HU Junwei,JIN Zhu,LI Xu,et al.Study on acupoint positioning method of "Ciliao (BL32)" in mice[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2024,38(04):55-61.
HU Junwei,JIN Zhu,LI Xu,et al.Study on acupoint positioning method of "Ciliao (BL32)" in mice[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2024,38(04):55-61. DOI: 10.16306/j.1008-861x.2024.04.008.
Study on acupoint positioning method of "Ciliao (BL32)" in mice
Objective: To investigate the positioning method of "Ciliao (BL32)" acupoint in mice, and provide a precise and feasible approach for Ciliao (BL32) acupoint location in mice.
Methods
2
①Healthy adult C57BL mice were taken, and the coordinate point of Ciliao (BL32) was determined by dissection. ②Adult C57BL mice were selected and randomly divided into the experimental group and control group, and the coordinate positioning method and the acupoint positioning method with "L6 spinous process" as the bony sign were respectively applied for acupiont selection. The accuracy of the two acupoint selection methods were compared. ③Adult C57BL mice were selected and the interstitial cystitis (IC) model of mouse was copied. The coordinate positioning method was applied for treatment and its functional effect was verified.
Results
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The coordinates of Ciliao (BL32) acupoint of healthy adult mice were (±1 mm, 21 mm, -5 mm), and the accuracy of the coordinate positioning method was higher than that of the bone sign positioning method. Meanwhile the electroacupuncture treatment applying the coordinate positioning method could improve the related symptoms of IC mice.
Conclusion
2
The accuracy of the coordinate positioning method is higher than that of the bone sign positioning method, and has the function of Ciliao (BL32) acupoint.
WU J,CHENG Y,QIN Z,et al. Effects of electroacupuncture on bladder and bowel function in patients with transverse myelitis: a prospective observational study[J]. Acupunct Med, 2018, 36(4): 261-266.
ZHANG W,FANG Y,SHI M,et al. Optimal acupoint and session of acupuncture for patients with chronic prostatitis/chronic pelvic pain syndrome: a meta-analysis[J].Transl Androl Urol, 2021, 10(1): 143-153.
WANG S J,YANG H Y,WANG F,et al. Acupoint Specificity on Colorectal Hypersensitivity Alleviated by Acupuncture and the Correlation with the Brain-Gut Axis[J]. Neurochem Res, 2015, 40(6): 1274-1282.
Chinese Society of Acupuncture-Moxibustion. Name and location of commonly used acupoints in laboratory animals - Part 3: Mice (T/CAAM 0003-2020)[S]. Acupuncture Research, 21, 46(5): 445-446.
Chinese Society of Acupuncture-Moxibustion. Name and location of commonly used acupoints in laboratory animals Part 2: rats (T/CAAM 0002-2020)[S]. Acupuncture Research, 21, 46(4): 351-352.
LIUX M, XU M, DENG S F, et al. Study onacupoint positioning method ofCiliao(BL32) in rats[J]. Journal of Hunan University of Chinese Medicine, 2019, 39 (11): 1373-1376.
Paxinos G,Franklin K B J.Paxinos and Franklin's the Mouse Brain in Stereotaxic Coordinates[M]. Amsterdam: Elsevier/A P,2013.
BAO X Q, HUANG Y C, WEI S S, et al. Effects of phycocyanin on the cyclophosphamide-induced IC/BPS in murine models[J]. Journal of Modern Urology, 2019, 24 (6): 480-484.
LIU J D, FENG J W, HU J W, et al. Effects of acupuncture on PIEZO1-mediated mechanical stimulation of bladder urothelium in rats with overactive bladder[J]. Lishizhen Medicine and Materia Medica Research, 2022, 33 (10): 2539-2542.
GAUDET A D,FONKEN L K,AYALA M T,et al. Aging and miR-155 in mice influence survival and neuropathic pain after spinal cord injury[J]. Brain Behav Immun, 2021, 97: 365-370.
LU Y,LI J,DONG L,et al. Activation of uroepithelial 5-HT(4)R inhibits mechanosensory activity of murine bladder afferent nerves[J]. Front Physiol, 2022, 13: 990178.
WANG L L,REN Z X,ZHU J Y,et al. Efficacy of electroacupuncture combined with penetrating moxibustion for postpartum stress urinary incontinence[J]. Zhongguo Zhen Jiu,2019, 39(6): 599-603.
XU G X, LUO L J, DENG Z H, et al. Research on acupointregularity of acupunctureand moxibustion treatment for BPH in recent 10 Years:A date mining based on clusteringand association rules[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology, 20, 22(4): 1330-1340.
HE T, YANG S, MO Q. Analysis of the characteristics and rules of acupoints selection of acupuncture and moxibustion treatment of overactivitybladder[J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology,2018, 20(6): 960-966.
ZHANG P X, KOU W P. Effect of acupuncture at Ciliao(BL32)on pain and edema after anorectal surgery and changes in levels ofrelated pain mediators and inflammatory factors [J]. Chinese Archives of Traditional Chinese Medicine, 202, 40(10): 87-90.
黎文献,薛长利,黎建海. 针灸简易取穴法[M]. 广州: 科学普及出版社广州分社, 1988.
LI W X, XUE C L, LI J H. Simple acupuncture point selection [M]. Guangzhou: Science Popularization Press Guangzhou Branch, 1988.
YANG W T, HU H. Professor Hu Hui's clinical experience on the application of Baliao Point [J]. Journal of Beijing University of Chinese Medicine (Clinical Edition of Traditional Chinese Medicine), 2013, 20(1): 60-61.
KIM K C,CHO H C,JANG T J,et al. Automatic detection and segmentation of lumbar vertebrae from X-ray images for compression fracture evaluation[J]. Comput Methods Programs Biomed, 2021, 200: 105833.
FENG Q F,HOU Y H,HOU W G,et al. The effects of acupuncture on bladder interstitial cells of cajal excitability in rats with overactive bladder[J]. Evid Based Complement Alternat Med, 2013, 2013: 261217.
LI W, JIN Z, LIU J D, et al. Effect of electroacupuncture on expressionof p-ERK1/2 and c-fos in spinal dorsal horn in rats with interstitial cystitis [J]. Acupuncture Research, 21, 46(5) : 375-379.
YUAN Y H, ZHAO R X, XIA Q C, et al. The controversy about the location of experimental rats'acupoints —Taking Shenque as an example[J].Lishizhen Medicine and Materia Medica Research, 2022,33 (11) : 2714-2715.