最新刊期
期 6 ， 2021
- 摘要：Objective:Based on the theory of property and flavor of Chines e medicine, the concept of "property and flavor network pharmacology" is proposed for the first time. The aim is to explore the mechanism of classical prescriptions Rujin Jiedu Powder in the treatment of viral pneumonia by constructing property and flavor network of Chinese medicine combined with molecular docking technology.Methods:The main components of Rujin Jiedu Powder were obtained from HERB database, and viral pneumonia related genes were collected by GeneCards. NetInfer and BATMAN-TCM databases were used to construct the relationship network of property and flavor module component-target-disease model for Rujin Jiedu Powder. The protein-protein interaction network and KEGG analysis for targets were performed by Metascape software. Molecular docking verification of components and target proteins were performed by Vina software. The pharmacokinetics of the active components was evaluated by SwissADME software.Results:A total of 335 active components of this prescription were screened out using Chinese medicine databases, among 20 components from Platycodonis Radix, 117 components from Glycyrrhizae Radix et Rhizoma, 26 components from Coptidis Rhizoma, 62 components from Scutellariae Radix, 75 components from Phellodendri Chinensis Cortex and 35 components from Gardenia Fructus. Target prediction and network construction were carried out according to three kinds of property-flavor modules, and there were 596 potential targets in bitter-pungent-calm module(Platycodonis Radix), 819 potential targets in sweet-calm module(Glycyrrhizae Radix et Rhizoma) and 1 097 potential targets in bitter-cold module(Coptidis Rhizoma, Scutellariae Radix, Phellodendri Chinensis Cortex, Gardenia Fructus) . Network analysis and molecular docking results showed the important active components(Isolicoflavonol, Clionasterol, Rutaecarpine, Licocoumarone, Baicalein, Quercetin and Luteolin), core target proteins(SRC, FYN, LCK, MET, AKT1, ABL1, MAPK8, ITK, CCR5, TP53, PTPN11, EIF2AK2, MAPK1, PIK3CD, MAPK3, BTK, STAT3, PIK3CA, MAPK14) and key signal pathways(JAK-STAT signaling pathway, cytokine-cytokine receptor interaction) of Rujin Jiedu Powder in the treatment of viral pneumonia.Conclusion:In this study, the property and flavor network pharmacology and molecular docking are used to reveal the characteristics of multi-components, multi-targets and multi-pathways of Rujin Jiedu Powder, which provides new idea and method for further studying the mechanism of Rujin Jiedu Powder in the treatment of viral pneumonia. Research also shows that, it is reasonable and feasible to explore the material basis of Chinese medicine compound recipes by property and flavor module component-target-disease model.关键词：property and flavor network pharmacology;Rujin Jiedu Powder;viral pneumonia;molecular docking
- 9
- |
- 1062
- |
- 0
发布时间：2022-09-09
Scholar Forum
- 摘要：Objective:To observe the clinical efficacy of Chuanhuang Formula combined with prostaglandin E1 regimen in treating patients of chronic kidney disease(CKD) complicated with acute kidney injury(AKI), and explore its therapeutic mechanisms from the perspective of Nod-like receptor protein 3(NLRP3) inflammasome.Methods:A total of 103 patients of CKD complicated with AKI(A on C) who met the inclusion criteria were randomly divided into the treatment group(52 cases) and control group(51 cases) .All patients were treated with basic therapy of western medicine.The patients in the control group were intravenously treated with prostaglandin E1 injection based on the basic treatment, and the patients in the treatment group were treated with Chuanhuang Formula by oral administration and coloclysis based on the treatment for the control group. The treatment course of both groups was 2 weeks.The clinical efficacy and the score of Chinese medical syndrome in both groups were evaluated. Before and after treatment, the levels of serum creatinine(Scr), blood urea nitrogen(BUN), blood uric acid(UA), estimated glomerular filtration rate(eGFR), 24-hour urinary protein(24 h UPro) and serum NLRP3, interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) and high sensitive C-reactive protein(hs-CRP) were detected.Results:①Clinical efficacy: After treatment, the total clinical effective rates of the treatment group and the control group were 73.1% and 33.3% respectively, and the efficacy of the treatment group was better than that of the control group(P<0.01) .②Chinese medical syndrome score: After treatment, the total score of Chinese medical syndrome in both groups was significantly decreased compared with that before treatment(P<0.01), and the total score of the treatment group was lower than that of the control group(P<0.05) .