最新刊期
期 5 ， 2021
- 摘要：Network pharmacology has become a popular tool for the research on pharmacological basis and action mechanism of traditional Chinese medicine(TCM) .It is widely used in the screening of active components of Chinese medicines, repositioning of drugs, exploration of the mechanism of compatibility of Chinese medicines, and explanation of the action mechanism of multi-components, multi-targets and multi-pathways of Chinese medicines.The development stages of network pharmacology are reviewed, the databases related to network pharmacology at the present stage, such as TCM database, disease database and functional annotation database, are sorted out, and the application of network pharmacology in the research of TCM modernization is briefly summarized.It also points out the problems and challenges of network pharmacology research, and then looks forward to the development direction of TCM network pharmacology research.The purpose of this paper is to provide guidance for standardized and rational application of network pharmacology in TCM research, and provide ideas for leading the development of TCM original researches and the research and development of new Chinese medicines.关键词：network pharmacology;traditional Chinese medicine;database
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发布时间：2022-09-09
Scholar Forum
- 摘要：Objective:To investigate the effects of Shuxuetong Injection on coronary microcirculation and serum intercellular adhesion molecule-1(ICAM-1), tumor necrosis factor-α(TNF-α) and high sensitivity C reactive protein(hs-CRP) levels in patients with acute ST-segment elevation myocardial infarction(STEMI) after percutaneous coronary intervention(PCI) .Methods:A total of 64 patients with acute STEMI were included, and randomly divided into the control group and the Shuxuetong group, 32 cases in each group.All patients were treated with PCI, and the drug intervention was performed from the day of admission.The patients in the control group were treated with conventional western medicine, and the patients in the Shuxuetong group were treated with Shuxuetong Injection based on conventional western medicine, with a course of 7 days.Before operation(before treatment) and 7 days after operation(after treatment), the scores of Chinese medical syndrome were evaluated, and the efficacy of Chinese medical syndrome was compared between the two groups.Thirty minutes, 2 hours, 24 hours and 7 days after operation, the distribution difference of patients with complete ST-segment resolution(STR) in electrocardiogram was evaluated and compared between the two groups.Before and after treatment, the serum levels of ICAM-1, TNF-α and hs-CRP in the two groups were detected and compared.Results:During the study, two patients in the control group were eliminated.Finally 32 cases in the Shuxuetong group and 30 cases in the control group were included for the statistical analysis.①After treatment, the scores of the main and secondary symptoms of Chinese medical syndrome were significantly decreased in both groups compared with those before treatment(P<0.01) .However, there were no statistically significant differences on the scores between the two groups(P>0.05) .After treatment, the total effective rate of Chinese medical syndrome was 93.75% in the Shuxuetong group and 90.00% in the control group respectively, and the efficacy of the Shuxuetong group was better than that of the control group(P<0.05) .②Seven days after operation, the proportion of patients with complete STR in electrocardiogram in the Shuxuetong group was significantly increased compared with that in the control group(P<0.05) .③After treatment, the ICAM-1 level was increased in both groups compared with that before treatment(P<0.05, P<0.01), and the levels of TNF-α and hs-CRP were decreased compared with those before treatment(P<0.05, P<0.01) .The levels of ICAM-1, TNF-α and hs-CRP in the Shuxuetong group were significantly lower than those in the control group(P<0.05, P<0.01) .Conclusion:Shuxuetong Injection combined with conventional western medicine can improve the clinical symptoms and the coronary microcirculation in treating patients with STEMI after PCI, slow down the increase of adhesion molecule level after myocardial infarction, and decrease the levels of inflammatory factors.关键词：acute ST-segment elevation myocardial infarction;PCI postoperation;Shuxuetong Injection;coronary microcirculation;intercellular adhesion factor-1
