最新刊期
期 4 ， 2021
- 摘要：This paper discusses the reason and symptom of toxic and side effects, safe dosage, source of medicinal materials, processing methods and quality control of Clematidis Radix et Rhizoma, through searching the ancient and modern literatures of traditional Chinese medicine and the related researches about the toxic and side effects of Clematidis Radix et Rhizoma from journal databases in recent 30 years.Clematidis Radix et Rhizoma can induce the toxic and side effects, such as allergic reaction, vomiting, diarrhea, arrhythmia and renal toxicity, even result in shock and death.When Clematidis Radix et Rhizoma is used for long period or in excess, or compatibility with Aconiti Lateralis Radix Praeparaia, or mixed with wine or vinegar, it is more likely to induce the toxic and side effects.In order to avoid the toxic and side effects, it is suggested to use it from the small dose according to Pharmacopoeia of the People’s Republic of China.The components that induce the toxic and side effects might be anemonin, protoanemonin, volatile oil, saponins and so on, or mixed aristolochic acid due to contamination by other herbal medicines.In addition, investigation on the processing methods and local medicinal materials of Clematidis Radix et Rhizoma is of great significance for further study of its toxic and side effects, safe use and expansion of drug source.关键词：Clematidis Radix et Rhizoma;toxic and side effects;safe dosage;toxic component;quality control standard
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发布时间：2022-09-09
Scholar Forum
- 摘要：Objective:To observe the clinical efficacy of Qiangxin Recipe combined with conventional western medicine in the treatment of chronic heart failure(CHF) with syndrome of heart and kidney yang deficiency.Methods:A total of 82 CHF patients with syndrome of heart and kidney yang deficiency who met the inclusion criteria were randomly divided into the treatment group(42 cases) and control group(40 cases) .The patients in both groups were treated with conventional western medicine, and the patients in the treatment group were orally treated with modified Qiangxin Recipe based on conventional western medicine.The treatment course of the two groups was 3 months.The efficacy of cardiac function classification improvement and Chinese medical syndrome were compared between the two groups.Before and after treatment, the left ventricular end diastolic diameter(LVEDD), left ventricular end systolic diameter(LVESD), left ventricular ejection fraction(LVEF), fractional shortening(FS), end diastolic volume(EDV), stroke volume(SV) and cardiac output(CO) were measured and calculated, the levels of plasma brain natriuretic peptide(BNP), cardiac troponin 1(cTn1) and serum homocysteine(Hcy) were detected, and the 6-minute walking distance(6MWD) of the two groups was compared.Results:①After treatment, the total effective rate on cardiac function classification improvement was 73.81% in the treatment group and 52.50% in the control group.The efficacy of the treatment group was better than that of the control group(P<0.05) .②After treatment, the total effective rate on Chinese medical syndrome was 78.57% in the treatment group and 50.00% in the control group.The efficacy of the treatment group was better than that of the control group(P<0.05) .③After treatment, the LVEDD and LVESD in the treatment group were reduced compared with those before treatment(P<0.05), and the LVEF and SV were increased compared with those before treatment(P<0.05, P<0.01) ; the LVEF in the control group was also increased compared with that before treatment(P<0.05) .After treatment, the LVEDD and LVESD in the treatment group were lower than those in the control group(P<0.05, P<0.01), and the LVEF was higher than that in the control group(P<0.01) .④After treatment, the levels of plasma BNP, cTn1 and serum Hcy were significantly decreased in both groups compared with those before treatment(P<0.01), and the levels of BNP and Hcy in the treatment group were significantly lower than those in the control group(P<0.01) .⑤After treatment, the 6MWD of the two groups was significantly prolonged compared with that before treatment(P<0.01), and the 6MWD of the treatment group was significantly longer than that of the control group(P<0.05) .Conclusion:Compared with the conventional western medicine, Qiangxin Recipe combined with conventional western medicine can better improve the clinical symptoms and cardiac function of CHF patients with syndrome of heart and kidney yang deficiency, delay the progress of ventricular remodeling, and regulate the levels of relevant neuroendocrine indexes.关键词：chronic heart failure;syndrome of heart and kidney yang deficiency;Qiangxin Recipe;integrated traditional Chinese and western medicine
