Exploring potential mechanism of Erxian Decoction on mammary hyperplasia based on network pharmacology

Nannan DU; Jiaye SUN; Jiamei FENG; Xueqing WU

doi:10.16306/j.1008-861x.2022.S1.046

您当前的位置：

首页 >

文章列表页 >

Exploring potential mechanism of Erxian Decoction on mammary hyperplasia based on network pharmacology

Experiment Research | 更新时间：2022-08-29

- Exploring potential mechanism of Erxian Decoction on mammary hyperplasia based on network pharmacology
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue S1, Pages: 220-226(2022)
- 作者机构：
  
  1.上海中医药大学附属曙光医院乳腺科（上海　200021）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.S1.046
  CLC：
扫描看全文
杜楠楠,孙佳晔,冯佳梅等.基于网络药理学探讨二仙汤治疗乳腺增生症的潜在作用机制[J].上海中医药大学学报,2022,36(S1):220-226.

DU Nannan,SUN Jiaye,FENG Jiamei,et al.Exploring potential mechanism of Erxian Decoction on mammary hyperplasia based on network pharmacology[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):220-226.
杜楠楠,孙佳晔,冯佳梅等.基于网络药理学探讨二仙汤治疗乳腺增生症的潜在作用机制[J].上海中医药大学学报,2022,36(S1):220-226. DOI： 10.16306/j.1008-861x.2022.S1.046.

DU Nannan,SUN Jiaye,FENG Jiamei,et al.Exploring potential mechanism of Erxian Decoction on mammary hyperplasia based on network pharmacology[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):220-226. DOI： 10.16306/j.1008-861x.2022.S1.046.

摘要

目的,2,运用网络药理学的研究方法，分析二仙汤治疗乳腺增生症的潜在作用靶点和机制。,方法,2,使用中药系统药理学数据库与分析平台（TCMSP）筛选二仙汤的有效活性成分及其作用靶点，利用GeneCards数据库检索乳腺增生症的相关靶点。映射疾病靶点与药物靶点，通过String数据库预测两者间关键靶点的相互作用，构建“药物-有效成分-作用靶点”网络图，并对关键靶点进行基因本体（GO）富集分析和京都基因和基因组百科全书（KEGG）富集分析，分析二仙汤治疗乳腺增生症的潜在作用机制。,结果,2,共获得85个二仙汤有效成分，215个有效成分靶点，4 775个乳腺增生症靶点，有效成分与疾病交集靶点175个，蛋白质相互作用（PPI）核心网络包含10个靶点，并得出排名前10位的GO富集结果和排名前20位的KEGG富集结果。,结论,2,二仙汤通过槲皮素、山柰酚、β-谷甾醇等多个活性成分作用于,ESR1,、,TP53,、,AKT1,等靶点，直接或间接通过调控PI3K-AKT、MAPK等多条信号通路调节雌激素的活性，发挥治疗乳腺增生症的作用。

Abstract

Objective： To analyze the potential targets and mechanisms of Erxian Decoction on mammary hyperplasia by network pharmacology.,Methods,2,The Chinese Medicine System Pharmacology Database and Analysis Platform （TCMSP） was used to screen the active ingredients of Erxian Decoction and their targets. Then the GeneCards database was used to search for the relevant targets of mammary hyperplasia. Map disease targets and drug targets， and predict the interactions of key targets between them through String database. Construct the network diagram of “drug-active component-action target”. And key targets were analyzed by Gene Ontology （GO） enrichment analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis， to analyze the potential mechanism of Erxian Decoction on mammary hyperplasia.,Results,2,A total of 85 active ingredients of Erxian Decoction， 215 targets of active ingredient， 4 775 targets of mammary hyperplasia， 175 intersection targets of active ingredients and diseases were obtained. The protein-protein interaction （PPI） core network contains 10 targets， and the top 10 GO enrichment results and the top 20 KEGG enrichment results were obtained.,Conclusion,2,Erxian Decoction acts on ,ESR1,， ,TP53,， ,AKT1, and other targets through quercetin， kaherol， β -sitosterol and other active ingredients， and directly or indirectly regulates the activity of estrogen by regulating PI3K-AKT， MAPK and other signal pathways， thus exerts therapeutic effects of treating mammary gland hyperplasia.

关键词

二仙汤乳腺增生症雌激素网络药理学

Keywords

Erxian Decoctionmammary hyperplasiaestrogennetwork pharmacology

references

MANSEL R E， Webster D J， SWEETLAND H M. Hughes， Mansel & Webster’s Benign Disorders and Diseases of the Breast［M］. London： Bailliere Tindal， 2009： 1-4.