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发布时间：2022-09-09 - 摘要：Objective:To explore the clinical efficacy of Shuxin Oral Liquid combined with conventional western medicine in the treatment of stable coronary heart disease(SCHD) with anxiety based on "psycho-cardiology disease" theory in traditional Chinese medicine(TCM) .Methods:A total of 90 SCHD patients with anxiety were included and randomly divided into the treatment group and control group, 45 cases in each group.The patients in both groups were treated with conventional western medicine, meanwhile the patients in the treatment group were treated with Shuxin Oral Liquid.The treatment course was 12 weeks.Before treatment and 4 and 12 weeks after treatment, the frequency of angina attack and the nitroglycerin dosage were recorded, and the self-made TCM syndrome score scale, Hamilton anxiety(HAMA) scale and Seattle angina questionnaire(SAQ) were applied to evaluate the clinical symptoms, emotional state and quality of life.Results:During the study, 2 patients in the treatment group and 2 patients in the control group were eliminated or fell off.Finally 43 cases in the treatment group and 43 cases in the control group were included for the statistical analysis.①Four and twelve weeks after treatment, the frequency of angina attack and the nitroglycerin dosage were significantly reduced in both groups compared with those before treatment(P<0.05), and the frequency of angina attack and the nitroglycerin dosage in the treatment group were significantly less than those in the control group(P<0.05) .②Four and twelve weeks after treatment, the total score and cardiovascular system factor score of HAMA were significantly decreased in the treatment group compared with those before treatment(P<0.05), and the scores of the treatment group were obviously lower than those of the control group(P<0.05) .③Four and twelve weeks after treatment, the total score of TCM syndromes and the scores of chest pain, palpitation and short breath were significantly reduced in both groups compared with those before treatment(P<0.05), and the scores of the treatment group were lower than those of the control group(P<0.05) .④Four and twelve weeks after treatment, all dimension scores of SAQ were significantly increased in both groups compared with those before treatment(P<0.05), and all dimension scores of SAQ in the treatment group were significantly higher than those in the control group(P<0.05) .Conclusion:Based on the "psycho-cardiology disease" theory of TCM, Shuxin Oral Liquid combined with conventional western medicine can obviously improve the clinical symptoms and anxiety and enhance the quality of life in treating SCHD patients with anxiety.关键词：stable coronary heart disease;anxiety;psycho-cardiology disease;Shuxin Oral Liquid;integrated traditional Chinese and western medicine
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发布时间：2022-09-09 - 摘要：Objective:To observe the effects of Runzao Yishen Decoction on glucose metabolism, blood glucose excursion and Chinese medical syndrome score in patients of type 2 diabetes mellitus(T2DM) with syndrome of kidney yin deficiency based on continuous glucose monitoring.Methods:A total of ninety-eight T2DM patients with syndrome of kidney yin deficiency were randomly divided into the treatment group and control group, 49 cases in each group.The control group was treated with insulin, and the treatment group was treated with Runzao Yishen Decoction on the basis of the control group, with a course of 12 weeks.The glycosylated hemoglobin(HbA1c), fasting plasma glucose(FPG), 2-hour postprandial glucose(2hPG), serum creatinine(Scr), alanine aminotransferase(ALT), fasting C-peptide(FCP), 2-hour postprandial C-peptide(2hCP), glycosylated albumin(GA), continuous glucose monitoring parameters, Chinese medical syndrome score and insulin dosage were compared between the two groups before and after treatment.Results:①After treatment, the levels of HbA1c, FPG and 2hPG were decreased significantly in both groups(P<0.05), and the decrease in the treatment group was more obvious than that in the control group(P<0.05) .