Study on NMR determination of momordica saponin Ⅰ

WANG Jiaqi; WU Ziwei; SHI Xiaoying; LI Xiaobo; WANG Mengyue

doi:10.16306/j.1008-861x.2023.03.008

您当前的位置：

首页 >

文章列表页 >

Study on NMR determination of momordica saponin Ⅰ

Experiment Research | 更新时间：2023-06-03

- Study on NMR determination of momordica saponin Ⅰ
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 37, Issue 3, Pages: 42-47(2023)
- 作者机构：
  
  1.上海交通大学药学院（上海　200240）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2023.03.008
  CLC：
扫描看全文
王嘉祺,吴子薇,时晓颖等.木鳖子苷Ⅰ的NMR测定研究[J].上海中医药大学学报,2023,37(03):42-47.

WANG Jiaqi,WU Ziwei,SHI Xiaoying,et al.Study on NMR determination of momordica saponin Ⅰ[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(03):42-47.
王嘉祺,吴子薇,时晓颖等.木鳖子苷Ⅰ的NMR测定研究[J].上海中医药大学学报,2023,37(03):42-47. DOI： 10.16306/j.1008-861x.2023.03.008.

WANG Jiaqi,WU Ziwei,SHI Xiaoying,et al.Study on NMR determination of momordica saponin Ⅰ[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(03):42-47. DOI： 10.16306/j.1008-861x.2023.03.008.

摘要

目的,2,研究木鳖子苷Ⅰ的化学结构及纯度测定方法。,方法,2,采用2D NMR技术、水解方法确定化学结构及NMR数据归属；采用,1,H-NMR方法测定纯度。,结果,2,运用2D NMR技术，结合酸水解、碱水解方法，快速确定了化学结构，首次完成了木鳖子苷Ⅰ NMR数据全归属。建立了,1,H-NMR方法测定木鳖子苷Ⅰ的纯度，该方法不需要特殊对照品，操作简单、快速准确。,结论,2,本研究为复杂皂苷类成分的结构及纯度测定提供了方法借鉴。

Abstract

Objective： To investigate the chemical structure and purity determination method of momordica saponin Ⅰ （MSⅠ）.,Methods,2,2D NMR technology and hydrolysis method were used to determine the chemical structure and assign the NMR data. ,1,H-NMR technology was used to determine the purity.,Results,2,The chemical structure of MSⅠ was rapidly determined and the NMR data was completely assigned for the first time by 2D NMR technology combined with acid and alkaline hydrolysis. A simple， rapid， and accurate method for the purity determination of MSⅠ was established by ,1,H-NMR which didn’t need specific reference substance.,Conclusion,2,The study provided a methodological reference for the chemical structure and purity determination of complex saponins.

关键词

木鳖子苷Ⅰ木鳖子NMR信号归属纯度测定

Keywords

momordica saponin Ⅰmomordicae SemenNMRdata assignmentpurity determination

references

国家药典委员会. 中华人民共和国药典（一部）［S］. 北京：中国医药科技出版社， 2020： 66-67.

Chinese Pharmacopeia Committee. Chinese Pharmacopeia （Vol Ⅰ）［S］. Beijing： China Medical Science and Technology Press， 2020： 66-67.

赵芳惠，侯小涛，郝二伟，等. 木鳖子化学成分、毒理与药理作用研究进展［J］. 中国实验方剂学杂志， 2020， 26 （3）： 222-235.

ZHAO F H， HOU X T， HAO E W， et al. Chemical constituents， toxicology and pharmacological effect of Momordicae Semen ［J］. Chinese Journal of Experimental Traditional Medical Formulae， 2020， 26 （3）： 222-235.

LAN H Y， ZHAO B， SHEN Y L， et al. Phytochemistry， pharmacological activities， toxicity and clinical application of Momordica cochinchinensis ［J］. Curr Pharm Design， 2019， 25 （6）： 715-728.

WANG M Y， ZHAN Z B， XIONG Y， et al. Cytotoxic and anti-inflammatory constituents from Momordica cochinchinensis seeds ［J］. Fitoterapia， 2019， 139： 104360.

YU J S ， KIM J H， LEE S， et al. Src/Syk-targeted anti-inflammatory actions of triterpenoidal saponins from gac （Momordica cochinchinensis） seeds ［J］. Am J Chinese Med， 2017， 45 （3）： 459-473.

WANG P F， DING L， KIM H， et al. Vaccine adjuvants derivatized from momordica saponins I and II ［J］. J Med Chem， 2019， 62 （21）： 9976-9982.

JUNG K， LEE D， YU J S， et al. Protective effect and mechanism of action of saponins isolated from the seeds of gac （Momordica cochinchinensis Spreng.） against cisplatin-induced damage in LLC-PK1 kidney cells ［J］. Bioorg Med Chem Lett， 2016， 26 （5）： 1466-1470.

IWAMOTO M， HIKARU O， YAMAUCHI T， et al. Studies on the constituents of Momordica cochinchinensis Spreng. I. Isolation and characterization of the seed saponins， momordica saponins I and Ⅱ ［J］. Chem Pharm Bull， 1985， 33 （2）： 464-478.

Views

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