马薇，金泉秀，吴云飞，等. 乳腺增生症诊治专家共识［J］. 中国实用外科杂志，2016， 36（7）： 759-762.

陶仕英，牛建昭，王继峰，等. 二仙汤及其组方中药对幼年大鼠肾上腺雌激素受体表达的影响［J］. 中国中医药信息杂志，2010， 17（5）： 36-37.

魏青，周秀芳. 中西医治疗乳腺增生症的研究进展［J］.内蒙古医科大学学报，2021， 43（4）： 434-437.

VIJAYANARAYANA K， RODRIGUES R S，CHANDRASHEK-HAR K S，et al.Evaluation of estrogenic activity of alcoholic extract of rhizomes of Curculigo orchioides［J］. J Ethnopharmacol， 2007， 114（2）： 241-245.

CVORO A，PARUTHIYIL S， JONES J O， et al. Selective activation of estrogen receptor-beta transcriptional pathways by an herbal extract［J］. Endocrinology， 2007， 148（2）： 538-547.

CIRCOSTA C， PASQUALE R D， PALUMBO D R， et al.Estrogenic activity of standardized extract of Angelica sinensis［J］. Phytother Res， 2006， 20（8）： 665-669.

KANG H K， CHOI Y H， KWON H， et al. Estrogenic/antiestrogenic activities of a Epimedium koreanum extract and its major components： in vitro and in vivo studies［J］. Food Chem Toxicol， 2012， 50（8）： 2751-2759.

GUO Y， ZHANG X， MENG J， et al.An anticancer agent icaritin induces sustained activation of the extracellular signal-regulated kinase （ERK） pathway and inhibits growth of breast cancer cells［J］. Eur J Pharmacol. 2011， 658（2-3）： 114-122.

KENDALL C W， KOO M， SOKOLOFF E， et al. Effect of dietary oxidized cholesterol on azoxymethane-induced colonic preneoplasia in mice［J］. Cancer Lett， 1992， 66（3）： 241-248.

MACON M B， FENTON S E. Endocrine disruptors and the breast： early life effects and later life disease［J］. J Mammary Gland Biol Neoplasia， 2013， 18（1）： 43-61.

LI X， XIN P， WANG C， et al.Mechanisms of Traditional Chinese Medicine in the Treatment of Mammary Gland Hyperplasia［J］. Am J Chin Med， 2017， 45（3）： 443-458.

李炜，牛建昭，王继峰，等. 槲皮素的植物雌激素作用及其受体机制研究［J］.中国医院药学杂志，2015， 35（2）： 91-95.

杨英，曹阳，张婷婷，等. 槲皮素药理作用在雌激素相关性疾病中的研究进展［J］. 国际生殖健康/计划生育杂志，2011， 30（1）： 69-72.

LEHMANN L， JIANG L， WAGNER J. Soy isoflavones decrease the catechol-O-methyltransferase-mediated inactivation of 4-hydroxyestradiol in cultured MCF-7 cells［J］.Carcinogenesis， 2008， 29（2）： 363-370.

SOTOCA A M， RATMAN D， GUSTAFSSON J A， et al.Phytoestrogen-mediated inhibition of proliferation of the human T47D breast cancer cells depends on the ERalpha/ERbeta ratio［J］. J Steroid Biochem Mol Biol， 2008， 112（4-5）： 171-178.

PADMA V V， LALITHA G， SHIRONY N P， et al.Effect of quercetin against lindane induced alterations in the serum and hepatic tissue lipids in wistar rats［J］. Asian Pac J Trop Biomed， 2012， 2（11）： 910-915.

ZHANG M， XIE Z， GAO W，et al. Quercetin regulates hepatic cholesterol metabolism by promoting cholesterol-to-bile acid conversion and cholesterol efflux in rats［J］. Nutr Res， 2016， 36（3）： 271-279.

KLIMBERG V S. Etiology and management of breast pain，partII： Copel and EM， Bland KI，editors. The breast［M］. PHiladelPHia： WB Saunders Co， 1998： 247-260.

WU Y， ZHANG Q， ZHANG R.Kaempferol targets estrogen-related receptor α and suppresses the angiogenesis of human retinal endothelial cells under high glucose conditions［J］. Exp Ther Med， 2017， 14（6）： 5576-5582.