②After treatment, calculated by continuous glucose monitoring system, the standard deviations(SD) of blood glucose, mean amplitude of glycemic excursion(MAGE) and mean blood glucose(MBG) were significantly decreased in both groups(P<0.05), and the time in range(TIR) was significantly increased(P<0.05), and the increase of TIR in the treatment group was significantly higher than that in the control group(P<0.05) .Taken TIR>70% as the standard point, the rate of TIR compliance in the treatment group was 75.51%, which was significantly higher than that in the control group(55.10%) (P<0.05) .③After treatment, the total score of Chinese medical syndrome in both groups was decreased, and the score in the treatment group was lower than that in the control group(P<0.05) .④The difference of daily insulin dosage between pre- and post-therapy in the treatment group was lower than that in the control group(P<0.05) .Conclusion:Runzao Yishen Decoction can reduce the blood glucose level and glucose excursion, significantly increase TIR and improve Chinese medical syndrome in T2DM patients.关键词：type 2 diabetes mellitus;Runzao Yishen Decoction;kidney yin deficiency;continuous glucose monitoring;glucose fluctuation
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发布时间：2022-09-09 - 摘要：Objective:To analyze the traditional Chinese medicine(TCM) syndrome differentiation and western medicine clinical classification of patients with Parkinson’s disease(PD), and explore the clinical characteristics of PD patients in TCM and western medicine.Methods:A total of 209 patients with early and middle stage PD were included, the TCM syndrome differentiation and western medicine clinical classification of these patients were analyzed, and the motor symptoms, non-motor symptoms and quality of life were evaluated by the related scales.Meanwhile, the correlation between TCM syndrome differentiation and western medicine clinical characteristics was analyzed by multiple correspondence analysis.Results:According to the TCM syndrome differentiation, patients with syndrome of liver and kidney deficiency were the most, followed by syndrome of qi and blood deficiency and syndrome of qi stagnation and blood stasis, and patients with syndrome of phlegm heat and wind stirring were the least.According to the western medicine classification, patients with postural instability/gait disorder(PIGD) type were the most, followed by tremor dominate(TD) type, and patients with intermediate type were the least.The results of multiple correspondence analysis showed that, the age of PD patients with syndrome of qi stagnation and blood stasis was mostly not more than 55 years old, these patients were mainly female with mild motor symptoms and common unilateral limb involvement in clinic.The age of PD patients with syndrome of liver and kidney deficiency was mostly more than 65 years old, the clinical manifestations of these patients included postural and gait disorders, with slightly severe motor symptoms and common bilateral limb involvement.The patients with above two syndromes showed less non-motor symptoms and better quality of life.The life quality of PD patients with syndrome of phlegm heat and wind stirring and syndrome of qi and blood deficiency was slightly worse, which may be related to more and serious non-motor symptoms in patients with syndrome of phlegm heat and wind stirring and serious motor symptoms in patients with syndrome of qi and blood deficiency.Conclusion:The syndrome of liver and kidney deficiency is the most common in early and middle stage PD patients, and the clinical manifestations of these patients are postural instability/gait disorder.It is necessary to combine TCM syndrome differentiation with western medicine classification in the treatment, so as to enhance the clinical efficacy.关键词：Parkinson’s disease;clinical classification;Chinese medical syndrome differentiation and classification
