Study on anti⁃inflammatory effect of components absorbed into blood of aqueous extract of "Curculiginis Rhizoma and Epimedii Folium"

AI Wenqi; ZHANG Xuesong; ZHENG Junzuo; XIE Yuman; WANG Ping; LAN Zhou

doi:10.16306/j.1008-861x.2024.02.005

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Study on anti⁃inflammatory effect of components absorbed into blood of aqueous extract of "Curculiginis Rhizoma and Epimedii Folium"

Special column on anti-inflammatory of traditional Chinese medicine | 更新时间：2024-04-15

- Study on anti⁃inflammatory effect of components absorbed into blood of aqueous extract of "Curculiginis Rhizoma and Epimedii Folium"
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 38, Issue 2, Pages: 29-35(2024)
- 作者机构：
  
  1.湖北中医药大学药学院（湖北　武汉　430065）
  2.湖北中医药大学基础医学院（湖北　武汉　430065）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2024.02.005
  CLC：
- Published：25 March 2024，
  
  Received：27 April 2023，
  
  Revised：27 May 2023，
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艾文琪,张学松,郑军佐等.仙茅⁃淫羊藿水提物入血成分的抗炎作用研究[J].上海中医药大学学报,2024,38(02):29-35.

AI Wenqi,ZHANG Xuesong,ZHENG Junzuo,et al.Study on anti⁃inflammatory effect of components absorbed into blood of aqueous extract of "Curculiginis Rhizoma and Epimedii Folium"[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2024,38(02):29-35.
艾文琪,张学松,郑军佐等.仙茅⁃淫羊藿水提物入血成分的抗炎作用研究[J].上海中医药大学学报,2024,38(02):29-35. DOI： 10.16306/j.1008-861x.2024.02.005.

AI Wenqi,ZHANG Xuesong,ZHENG Junzuo,et al.Study on anti⁃inflammatory effect of components absorbed into blood of aqueous extract of "Curculiginis Rhizoma and Epimedii Folium"[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2024,38(02):29-35. DOI： 10.16306/j.1008-861x.2024.02.005.

摘要

目的

探究仙茅-淫羊藿水提物（EX）中入血成分的抗炎作用。

方法

采用AutoDockTools软件将EX中5种入血成分（仙茅苷、淫羊藿苷、宝藿苷I、苔黑酚葡萄糖苷和朝藿定A）与靶蛋白髓细胞触发受体2（TREM2）进行分子对接；采用细胞计数试剂盒（CCK8）检测EX中5种入血成分在不同浓度下对小胶质细胞（BV2）细胞活力的影响；采用LPS刺激BV2或小鼠骨髓来源的巨噬细胞（BMDMs）及尼日利亚菌素（nigericin）刺激LPS预处理的BMDMs细胞建立炎症模型，并用酶联免疫吸附测定法（ELISA）检测5种入血成分对细胞上清液中肿瘤坏死因子-α（TNF-α）和白介素-1β（IL-1β）水平的影响。

结果

EX的入血成分仙茅苷、淫羊藿苷、宝藿苷I和朝藿定A与TREM2结合十分稳定，苔黑酚葡萄糖苷与TREM2结合较为稳定；仙茅苷、淫羊藿苷、宝藿苷I、朝藿定A和苔黑酚葡萄糖苷均可在一定浓度下减少BMDMs细胞产生的IL-1β水平，而仅高浓度的仙茅苷和淫羊藿苷可减少BMDMs细胞产生的TNF-α水平。

结论

EX的5种入血成分均可与TREM2结合，抑制下游NF-κB、NLRP3炎症通路改善炎症，可能是EX调控TREM2改善学习记忆能力和神经炎症的有效物质基础，在EX中起到协同增效的作用。

Abstract

Objective： To explore the anti-inflammatory effect of the components absorbed into serum of the aqueous extract of "Curculiginis Rhizoma and Epimedii Folium" （EX）.

Methods

AutoDockTools software was used to dock the five components absorbed into serum of EX （Curculigoside， Icariin， Baohuoside I， Oricinol glucoside and Epimedin A） with target protein TREM2. CCK8 method was used to detect the effects of the five components on BV2 cell viability at different concentrations. LPS was used to stimulate BV2 or mouse bone marrow-derived BMDMs cells and Nigericin to stimulate LPS pretreated BMDMs cells to establish inflammatory models， and the effects of the five components on TNF-α and IL-1β levels in the cell supernatant were detected by ELISA.

Results

The components absorbed into serum of EX including Curculigoside， Icariin， Baohuoside I and Epimedin A， bound very stably to TREM2， while the binding of Orcinol glucoside to TREM2 was relatively stable. Curculigoside， Icariin， Baohuoside I， Orcinol glucoside and Epimedin A all reduced the level of IL-1β produced by BMDMs at certain concentrations， while only high concentrations of Curculigoside and Icariin reduced the level of TNF-α produced by BMDMs.

Conclusion

5 components absorbed into serum of EX could all bind to TREM2 and inhibit downstream NF-κB or NLRP3 inflammatory pathways to improve inflammation， which might be an effective material basis for EX to regulate TREM2 to improve learning and memory ability and neuroinflammation， and work synergistically in EX.

关键词

仙茅-淫羊藿水提物TREM2分子对接炎症

Keywords

Curculiginis Rhizoma and Epimedii Foliumaqueous extractTREM2molecular dockinginflammation

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