③Indexes of renal function: After treatment, the levels of Scr and UA in both groups were decreased compared with those before treatment(P<0.05, P<0.01), the level of eGFR was increased(P<0.05, P<0.01), the level of BUN in the treatment group was also decreased compared with that before treatment(P<0.01), and the levels of Scr, BUN and UA in the treatment group were lower than those in the control group(P<0.05), the level of eGFR in the treatment group was higher than that in the control group(P<0.05) .④24 h UPro: After treatment, the level of 24 h UPro in the treatment group was significantly decreased compared with that before treatment(P<0.01), and was significantly lower than that in the control group(P<0.05) .⑤Inflammatory indexes: After treatment, the levels of IL-6, TNF-α and hs-CRP in both groups were decreased compared with those before treatment(P<0.05, P<0.01), the level of NLRP3 in the treatment group was also decreased compared with that before treatment(P<0.01), and the levels of IL-6, TNF-α and NLRP3 in the treatment group were lower than those in the control group(P<0.05) .Conclusion:Compared with prostaglandin E1 alone, Chuanhuang Formula combined with prostaglandin E1 can better alleviate the kidney injury in patients of A on C, and its therapeutic mechanism may be related to the regulation of inflammatory status.关键词：chronic kidney disease;acute kidney injury;Chuanhuang Formula;prostaglandin E1;NLRP3 inflammasome
- 7
- |
- 341
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:To observe the clinical efficacy of Xiaobai Decoction combined with conventional western medicine in the treatment of chronic glomerulonephritis(CGN) with syndrome of spleen and kidney qi deficiency and stagnant dampness-heat.Methods:A total of 120 CGN patients[chronic kidney disease(CKD) at stages 1-2] with syndrome of spleen and kidney qi deficiency and stagnant dampness-heat were included and randomly divided into the control group and treatment group, 60 cases in each group. The patients in the control group were treated with the open scheme of western medicine, and the patients in the treatment group were orally treated with Xiaobai Decoction based on above.The treatment course of both groups was 6 months.The clinical efficacy and Chinese medical syndrome efficacy of the two groups were evaluated.Before and after treatment, the levels of 24-hour urinary protein quantity(24 h UPro), ratio of urinary microalbumin to urinary creatinine(UACR), urinary N-acetyl-β-D-glucosidase(NAG), urinary retinol binding protein(RBP), urinary α1-microglobulin(α1-MG), urinary β2-microglobulin(β2-MG), serum creatinine(Scr), blood urea nitrogen(BUN) and estimated glomerular filtration rate(eGFR) were detected.Results:During the study, 4 patients in the treatment group were removed or shed off and 5 patients in the control group were removed or shed off, finally 56 patients in the treatment group and 55 patients in the control group were included for the statistical analysis.①Clinical efficacy: After treatment, the total clinical effective rate was 92.9% in the treatment group and 87.3% in the control group, and the efficacy of the treatment group was better than that of the control group(P<0.05) .②Chinese medical syndrome efficacy: After treatment, the total effective rate on Chinese medical syndrome was 89.3% in the treatment group and 54.5% in the control group, and the efficacy of the treatment group was better than that of the control group(P<0.01) .③Laboratory indexes: After treatment, the levels of 24 h UPro, UACR, NAG and RBP in both groups were significantly decreased compared with those before treatment(P<0.05), the levels of α1-MG and β2-MG in the treatment group were also significantly decreased(P<0.05), and the levels of 24 h UPro, NAG, RBP, α1-MG and β2-MG in the treatment group were lower than those in the control group(P<0.05) .After treatment, the level of eGFR in the treatment group was significantly increased compared with that before treatment(P<0.05), the level of Scr was significantly decreased compared with that before treatment(P<0.05), and the level of Scr in the treatment group was lower than that in the control group(P<0.05) .④Correlation analysis: The eGFR was negatively correlated with 24 h UPro, UACR, NAG and RBP.Conclusion:Xiaobai Decoction combined with conventional western medicine can effectively improve the clinical symptoms, reduce the level of urinary protein, improve the related indexes of renal tubular injury and delay the progress of renal function in treating CGN patients(CKD at stages 1-2) with syndrome of spleen and kidney qi deficiency and stagnant dampness-heat.关键词：chronic glomerulonephritis;Xiaobai Decoction;syndrome of spleen and kidney qi deficiency and stagnant dampness-heat;proteinuria;integrated traditional Chinese and western medicine