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发布时间：2022-09-09 - 摘要：Objective:To explore the effects of modified Shuzao Yinzi Recipe on cardiac function, myocardial remodeling and serum inflammatory factors in patients with chronic heart failure(CHF) .Methods:A total of 86 patients with CHF were included and randomly divided into the observation group and control group according to random number table, 43 cases in each group.The patients in the observation group were treated with modified Shuzao Yinzi Recipe combined with conventional western medicine, and the patients in the control group were treated with conventional western medicine.The treatment course was 4 weeks.The clinical efficacy of the two groups was evaluated and compared.Before and after treatment, the left ventricular ejection fraction(LVEF), ratio of maximum early diastolic flow velocity of tricuspid valve to maximum atrial systolic flow velocity of tricuspid valve(E/A ratio) and cardiac output(CO) were detected and 6-minute walking distance(6MWD) was measured to evaluate the cardiac function.Before and after treatment, the levels of angiotensinⅡ(AngⅡ), aldosterone(ALD), brain natriuretic peptide(BNP), hypersensitive C-reactive protein(hs-CRP), tumor necrosis factor α(TNF-α) and homocysteine(Hcy) were detected to evaluate the myocardial remodeling and inflammation status.Results:①After treatment, the total effective rate on cardiac function improvement was 93.02% in the observation group and 81.40% in the control group.The efficacy of the observation group was better than that of the control group(P<0.05) .②After treatment, the LVEF, CO, E/A ratio and 6MWD in the two groups were significantly increased compared with those before treatment(P<0.05, P<0.01), and the LVEF, CO, E/A ratio and 6MWD in the observation group were significantly higher than those in the control group(P<0.05, P<0.01) .③After treatment, the levels of AngⅡ, ALD and BNP in the two groups were significantly decreased compared with those before treatment(P<0.01), and the levels of AngⅡ, ALD and BNP in the observation group were significantly lower than those in the control group(P<0.01) .④After treatment, the levels of hs-CRP, TNF-α and Hcy in the two groups were significantly decreased compared with those before treatment(P<0.05, P<0.01), and the levels of hs-CRP, TNF-α and Hcy in the observation group were significantly lower than those in the control group(P<0.05, P<0.01) .⑤The evaluation results of random walking model showed that the improvements of cardiac function, myocardial remodeling and serum inflammatory factors in the observation group were more significant than those in the control group.Conclusion:Modified Shuzao Yinzi Recipe combined with conventional western medicine therapy can better improve the cardiac function and reduce the myocardial remodeling indexes and serum inflammatory factors levels in patients with CHF, which is worth popularizing in clinical practice.关键词：chronic heart failure;Shuzao Yinzi Recipe;cardiac function;myocardial remodeling;serum inflammatory factor;integrated traditional Chinese and western medicine
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发布时间：2022-09-09 - 摘要：Objective:To observe the efficacy and safety of Wenshen Jianpi Recipe hot compress in the treatment of amyotrophic lateral sclerosis(ALS) .Methods:Sixty-nine patients with ALS were selected and randomly divided into the treatment group(n=35) and control group(n=34) .The patients in both groups were treated with conventional medicine, and the patients in the treatment group were treated with Wenshen Jianpi Recipe hot compress based on above.The treatment course was 4 months.Before and after treatment, the scores of ALS symptom scale in integrative treatment(ALS-SSIT) and ALS functional rating scale(ALS-FRS) were compared between the two groups, and the safety of treatment was evaluated.Results:In the course of trial, 3 patients in the treatment group and 4 patients in the control group were excluded.Finally 32 cases in the treatment group and 30 cases in the control group were included for the statistical analysis.After treatment, the ALS-SSIT scores in the two groups were increased(P<0.05), while the score of the treatment group was lower than that of the control group(P<0.05) .After treatment, the ALS-FRS scores in the two groups were decreased(P<0.05), while the score of the treatment group was higher than that of the control group(P<0.05) .In the course of treatment, skin itching caused by Wenshen Jianpi Recipe hot compress was observed in 7 cases.Among, 2 cases discontinued the therapy and 5 cases continued the therapy.Other adverse reactions were not found.Conclusion:As an adjuvant treatment method, Wenshen Jianpi Recipe hot compress may delay the development of ALS symptoms to a certain degree, with good safety.关键词：amyotrophic lateral sclerosis;Wenshen Jianpi Recipe hot compress;integrated traditional Chinese and western medicine;randomized controlled trial
