Exploring mechanism of Fukangling on triple negative breast cancer based on network pharmacology

Yuzhu ZHANG; Tian LI; Runwei TANG; Shuai YUAN; Qiuyang YU; Weihong ZHANG

doi:10.16306/j.1008-861x.2022.S1.045

您当前的位置：

首页 >

文章列表页 >

Exploring mechanism of Fukangling on triple negative breast cancer based on network pharmacology

Experiment Research | 更新时间：2022-08-29

- Exploring mechanism of Fukangling on triple negative breast cancer based on network pharmacology
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue S1, Pages: 210-219(2022)
- 作者机构：
  
  1.上海中医药大学附属曙光医院宝山分院（上海　201999）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.S1.045
  CLC：
扫描看全文
张玉柱,李甜,唐润薇等.基于网络药理学探讨复康灵治疗三阴性乳腺癌的作用机制[J].上海中医药大学学报,2022,36(S1):210-219.

ZHANG Yuzhu,LI Tian,TANG Runwei,et al.Exploring mechanism of Fukangling on triple negative breast cancer based on network pharmacology[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):210-219.
张玉柱,李甜,唐润薇等.基于网络药理学探讨复康灵治疗三阴性乳腺癌的作用机制[J].上海中医药大学学报,2022,36(S1):210-219. DOI： 10.16306/j.1008-861x.2022.S1.045.

ZHANG Yuzhu,LI Tian,TANG Runwei,et al.Exploring mechanism of Fukangling on triple negative breast cancer based on network pharmacology[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):210-219. DOI： 10.16306/j.1008-861x.2022.S1.045.

摘要

目的,2,运用网络药理学方法筛选中药复方复康灵的活性成分以及作用于三阴性乳腺癌干细胞的靶点和通路，探讨其分子机制。,方法,2,通过TCMSP数据库、TCMID数据库和BATMAN-TCM 数据库筛选出复康灵活性成分及其相关靶标；通过GeneCards数据库和GEO数据库收集疾病相关基因，并做交集筛选目标基因；运用String数据库构建蛋白相互作用（PPI）网络；采用DAVID数据库对复康灵关键作用靶点进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析。,结果,2,通过筛选得到复康灵有效成分108个和对应的靶基因446个，与三阴性乳腺癌干细胞的交集基因为49个；GO分析结果显示复康灵活性成分的基因功能主要表现在信号调控、细胞刺激反应、氧化应激反应、细胞凋亡、炎症反应以及对细胞因子刺激的反应等生物过程中，其抗三阴性乳腺癌的复发和转移的作用主要是通过调控细胞增殖、迁移等生物过程；KEGG分析结果显示其主要通过调控肿瘤坏死因子（TNF）信号通路、丝裂原活化蛋白激酶（MAPK）信号通路、核转录因子-κB（NF-κB）信号通路、肿瘤通路、表皮生长因子受体（EGFR）酪氨酸激酶抑制剂耐药等发挥治疗作用。,结论,2,预测了复康灵对三阴性乳腺癌干细胞的作用靶点、物质基础和分子机制，以及复康灵对三阴性乳腺癌转移的分子机制。

Abstract

Objective： The network pharmacology was used to screen the active components of Fukangling and their targets and pathways on triple negative breast cancer stem cells， and explore the molecular mechanism.,Methods,2,TCMSP database， TCMID database and BATMAN-TCM database were used for screening active components of Fukangling and related targets. Disease-related genes were collected through GeneCards database and GEO database， and target genes were obtained by intersection. The protein interaction （PPI） network was constructed using String database. The key targets of Fukangling were analyzed by Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） using DAVID database.,Results,2,108 active ingredients of Fukangling and 446 corresponding target genes were obtained， and there were 49 genes associated with triple negative breast cancer stem cells. GO analysis showed that the gene function of Fukangling active compounds mainly manifested in biological progresses such as signal regulation， cell stimulation response， oxidative stress response， apoptosis， inflammatory response， and response to cytokine stimulation. Its anti-recurrence and metastasis against triple negative breast cancer is mainly through biological processes like regulating cell proliferation and migration. KEGG analysis showed that it mainly exerts therapeutic effects by regulating tumor necrosis factor （TNF） signaling pathway， mitogen-activated protein kinase （MAPK） signaling pathway， nuclear transcription factor-κB （NF-κB） signaling pathway， tumor pathway， epidermal growth factor receptor （EGFR） tyrosine kinase inhibitor resistance and so on.,Conclusion,2,The targets， material basis and molecular mechanism of Fukangling on breast cancer stem cells， and its molecular mechanism were predicted.

关键词

复康灵三阴性乳腺癌干细胞网络药理学通路分子机制

Keywords

Fukanglingtriple negative breast cancer stem cellsnetwork pharmacologypathwaymolecular mechanism

references

SIEGEL R L， MILLER K D， FUCHS H E， et al. Cancer statistics， 2022［J］. CA Cancer J Clin， 2022， 72（1）： 7-33.

BELLI C，DUSO B A，FERRARO E，et al.Homologous recombination deficiency in triple negative breast cancer ［J］.Breast， 2019， 45： 15-21.