- 13
- |
- 275
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:To investigate the effects of Jianpi Bushen Formula on intestinal flora in postoperative patients of colorectal cancer(CRC) with spleen-kidney yang deficiency syndrome.Methods:Forty postoperative patients of CRC with spleen-kidney yang deficiency syndrome and 10 healthy volunteers were included.After enrollment, the patients were orally treated with Jianpi Bushen Formula with a course of 14 days.Before and after intervention of Jianpi Bushen Formula, the relevant clinical symptom scores of all patients were evaluated and recorded, and the total symptom scores of the patients were compared.The feces specimens of the healthy volunteers and the patients before and after drug treatment were collected, the intestinal flora in all samples were detected by 16SrDNA sequencing technology, the differences of intestinal flora structure between the patients and the healthy volunteers were analyzed, and the intestinal flora structure of the patients before and after intervention of Jianpi Bushen Formula were compared.Results:①After intervention of Jianpi Bushen Formula, the total symptom score of CRC postoperative patients with spleen-kidney yang deficiency syndrome were significantly decreased compared with that before intervention(P<0.05) .②There were differences in the intestinal flora structure at all classification levels between the patients and the healthy volunteers.Before and after intervention of Jianpi Bushen Formula, the intestinal flora structure of the patients changed at all classification levels.Conclusion:There are differences in the intestinal flora structure between the healthy people and the CRC postoperative patients with spleen-kidney yang deficiency syndrome.The intervention of Jianpi Bushen Formula can improve the relevant clinical symptoms and the intestinal flora structure of the patients.The mechanism of Jianpi Bushen Formula improving the relevant symptoms of CRC postoperative patients with spleen-kidney yang deficiency syndrome may be related to improving the intestinal microecological environment.关键词：colorectal cancer;postoperation;spleen-kidney yang deficiency syndrome;Jianpi Bushen Formula;intestinal flora
- 12
- |
- 402
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:To observe the effects of Shiquan Dabu Decoction on metastatic biomarkers in breast cancer patients with syndrome of qi and blood deficiency during perioperative period.Methods:A randomized double-blinded controlled study was performed.A total of 81 breast cancer patients undergoing modified radical mastectomy were enrolled, and randomly divided into the treatment group(41 cases) and control group(40 cases) .All patients were treated with modified radical mastectomy for breast cancer, and treated with conventional western medicine after operation.The patients in the treatment group were orally treated with Shiquan Dabu Decoction granules from the 3rd day before operation, and the patients in the control group were orally treated with placebo granules.The granules were only stopped on the day of operation, and the treatment course of both groups was 14 days.The total score of Chinese medical syndrome and the efficacy of Chinese medical syndrome were compared between the two groups.On the 3rd day before operation and the 3rd and 7th days after operation, the serum levels of interleukin(IL) -6, IL-8, IL-10 and C-reactive protein(CRP) were detected, and the percentages of CD3+, CD8+T lymphocytes and natural killer cells(NK cells) were detected.On the 1st, 4th and 7th days after operation, the mass of surgical incision drainage fluid was measured and compared between the two groups.Results:During the study, one patient in the treatment group shed off, finally 40 patients in the treatment group and 40 patients in the control group were included for the statistical analysis.①Chinese medical syndrome: On the 7th day after operation, the total score of Chinese medical syndrome in the treatment group was significantly decreased compared with that on the 1st day after operation(P<0.05), and the total score of the treatment group was lower than that of the control group(P<0.05) .On the 7th day after operation, the total effective rate on Chinese medical syndrome was 52.5% in the treatment group and 22.5% in the control group, and the efficacy of the treatment group was better than that of the control group(P<0.05) .