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发布时间：2022-09-09 - 摘要：Objective:To evaluate the clinical efficacy of tube-dragging therapy with multiple incisions in the treatment of high horseshoe anal fistula.Methods:A total of 72 patients with high horseshoe anal fistula were included and randomly divided into the treatment group and control group, 36 cases in each group.The patients in the treatment group were treated with tube-dragging therapy with multiple incisions, and the patients in the control group were treated with traditional incision and thread-drawing therapy.The wound healing time of the two groups was compared.On the 3rd, 7th and 14th days after operation, the scores of wound pain, effusion and edema in the two groups were evaluated.Three months after operation, the scores of anal function and incontinence, and the clinical efficacy were evaluated and compared.Results:In the course of study, one patient in the control group fell off, finally 36 cases in the treatment group and 35 cases in the control group were included for the statistical analysis.①Three months after operation, the total clinical effective rate was 97.2% in the treatment group and 94.3% in the control group, with no statistically significant difference between the two groups(P>0.05) .②The wound healing time in the treatment group was significantly shorter than that in the control group(P<0.05) .③On the 7th and 14th days after operation, the scores of wound pain and effusion in the treatment group were lower than those in the control group(P<0.05, P<0.01) .After operation, there was no statistically significant difference in the score of wound edema between the two groups(P>0.05) .④Three months after operation, there was no statistically significant difference in the score of anal function between the two groups(P>0.05), and the score of anal incontinence in the treatment group was significantly lower than that in the control group(P<0.05) .Conclusion:Compared with traditional incision and thread-drawing therapy, tube-dragging therapy with multiple incisions can reduce the postoperative complication, shorten the healing time and protect the anal sphincter function in the treatment of high horseshoe anal fistula, which is worth popularizing and applying in clinic.关键词：high horseshoe anal fistula;tube-dragging therapy with multiple incisions;incision and thread-drawing therapy
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发布时间：2022-09-09
Clinical Research
- 摘要：Objective:To investigate the therapeutic effects and potential mechanisms of Dangfei Liganning Capsule on high fat diet induced non-alcoholic fatty liver disease(NAFLD) in mice.Methods:Male C57BL/6J mice were randomly divided into the normal group, model group and Dangfei Liganning(0.42 g·kg-1·d-1) group, 10 mice in each group.Except the normal group, mice in the other groups were fed with high fat diet for 12 weeks to induce the model.After modeling, each group was treated with the corresponding drug by intragastric administration for 4 weeks.After the last administration, the serum was taken and the liver tissue was collected.The serum levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total cholesterol(TC), triglyceride(TG), low-density lipoprotein(LDL) and high-density lipoprotein(HDL) were detected by biochemical analyzer.The morphological changes of liver tissue were observed after oil red O staining and HE staining.The levels of TC, TG, malondialdehyde(MDA), glutathione(GSH) and superoxide dismutase(SOD) in liver tissue were detected by kits.The mRNA expressions of nuclear factor erythroid 2-related factor 2(Nrf2), cytochrome P450 2E1(CYP2E1), heme oxygenase 1(HO-1), NADPH quinone oxidoreductase 1(NQO1) and glutathione S-transferase P1(GSTP1) in liver tissue were detected by PCR.The protein expressions of Nrf2 and CYP2E1 in liver tissue were detected by Western blot.Results:①Compared with the model group, the serum levels of ALT, AST, TC and LDL were significantly decreased in the Dangfei Liganning group(P<0.05) .②Dangfei Liganning Capsule could significantly reduce the TG level in the liver of model mice(P<0.05), and significantly alleviate the hepatocyte steatosis and lipid accumulation in the model mice.Compared with the model group, the activity of SOD in the liver was increased obviously in the Dangfei Liganning group(P<0.05), and the content of MDA in the liver was significantly decreased(P<0.05) .③The mRNA expression levels of Nrf2, HO-1, GSTP1, NQO1 and CYP2E1 in the model group were significantly lower than those in the normal group(P<0.05, P<0.01) .Dangfei Liganning Capsule could significantly up-regulate the mRNA expression levels of Nrf2, GSTP1 and NQO1 in the liver of model mice(P<0.05) .④The protein expression levels of Nrf2 and CYP2E1 in the model group were significantly lower than those in the normal group(P<0.05, P<0.01) .Dangfei Liganning Capsule could significantly increase the protein expression levels of Nrf2 and CYP2E1 in the liver of model mice(P<0.05) .Conclusion:Dangfei Liganning Capsule shows good therapeutic effects on NAFLD mice induced by high fat diet, and its mechanism is related to the decrease of Nrf2 mediated oxidative stress in liver.关键词：Dangfei Liganning Capsule;NAFLD;oxidative stress;Nrf2;mouse