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发布时间：2022-09-09 - 摘要：Objective:To compare the clinical efficacy between lift-up submucosal hemorrhoidectomy and traditional Milligan-Morgan hemorrhoidectomy in the treatment of annular mixed hemorrhoids.Methods:A total of 90 patients with annular mixed hemorrhoids were included and randomly divided into the treatment group and control group, 45 cases in each group.The patients in the treatment group were treated with therapy of lift-up submucosal hemorrhoidectomy, and the patients in the control group were treated with therapy of Milligan-Morgan hemorrhoidectomy.Three months after operation, the clinical efficacy of the two groups were evaluated and compared.On the 1st, 3rd, 7th and 14th days after operation, the scores of anal pain, bleeding, wound edema, anal distension and defecation were evaluated.Three months after operation, the Wexner scale was applied to evaluate the anal function, and the indexes of anorectal pressure including rectal resting pressure, anal resting pressure and maximal anal contraction pressure were detected.The operation time, hospitalization time, wound healing time and hospitalization cost of the two groups were compared.Results:①Three months after operation, the clinical total effective rates of the two groups were 100%, with no statistically significant difference between the two groups(P>0.05) .②On the 1st, 3rd, 7th and 14th days after operation, the score of anal pain in the treatment group was lower than that in the control group(P<0.05, P<0.01) .On the 1st, 3rd and 7th days after operation, the scores of bleeding and anal distension in the treatment group were lower than those in the control group(P<0.05, P<0.01) .On the 3rd day after operation, the score of wound edema in the treatment group was also lower than that in the control group(P<0.05) .③Three months after operation, the anal resting pressure in the treatment group was significantly lower than that in the control group(P<0.01) .④The operation time, hospitalization time and wound healing time in the treatment group were significantly shorter than those in the control group(P<0.01), and the hospitalization cost was significantly less than that in the control group(P<0.01) .Conclusion:Compared with Milligan-Morgan hemorrhoidectomy, lift-up submucosal hemorrhoidectomy shows good clinical efficacy in the treatment of annular mixed hemorrhoids, and can effectively alleviate the symptoms such as postoperative pain, bleeding, wound edema and anal distension, and restore the anal function, with good economy.关键词：annular mixed hemorrhoids;lift-up submucosal hemorrhoidectomy;Milligan-Morgan hemorrhoidectomy
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发布时间：2022-09-09
Clinical Research
- 摘要：Objective:To study the effects of short-term administration of cisplatin on syndrome and steroidogenesis in mice.Methods:Forty male ICR mice were randomly divided into the control group and cisplatin groups with different doses(3, 5, 10 mg·kg-1·d-1), 10 mice in each group.The drug intervention groups were intraperitoneally injected with cisplatin at the corresponding dose for 5 days.After last administration and withdrawal for 5 days, the signs(body mass, food intake, axillary temperature and claw color) of mice were observed, and the behavioral tests(grip, open field test and rotarod test) were performed.After observation period, the serum was taken, the kidney, heart, thymus and spleen were separated and the organ index was calculated, and the adrenal gland and testis were collected.The serum levels of corticosterone and testosterone were detected by ELISA.The morphological changes of adrenal gland and testis were observed under light microscope after HE staining.The expressions of genes and proteins related to steroid hormone synthetase and testosterone synthetase were detected by PCR and Western blot respectively.Results:①After administration for 5 days, the body mass, food intake, axillary temperature, horizontal movement and vertical movement in the cisplatin 10 mg·kg-1·d-1 group were significantly decreased compared with those in the control group(P<0.05, P<0.01) ; the axillary temperature of the cisplatin 3 and 5 mg·kg-1·d-1 groups was significantly lower than that of the control group, while the r value of claw color was higher than that of the control group(P<0.05, P<0.01) .After withdrawal for 5 days, the body mass, food intake, stay time, grip, axillary temperature, r value of claw color, horizontal movement and vertical movement in the cisplatin 10 mg·kg-1·d-1 group were significantly decreased compared with those in the control group(P<0.05, P<0.01) ; and the vertical movement of the cisplatin 3 and 5 mg·kg-1·d-1 groups was also decreased compared with that of the control group(P<0.01) .