②Immune indexes: On the 3rd and 7th days after operation, the percentages of CD3+T lymphocytes and NK cells in the treatment group were significantly increased compared with those before operation(P<0.05), and the percentages of CD3+T lymphocytes and NK cells in the treatment group were higher than those in the control group(P<0.05) .③Inflammatory indicators: On the 3rd and 7th days after operation, the level of IL-6 in the treatment group was significantly decreased compared with that before operation(P<0.05) .On the 7th day after operation, the level of IL-6 in the control group was also significantly decreased compared with that before operation(P<0.05) .And on the 3rd and 7th days after operation, the level of IL-6 in the treatment group was lower than that in the control group(P<0.05) .On the 7th day after operation, the level of IL-8 in the treatment group was significantly decreased compared with that before operation(P<0.05), and was significantly lower than that in the control group(P<0.05) .On the 3rd and 7th days after operation, the level of CRP in both groups was significantly increased compared with that before operation(P<0.05) ; while on the 7th day after operation, the level of CRP in the treatment group was significantly lower than that in the control group(P<0.05) .④The mass of postoperative drainage fluid: On the 4th and 7th days after operation, the mass of surgical incision drainage fluid in the treatment group was significantly less than that in the control group(P<0.05) .Conclusion:The intervention of Shiquan Dabu Decoction can effectively improve the clinical syndrome, inhibit the postoperative inflammatory response and angiogenesis, and enhance the immune function of breast cancer patients with syndrome of qi and blood deficiency during perioperative period.关键词：breast cancer;perioperative period;syndrome of qi and blood deficiency;Shiquan Dabu Decoction;metastatic biomarker;double-blinded randomized controlled trial
- 16
- |
- 294
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:To observe the clinical efficacy of Qingyu Wentong Formula in treating patients with dysmenorrhea due to adenomyosis and its influence on the related inflammatory factors.Methods:A total of 62 patients with dysmenorrhea due to adenomyosis were included and divided into the treatment group(32 cases) and control group(30 cases) by random number table.The patients in the treatment group were orally treated with Qingyu Wentong Formula, and the patients in the control group were orally treated with Sanjie Zhentong Capsule.The treatment course was 3 menstrual cycles.The improvement efficacy on dysmenorrhea in both groups was evaluated and compared, the pain degree of dysmenorrhea was evaluated by visual analogue scale(VAS), and the serum levels of carbohydrate antigen 125(CA125), high sensitivity C-reactive protein(hs-CRP), interleukin-6(IL-6) and monocyte chemoattractant protein-1(MCP-1) were detected.Results:①After treatment, the total effective rate of dysmenorrhea improvement was 84.38% in the treatment group and 60.00% in the control group, and the efficacy of the treatment group was better than that of the control group(P<0.05) .②After treatment, the VAS score of dysmenorrhea pain in both groups was significantl decreased compared with that before treatment(P<0.01), but there was no statistically significant difference on the score between the two groups(P>0.05) .③After treatment, the levels of hs-CRP, IL-6 and MCP-1 in both groups were decreased compared with those before treatment(P<0.05), the level of CA125 in the treatment group was also decreased compared with that before treatment(P<0.05), and the levels of CA125, hs-CRP, IL-6 and MCP-1 in the treatment group were lower than those in the control group(P<0.05) .Conclusion:Qingyu Wentong Formula can significantly reduce the symptoms of dysmenorrhea in patients with adenomyosis, and its mechanisms may be related to inhibiting the production of serum proinflammatory cytokines, reducing inflammatory reaction and improving the micro-inflammatory status of uterine environment.关键词：adenomyosis;dysmenorrhea;Qingyu Wentong Formula;inflammatory factor
- 14
- |
- 434
- |
- 0
发布时间：2022-09-09
Clinical Research
