Quality evaluation of wild and cultivated Paeoniae Radix Rubra from different origins based on fingerprint combined with stoichiometry methods

YU Jiahe; JIN Zhongxian; LIU Jinfeng; WEI Mei; CHEN Xiangdong; SHI Jinli

doi:10.16306/j.1008-861x.2023.05.004

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Quality evaluation of wild and cultivated Paeoniae Radix Rubra from different origins based on fingerprint combined with stoichiometry methods

Special column on Analysis of traditional Chinese medicine | 更新时间：2023-12-02

- Quality evaluation of wild and cultivated Paeoniae Radix Rubra from different origins based on fingerprint combined with stoichiometry methods
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 37, Issue 5, Pages: 31-38(2023)
- 作者机构：
  
  1.北京中医药大学中药学院（北京　102488）
  2.广东一方制药有限公司（广东　佛山　528000）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2023.05.004
  CLC：
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于佳禾,金重先,刘金凤等.基于指纹图谱结合化学计量学方法评价不同产地野生与栽培赤芍质量[J].上海中医药大学学报,2023,37(05):31-38.

YU Jiahe,JIN Zhongxian,LIU Jinfeng,et al.Quality evaluation of wild and cultivated Paeoniae Radix Rubra from different origins based on fingerprint combined with stoichiometry methods[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(05):31-38.
于佳禾,金重先,刘金凤等.基于指纹图谱结合化学计量学方法评价不同产地野生与栽培赤芍质量[J].上海中医药大学学报,2023,37(05):31-38. DOI： 10.16306/j.1008-861x.2023.05.004.

YU Jiahe,JIN Zhongxian,LIU Jinfeng,et al.Quality evaluation of wild and cultivated Paeoniae Radix Rubra from different origins based on fingerprint combined with stoichiometry methods[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(05):31-38. DOI： 10.16306/j.1008-861x.2023.05.004.

摘要

目的,2,建立不同产地野生与栽培赤芍的高效液相色谱（HPLC）指纹图谱并结合灰色关联-逼近理想解排序法（TOPSIS）分析方法评价其质量。,方法,2,采用Agilent ZORBAX SB C18（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，检测波长254 nm。,结果,2,建立30批不同产地野生与栽培赤芍的指纹图谱，相似度为0.821~0.989；共标定了15个共有峰，指认了没食子酸、羟基芍药苷、儿茶素、芍药内酯苷、芍药苷、没食子酸乙酯、没食子酰芍药苷、苯甲酸、1,2,3,4,6-五没食子酰葡萄糖、芍药新苷、苯甲酰芍药苷11种成分；热图聚类分析显示30批不同产地野生与栽培赤芍聚为3类；灰色关联度TOPSIS分析不同产地野生与栽培赤芍的质量，综合评价河北野生赤芍质量最优；河北栽培、四川栽培赤芍品质较优；黑龙江野生、内蒙古野生、吉林栽培、山东栽培、安徽栽培赤芍质量次之；黑龙江栽培、内蒙古栽培赤芍质量较差。提示河北、四川等区域可作为赤芍的适宜栽培产地。,结论,2,该结果可为赤芍药材的质量评价提供参考，同时也为赤芍栽培品的市场流通及种植选址提供依据。

