloride tablets orally. Based on above， the patients in the control group were orally treated with lactulose oral solution and the patients in the treatment group were orally treated with Xinglou Chengqi Decoction， with a course of 30 d. Before treatment and after 30 and 90 d of treatment， the scores of mini-mental state examination （MMSE） in the two groups were evaluated. After 90 d of treatment， the effect of cognitive function improvement was compared between the two groups. Before treatment and after 30 and 90 d of treatment， the expression levels of serum 5-hydroxytryptamine （5-HT）， calcitonin gene-related peptide （CGRP） and motilin （MTL） were detected.ResultsDuring the course of research， 5 patients in the control group and 7 patients in the treatment group were eliminated and shed off， finally 67 patients in the control group and 65 patients in the treatment group were included in the statistical analysis. ①After 90 d of treatment， the scores of MMSE in the two groups were increased compared with those before treatment （P<0.05）， and the score of the treatment group was higher than that of the control group （P<0.05）. ②After 90 d of treatment， the total effective rates on cognitive function improvement of the treatment group and the control group were 89.23% and 74.63% respectively， and the effect of the treatment group was better than that of the control group （P<0.05）. ③After 30 d of treatment， the levels of 5-HT， CGRP and MTL in the two groups were increased compared with those before treatment （P<0.05）， and the levels of above indexes in the treatment group were higher than those in the control group （P<0.05）. After 90 d of treatment， the levels of 5-HT， CGRP and MTL in the treatment group were increased compared with those before treatment （P<0.05）， the levels of 5-HT and MTL in the control group were also increased compared with those before treatment （P<0.05）， and the levels of 5-HT， CGRP and MTL in the treatment group were higher than those in the control group （P<0.05）.ConclusionBased on the conventional western medicine， Xinglou Chengqi Decoction can effectively improve the cognitive function in patients of cognitive impairment after ischemic stroke with phlegm-heat and bowel-repletion type， and regulate the expression levels of serum brain-gut peptide indexes， thus play the effect of “brain-gut interaction”.关键词：ischemic stroke;cognitive impairment;Xinglou Chengqi Decoction;brain-gut interaction;brain-gut peptide
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发布时间：2022-11-01 - 摘要：Objective： To observe the clinical effect of Shenjing Fuyuan Formula in preventing the recurrence of minor ischemic stroke （IS）.MethodsA multi-center， randomized， double-blind， controlled study was applied. A total of 180 patients of minor IS with Qi-yin deficiency and phlegm-stasis stagnation type were included， and randomly divided into the treatment group and control group， 90 cases in each group. The patients in the two groups were treated with conventional western medicine. Based on above， the patients in the treatment group were orally treated with Shenjing Fuyuan Formula granules， and the patients in the control group were orally treated with Shenjing Fuyuan Formula granule placebo， with a course of 270 d. After 90， 180 and 270 d of treatment， the occurrences of IS， transient ischemic attack （TIA） and other ischemic events （including myocardial infarction， acute coronary syndrome and so on） were recorded， and the recurrence rate of IS， the incidence of TIA and so on were calculated and compared in the two groups.ResultsIn the course of study， 10 patients in the treatment group and 10 patients in the control group were eliminated and shed off， finally 80 patients in the treatment group and 80 patients in the control group were included in the statistical analysis. ①After 90， 180 and 270 d of treatment， the recurrence rates of IS in the treatment group were 3.75%， 3.75% and 5.00% respectively， and the recurrence rates of IS in the control group were 2.50%， 13.75% and 16.25% respectively. After 180 and 270 d of treatment， the recurrence rates of IS in the treatment group were significantly lower than those in the control group （P<0.05）. ②After 90， 180 and 270 d of treatment， the incidences of TIA in the treatment group were 5.00%， 6.25% and 6.25% respectively， and the incidences of TIA in the control