- 摘要：Objective:To explore the mechanism of Jianpi Jiedu Recipe regulating histone demethylase JMJD2C against colorectal cancer metastasis.Methods:①Twenty-four nude mice were taken and injected with colorectal cancer HCT116 cells via caudal vein, to replicate the lung metastasis model of colorectal cancer.Seven days after modeling, the nude mice were equally divided into four groups, and treated with Jianpi Jiedu Recipe decoction at different doses(0, 250, 500, 1 000 mg/kg) by intragastric administration.After drug treatment for 28days, the lung metastasis tissuewas isolated, andtheexpressionofJMJD2Cproteinwas detectedby immunohistochemistry.②HCT116 and LoVo cells were taken as the research objects, respectively.The cells were treated with alcohol extract of Jianpi Jiedu Recipe at different concentrations(0, 50, 100, 200 μg/ml) .After drug treatment for 48 hours, the expression of JMJD2C mRNA was detected by PCR, the expression of JMJD2C protein was detected by Western blot, and the protein expression and cell localization of JMJD2C were detected by immunofluorescence.Results:①Jianpi Jiedu Recipe could significantly down-regulate the expression of JMJD2C protein in lung metastases of colorectal cancer in nude mice, and the inhibitory effect showed in a dose-dependent manner.②Jianpi Jiedu Recipe could significantly down-regulate the mRNA and protein expressions of JMJD2C in these two colorectal cancer cells, and Jianpi Jiedu Recipe at 100 and 200 μg/ml showed more significant effects.③Jianpi Jiedu Recipe could inhibit the accumulation of JMJD2C protein in the nucleus of colorectal cancer cells, and Jianpi Jiedu Recipe at 100 and 200 μg/ml showed more significant effects.Conclusion:Jianpi Jiedu Recipe can inhibit the metastasis of colorectal cancer, and the mechanism may be related to the down-regulation of JMJD2C expression and its accumulation in the nucleus.关键词：Jianpi Jiedu Recipe;histone demethylase JMJD2C;colorectal cancer;metastasis;nude mouse
- 14
- |
- 318
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:To investigate the effects of dihydroartemisinin(DHA) on the proliferation and apoptosis of human triple-negative breast cancer MDA-MB-231 cells based on PI3K/AKT signaling pathway.Methods:①After treatment with DHA at different concentrations(25, 50, 100 μmol/L) for different times(12, 24, 48, 72 h), the cell proliferation was detected by MTT assay.②After treatment with DHA at different concentrations(0, 30, 60, 90 μmol/L) for 48 hours, the cell apoptosis and cell cycle were detected by flow cytometry, and the cell apoptosis was observed by Hoechst 33258 staining.③The effect of 60 μmol/L DHA on the cell migration was observed by scratch test after treatment for 0, 6, 12 and 24 hours respectively.④After treatment with DHA at 60 μmol/L for 12 hours, the mRNA expression levels of apoptosis related factors Caspase-3, Caspase-9, Bcl-2 and Bax were detected by PCR.After treatment with DHA at 60 μmol/L for 48 hours, the protein expression levels of Caspase-3, Caspase-9, Bcl-2, Bax, PI3K, p-PI3K, AKT and p-AKT were detected by Western blot.Results:①DHA could inhibit the proliferation of MDA-MB-231 cells, and the inhibitory effect was increased with the increase of drug concentration.②DHA could promote the apoptosis of MDA-MB-231 cells, and this effect was increased with the increase of drug concentration.③DHA at 30 μmol/L could block the cell cycle in G0/G1 phase, and DHA at 60 and 90 μmol/L could block the cell cycle in G2/M phase.④After treatment with DHA at 60 μmol/L for 12 and 24 hours, the cell migration rate was decreased significantly(P<0.01) .⑤After treatment with DHA at 60 μmol/L, the expression levels of Caspase-3 and Caspase-9 mRNA and the expression ratio of Bax/Bcl-2 mRNA were significantly up-regulated(P<0.01), and the expression ratios of cleaved Caspase-9/Caspase-9 and Bax/Bcl-2 protein were significantly increased(P<0.05) ; meanwhile, the protein expression level of PI3K was increased significantly(P<0.05), and the protein expression levels of p-PI3K and p-AKT were decreased significantly(P<0.01) .Conclusion:DHA may inhibit the proliferation and migration of MDA-MB-231 cells and induce the cell apoptosis by inhibiting PI3K/AKT signaling pathway.关键词：dihydroartemisinin;triple-negative breast cancer;cell proliferation;cell apoptosis;PI3K/AKT signaling pathway;MDA-MB-231 cell
- 11
- |
- 631