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发布时间：2022-09-09 - 摘要：Objective:To observe the aristolochic acid Ⅰ(AA Ⅰ) -induced liver injury in rats and investigate its influence on autophagy-related factors.Methods:Male Wistar rats were randomly divided into the normal group, autophagy inhibitor group, AA Ⅰ low-dose(20 mg/kg) group, AA Ⅰ high-dose(40 mg/kg) group, autophagy inhibitor + AA Ⅰ low-dose group and autophagy inhibitor + AA Ⅰ high-dose group, five rats in each group.Rats in the autophagy inhibitor treatment groups were intraperitoneally injected with 3-methyladenine(3-MA, 20 mg/kg) in advance.One hour after the inhibitor intervention, the AA Ⅰ treatment groups were intraperitoneally injected with AA Ⅰ at the corresponding dose.Drug intervention lasted for 9 days.After the last administration, blood was taken and liver was isolated.The serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were detected by biochemical analyzer, and the ratio was calculated.The morphological changes of liver tissue were observed under light and transmission electron microscope.The mRNA expression levels of Beclin 1, LC3 and p53 were detected by PCR.The protein expression levels of Beclin 1, LC3 and p53 were detected by immunohistochemistry.Results:①Compared with the normal group, the serum AST level in the AA Ⅰ low-dose group was increased significantly(P<0.05), and the AST/ALT ratio was obviously increased in the AA Ⅰ low-dose and high-dose groups(P<0.05, P<0.01) .Compared with the autophagy inhibitor group, the AST/ALT ratio was increased significantly in the autophagy inhibitor + AAⅠ high-dose group and autophagy inhibitor + AA Ⅰ low-dose group(P<0.01) .② After HE staining, hepatocyte swelling was observed in the AA Ⅰ low-dose and high-dose groups, and the degree of injury was more significant in the AA Ⅰ high-dose group. Hepatocyte hemorrhagic necrosis was found in the autophagy inhibitor + AA Ⅰ high-dose group, and the degree of injury was significantly worse than that in the AA Ⅰ high-dose group. Transmission electron microscopy showed the mitochondria swelling in the AA Ⅰ low-dose and high-dose groups, and the steatosis was also observed in the AA Ⅰ high-dose group.Mitochondrion swelling was found in the autophagy inhibitor + AA Ⅰ low-dose group, as well as the endoplasmic reticulum swelling and implicit autophagy bodies in the autophagy inhibitor + AA Ⅰ high-dose group.③Compared with the normal group, the expression of p53 mRNA was significantly up-regulated in the AA Ⅰ low-dose and high-dose groups(P<0.05) .Compared with the autophagy inhibitor group, the expression of LC3 mRNA was significantly decreased in the autophagy inhibitor + AA Ⅰ low-dose group(P<0.05), and the expressions of Beclin 1 and LC3 mRNA were significantly decreased in the autophagy inhibitor + AA Ⅰ high-dose group(P<0.01) .The mRNA expressions of Beclin 1, LC3 and p53 in the autophagy inhibitor + AA Ⅰ high-dose group were significantly lower than those in the autophagy inhibitor + AA Ⅰ low-dose group(P<0.05, P<0.01) .④Compared with the normal group, the protein expressions of Beclin 1, LC3 and p53 were significantly up-regulated in the AA Ⅰ low-dose and high-dose groups(P<0.01), and the expressions of these three proteins in the AA Ⅰ high-dose group were significantly higher than those in the AA Ⅰ low-dose group(P<0.05, P<0.01) .Compared with the autophagy inhibitor group, after the co-treatment of autophagy inhibitor and AA Ⅰ with different doses, the expressions of these three proteins were significantly up-regulated(P<0.01) .Compared with the autophagy inhibitor + AA Ⅰ low-dose group, the expressions of Beclin 1 and LC3 proteins were decreased significantly(P<0.05, P<0.01) and the expression of p53 protein was increased significantly(P<0.01) in the autophagy inhibitor + AA Ⅰ high-dose group.Conclusion:AA Ⅰ can induce liver injury in rats, and autophagy factors promote AA Ⅰ liver injury.Its mechanism may be related to regulating p53 protein expression and inducing autophagy.关键词：aristolochic acid Ⅰ;liver injury;autophagy;rat