group were 6.25%， 16.25% and 17.50% respectively. After 180 and 270 d of treatment， the incidences of TIA in the treatment group were significantly lower than those in the control group （P<0.05）. ③In the course of treatment， new-onset acute myocardial ischemia occurred in 2 patients in the control group and no other ischemic diseases occurred in the treatment group. ④After 90， 180 and 270 d of treatment， the incidences of total ischemic events in the treatment group were 8.75%， 10.00% and 11.25% respectively， and the incidences of total ischemic events in the control group were 10.00%， 28.75% and 31.25% respectively. After 180 and 270 d of treatment， the incidences of total ischemic events in the treatment group were significantly lower than those in the control group （P<0.05）.ConclusionShenjing Fuyuan Formula can effectively prevent the recurrence of minor IS and the occurrence of TIA， reduce the incidence of total ischemic events， and the effect becomes more obvious with the prolongation of the treatment time.关键词：ischemic stroke;Shenjing Fuyuan Formula;secondary prevention
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发布时间：2022-11-01 - 摘要：Objective： Taking prednisone as the control， to observe the clinical effect of Yajia Formula in the treatment of severe subacute thyroiditis （SAT）.MethodsA total of 76 severe SAT patients with heat-toxic congestion and blood-heat stagnation type were selected and randomly divided into the treatment group and control group， 38 cases in each group. The patients in the treatment group were orally treated with Yajia Formula granules and the patients in the control group were orally treated with prednisone acetate tablets， with a course of 8 weeks. The clinical effect and the Chinese medical syndrome effect were evaluated in the two groups. The relief time and disappearance time of fever and thyroid pain and the improvement of thyroid ultrasonography were compared between the two groups. The incidences of adverse reactions were compared between the two groups. Follow-up for 3 months after the end of the treatment course， the recurrence rates of the two groups were compared.ResultsIn the course of the research， 3 patients in the treatment group and 3 patients in the control group were eliminated or shed off， finally 35 patients in the treatment group and 35 patients in the control group were included in the statistical analysis. ①After treatment， the total clinical effective rates of the treatment group and the control group were 91.43% and 85.71% respectively， and the effect of the treatment group was better than that of the control group（P<0.05）； after treatment， the total effective rates on Chinese medical syndrome in the treatment group and the control group were 91.43% and 62.86% respectively， and the effect on Chinese medical syndrome in the treatment group was better than that in the control group （P<0.05）. ②The relief time and disappearance time of fever and thyroid pain in the treatment group was significantly longer than that in the control group （P<0.05）. ③After treatment， the condition of thyroid ultrasonography in the two groups was significantly improved compared with that before treatment （P<0.05）， but there was no statistical difference between the two groups （P>0.05）. ④In the course of the research， the incidences of adverse reactions in the treatment group and the control group were 0% and 22.86% respectively， and the incidence of adverse reactions in the treatment group was significantly lower than that in the control group （P<0.01）. ⑤The recurrence rates of the treatment group and the control group were 0% and 36.67% respectively， and the recurrence rate of the treatment group was significantly lower than that of the control group （P<0.01）.ConclusionYajia Formula has better clinical effect in treating severe SAT patients with heat-toxic congestion and blood-heat stagnation type， and can improve the Chinese medical syndrome and the hypoechoic condition of thyroid ultrasonography with less adverse reactions and lower recurrence rate.关键词：severe subacute thyroiditis;heat-toxic congestion and blood-heat stagnation type;Yajia Formula;traditional Chinese medicine therapy