TOH M F， MENDONCA E， EDDIE S L， et al.Kaempferol Exhibits Progestogenic Effects in Ovariectomized Rats［J］. J Steroids Horm Sci， 2014， 5（3）： 136.

JU Y H， CLAUSEN L M， ALLRED K F， et al. beta-Sitosterol， beta-Sitosterol Glucoside， and a Mixture of beta-Sitosterol and beta-Sitosterol Glucoside Modulate the Growth of Estrogen-Responsive Breast Cancer Cells In Vitro and in Ovariectomized Athymic Mice［J］. J Nutr， 2004， 134（5）： 1145-1151.

ROSENBLAT M， VOILOVA N， AVIRAM M.Pomegranate phytosterol （β-sitosterol） and polyphenolic antioxidant （punicalagin） addition to statin， significantly protected against macrophage foam cells formation［J］. Atherosclerosis， 2013， 226（1）： 110-117.

FENG S， DAI Z， LIU A B， et al.Intake of stigmasterol and β-sitosterol alters lipid metabolism and alleviates NAFLD in mice fed a high-fat western-style diet［J］. Biochim Biophys Acta Mol Cell Biol Lipids， 2018， 1863（10）： 1274-1284.

林妮，邱玉文，何贡云，等. 荔枝核皂苷对大鼠乳腺增生的作用及对雌激素信号通路的影响［J］.中药材，38（4）： 798-802.

SOTOCA A M， RATMAN D， VERVOORT J， et al.Phytoestrogen-mediated inhibition of proliferation of the human T47D breast cancer cells depends on the ERalpha/ERbeta ratio［J］. J Steroid Biochem Mol Biol， 2008， 112（4-5）： 171-178.

SAMOLI E， TRICHOPOULOS D， LAGIOU A， et al.The hormonal profile of benign breast disease［J］. Br J Cancer， 2013， 108（1）： 199-204.

ARCHER S G， ELIOPOULOS A， SPANDIDOS D， et al.Expression of ras p21， p53 and c-erbB-2 in advanced breast cancer and response to first line hormonal therapy［J］. Br J Cancer， 1995， 72（5）： 1259-1266.

姜军，陈意生，马宏敏，等. p53蛋白在乳腺不典型增生和乳腺癌中的表达［J］. 第三军医大学学报，1998， 19（1）： 63-64.

李欣，林明哲，赵久达. 槲皮素对胃癌相关p53/AMPK/mTOR信号通路的影响［J］. 天津医药，2021， 49（11）： 1143-1147.

MANCINI M L， LIEN E C， TOKER A.Oncogenic AKT1（E17K） mutation induces mammary hyperplasia but prevents HER2-driven tumorigenesis［J］. Oncotarget， 2016， 14： 17301-17313.

LI X， ZHOU N， WANG J， et al.Quercetin suppresses breast cancer stem cells （CD44+/CD24-） by inhibiting the PI3K/Akt/mTOR-signaling pathway［J］. Life Sci， 2018， 196： 56-62.

YAMNIK R L， HOLZ M K. mTOR/S6K1 and MAPK/RSK signaling pathways coordinately regulate estrogen receptor alpha serine 167 phosphorylation［J］. FEBS Lett， 2010， 584（1）： 124-128.

AWAD A B， WILLIAMS H， FINK C S. Effect of phytosterols on cholesterol metabolism and MAP kinase in MDA-MB-231 human breast cancer cells［J］. J Nutr Biochem， 2003， 14（2）： 111-119.

Views

308

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Exploration on mechanism of Baitouweng Decoction against colorectal adenoma based on network pharmacology and experimental validation

Exploration on molecular mechanism of Fructus Psoraleae in treating lung adenocarcinoma based on network pharmacology and experimental pharmacology

Exploration on mechanism of Wuling Powder combined with Taohong Siwu Decoction in treatment of heart failure based on network pharmacology， molecular docking， and experimental validation

Literature mining and bioinformatics analysis of Xue Xue’s Medical Cases for differentiation and treatment of gastrointestinal diseases

Exploration on mechanism of Shaoyao Gancao Decoction in treating ulcerative colitis based on network pharmacology and in vitro cell experiments

Related Author

No data

Related Institution

Institute of Digestive Diseases， Longhua Hospital， Shanghai University of Traditional Chinese Medicine

Shanghai Pudong New Area Hospital of Traditional Chinese Medicine

Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Shanghai Municipal Hospital of Traditional Chinese Medicine， Shanghai University of Traditional Chinese Medicine

Institute of Interdisciplinary Integrative Medicine Research， Shanghai University of Traditional Chinese Medicine

⁰