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发布时间：2022-09-09 - 摘要：Objective:To investigate the effects of Qingfei Paidu Decoction(QFPDD) on megakaryocyte differentiation and platelet production.Methods:Human megakaryocyte DAMI cell line was taken as the research object.The water decoction of QFPDD was prepared.①DAMI cells were divided into the QFPDD groups with different concentrations(0, 25, 50, 75 mg/L), and were treated with the decoction at corresponding concentration.After treatment for 48 hours, the cell proliferation was detected by CCK-8 assay, the DNA content was detected by flow cytometry, and the mRNA expression levels of transcription factors aNF-E2, fNF-E2, GATA-1 and Fli-1 were detected by PCR.②The cells were divided into the control group and QFPDD groups with different concentrations(25, 50, 75 mg/L) . All groups were treated with 1 μmol/L PMA and 10 ng/ml TPO for 7 days, to induce cell differentiation; meanwhile, QFPDD intervention groups were treated with the decoction at corresponding concentration.After intervention for 7 days, the cells were cultured for 3 days without drug and inducer stimulation, and then the cells and supernatant were collected.The DNA content and the number of platelet-like particles were detected by flow cytometry, and the morphology of megakaryocytes was analyzed.Results:①QFPDD at different concentrations showed no significant effect on the proliferation of undifferentiated DAMI cells.QFPDD at concentration of 50 mg/L and 75 mg/L could induce the undifferentiated DAMI cells into G2/M phase, which significantly increased the proportion of cells with DNA content of 4N(P<0.01) .②QFPDD treatment could significantly increase the DNA ploidy of differentiated DAMI cells.The proportion of cells with DNA ploidy≥8N in the QFPDD groups with different concentrations(25, 50 and 75 mg/L) was significantly higher than that in the control group(P<0.05, P<0.01) .Moreover, the number of cells with the ability to produce proplatelet particles in all QFPDD treatment groups was significantly higher than that in the control group(P<0.01), and the number of platelet-like particles in cell culture medium of all QFPDD treatment groups was significantly higher than that of the control group(P<0.01) .③The mRNA expression levels of transcription factors fNF-E2, GATA-1 and Fli-1 in the QFPDD 75 mg/L group were significantly higher than those in the control group(P<0.01), and the expression level of Fli-1 mRNA in the QFPDD 50 mg/L group was also significantly higher than that in the control group(P<0.01), but QFPDD at different concentrations showed no significant effect on the expression of aNF-E2 mRNA.Conclusion:QFPDD can promote the differentiation and polyploidization of DAMI cells, as well as the production of platelet-like particles.Its mechanism may be related to up-regulating the expressions of transcription factors fNF-E2, GATA-1 and Fli-1.关键词：Qingfei Paidu Decoction;megakaryocyte;differentiation;platelet
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发布时间：2022-09-09 - 摘要：Objective:To investigate the effects of helichrysetin on mitochondrial oxidative phosphorylation(OXPHOS) in human gastric cancer MGC-803 cells and explore its molecular mechanism.Methods:①The cells were divided into the control group and helichrysetin groups with different concentrations(10, 20, 40 μmol/L), which were treated with the corresponding intervention.After treatment for 24 and 48 hours, the related indexes of mitochondrial OXPHOS were detected by kits.②The cells were divided into the control group and helichrysetin(20 μmol/L) group, which were treated with the corresponding intervention.After treatment for 12 and 24 hours, the mRNA expressions of peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α) and melanocyte inducing transcription factor(MITF) were detected by PCR, and the protein expression of PGC-1α was detected by Western blot.