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发布时间：2022-11-01 - 摘要：Objective： To observe the effect of acupuncture intervention on endometrial peristalsis and pregnancy outcomes at peri-transfer period in patients of in vitro fertilization-embryo transfer （IVF-ET） undergoing frozen embryo transfer.MethodsA total of 150 patients who met the inclusion criteria and planned to undergo frozen-thawed embryo transfer （FET） were selected and divided into the treatment group 1， treatment group 2 and control group by random number table， 50 cases in each group. The patients in the control group were treated with conventional FET endometrial preparation. The patients in the treatment group 1 received acupuncture treatment from the 2nd day of menstruation to 1 d before transplantation on the basis of routine endometrial preparation， once every other day， and the treatment cycle was 1 menstrual cycle， in addition the patients received acupuncture treatment at 30 minutes before and after transplantation. On the basis of routine endometrial preparation， the patients in the treatment group 2 received acupuncture treatment at 30 minutes before and after transplantation. The implantation rate and clinical pregnancy rate of the three groups were compared. At 30 minutes before and after embryo transfer， the uterine peristalsis frequency （UPF） of all patients was detected and calculated， and the clinical pregnancy rates among different UPF patients were compared. The endometrial thickness， endometrial blood flow pulsatility index （PI） and resistance index （RI） of all patients were detected on the day of transplantation.ResultsDuring the study， 1 patient in the treatment group 1， 2 patients in the treatment group 2 and 1 patient in the control group shed off， finally 49 patients in the treatment group 1， 48 patients in the treatment group 2 and 49 patients in the control group were included in the statistical analysis. ①The clinical pregnancy rates of the treatment group 1， treatment group 2 and control group were 48.98%， 31.25% and 28.57% respectively， with no statistical difference among the three groups （P>0.05）. The implantation rates of the treatment group 1， treatment group 2 and control group were 32.18%， 17.65% and 18.52% respectively， and the implantation rate of the treatment group 1 was significantly higher than that in the treatment group 2 and the control group （P<0.05）. ②After transplantation， the UPF of the three groups was significantly increased compared with that before transplantation （P<0.05）， but the UPF of the treatment group 1 was significantly lower than that of the treatment group 2 and the control group （P<0.05）， and the UPF of the treatment group 2 was lower than that of the control group （P<0.05）， too. There were statistical differences on the clinical pregnancy rate among the patients in the different UPF groups （P<0.05）， and the clinical pregnancy rate of the group B （UPF>1 and ≤2 times/min） was the highest. ③On the day of transplantation， the endometrial thickness of the treatment group 1 was significantly thicker than that of the treatment group 2 and the control group （P<0.05）， and the endometrial blood flow PI of the treatment group 1 and treatment group 2 was significantly lower than that of the control group （P<0.05）.ConclusionAcupuncture intervention can effectively regulate the UPF at peri-transfer period in patients of IVF-ET undergoing frozen embryo transfer， improve the endometrial thickness and blood flow during the implantation window， and increase the implantation rate. In addition， the acupuncture intervention for 1 menstrual cycle has better clinical efficacy.关键词：in vitro fertilization-embryo transfer;frozen embryo transfer;acupuncture;endometrial peristalsis;pregnancy outcomes;randomized controlled trial
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发布时间：2022-11-01 - 摘要：Objective： To observe the effect of Baduanjin on the balance function in hospitalized patients with stable schizophrenia.MethodsA total of 76 inpatients with stable schizophrenia were selected and randomly divided into treatment group and control group， 38 cases in each group. The control group was given conventional treatment， and the treatment group was additionally given Baduanjin on this basis. Both groups were intervened for 12 weeks. The berg balance scale （Berg）， the timed up and go test （TUGT）， the positive and negative symptom scale （PANSS） and the insight and attitude to therapy questionnaire （ITAQ） were performed in both groups respectively before the intervention， 6 weeks