②Compared with the control group, cisplatin at dose of 10 mg·kg-1·d-1 significantly inhibited the spleen, thymus and heart of mice, these organ indexes were decreased significantly(P<0.05, P<0.01), while the kidney index was increased significantly(P<0.01) ; cisplatin at dose of 5 mg·kg-1·d-1 also inhibited the heart(P<0.05) .③The morphology showed that, the cells in the glomerular zone of adrenal cortex swelled obviously in the cisplatin 10 mg·kg-1·d-1 group, the arrangement of spermatogenic cells at all levels was slightly disordered, and the leydig cells of testis contracted obviously in the cisplatin 5 and 10 mg·kg-1·d-1 groups.④Compared with the control group, the serum content of corticosterone was significantly increased in the cisplatin 10 mg·kg-1·d-1 group(P<0.01), while there was no significant change in serum testosterone.⑤Compared with the control group, the mRNA expressions of Star and Cyp21a1 in the cisplatin 3 mg·kg-1·d-1 group was inhibited(P<0.05), while the mRNA expressions of Star, Cyp11b1 and Cyp21a1 and the protein expressions of StAR, CYP21A2 and CYP11B1 were significantly up-regulated in the cisplatin 10 mg·kg-1·d-1 group(P<0.05, P<0.01) .⑥Compared with the control group, cisplatin at dose of 10 mg·kg-1·d-1 significantly inhibited the mRNA and protein expressions of StAR, CYP11A1, CYP17A1 and HSD3B2 in testis(P<0.05, P<0.01), cisplatin at dose of 5 mg·kg-1·d-1 significantly inhibited the mRNA expressions of Star and Hsd3b2(P<0.05, P<0.01), and cisplatin at dose of 3 mg·kg-1·d-1 significantly inhibited the mRNA expressions of Cyp17a1 and Hsd3b2(P<0.01) .Conclusion:Short-term administration of cisplatin can induce deficiency syndrome in mice, which mainly shows the deficiency of essence and qi, and its material basis is related to the decrease of steroid hormone synthesis and reserve.关键词：cisplatin;deficiency syndrome;drug-induced syndrome;adrenal cortex;testis;mouse
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发布时间：2022-09-09
Discipline Frontiers
- 摘要：Objective:To establish the myotube atrophy model of C2C12 cells based on co-culture system of C26 colon cancer cells and RAW264.7 macrophages, and study the protective effect of glycyrrhiza polysaccharides on myotubes and its mechanism.Methods:The co-culture system of C26 cells and RAW264.7 cells was constructed, and the conditioned medium of single cells and co-culture cells was prepared, respectively.The differentiation of myotubes was induced from C2C12 myoblasts.①The differentiated C2C12 cells were divided into the control group, C26 model group, RAW264.7 model group and co-culture model group.Each group was treated with the corresponding conditioned medium.After incubation for 48 hours, the morphology of myotubes was observed after HE staining, and the diameter of myotubes was determined.②The differentiated C2C12 cells were divided into the control group and glycyrrhiza polysaccharides groups with different concentrations(0.312 5, 0.625, 1.25, 2.5 and 5 mg/ml) .Each group was treated with glycyrrhiza polysaccharides at the corresponding concentration.After incubation for 48 hours, the cell viability was measured.③The differentiated C2C12 cells were divided into the control group, C26 model group and C26+glycyrrhiza polysaccharides groups with different concentrations.Except the control group, the other groups were treated with the conditioned medium of C26 colon cancer cells.Meanwhile, the glycyrrhiza polysaccharides intervention groups were treated with glycyrrhiza polysaccharides at concentration of 0.5, 1, 2 mg/ml, respectively.After incubation for 48 hours, the morphology of myotubes was observed after HE staining, and the diameter of myotubes was determined.④The differentiated C2C12 cells were divided into the control group, co-culture model group and co-culture+glycyrrhiza polysaccharides groups with different concentrations.Except the control group, the other groups were treated with the conditioned medium of co-culture cells.Meanwhile, the glycyrrhiza polysaccharides intervention groups were treated with glycyrrhiza polysaccharides at concentration of 0.5, 1, 2 mg/ml, respectively.After incubation for 48 hours, the morphology of myotubes was observed after HE staining, and the diameter of myotubes was determined.⑤After treatment with different conditioned medium or co-treatment of co-culture conditioned medium and glycyrrhiza polysaccharides(2 mg/ml), the protein expression and phosphorylation of signal transducer and activator of transcription 3(STAT3) were detected by Western blot.Results:①All three kinds of conditioned medium could induce the decrease of myotube diameter(P<0.01), and the relative myotube diameter of the co-culture model group was significantly lower than that of the C26 model group(P<0.05) .