③The cells were divided into the control group, helichrysetin(20 μmol/L) group, PGC-1α agonist(10 μmol/L) group and helichrysetin plus agonist group, which were treated with the corresponding intervention.After treatment for 24 hours, the cell viability was detected by CCK-8 assay.Results:①After drug intervention for 24 hours, compared with the control group, the basic respiration and proton leakage were inhibited in the helichrysetin 20 μmol/L group(P<0.05, P<0.01), and the basic respiration, maximal respiration, proton leakage, ATP production and spare respiration capacity were inhibited in the helichrysetin 40 μmol/L group(P<0.05, P<0.01) .After drug intervention for 48 hours, compared with the control group, the basal respiration, maximal respiration, proton leakage and spare respiration capacity were inhibited in the helichrysetin groups with different concentrations(P<0.05, P<0.01), and the ATP production was also significantly decreased in the helichrysetin 20 and 40 μmol/L groups(P<0.01) .②After treatment with helichrysetin at 20 μmol/L for 12 and 24 hours, the mRNA and protein expressions of PGC-1α and the mRNA expression of MITF in MGC-803 cells were significantly down-regulated(P<0.01) .③Helichrysetin at 20 μmol/L could significantly reduce the cell viability of MGC-803 cells(P<0.01), but this effect could be antagonized by PGC-1α agonist, and the cell viability of the helichrysetin plus agonist group was obviously higher than that of the helichrysetin group(P<0.01) .Conclusion:Helichrysetin may inhibit MITF/PGC-1α axis, reduce mitochondrial OXPHOS of MGC-803 cells, and then suppress the viability of tumor cells.关键词：helichrysetin;gastric cancer;mitochondrial oxdative phosphorylation;cell viability;PGC-1α
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发布时间：2022-09-09 - 摘要：Objective:To study the effects of electroacupuncture combined with vibration training on nuclear transcription factor inhibitory protein(IκB) /nuclear transcription factor κB(NF-κB) signaling pathway and motor function in hypoxic-ischemic encephalopathy(HIE) rats.Methods:Seven days old SD rats were randomly divided into the sham operation group, model group, electroacupuncture group, vibration group and combination group, 20 rats in each group.Except the sham operation group, HIE model was established by modified Rice method in the other groups.Twenty-four hours after modeling, the electroacupuncture group was treated with electroacupuncture at Baihui, Renzhong and Dazhui acupoints, 15 minutes each time; the vibration group was treated with vibration training, 15 minutes each time; and the combination group was treated with electroacupuncture and vibration training successively.The treatments were performed once a day for 2 weeks.After the last intervention, the neurobehavior of rats was evaluated by Bederson score, and the cross-sectional area of gastrocnemius muscle of left hindlimb was measured by ultrasonic diagnostic instrument.The morphological changes of brain tissue were observed under light microscope after HE staining.The mRNA expressions of IκB, NF-κB p65, Bcl-2 and Bax in gastrocnemius muscle were detected by PCR.The protein expressions of IκB, NF-κB p65, Bcl-2 and Bax in gastrocnemius muscle were detected by Western blot.Results:After treatment for 2 weeks, ①The Bederson score of rats in the electroacupuncture group, vibration group and combination group was significantly lower than that in the model group(P<0.05, P<0.01), and the score of the combination group was significantly lower than that of the vibration group and the electroacupuncture group(P<0.05) .②Compared with the model group, the cross-sectional area of left gastrocnemius muscle in the electroacupuncture group, vibration group and combination group was increased significantly(P<0.05, P<0.01) .The cross-sectional area of the combination group was significantly larger than that of the electroacupuncture group and the vibration group(P<0.05, P<0.01) .③HE staining showed that the arrangement of nerve cells in the damaged brain tissue of the model group was disordered, the intercellular space was enlarged, the structure was loose, and the vacuole-like changes, obvious cell body swelling and nuclear atrophy were observed.The pathological injury of brain tissue in the electroacupuncture group, vibration group and combination group was alleviated, and the improvement in the combination group was more significant, and the necrotic cells of brain tissue and inflammatory infiltration in ischemic area were significantly reduced.