after the intervention， and 12 weeks after the intervention.ResultsIn the course of the research， one patient in the control group was eliminated or shed off， thus finally 38 patients in the treatment group and 37 patients in the control group were included in the statistical analysis. The results of repeated measures analysis of variance showed that compared with the control group， the Berg score and ITAQ score of patients were improved who were treated with Baduanjin combined with conventional treatment， and there was an interaction between time and group （F=68.11， F=12.74， P<0.01）. And TUGT time and PANSS score were decreased， and there was an interaction between time and group （F=30.96， F=74.74， P<0.01）.ConclusionBaduanjin combined with conventional treatment can improve the balance function， mental state and insight of patients with schizophrenia， which has good clinical application value.关键词：schizophrenia;Baduanjin;balance function;mental state;insight
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发布时间：2022-11-01
Clinical Research
- 摘要：Objective： To bioconvert cholesterol to 7α-hydroxy-4-cholesten-3-one.MethodsBufo bufo gargarizens Cantor-derived 3β-hydroxysteroid dehydrogenase gene （Bg-HSD3B7）， cytochrome P450 reductase gene （Bg-CPR） and Rattus norvegicus-derived cytochrome P450 enzyme gene （Rn-CYP7A1） were amplified by PCR. Physicochemical properties were predicted by bioinformatics website ExPASy， TMHMM. The engineered bacterias were obtained by using different construction and combination methods. Whole-cell catalysis was performed using cholesterol as a substrate， and high-performance liquid chromatography was used to detect whether the intermediate product of 7α-hydroxycholesterol and the final product of 7α-hydroxy-4-cholesten-3-one were produced.ResultsThree genes were cloned and constructed into pETM6 vector in a monocistronic manner and E. coli engineered bacteria BL21(DE3) with Rn-CYP7A1, Bg-CPR and Bg-HSD3B7 genes was obtained. This engineered bacteria could convert cholesterol to 7α-hydroxy-4-cholesten-3-one by whole-cell catalytic method without any intermediate.ConclusionBg-HSD3B7 gene and Bg-CPR gene were cloned from Bufo bufo gargarizans Cantor and Rn-CYP7A1 gene was cloned from Rattus norvegicus. And the engineered E. coli with whole-cell catalytic function was constructed， which could catalyze cholesterol to 7-hydroxy-4-cholesten-3-one.关键词：Bufo bufo gargarizans Cantor;cholesterol;engineering bacteria;3β-hydroxysteroid dehydrogenase;cytochrome P450 reductase
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发布时间：2022-11-01
Discipline Frontiers
- 摘要：Objective： The changes of moisture and texture characteristics in the moistening process of Moutan Cortex were quantitatively characterized， so as to explore the scientific connotation of the traditional experience of Moutan Cortex moistening.MethodsThe dynamic change of water absorption during the moistening process of Moutan Cortex was evaluated by the water absorption kinetics curve. Low-field nuclear magnetic resonance technology was used to analyze the changes of water phase and distribution state during the moistening process of Moutan Cortex. The changes of texture characteristics of Moutan Cortex during moisturizing were measured by texture analyzer. HPLC was used to determine the content changes of active ingredients in the moisturizing process of Moutan Cortex.ResultsThe water absorption rate was increased with the increment of the moistening temperature， and was decreased with the increasing grade of the medicinal material. The water absorption kinetic model of different grades of Moutan Cortex at 10~30 ℃ was established to predict the water absorption rate of different moisturizing time at different temperatures. During the moistening process， the proportion of bound water and free water changed continuously， within 5 h， the proportion of bound water in high-grade Moutan Cortex was decreased from 92.8% to 14.3%， and the proportion of free water was increased from 7.2% to 85.7%， after 7 h， all was transformed into free water. Low field nuclear magnetic resonance imaging showed that water had evenly infiltrated inside the Moutan Cortex after moistening for 5 h and there was no dry core inside at this time. The analysis of texture characteristics showed that the hardness and compression work were decreased with the