②Glycyrrhiza polysaccharides≤2.5 mg/ml showed no significant effect on the cell viability.③Compared with C26 model group, the relative myotube diameter of C26+glycyrrhiza polysaccharides(0.5, 1 and 2 mg/ml) groups was significantly increased(P<0.01) .④Compared with co-culture model group, the relative myotube diameter of co-culture+glycyrrhiza polysaccharides(0.5, 1 and 2 mg/ml) groups was significantly increased(P<0.01) .⑤All three conditioned medium could induce the increase of STAT3 phosphorylation level in myotubes, and the protein expression ratio of p-STAT3/STAT3 in the co-culture model group was obviously higher than that in the C26 model group(P<0.01) .Compared with co-culture model group, the protein expression ratio of p-STAT3/STAT3 was significantly down-regulated in the co-culture+glycyrrhiza polysaccharides(2 mg/ml) group(P<0.01) .Conclusion:Glycyrrhiza polysaccharides may alleviate the myotube atrophy induced by co-culture system of C26 colon cancer cells and RAW264.7 macrophages via inhibiting STAT3 signaling pathway.关键词：glycyrrhiza polysaccharides;cancer cachexia;tumor microenvironment;co-culture;muscle atrophy
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发布时间：2022-09-09 - 摘要：Objective:To investigate the effects of cardamonin on the glucose uptake capacity in human triple negative breast cancer MDA-MB-231 cells and explore its molecular mechanism.Methods:MDA-MB-231 cells were divided into the cardamonin groups with different concentrations(0, 10, 20, 40, 80 μmol/L) .Each group was treated with cardamonin at the corresponding concentration for 24 hours.The cell viability was detected by CCK-8 assay.The glucose uptake capacity was evaluated by 2-NBDG assay.The mRNA expressions of glucose transporters(GLUT) were detected by PCR.The protein expressions of GULT were detected by Western blot.MDA-MB-231 cells were subcutaneously inoculated to construct tumor bearing nude mice model.After intraperitoneal injection of cardamonin(0.3, 1.0, 3.0 mg/kg) for 4 weeks, the breast cancer tissue of tumor bearing mice was isolated, and the expressions of GLUT proteins in the breast cancer tissue were detected by Western blot.Results:Cardamonin(20, 40, 80 μmol/L) significantly inhibited the viability of MDA-MB-231 cells in a dose-dependent manner(P<0.01), and cardamonin at concentration of 20 μmol/L significantly reduced the glucose uptake capacity of breast cancer cells(P<0.05) .Further studies showed that, cardamonin at concentration of 20 μmol/L significantly down-regulated the mRNA expressions of GLUT3 and GLUT4 in breast cancer cells(P<0.01) .Cardamonin at concentration of 40 μmol/L significantly down-regulated the protein expression of GLUT3 in breast cancer cells(P<0.01), cardamonin(10, 20, 40 μmol/L) significantly down-regulated the protein expression of GLUT4 in breast cancer cells(P<0.01), but showed no obvious effect on GLUT1 protein expression(P>0.05) .Cardamonin(0.3, 1.0, 3.0 mg/kg) significantly reduced the protein expression of GLUT1 in breast cancer tissue of tumor bearing mice(P<0.01), while cardamonin(1.0, 3.0 mg/kg) significantly down-regulated the protein expression of GLUT3(P<0.01) .Conclusion:Cardamonin may decrease the cell viability and glucose uptake capacity of human triple negative breast cancer MDA-MB-231 cells by down-regulating the mRNA and protein expressions of GLUT.关键词：cardamonin;breast cancer;cell viability;glucose uptake;glucose transporter
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发布时间：2022-09-09 - 摘要：Objective:To investigate the neuroprotective effects of isorhynchophylline(IsoRhy), extract of Uncariae Ramulus Cum Uncis, on 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine(MPTP) -induced Parkinson’s disease(PD) model mice.Methods:C57BL/6 mice were randomly divided into the control group, MPTP group, Rasagiline(0.1 mg/kg) group and IsoRhy(40 mg/kg) group, 8 mice in each group.Except the control group, the other groups were intraperitoneally injected with 30 mg/kg MPTP for 5 days, so as to induce PD model.From the 8th day of the experiment, the drug treatment groups were treated with the corresponding drugs by intragastric administration for two weeks.After the last administration, behavioral tests were performed and brain tissue was separated.The contents of dopamine(DA) and its metabolites in striatum were determined by high performance liquid chromatography with electrochemical detection(HPLC-ECD) .The number of tyrosine hydroxylase(TH) positive neurons in substantia nigra was detected by immunohistochemistry, and the TH protein expression in substantia nigra was detected by Western blot.Results:①Pole test: The climbing time of mice in the IsoRhy group was significantly shorter than that in the MPTP group(P<0.01) .Footprint test: IsoRhy could effectively increase the stride length of the model mice(P<0.01), but showed no significant effect on the passing time.Open field test: The movement distance of mice in the IsoRhy group was significantly longer than that in the MPTP group(P<0.01) .Rotarod test: The stay time of mice in the IsoRhy group was longer than that in the MPTP group, but with no statistically significant difference(P>0.05) .