Objective： A rapid quantitative detection method for 12 chemical constituents in different genera of Celastraceae was established by ultra-high-performance liquid-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry （UHPLC-Q/Exactive）.,Methods,2,Different genera of Celastraceae are crushed， sieved， and extracted with methanol ultrasonically. UPLC,®,HSS T3 column （2.1 mm×100 mm， 1.8 μm） was used for gradient elution with the mobile phase consists of 0.1% formic acid aqueous solution as the aqueous phase and acetonitrile as the organic phase， and the detection was performed with quadrupole/electrostatic field orbitrap high-resolution mass spectrometry with electrospray ion source in positive ion mode， and internal standard method is used for quantification.,Results,2,The method had good linearity and wide linear range. The 12 chemical components all had good linear relationship in the range of 0.8 ~ 2 000 ng/ml， ,R,2,>,0.99. The sample spiked recoveries were 97.1%~108.0%， and the relative standard deviations were 0.1%~1.2%.,Conclusion,2,This method has the advantages of rapidness， simple operation and high accuracy. It can be used for the rapid quantitative analysis of the constituents of Celastraceae， which can better promote the rational use of Celastraceae expand the available classes of herbs， and provide a certain basis for scientific development of Chinese medicine resources.