increase of moistening time， the hardness was decreased from 3612 g to 444 g and the compression work were decreased from 208 mJ to 23 mJ within 5 h， at which time the hardness of the medicinal material was moderate， and the texture characteristics tended to be stable after 5 h. The contents of paeonol and paeoniflorin were decreased gradually with the extension of moistening time， indicating that the longer moistening time， the more loss of medicinal substances.ConclusionWater absorption kinetics combined with modern technologies such as low-field nuclear magnetic resonance and texture analyzer can quantitatively characterize the moistening process of Moutan Cortex， which can provide data support for the judgment of the end point of Moutan Cortex moistening and process optimization.关键词：Moutan Cortex;Moistening;Water absorption kinetics;LF-NMR;Texture characteristics;content determination
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发布时间：2022-11-01
Prescription Research
- 摘要：Objective： To investigate the role of delta-opioid receptor （DOR） in electroacupuncture （EA） against retinal cell necroptosis caused by retinal ischemia-reperfusion induced by high intraocular pressure （HIOP）.MethodsFifty-five Wistar rats were randomly divided into five groups： blank control group， HIOP 6 h group， HIOP 12 h group， HIOP 24 h group and HIOP 72 h group. PI staining was used to detect the number of necroptotic cells in rats’ retina at the above timings， and Western blot was used to detect the expressions of p-Rip1 and p-Rip3. Another fifty-five Wistar rats were randomly divided into 5 groups： blank control group， HIOP group， EA+HIOP group， sham EA+HIOP group， and DOR antagonist+HIOP+EA group. Except for the blank control group， HIOP was used in the other groups to induce retinal ischemia-reperfusion in rats， and the ischemia lasted for 1 h， then reperfused for 24 h. DOR antagonist+HIOP+EA group received intravitreal injection of DOR antagonist Naltrindole 1 h before ischemia. At the beginning of reperfusion and 12 h after reperfusion， EA was performed at acupoints “Shuigou” and “Jingming” twice， 30 min each time. 24 h after reperfusion， the number of necroptotic cells in retina was detected by PI staining， and the expressions of p-Rip1 and p-Rip3 were detected by Western blot.ResultAfter HIOP for 6 h， 12 h， 24 h and 72 h， the number of retinal necroptotic cells was increased significantly， and reached a peak at 12 h. The protein expressions of p-Rip1 and p-Rip3 in the retina were also significantly increased， and the peak of p-Rip1 expression appeared at 12 h and the expression of p-Rip3 was the highest at 24 h. After 24 h of reperfusion， the number of necroptotic cells and the protein expressions of p-Rip1 and p-Rip3 in the EA+HIOP group were all significantly lower than those in the HIOP group. Compared with the HIOP group， there was no significant difference in the number of necroptotic cells or the protein expressions of p-Rip1 and p-Rip3 in the sham EA+HIOP group and the DOR antagonist+HIOP+EA group.ConclusionIn retinal ischemia-reperfusion injury induced by HIOP， EA at acupoints “Shuigou” and “Jingming” can significantly reduce retinal cell necroptosis and protect the retina， and the anti-retinal cell necroptosis effect of EA may be closely related to DOR.关键词：electroacupuncture;retinal ischemia-reperfusion injury;δ-opioid receptor;necroptosis
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发布时间：2022-11-01 - 摘要：Objective： To observe the intervention effect and possible mechanism of Qilongtian on bleomycin induced pulmonary fibrosis in mice.MethodsThirty-six C57BL/6J mice were randomly divided into control group， model group， Qilongtian low， medium and high dose group， pirfenidone group. After the establishment of mouse pulmonary fibrosis model， the control group and model group were administrated with 0.9% NaCl solution by gavage， and the Qilongtian group and pirfenidone group were administrated with Qilongtian and pirfenidone by gavage respectively. After 21 d of administration， the morphology and collagen deposition of mice lung were measured by HE and Masson staining. The content of hydroxyproline （HYP） was detected by alkali hydrolysis method. The expression levels of TGF-β， α-SMA， COL-Ⅰ， COL-Ⅲ， TNF-α， CCL2， FOSL1， MMP9， CXCL5， AREG mRNA were detected by qRT-PCR. The expression levels of