②The contents of DA and its metabolites 3, 4-dihydroxyphenylacetic acid(DOPAC) and homovanillic acid(HVA) in striatum of the IsoRhy group were higher than those of the MPTP group, but with no statistically significant differences(P>0.05) .③After treatment with IsoRhy for two weeks, the TH positive neurons in substantia nigra of the model mice were significantly increased compared with those in the MPTP group(P<0.05), and the TH protein expression in substantia nigra was significantly higher than that in the MPTP group(P<0.05) .Conclusion:IsoRhy can improve the motor behavior of MPTP-induced PD model mice to a certain extent, and alleviate the damage of dopaminergic neurons by up-regulating the TH protein expression in substantia nigra.关键词：isorhynchophylline;Parkinson’s disease;MPTP;neuroprotection;mouse
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发布时间：2022-09-09 - 摘要：Objective:To investigate the protective effects of 3, 4-dihydroxybenzaldehyde(3, 4-DD), an active component of Gastrodiae Rhizoma, on oxygen-glucose deprivation/reperfusion(OGD/R) injury in brain microvascular endothelial cells(BMEC) and neurons by acting on astrocytes(Ast), from the perspective of interconnection between neurovascular unit(NVU) cells.Methods:BMEC and Ast were isolated and cultured from neonatal rats.The co-culture systems of Ast and BMEC, Ast and PC12 cells were constructed by Transwell chamber, respectively.The co-culture cells were divided into the control group, model group, butyphthalide(NBP) intervention(1×10-4mol/L) group and 3, 4-DD intervention(1×10-7mol/L) group.The drug treatment groups were treated with the corresponding drug in advance.After drug intervention for 24 hours, OGD/R injury was induced in the model group and drug treatment groups.The transendothelial electrical resistance(TEER) of the cells inside Transwell chamber was measured by cell electrical resistance meter.The activity of lactate dehydrogenase(LDH) in BMEC and PC12 cells was detected by kit.The content of brain-derived neurotrophic factor(BDNF) in the medium was detected by ELISA.The mRNA expressions of Occludin in BMEC and phospholipase C-γ(Plc-γ) in PC12 cells were detected by PCR.Results:①Ast-BMEC co-culture system: Compared with the control group, the TEER value, LDH activity and BDNF content of the model group were significantly decreased(P<0.01), and the Occludin mRNA expression was significantly increased(P<0.01) .Compared with the model group, the TEER value, LDH activity, BDNF content and Occludin mRNA expression were significantly increased in the 3, 4-DD intervention group(P<0.01), and the LDH activity, BDNF content and Occludin mRNA expression were also significantly increased in the NBP intervention group(P<0.01) .The TEER value of 3, 4-DD intervention group was significantly higher than that of NBP intervention group(P<0.01) .②Ast-PC12 co-culture system: Compared with the control group, the TEER value, LDH activity and BDNF content of the model group were significantly decreased(P<0.01), and the Plc-γ mRNA expression was significantly increased(P<0.01) .Compared with the model group, the LDH activity, BDNF content and Plc-γ mRNA expression were significantly increased in the 3, 4-DD intervention group(P<0.05, P<0.01), and the LDH activity and Plc-γ mRNA expression were also significantly increased in the NBP intervention group(P<0.01) .Conclusion:3, 4-DD can promote the release of BDNF by Ast, act on BMEC and neurons, maintain intercellular structure, enhance synaptic plasticity of neurons, and then alleviate OGD/R injury.关键词：3, 4-dihydroxybenzaldehyde;oxygen-glucose deprivation/reperfusion;brain-derived neurotrophic factor;astrocyte;brain microvascular endothelial cell
