Application and advances of UDP⁃sugar donors in biosynthesis of glycosides

LU Xinxin; NIU Tengfei; ZHAO Shujuan; WANG Zhengtao; WANG Rufeng

doi:10.16306/j.1008-861x.2023.02.013

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Application and advances of UDP⁃sugar donors in biosynthesis of glycosides

Review | 更新时间：2023-03-24

- Application and advances of UDP⁃sugar donors in biosynthesis of glycosides
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 37, Issue 2, Pages: 85-95(2023)
- 作者机构：
  
  1.上海中医药大学中药研究所，中药标准化教育部重点实验室，上海中药标准化研究中心（上海　201203）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2023.02.013
  CLC：
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卢欣欣,牛腾飞,赵淑娟等.UDP⁃糖基供体在中药苷类成分生物合成中的应用及研究进展[J].上海中医药大学学报,2023,37(02):85-95.

LU Xinxin,NIU Tengfei,ZHAO Shujuan,et al.Application and advances of UDP⁃sugar donors in biosynthesis of glycosides[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(02):85-95.
卢欣欣,牛腾飞,赵淑娟等.UDP⁃糖基供体在中药苷类成分生物合成中的应用及研究进展[J].上海中医药大学学报,2023,37(02):85-95. DOI： 10.16306/j.1008-861x.2023.02.013.

LU Xinxin,NIU Tengfei,ZHAO Shujuan,et al.Application and advances of UDP⁃sugar donors in biosynthesis of glycosides[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2023,37(02):85-95. DOI： 10.16306/j.1008-861x.2023.02.013.

摘要

尿苷二磷酸（UDP）-糖基供体是三萜、黄酮和蒽醌等中药活性成分糖基化修饰的重要组成前体。近年来，通过代谢工程策略合成苷类化合物已取得一定进展，但UDP-糖基供体在不同类型的底盘细胞中产量普遍偏低，极大地限制了中药苷类化合物的高效生物合成。作者从UDP-糖基供体的生物合成、循环再生以及代谢合成途径中关键基因调控、代谢模块优化等方面，重点综述了UDP-糖基供体在苷类成分合成中供应的研究进展，并展望了UDP-糖基供体在生物合成中的挑战和发展方向，以期为今后苷类成分的生物合成相关研究提供借鉴。

Abstract

Uridine diphosphate （UDP）-sugar donors are key precursors for glycosylation modification of active ingredients of traditional Chinese medicine， such as triterpenoids， flavonoids， and anthraquinones. In recent years， some progress has been made in the synthesis of glycosides by metabolic engineering strategies， but the low yield of UDP-sugar donors in various chassis cells limits the efficient biosynthesis of traditional Chinese medicine glycosides. In this review， the research progress in the supply of UDP-sugar donors in the synthesis of glycosides were reviewed from the aspects of biosynthesis， recycling and regeneration of UDP-sugar donors as well as the regulation of key genes in the metabolic synthesis pathway and optimization of metabolic modules. Furthermore， research challenges and development directions of UDP-sugar donors in glycosides biosynthesis were discussed， in order to provide a reference for further research on glycoside biosynthesis.

关键词

UDP-糖基供体苷类化合物代谢调控生物合成

Keywords

UDP-sugar donorsglycosidesmetabolic regulationbiosynthesis

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