TGF-β， α-SMA， COL-Ⅰ， COL-Ⅲ， TNF-α， IL-17R， p38MAPK， and p-p38MAPK protein were detected by Western blot and immunofluorescence double labeling method.Results①Compared with the control group， the lung tissues of mice in model group showed inflammatory cell infiltration and alveolar space expansion， and the collagen deposition was more （P<0.01）. Compared with the model group， Qilongtian each dose group inhibit the infiltration of inflammatory cells and the expansion of alveolar space， and collagen deposition was significantly reduced in pirfenidone group and Qilongtian each dose group （P<0.05， P<0.01）. ② Compared with the control group， the content of HYP in the model group increased （P<0.01）. Compared with the model group， the content of HYP in pirfenidone group and Qilongtian each dose group decreased （P<0.05， P<0.01）. ③ Compared with the control group， the protein and mRNA expression level of COL-Ⅰ， COL-Ⅲ， α-SMA， TGF-β， TNF-α in the model group increased （P<0.01）. Compared with the model group， the protein and mRNA expression levels of COL-Ⅰ， COL-Ⅲ， α-SMA， TGF-β， TNF-α in pirfenidone group and Qilongtian each dose group decreased （P<0.05， P<0.01）. ④Transcriptome sequencing analysis of mice lung tissues in the blank group and model group showed that differential genes were significantly enriched in IL-17/p38MAPK signaling pathway. ⑤Compared with the control group， the expression levels of CCL2， FOSL1， MMP9， CXCL5， AREG mRNA and IL-17R， p-p38MAPK protein increased in the model group （P<0.01）. Compared with the model group， the expression levels of CCL2， FOSL1， MMP9， CXCL5， AREG mRNA and IL-17R， p-p38MAPK protein decreased in pirfenidone group and Qilongtian each dose group （P<0.05， P<0.01）. Meanwhile， the effects of Qilongtian on the above mRNA and protein expression were dose-dependent， and high dose was more effective.ConclusionQilongtian can intervene pulmonary fibrosis by down regulating the expression of inflammation， collagen genes and proteins， alleviate pulmonary inflammatory response， reducing excessive deposition of pulmonary collagen， and its mechanism may be through inhibition of IL-17/p38MAPK signal pathway.关键词：Qilongtian;pulmonary fibrosis;transcriptome;IL-17/p38MAPK
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发布时间：2022-11-01 - 摘要：Objective： To study the effects of Rougan Formula on interleukin-22 （IL-22） secretion and IL-22 receptor 1 （IL-22R1） expression in the liver of concanavalin A （ConA） combined with 3,5-diethoxy carbonyl-1,4-dihydro-2,4,6-trimethylpyridine （DDC） induced liver fibrosis mice， and to explore the mechanism of Rougan Formula against liver fibrosis.MethodsSixty mice were randomly divided into control group， model group and Rougan Formula group， with 20 mice in each group. The mice in the model group and Rougan Formula group were injected with ConA through the tail vein once a week and fed with DDC diet. The mice in the control group were injected with equal volume of 0.9% NaCl solution through the tail vein and fed with normal diet. The mice in Rougan Formula group were given Rougan Formula granules by gavage （8.5 g/kg） once a day. The mice in control and model group were given equal volume of 0.9% NaCl solution by gavage. The Drug intervention lasted for 10 weeks. The mice were killed after 10 weeks， liver function， secretion of IL-22 and expression of IL-22R1 in liver were observed.Results①Compared with the control group， the expression of IL-22， IL-22R1， IL-10R2 and STAT3 mRNA in the liver of mice in the model group and Rougan Formula group significantly increased （P<0.01，P<0.001）. Compared with the model group， the expression of IL-22 and IL-10R2 mRNA in the liver of mice in the Rougan Formula group significantly increased （P<0.001）. ②Compared with the control group， the expression of IL-22R1 mRNA on the surface of hepatic stellate cells （HSCs） in the model group significantly increased （P<0.05）， the expression of STAT3 mRNA in HSCs significantly increased （P<0.05）， the expression of IL-22R1 and IL-10R2 mRNA on the surface of HSCs in the Rougan Formula group significantly increased （P<0.05，P<0.001）， and the expression of STAT3 mRNA in HSCs in the Rougan Formula group significantly increased （P<0.001）. Compared with the model group， the expression of IL-22R1 mRNA on the surface of HSCs and STAT3 mRNA in HSCs in the Rougan Formula group significantly increased （P<0.01，P<0.001）. ③Compared with the control group， the number of Th22 cells in the model group and Rougan Formula group significantly increased （P<0.001）. Compared with the model group， the number of Th22 cells in the Rougan Formula group significantly increased （P<0.01）.ConclusionRougan Formula can increase the secretion of IL-22 in the liver and the expression of IL-22R1 on the surface of HSCs， so as to play the role of anti-hepatic fibrosis.关键词：Rougan Formula;hepatic fibrosis;IL-22;IL-22R1