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发布时间：2022-09-09 - 摘要：Objective:To explore the effects of Kushenol F on oxidative stress and autophagy in melanocytes.Methods:PIG1 cell line was used as the research object.The cells were divided into the control group, model group, positive control(berberine, 5 μmol/L) group and Kushenol F groups with low-(26 μmol/L), middle-(52 μmol/L) and high-(78 μmol/L) dose.Except the control group, the other groups were treated with 1.0 mmol/L hydrogen peroxide(H2O2) for 24 hours to induce oxidative stress injury.After H2O2 stimulation, each drug group was treated with the corresponding drug for 24 hours.The cell viability was detected by CCK8 assay.The levels of reactive oxygen species(ROS), malondialdehyde(MDA) and glutathione(GSH) were detected by ELISA.The autophagosomes were observed by transmission electron microscope.The expression of E-cadherin was detected by immunofluorescence.The expressions of oxidative stress and autophagy related proteins were detected by Western blot, including Beclin-1, P62, microtubule-associated protein 1 light chain 3(LC3) and nuclear factor erythroid 2-related factor 2(Nrf2) .Results:①Compared with the model group, the cell viability of Kushenol F groups with different doses was significantly increased(P<0.01), and the cell viability of high-dose group was significantly higher than that of low-dose group and positive control group(P<0.05) .②Compared with the model group, the levels of ROS and MDA were significantly decreased(P<0.05, P<0.01) and the level of GSH was significantly increased(P<0.05, P<0.01) in the Kushenol F groups with different doses.The levels of ROS and MDA in the high-dose group were lower than those in the low-dose group and positive control group((P<0.05, P<0.01), and the level of GSH in the high-dose group was higher than that in the low-dose group(P<0.05) .③Compared with the model group, the number of autophagosomes in the Kushenol F groups with different doses was significantly increased(P<0.05, P<0.01) ; and the number of autophagosomes in the middle-and high-dose groups was significantly more than that in the low-dose group(P<0.05, P<0.01) .④The expression of E-cadherin in the Kushenol F groups with middle-and high-dose was significantly higher than that in the model group(P<0.05, P<0.01), and was significantly higher than that in the low-dose group(P<0.05, P<0.01) .⑤Compared with the model group, after treatment with Kushenol F at different doses, the protein expression levels of Beclin-1, LC3Ⅱ/LC3Ⅰ, Nrf2 and P62 were significantly increased(P<0.05, P<0.01) .Conclusion:Kushenol F may up-regulate Nrf2-P62 signaling pathway, promote melanocyte autophagy, and thus resist oxidative stress injury.关键词：Kushenol F;vitiligo;melanocyte;oxidative stress;autophagy
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发布时间：2022-09-09 - 摘要：Objective:To observe the effects of Huayu Bushen Formula on fracture healing and oxidative stress in ovariectomized mice.Methods:Three months old female C57BL/6 mice were randomly divided into the sham operation group, model group and Huayu Bushen Formula group, 10 mice in each group.Bilateral ovariectomy(OVX) was performed in the model group and Huayu Bushen Formula group, while only part of the adipose tissue around the ovary was removed in the sham operation group.Three months after operation, the model of left tibial fracture combined with intramedullary fixation was established in all groups.After modeling, mice in the Huayu Bushen Formula group were treated with decoction of Huayu Bushen Formula at dose of 7.33 g/kg by intragastric administration for 14 days, while mice in the sham operation group and model group were treated with 0.9% NaCl solution at equal volume.After last administration, blood was taken and tibia tissue was separated.The fracture healing was observed by X-ray and Micro-CT scanning.After alcian blue/orange G staining, the histomorphological changes of callus were observed under microscope.The serum levels of superoxide dismutase(SOD) and malondialdehyde(MDA) were measured by kits.The expression of CollagenⅠ in callus was detected by immunohistochemistry.The expressions of Runt-related transcription factor 2(Runx2) and SOD in callus were detected by immunofluorescence.The content of MDA in callus was detected by kit.The cell apoptosis in callus was detected by TUNEL assay.Results:①X-ray and Micro-CT examination of tibia showed that, the calluses were few and scattered and the fracture line was clear in the model group, while the calluses were dense and the fracture line was slightly blurred in the Huayu Bushen Formula group.②Alcian blue/orange G staining showed that, there were more cartilage matrix and less bone trabecula in callus of the model group, but less cartilage matrix and dense bone trabecular were observed in callus of the Huayu Bushen Formula group.③The expressions of Runx2 and CollagenⅠ in callus of the model group were significantly lower than those of the sham operation group(P<0.01), and the expressions of Runx2 and CollagenⅠ in callus of the Huayu Bushen Formula group were significantly higher than those of the model group(P<0.05) .④The cell apoptosis of callus tissue in the Huayu Bushen Formula group was significantly decreased compared with that in the model group(P<0.01) .⑤Compared with the sham operation group, the SOD level in serum and callus tissue of the model group was significantly decreased(P<0.01), and the MDA content was significantly increased(P<0.01) .Compared with the model group, the SOD level in serum and callus tissue of the Huayu Bushen Formula group was significantly increased(P<0.05), and the MDA content was significantly decreased(P<0.01) .Conclusion:Huayu Bushen Formula can promote the fracture healing in ovariectomized mice, and its mechanisms may be related to increasing the levels of antioxidant enzymes in serum and local callus, alleviating oxidative stress and inhibiting cell apoptosis.关键词：Huayu Bushen Formula;osteoportic fracture;oxidative stress;mouse