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:To investigate the therapeutic effects of Huganning Tablets on high fat diet induced non-alcoholic fatty liver disease(NAFLD) in mice, as well as its influence on the intestinal mucosal mechanical barrier function.Methods:Male C57BL/6J mice were randomly divided into the normal group, model group and Huganning Tablets(0.6 g·kg-1·d-1) group, 8 mice in each group.Except the normal group, the other mice were fed with high fat diet for 12 weeks to induce the NAFLD model.After modeling, the mice in each group were treated with the corresponding drug by intragastric administration for 4 weeks.After the last administration, the serum was taken and the colon and liver tissues were collected.The serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total cholesterol(TC), triglyceride(TG), low-density lipoprotein(LDL) and high-density lipoprotein(HDL) were detected by biochemical analyzer.The morphological changes of colon and liver tissues were observed after HE staining, and the lipid accumulation in liver tissue was observed after oil red O staining.The levels of TG and TC in liver tissue were detected by kits.The mRNA expressions of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β), interleukin-6(IL-6), zonula occludens-1(ZO-1) 、Claudin-1 and Occludin were detected by PCR.The protein expressions of ZO-1, Claudin-1 and Occludin were detected by Western blot.Results:①The serum levels of ALT, AST, TG, TC, HDL and LDL in the model group were significantly higher than those in the normal group(P<0.01) .Huganning Tablets could significantly decrease the serum levels of ALT, AST, TC, HDL and LDL in model mice(P<0.05) .②Huganning Tablets could significantly reduce the TG level in the liver of model mice(P<0.01), and obviously improve the hepatocyte steatosis and lipid accumulation.③The colon tissue of mice in the model group showed obvious crypt structure destruction, epithelial cell injury and inflammatory cell infiltration, and the mRNA expression levels of TNF-α, IL-1β and IL-6 were significantly higher than those in the normal group(P<0.05, P<0.01) .Huganning Tablets could significantly improve the pathological injury of colon tissue in model mice, and significantly reduce the mRNA expression levels of TNF-α and IL-6(P<0.05) .④The mRNA expression levels of ZO-1 and Claudin-1 in colon tissue of mice in the model group were significantly lower than those in the normal group(P<0.05, P<0.01), and the protein expression levels of ZO-1, Claudin-1 and Occludin were significantly lower than those in the normal group(P<0.05) .Huganning Tablets could significantly increase the mRNA expression levels of ZO-1, Claudin-1 and Occludin in colon tissue of model mice(P<0.05), and up-regulate the protein expression levels of ZO-1 and Occludin(P<0.05) .Conclusion:Huganning Tablets can improve the liver function and blood lipid metabolism of NAFLD model mice, and reduce the injury of liver and colon tissues.Its mechanism may be related to up-regulating the expressions of tight junction proteins ZO-1, Claudin-1 and Occludin in colon tissue and improving the intestinal mucosal mechanical barrier function.关键词：Huganning Tablets;NAFLD;intestinal mucosal mechanical barrier;tight junction protein;mouse
- 21
- |
- 271
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:To optimize the preparation process of thermosensitive 20(S) -Protopanaxadiol(PPD) microemulsion-gel composite system, and investigate its gelation temperature, gelation time, appearance characteristics, rheology, and drug release in vitro and mechanism.Methods:Based on the preparation process of PPD microemulsion in the previous stage, the thermosensitive PPD microemulsion-gel composite system was prepared by cold-dissolving method.The influence of different excipients on the gelation temperature was investigated by single factor test to determine the basic formula, including poloxamer 407(P407), hydroxypropyl methyl cellulose(HPMC), sodium alginate(SA) and glycerin.Then, the optimal formulation and process of the composite system were selected according to the gelation temperature, gelation time and in vitro release.The morphology was observed by scanning electron microscope(SEM) and transmission electron microscope(TEM) .The rheological properties were detected by rotary rheometer.The drug release rate in vitro was determined by dialysis method, and the equations were fitted to explore the drug release mechanism.Results:The optimized formulation of thermosensitive PPD microemulsion-gel composite system was as follows: 30% P407 aqueous solution, 0.6% HPMC, 0.2% SA, 4% glycerol, 0.05% Nipagin ethyl ester, and the volume ratio of P407 aqueous solution to PPD microemulsion was 2∶1.For thermosensitive PPD microemulsion-gel prepared under this condition, the gelation temperature was(30.9±0.1) ℃and the gelation time was(146.7±4.4) s.SEM showed porous structure and loose pores, and TEM showed that the microemulsion particles were round and without adhesion.The preparation showed good thermosensitivity and good fluidity at room temperature.It could undergo phase transition from solution to gel when slight below skin temperature.The release time in vitro was 72 hours.The drug release process in vitro conformed to Weibull equation, which was the synergistic effect of drug diffusion and skeleton dissolution.Conclusion:This thermosensitive PPD microemulsion-gel composite system shows simple preparation process, suitable gelationtemperature and time, andexhibits thermosensitivity and sustained release, which can meet the local medication needs of the skin.关键词：20 (S) -Protopanaxadiol;microemulsion-gelcompositesystem;thermosensitivity;preparationprocess;drug release mechanism