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发布时间：2022-11-01 - 摘要：Objective： To explore the mechanism of Mori Folium-Carthami Flos on type 2 diabetes based on network pharmacology.MethodsThe targets of drugs and disease were collected based on Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform （TCMSP） and Therapeutic Target Database （TTD）， and the network diagram of “active component-key target-pathway” was constructed by Cytoscape software. GO enrichment analysis and KEGG analysis were carried out based on DAVID 6.8 database to identify key targets and key components， and AutoDock Vina Software was used for molecular docking verification. Cell experiments were performed to verify the effects of key chemical components on insulin resistant model cells.Results①The results of network topology analysis showed that the key drug components might be baicalin and inophyllum E， and the key targets might be tumor necrosis factor （TNF） and AKT serine/threonine protein kinase 1（AKT1）. ②KEGG analysis showed that the anti-type 2 diabetes of Mori Folium-Carthami Flos was mainly associated with signal pathways such as insulin signaling pathway and adenosine-activated protein kinase （AMPK） pathway. ③Molecular docking showed that AKT1 and baicalin， TNF and inophyllum E had the best molecular docking effect. ④The cell experiments demonstrated that 20 μg/ml baicalin and 10 μg/ml inophyllum E were not toxic to HepG2 cells and could improve the insulin sensitivity of the cells in HepG2 model cells after intervention.ConclusionMori Folium-Carthami Flos may play a synergistic role in the treatment of type 2 diabetes through pathways such as insulin signal transduction pathway and insulin resistance related pathway， and the key pharmacodynamic components may be baicalin and inophyllum E.关键词：Mori Folium-Carthami Flos;type 2 diabetes;baicalin;AKT1;network pharmacology
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发布时间：2022-11-01 - 摘要：Objective： To explore the mechanism of Jianpi Formula in preventing disuse muscle atrophy in mice based on Akt/FOXO3a signaling pathway.MethodsForty-five male C57/BL6 mice were randomly divided into control group， model group and Jianpi Formula group. Disuse muscular atrophy mouse model was established by hindlimb unloading. Mice in control group and model group were given 0.2 ml 0.9% NaCl solution by gavage every day， and mice in Jianpi Formula group were given 0.2 ml Jianpi Formula suspension by gavage every day. After 28 d of drug administration， the body weight and wet muscle weight （accumulation of muscle， gastrocnemius muscle， soleus muscle， tibialis anterior muscle， extensor digitorum longus muscle and quadriceps femoris muscle） of mice were weighed. Moreover， qRT-PCR was used to detect IGF-1， PI3K， Akt， Atrogin and MuRF1 mRNA expression level， Western blot was used to detect p-Akt， p-FOXO3a， Akt and FOXO3a protein expression level in gastrocnemius muscle.Results①Compared with the control group， the grip strength/body weight ratio， wet weight/body weight ratio of muscles， cross-sectional area and diameter of muscle fiber in mice of the model group significantly decreased （P<0.05）. Compared with the model group， the grip strength/body weight ratio significantly increased （P<0.05）； the wet weight/body weight ratio of cumulative muscle， soleus muscle， tibialis anterior muscle and quadriceps femoris muscle significantly increased （P<0.05）； muscle fiber cross-sectional area significantly increased （P<0.05）. ②After the intervention of Jianpi Formula， IGF-1 and Akt mRNA expression significantly increased （P<0.05） in gastrocnemius muscle of mice， while Atrogin and MuRF1 mRNA expression significantly decreased （P<0.05）； p-Akt protein expression increased， p-FOXO3a protein expression decreased.ConclusionJianpi Formula may be prevent disuse muscular atrophy by inhibiting Akt/FOXO3a signaling pathway through up-regulating Akt phosphorylation.关键词：Jianpi Formula;disuse muscle atrophy;hindlimb unloading;grip strength;gastrocnemius muscle;Akt/FOXO3a signaling pathway