Abstract

Objective： To establish high performance Liquid chromatography（HPLC） fingerprints of wild and cultivated Paeoniae Radix Rubra from different origins and to evaluate its quality by grey correlation-technique for order preference by similarity to an ideal solutiow（TOPSIS） analysis.,Methods,2,HPLC analysis was performed on Agilent ZORBAX SB-C18 column （4.6 mm×250 mm， 5 μm）， and the mobile phase was acetonitrile （A） - 0.1% phosphate acid （B） for gradient elution. The detection wavelength was 254 nm.,Results,2,HPLC fingerprints of 30 batches of wild and cultivated Paeoniae Radix Rubra from different origins were obtained， with similarity ranging from 0.821 to 0.989， from which 15 common peaks were calibrated and 11 components were identified， including gallic acid， hydroxypaeoniflorin， catechin， albiflorin， paeoniflorin， ethyl gallate， galloyl paeoniflorin， benzoic acid， 1,2,3,4,6-pentagaloglucose， lactiflorin， benzoyl paeoniflorin. Cluster heatmap analysis showed that the 30 batches of wild and cultivated Paeoniae Radix Rubra from different origins were clustered into three categories. The quality analysis of wild and cultivated Paeoniae Radix Rubra from different origins was performed by grey correlation-TOPSIS. Overall， Hebei wild sample ranked the first and had the best quality， the quality of Hebei cultivated sample and Sichuan cultivated sample is better than that of the left samples， Heilongjiang wild sample， Nei Mongol wild sample， Jilin cultivated sample， Shandong cultivated sample and Anhui cultivated sample ranked behind， Heilongjiang cultivated sample and Nei Mongol cultivated sample had lower ranking with the poor quality. It indicated the regions such as Hebei and Sichuan can be suitable cultivation areas for Paeoniae Radix Rubra.,Conclusion,2,This result can provide reference for quality evaluation of Paeoniae Radix Rubra and also provide the basis for market circulation and planting site selection of Paeoniae Radix Rubra.

关键词

赤芍野生品栽培品HPLC指纹图谱灰色关联-TOPSIS分析质量评价

Keywords

Paeoniae Radix Rubrawild samplecultivated sampleHPLC fingerprintgrey correlation-TOPSISquality evaluation

references

国家药典委员会. 中华人民共和国药典（2020版）：一部［S］. 北京：中国医药科技出版社， 2020： 165. Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China （2020 Edition）： Volume 1［S］. Beijing： China Medical Science Press， 2020： 165.

LIU E H， QI L W， LI B， et al. High-speed separation and characterization of major constituents in Radix Paeoniae Rubra by fast high-performance liquid chromatography coupled with diode-array detection and time-of-flight mass spectrometry［J］. Rapid Commun Mass Sp， 2009， 23（1）： 119-30.

卜璟. 赤芍降血糖有效部位化学成分研究［D］. 北京：中国中医科学院， 2013.

BU J. Chemical Basis Research on Hpyerglycemic Effective Part of Radix Paeoniae Rubra［D］. Beijing：China Academy of Chinese Medical Sciences， 2013.

吴玲芳，王子墨，赫柯芊，等. 赤芍的化学成分和药理作用研究概况［J］. 中国实验方剂学杂志， 2021， 27（18）： 198-206.

WU L F， WANG Z M， HE K Q， et al. chemical constituents and pharmacological effects of paeoniae radix rubra： a review［J］. Chinese Journal of Experimental Traditional Medical Formulae， 2021， 27（18）： 198-206.

LU Z， YE Y， LIU Y， et al. Aqueous extract of Paeoniae Radix Rubra prevents deep vein thrombosis by ameliorating inflammation through inhibiting GSK3β activity［J］. Phytomedicine. 2021， 92： 153767.

XIE P， CUI L， SHAN Y， et al. Antithrombotic Effect and Mechanism of Radix Paeoniae Rubra［J］. Biomed Res Int， 2017， 2017： 9475074.

刘福芳. 呼伦贝尔野生赤芍资源科学保护与开发的途径［J］. 呼伦贝尔学院学报， 2021， 29（2）： 68-70.

LIU F F. Scientific Development and Protection of Wild Paeoniae Radix Rubra Resources in Hulunbuir［J］. Journal of Hulunbeier Universities， 2021， 29（2）： 68-70.

杨昌林. 赤芍商品药材调查及品质评价研究［D］. 成都：成都中医药大学， 2011.

YANG C L. Studies on the Commodity Survey and Quality Evaluation of Radix Paeonia Rubra［D］. Chengdu：Chengdu University of Traditional Chinese Medicine， 2011.

谢婷，周萌，李鑫，等. 多伦赤芍野生植株和芽头无性栽培植株生物学性状和芍药苷含量对比研究［J］. 中国中药杂志， 2015， 40（24）： 4830-4833.

XIE T， ZHOU M， LI X， et al. Comparative study of biological characters and paeoniflorin content of wild and asexual cultivated Paeonia lactiflora growing at Duolun county， Inner Mongolia［J］. China Journal of Chinese Materia Medica， 2015， 40（24）： 4830-4833.