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发布时间：2022-09-09 - 摘要：Objective:To discuss the effects of simulated acupuncture at different time points on systolic blood pressure and expressions of myocardial circadian clock genes in spontaneous hypertensive rats(SHR), including brain and muscle arnt-like protein 1(Bmal1) 、circadian locomotor output cycles kaput(Clock) and period 2(Per2) .Methods:Twenty-four male SHR were randomly divided into the SHR model group, acupuncture at 14: 00 group and acupuncture at 21: 00 group, 8 rats in each group.Another 7 male WKY rats were used as control.Bilateral Zusanli and Quchi acupoints were selected, with the aid of ATP-1 simulated acupuncture manipulation instrument, the rats in the acupuncture at 14: 00 group and acupuncture at 21: 00 group were treated with simulated acupuncture at the corresponding time points, once every other day for 10 times.The systolic blood pressure of tail artery was measured before and after treatment.After the last intervention, the myocardial tissue was isolated.The mRNA expressions of Bmal1, Clock and Per2 in the myocardial tissue were detected by PCR, and the protein expressions of Bmal1, Clock and Per2 were detected by Western blot.Results:①After treatment, compared with the SHR model group, the systolic blood pressure of rats in the acupuncture at 14: 00 group and acupuncture at 21: 00 group was decreased significantly(P<0.01), and the systolic blood pressure of the acupuncture at 21: 00 group was significantly lower than that of the acupuncture at 14: 00 group(P<0.01) .After treatment, compared with that before treatment, the systolic blood pressure of rats in the acupuncture at 21: 00 group was decreased significantly(P<0.01) .②Compared with the SHR model group, the mRNA expression levels of Bmal1, Clock and Per2 in the myocardial tissue were significantly down-regulated in the acupuncture at 14: 00 group and acupuncture at 21: 00 group(P<0.05) .The mRNA expression levels of Clock and Per2 in the acupuncture at 21: 00 group were significantly lower than those in the acupuncture at 14: 00 group(P<0.05) .③Compared with the SHR model group, the protein expression levels of Bmal1, Clock and Per2 in the myocardial tissue were significantly down-regulated in the acupuncture at 14: 00 group and acupuncture at 21: 00 group(P<0.05) .The protein expression levels of Bmal1, Clock and Per2 in the acupuncture at 21: 00 group were significantly lower than those in the acupuncture at 14: 00 group(P<0.01) .Conclusion:Simulated acupuncture can reduce the systolic blood pressure of SHR and down-regulate the mRNA and protein expression levels of Bmal1, Clock and Per2 in the myocardial tissue, and the antihypertensive effect of simulated acupuncture at 21: 00 is better than that of simulated acupuncture at 14: 00.关键词：simulated acupuncture;hypertension;circadian clock gene;rat
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发布时间：2022-09-09
Experiment Research
- 摘要：Colorectal cancer is a common malignant tumor in clinic.At present, the treatment methods mainly include surgery, radiotherapy, chemotherapy and targeted therapy.With the clinical application of immune checkpoint inhibitors, the therapy for colorectal cancer has entered the era of immunotherapy.As a part of comprehensive treatment on cancer, traditional Chinese medicine is widely used in the treatment of colorectal cancer.The regulation of traditional Chinese medicine on the immune microenvironment of colorectal cancer is one of the main ways to play its therapeutic role, which mainly manifests in the regulation of adaptive immunity mediated by T cells and immune response mediated by innate immune cells.This paper reviews the influence of immune microenvironment on the occurrence and development of colorectal cancer, as well as the regulation of traditional Chinese medicine on the immune microenvironment of colorectal cancer, so as to provide ideas for the research of tumor immunotherapy with traditional Chinese medicine.关键词：colorectal cancer;immune microenvironment;traditional Chinese medicine;review
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发布时间：2022-09-09
Review
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