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发布时间：2022-11-01
Experiment Research
- 摘要：The concept of Zang-fu in the theory of Zang-xiang in tradional Chinese medicine（TCM）， some do not have modern anatomical attributes， while some are related to the physical organs and objective functions， that is， “Xing-zang”. Although the theory of Xing-zang TCM has certain anatomical properties， it is still quite different from modern anatomy. Under the background of the rapid development of modern imaging diagnosis technology， it is of great practicality and necessity to promote the theoretical study of Xing-zang in TCM. We should face up to the widespread anatomical properties of human viscera and organs， and treat the “Shen-zang” with non-anatomical attributes differently； in the understanding of “Xing-zang”， we can learn from modern anatomical achievements， and carry out TCM pathomorphological research， as well as develop TCM empirical medicine theory within feasible scope.关键词：Zang-xiang theory;Xing-zang;anatomy;basic theory of tradional Chinese medicine
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发布时间：2022-11-01 - 摘要：Ying-nutrients and Wei-defence and qi⁃blood are the basic substances that constitute human body and maintain human life activities. Although Ying-nutrients and Wei-defence and qi⁃blood have been described separately， the relationship between the two is rarely discussed. The syndromes and treatments of Huangqi Guizhi Wuwu Decoction are analyzed through “medicine group method”， the pathogenesis was proved backward by formula treatment （i.e. “Inferring syndromes with formulas”） according to the related theory of formula and syndrome，then the relationship between Ying-nutrients and Wei-defence and qi⁃blood are discussed. It is considered that Ying-nutrients and Wei-defence are derived from the middle jiao， and the upper jiao is pneumatized after the upper spleen is transfused into the heart and lung， and transformed into qi and blood， i.e. “The middle jiao receives qi， takes the juice and transforms into red liquid called blood”. Therefore， Ying-nutrients and Wei-defence are from the middle jiao， change in the upper jiao， with qi and blood homologous but different quality. In this sense， the essence of Ying-nutrients and Wei-defence is qi and blood.关键词：Huangqi Guizhi Wuwu Decoction;medicine group method;Ying-nutrients and Wei-defence;qi and blood;syndromes and treatments
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发布时间：2022-11-01 - 摘要：Syndrome differentiation and pathogenesis exploration is the key to achieve good effect in the clinic of traditional Chinese medicine （TCM）. Syndrome is a complex and multi-dimensional concept which is affected by many kinds of factors. It is quite consistent with the theory of complex giant system， so it is suitable to use the theory of complex giant system to study the speculative procedure of syndrome and pathogenesis. This paper conducts a comprehensive and in-depth conception exploration through discussing the theory， method and feasibility of using the complex giant system theory to study the speculative procedure of TCM syndrome differentiation and pathogenesis exploration. Through the investigations on the brief introduction of speculative procedure of TCM syndrome differentiation and pathogenesis exploration， the general situation of using modern scientific methods to study TCM syndrome differentiation， the design of using complex giant system to study the speculative procedure of TCM syndrome differentiation and pathogenesis exploration and its prospects， the rationality of the speculative procedure of syndrome and pathogenesis researched by complex giant system theory is analyzed. Using the theory of complex giant system to study the speculative procedure of TCM syndrome differentiation and pathogenesis exploration has feasibility and practical value for the research of TCM syndrome differentiation and pathogenesis theory.关键词：complex giant system;syndrome differentiation and pathogenesis exploration;speculative procedure;pathogenesis
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发布时间：2022-11-01
Theoretical Research
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