VERIGOS J，KARAKAIDOS P，KORDIAS D，et al.The Histone Demethylase LSD1/KappaDM1A Mediates Chemoresistance in Breast Cancer via Regulation of a Stem Cell Program ［J］. Cancers （Basel），2019， 11（10）： 1585.

苏菲，万冬桂，刘芳，等.中医药防治三阴乳腺癌研究进展［J］.中国中医基础医学杂志，2018， 24（11）： 1649-1651.

李朵璐，柴玉娜，王松鹏.益气健脾法在三阴性乳腺癌治疗中的作用研究进展［J］.中医研究，2019， 32（2）： 74-78.

谢宛君，林毅，梁倩蓉，等.基于数据挖掘的林毅教授转移性乳腺癌论治经验［J］.中国中药杂志，2018， 43（15）： 3198-3204.

梁倩蓉，陈前军.基于数据挖掘技术分析林毅教授治疗乳腺癌的用药规律［J］.中医药导报，2018， 24（15）： 36-39.

TIAN G，WU C，LI J，et al.Network pharmacology based investigation into the effect and mechanism of Modified Sijunzi Decoction against the subtypes of chronic atrophic gastritis ［J］.Pharmacol Res， 2019， 144： 158-166.

RU J， LI P， WANG J，et al.TCMSP：a database of systems pharmacology for drug discovery from herbal medicines ［J］. J Cheminform， 2014， 6： 13.

XUE R，FANG Z，ZHANG M，et al.TCMID：Traditional Chinese Medicine integrative database for herb molecular mechanism analysis ［J］. Nucleic Acids Res， 2013， 41（Database issue）：D1089-D1095.

LIU Z，GUO F，WANG Y，et al.BATMAN-TCM：a Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine ［J］. Sci Rep， 2016， 6： 21146.

QIN S，YIN J，HUANG S，et al.Astragaloside IV Protects Ethanol-Induced Gastric Mucosal Injury by Preventing Mitochondrial Oxidative Stress and the Activation of Mitochondrial Pathway Apoptosis in Rats ［J］. Front Pharmacol，2019， 10： 894.

SAFRAN M，DALAH I，ALEXANDER J，et al.GeneCards Version 3：the human gene integrator ［J］.Database （Oxford），2010， 2010： baq020.

BARRETT T，TROUP D B，WILHITE S E，et al.NCBI GEO：archive for high-throughput functional genomic data ［J］. Nucleic Acids Res， 2009， 37（Database issue）： D885-D890.

CHEN Q， YU D， ZHAO Y， et al.Screening and identification of hub genes in pancreatic cancer by integrated bioinformatics analysis ［J］. J Cell Biochem， 2019， 120（12）： 19496-19508.

SZKLARCZYK D，GABLE A L，LYON D，et al.STRING v11：protein-protein association networks with increased coverage，supporting functional discovery in genome-wide experimental datasets ［J］. Nucleic Acids Res， 2019， 47（D1）： D607-D613.

HAIDER S，BALLESTER B，SMEDLEY D，et al.BioMart Central Portal-unified access to biological data ［J］. Nucleic Acids Res，2009， 37（Web Server issue）： W23-W27.

MI H， MURUGANUJAN A， HUANG X， et al.Protocol Update for large-scale genome and gene function analysis with the PANTHER classification system （v.14.0）［J］. Nat Protoc， 2019， 14（3）： 703-721.

WANG R，MEI Y，XU L，et al.Genome-wide characterization of differentially expressed genes provides insights into regulatory network of heat stress response in radish （Raphanus sativus L.）［J］. Funct Integr Genomics， 2018， 18（2）： 225-239.

METCALF S， DOUGHERTY S，KRUER T，et al.Selective loss of phosphoserine aminotransferase 1 （PSAT1） suppresses migration， invasion， and experimental metastasis in triple negative breast cancer ［J］. Clin Exp Metastasis， 2020， 37（1）： 187-197.

刘静.陆德铭教授运用扶正祛邪法治疗乳腺癌经验［J］.中医学报，2016， 31（4）： 470-473.

周维维，彭海燕.略论因“郁”致乳腺癌［J］.辽宁中医药大学学报，2016， 18（9）： 146-149.

黎明，郭秀萍，赵文静，等.中药癌复康对乳腺癌术后化疗患者生存质量的影响［J］.系统医学，2017， 2（6）： 116-119.

Views

193

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Exploration on mechanism of Baitouweng Decoction against colorectal adenoma based on network pharmacology and experimental validation

Exploration on molecular mechanism of Fructus Psoraleae in treating lung adenocarcinoma based on network pharmacology and experimental pharmacology

Exploration on mechanism of Wuling Powder combined with Taohong Siwu Decoction in treatment of heart failure based on network pharmacology， molecular docking， and experimental validation

Research progress of pharmacological actions of jujuboside A

Literature mining and bioinformatics analysis of Xue Xue’s Medical Cases for differentiation and treatment of gastrointestinal diseases

Related Author

No data

Related Institution

Institute of Digestive Diseases， Longhua Hospital， Shanghai University of Traditional Chinese Medicine

Shanghai Pudong New Area Hospital of Traditional Chinese Medicine

Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Shanghai Municipal Hospital of Traditional Chinese Medicine， Shanghai University of Traditional Chinese Medicine

Institute of Interdisciplinary Integrative Medicine Research， Shanghai University of Traditional Chinese Medicine

⁰