Effects of different concentrations of astragaloside Ⅳ on expression of eNOS， NO， ET⁃1 and PGI<sub>2</sub> in human umbilical vein endothelial cells induced by high glucose

Yuanyuan LI; Juan LUO; Chaomao YANG; Sheng ZHANG; Shunxiao ZHANG; Yan ZHANG; Yue CHEN

doi:10.16306/j.1008-861x.2022.S1.042

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Effects of different concentrations of astragaloside Ⅳ on expression of eNOS， NO， ET⁃1 and PGI₂ in human umbilical vein endothelial cells induced by high glucose

Experiment Research | 更新时间：2022-08-29

- Effects of different concentrations of astragaloside Ⅳ on expression of eNOS， NO， ET⁃1 and PGI₂ in human umbilical vein endothelial cells induced by high glucose
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue S1, Pages: 192-195(2022)
- 作者机构：
  
  1.上海中医药大学附属曙光医院宝山分院内分泌科（上海　201999）
  2.上海中医药大学附属曙光医院宝山分院老年病科（上海　201999）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.S1.042
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李园园,罗娟,杨超茅等.不同浓度黄芪甲苷对高糖诱导的人脐静脉内皮细胞eNOS、NO、ET⁃1及PGI₂表达的影响[J].上海中医药大学学报,2022,36(S1):192-195.

LI Yuanyuan,LUO Juan,YANG Chaomao,et al.Effects of different concentrations of astragaloside Ⅳ on expression of eNOS， NO， ET⁃1 and PGI₂ in human umbilical vein endothelial cells induced by high glucose[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):192-195.
李园园,罗娟,杨超茅等.不同浓度黄芪甲苷对高糖诱导的人脐静脉内皮细胞eNOS、NO、ET⁃1及PGI₂表达的影响[J].上海中医药大学学报,2022,36(S1):192-195. DOI： 10.16306/j.1008-861x.2022.S1.042.

LI Yuanyuan,LUO Juan,YANG Chaomao,et al.Effects of different concentrations of astragaloside Ⅳ on expression of eNOS， NO， ET⁃1 and PGI₂ in human umbilical vein endothelial cells induced by high glucose[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):192-195. DOI： 10.16306/j.1008-861x.2022.S1.042.

摘要

目的,2,探讨不同浓度黄芪甲苷（AS-Ⅳ）对高糖处理人脐静脉内皮细胞（HUVECs）后不同时间点内皮型一氧化氮合酶（eNOS）、一氧化氮（NO）、内皮素1（ET-1）、前列环素（PGI,2,）表达的影响。,方法,2,体外培养人脐静脉内皮细胞（HUVECs），分为对照组、高糖组、10 μmol AS-Ⅳ组、20 μmol AS-Ⅳ组、50 μmol AS-Ⅳ组、100 μmol AS-Ⅳ组。采用实时聚合酶链式反应（RT-PCR）检测各组AS-Ⅳ处理后12 h、24 h、48 h ,eNOS, mRNA的表达；采用ELISA法测定12 h、24 h、48 h时细胞培养上清液中ET-1、PGI,2,浓度的变化；采用硝酸还原酶法检测培养液中的NO分泌量。,结果,2,与对照组比较，高糖组,eNOS, mRNA表达量显著降低、PGI,2,及NO的分泌量显著降低，而ET-1的分泌显著增高（,P,<,0.01）。与高糖组比较，不同浓度的AS-Ⅳ均可不同程度的增加eNOS的mRNA表达、促进PGI,2,及NO的分泌、并降低ET-1的分泌（,P,<,0.05，,P,<,0.01）。,结论,2,AS-Ⅳ可通过提高eNOS的表达，促进NO、PGI,2,分泌，抑制ET-1分泌，保护内皮细胞免于高糖损伤，改善内皮细胞功能。

Abstract

Objective： To investigate the effects of different concentrations of astragaloside IV （AS-Ⅳ） on the expression of endothelial nitric oxide synthase （eNOS）， nitric oxide （NO）， endothelin-1 （ET-1） and prostacyclin （PGI,2,） at different time points in human umbilical vein endothelial cells （HUVECs） treated with high glucose.,Methods,2,Human umbilical vein endothelial cells （HUVECs） were cultured ,in vitro ,and divided into control group， high glucose group （30 mmol/L）， 10 μmol AS-Ⅳ group， 20 μmol AS-Ⅳ group， 50 μmol AS-Ⅳ group and 100 μmol AS-Ⅳ group. Real time polymerase chain reaction （RT-PCR） was used to detect the expressions of ,eNOS, mRNA at 12 h， 24 h and 48 h after AS-Ⅳ treatment in each group. ELISA was used to determine the concentrations of ET-1 and PGI,2, in cell culture supernatant at 12 h， 24 h and 48 h， and nitrate reductase assay was used to detect the secretion of NO in the culture medium.,Results,2,Compared with the control group， the mRNA expression of eNOS， the secretion of PGI,2 ,and NO decreased significantly， while the secretion of ET-1 increased significantly in the high glucose group（,P,<,0.01）. Compared with the high glucose group， different concentrations of AS-Ⅳ increased the mRNA expression of eNOS， promoted the secretion of PGI,2, and NO， and reduce the secretion of ET-1 to varying degrees（,P,<,0.05，,P,<,0.01）.,Conclusion,2,AS-Ⅳ can prevent endothelial cells from high glucose injury and improve endothelial cell function by increasing the expression of eNOS， promoting the secretion of NO and PGI,2, and inhibiting the secretion of ET-1.

关键词

黄芪甲苷人脐静脉内皮细胞血管活性物质

Keywords

Astragaloside Ⅳhuman umbilical vein endothelial cellsvasoactive substances

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Effects of different concentrations of astragaloside Ⅳ on expression of eNOS， NO， ET⁃1 and PGI2 in human umbilical vein endothelial cells induced by high glucose

DOI：10.16306/j.1008-861x.2022.S1.042

摘要

Abstract

关键词

Keywords

references

Effects of different concentrations of astragaloside Ⅳ on expression of eNOS， NO， ET⁃1 and PGI₂ in human umbilical vein endothelial cells induced by high glucose