④Compared with the model group, the mRNA and protein expression levels of IκB, NF-κB p65 and Bax in gastrocnemius muscle of the electroacupuncture group, vibration group and combination group were significantly decreased, and the mRNA and protein expression levels of Bcl-2 were significantly increased(P<0.05, P<0.01) .Compared with the electroacupuncture group and vibration group, the mRNA and protein expressions of IκB, NF-κB p65 and Bax were down-regulated in the combination group, and the mRNA and protein expressions of Bcl-2 were up-regulated(P<0.05, P<0.01) .Conclusion:Electroacupuncture combined with vibration training can significantly improve neurobehavior and reduce brain tissue injury in HIE model rats, and the effect is better than electroacupuncture or vibration training alone.Its mechanism may be related to suppressing IκB/NF-κB signaling pathway, inhibiting cell apoptosis of skeletal muscle and improving motor function.关键词：electroacupuncture;vibration training;hypoxic-ischemic encephalopathy;IκB/NF-κB signaling pathway;cell apoptosis;rat
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发布时间：2022-09-09
Experiment Research
- 摘要：By analyzing the recognition of traditional Chinese medicine on the relationship between physique and spirit and the etiology and pathogenesis of restless leg syndrome(RLS), the theoretical basis of scalp cluster acupuncture in the treatment of RLS is discussed.Physique and spirit are interrelated physiologically and pathologically, and the idea of unity of physique and spirit plays an important guiding role in the diagnosis and treatment of diseases.In the course of occurrence and development of RLS, both physique and spirit show abnormal changes, therefore, both physique and spirit should be adjusted in treating RLS.Based on the idea of adjusting both physique and spirit, scalp cluster acupuncture shows significant clinical efficacy in the treatment of RLS.关键词：restless leg syndrome;adjusting both physique and spirit;scalp cluster acupuncture;characteristic therapy of traditional Chinese medicine
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发布时间：2022-09-09
Theoretical Research
- 摘要：As a commonly used Chinese medicine, Astragali Radix shows the efficacy of invigorating qi and yang, consolidating superficies and inducing diuresis, expelling toxin and discharging pus.Saponins of Astragali Radix are the main active components, with the pharmacological effects such as anti-inflammation, anti-tumor, immune regulation, metabolism regulation and organ protection.Herein, the chemical structure and pharmacological activity, biosynthetic pathway and metabolic regulation, biotransformation and synthetic biology of saponins of Astragali Radix are reviewed, and the development of its biosynthesis is prospected, so as to provide reference for the application of biotechnology to realize the large-scale production of saponins of Astragali Radix.关键词：saponins of Astragali Radix;biosynthesis;biotransformation;review
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发布时间：2022-09-09 - 摘要：Polysaccharides are important bioactive macromolecules in Chinese medicines, with various pharmacological activities such as anti-viral, anti-bacteria, anti-inflammation, anti-oxidation, immune modulation and gut microbiota regulation.In recent years, studies have found that herbal polysaccharides showed significant advantages and unique mechanisms on anti-viral and alleviation of virus-caused inflammation, which attracted extensive attention at home and abroad.This article systematically reviews the research progress of herbal polysaccharides in anti-viral and relieving related symptoms induced by viruses, including directly killing viruses, preventing virus adhesion and invasion, preventing virus replication, regulating immunity, anti-inflammation, anti-oxidation and regulating gut microbiota, so as to provide reference for scientific interpretation of the anti-viral mechanism of Chinese medicine prescriptions and the anti-viral drugs development based on herbal polysaccharides.关键词：herbal polysaccharides;anti-viral;mechanism;corona virus disease 2019 (COVID-19);review
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发布时间：2022-09-09
Review
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