- 5
- |
- 331
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:Twenty-one glycosyltransferase genes, cloned from Notoginseng Radix et Rhizoma in previous study, are separately constructed into pCold expression vector to realize the soluble expression of recombinant protein, and their catalytic function towards flavonoids in vitro is screened and identified.Methods:Phylogenetic tree analysis was performed using 21 glycosyltransferase genes.These genes were separately cloned into pCold expression vector by homologous recombination, and the recombinant proteins were expressed in Escherichia coli.The recombinant proteins were induced to express at low temperature.Taken flavonoids as glycosyl receptor substrates and UDP-glucose as glycosyl donor, the enzymatic reaction products were detected by high performance liquid chromatography(HPLC) .Results:Using kaempferol and apigenin as substrates respectively, kaempferol 7-O glucoside and apigenin 7-O glucoside could be detected after the recombinant protein PnUGT82 participated in the reaction; while the corresponding glycoside products were not detected in the negative control group.Conclusion:A glucosyltransferase PnUGT82 from Notoginseng Radix et Rhizoma is obtained, which can catalyze flavonoids.The glucosyl from UDP-glucose is transferred to the 7-hydroxyl of kaempferol or apigenin, to form the corresponding flavonoid 7-O glycoside products in vitro.关键词：Notoginseng Radix et Rhizoma;flavonoid;glycosyltransferase;recombinant protein;catalytic activity
- 7
- |
- 586
- |
- 0
发布时间：2022-09-09 - 摘要：Objective:To systematically study the environment, botanical origin, character, micro-character and microscopic identification characteristics of 48 batches of Lycium ruthenicum Murr.from 5 different areas, and provide the basis for the identification and quality control of Lycium ruthenicum Murr..Methods:The pharmacognosy of the original plants and medicinal materials of Lycium ruthenicum Murr.were systematically studied, using the botanical identification, character identification, micro-character identification, microscopic identification and comparative observation under normal light and polarized light, combined with real-time depth of field expansion technology, and the holographic color images were collected.Results:The "little skirt" -shaped corolla residues and large sclereids on the inner side of the upper epidermis of calyx were found for the first time, with important significance for identification.The image data of the characteristics of botanical origin, character and fine structure of Lycium ruthenicum Murr.were obtained, and the holographic color images of the seed transverse, the calyx transverse and the powder under normal light and polarized light were obtained.The Lycium ruthenicum Murr.planted in Ningxia and Qinghai showed the best overall quality.Conclusion:The obtained environment, botanical origin, character and microscopic identification characteristics may provide the basis for the pharmacognosy research of Lycium ruthenicum Murr..关键词：Lycium ruthenicum Murr.;pharmacognosy;microscopic identification;micro-character identification;polarized light;real-time depth of field expansion
- 12
- |
- 510
- |
- 0
发布时间：2022-09-09
Experiment Research
- 摘要：As traditional Chinese herbal medicine, Anemarrhenae Rhizoma shows the efficacy of nourishing yin for moistening dryness as well as clearing heat-fire, which is widely applied in clinic.Timosaponin B-Ⅱ(TB-Ⅱ) is one of the main active components of Anemarrhenae Rhizoma.Modern pharmacological studies have found that TB-Ⅱ shows many pharmacological activities, such as anti-Alzheimer’s disease, anti-depression, anti-diabetes and its complications, anti-osteoporosis, anti-platelet aggregation, anti-inflammation and anti-tumor.The research progress related to the pharmacological activity and mechanism of TB-Ⅱ in recent years are summarized, so as to provide reference for the new drug development and clinical application of TB-Ⅱ.关键词：timosaponin B-Ⅱ;pharmacological activity;mechanism;review
- 8
- |
- 1269
- |
- 0
发布时间：2022-09-09
Review
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
- Total Visits：10154 Visits Today：17
0