胡媛，赵梓辰，杨昌林，等. 野生赤芍和栽培芍药的芍药苷、苯甲酸含量测定［J］. 中药与临床， 2014， 5（6）： 3-5，12.

HU Y， ZHAO Z C， YANG C L， et al. The determination of paeoniflorin and benzoic acid in the wild and cultivated Radix Paeoniae Rubra［J］. Pharmacy and Clinics of Chinese Materia Medica， 2014，5（6）： 3-5，12.

俞敬波，冯学锋，王文全，等. 不同产地赤芍野生品与栽培品性状显微特征比较研究［J］. 中国中药杂志， 2010， 35（19）： 2533-2537.

YU J B， FENG X F， WANG W Q， et al. Comparative research on pharmacognostic characteristics and microscopic characteristics of Radix paeoniae rubra from different areas［J］. China Journal of Chinese Materia Medica， 2010， 35（19）： 2533-2537.

金顺琪，张露蓉，李曼，等. 鲜半枝莲高效液相色谱指纹图谱的建立及10种黄酮类成分含量测定［J］. 中国医院药学杂志， 2022， 42（2）： 119-122.

JIN S Q， ZHANG L R， LI M， et al. Establishment of HPLC fingerprints of Fresh Scutellaria barbataand determination of 10 flavonoids components［J］. Chinese Journal of Hospital Pharmacy， 2022， 42（2）： 119-122.

丁永胜，罗静，赵程博文，等. 基于HPLC法和灰色关联法评价不同产地、不同商品规格三七质量［J］. 中草药， 2020， 51（4）： 1069-1075.

DING Y S， LUO J， ZHAO C B W， et al. Quality evaluation of different origins and commercial grades of Panax notginseng by HPLC and grey correlation analysis［J］. Chinese Herbal Medicines， 2020， 51（4）： 1069-1075.

马转霞，孙岚萍，顾志荣等.基于灰色关联度分析与TOPSIS模型的不同产地锁阳综合质量评价［J］. 中成药， 2020， 42（10）： 2680-2686.

MA Z X， SUN L P， GU Z R， et al. Comprehensive quality evaluation of Cynomorium songaricum from different growing areas based on grey correlation analysis and TOPSIS model［J］. Chinese Traditional Patent Medicine， 2020， 42（10）： 2680-2686.

于江泳，黄绮韵，周苏娟，等. 基于灰色关联-TOPSIS法的干姜质量评价［J］.中国实验方剂学杂志， 2016， 22（18）： 56-59.

YU J Y， HUANG Q Y， ZHOU S J， et al. Application of Grey Correlation-TOPSIS Method in Quality Evaluation of Zingiberis Rhizoma［J］. Chinese Journal of Experimental Traditional Medical Formulae， 2016， 22（18）： 56-59.

付士朋，沈宏伟，王谦博，等. 指纹图谱结合灰色关联度分析法对不同产地赤芍质量的评价研究［J］. 中草药， 2019， 50（23）： 5865-5871.

FU S P， SHEN H W， WANG Q B， et al. Study on fingerprint and gray correlation analysis of Paeoniae Radix Rubra from different habitats［J］. Chinese Herbal Medicines， 2019， 50（23）： 5865-5871.

孙冬梅，陈秋谷，毕晓黎，等. 二基原赤芍HPLC指纹图谱的建立和5种成分的含量测定［J］. 中药材， 2018， 41（4）： 898-903.

SUN D M， CHEN Q G， BI X L， et al. Fingerprint and Five Constituents Quantitative Determination for Paeoniae Radix Rubra from Second Origins by HPLC［J］. Journal of Chinese Medicinal Materials， 2018， 41（4）： 898-903.

贾银芝，吴琦，杨绪芳，等. 基于灰色关联-TOPSIS法评价不同产地山楂质量［J］. 中草药， 2021， 52（23）： 7317-7322.

JIA Y Z， WU Q， YANG X F， et al. Quality evaluation of Crataegi Fructus from different habitats based on grey correlation-TOPSIS method［J］. Chinese Herbal Medicines， 2021， 52（23）： 7317-7322.

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College of Pharmaceutical Sciences， Shanghai Jiao Tong University

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