Characteristics of changes of intrahepatic lymphocyte subsets in mouse with hepatic fibrosis induced by carbon tetrachloride<sub> </sub>and dimethylnitrosamine and the regulatory effect of Fuzheng Huayu Formula

Dabing PING; Xin SUN; Jingshu QI; Yanyan TAO; Yuan PENG; Chenghai LIU

doi:10.16306/j.1008-861x.2022.S1.037

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Characteristics of changes of intrahepatic lymphocyte subsets in mouse with hepatic fibrosis induced by carbon tetrachlorideand dimethylnitrosamine and the regulatory effect of Fuzheng Huayu Formula

Experiment Research | 更新时间：2022-08-29

- Characteristics of changes of intrahepatic lymphocyte subsets in mouse with hepatic fibrosis induced by carbon tetrachlorideand dimethylnitrosamine and the regulatory effect of Fuzheng Huayu Formula
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue S1, Pages: 161-166(2022)
- 作者机构：
  
  1.上海中医药大学附属曙光医院肝病研究所（上海　201203）
  2.上海市中医临床重点实验室（上海　201203）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.S1.037
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平大冰,孙鑫,齐婧姝等.四氯化碳及二甲基亚硝胺诱导肝纤维化小鼠肝脏淋巴细胞亚群变化特点及扶正化瘀方调节作用[J].上海中医药大学学报,2022,36(S1):161-166.

PING Dabing,SUN Xin,QI Jingshu,et al.Characteristics of changes of intrahepatic lymphocyte subsets in mouse with hepatic fibrosis induced by carbon tetrachlorideand dimethylnitrosamine and the regulatory effect of Fuzheng Huayu Formula[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):161-166.
平大冰,孙鑫,齐婧姝等.四氯化碳及二甲基亚硝胺诱导肝纤维化小鼠肝脏淋巴细胞亚群变化特点及扶正化瘀方调节作用[J].上海中医药大学学报,2022,36(S1):161-166. DOI： 10.16306/j.1008-861x.2022.S1.037.

PING Dabing,SUN Xin,QI Jingshu,et al.Characteristics of changes of intrahepatic lymphocyte subsets in mouse with hepatic fibrosis induced by carbon tetrachlorideand dimethylnitrosamine and the regulatory effect of Fuzheng Huayu Formula[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(S1):161-166. DOI： 10.16306/j.1008-861x.2022.S1.037.

摘要

目的,2,观察四氯化碳（CCl,4,）及二甲基亚硝胺（DMN）诱导小鼠肝纤维化模型肝脏淋巴细胞亚群变化特点，并进一步观察中药扶正化瘀方对淋巴细胞亚群变化的调节作用。,方法,2,分别采用10%CCl,4,及0.5%DMN腹腔注射制备小鼠肝纤维化模型，观察小鼠肝脏大体观及脏器指数，检测小鼠血清ALT、AST表达变化，HE和天狼猩红染色观察肝组织炎症及纤维化程度，流式细胞术观察小鼠肝脏淋巴细胞亚群比例和数量变化。,结果,2,与正常组比较，两种模型组小鼠肝脏表面粗糙，质地变硬，粟粒样表现明显，肝体比下降，脾体比升高，血清ALT、AST表达增加，肝组织病理染色显示明显的炎症和胶原沉积，肝脏NK细胞、NKT细胞、CD4,+,T细胞比例和数量下降，CD8,+,T细胞和B细胞比例和数量升高（,P,<,0.05，,P,<,0.01）；与模型组比较，药物组小鼠肝脏大体观明显改善，肝体比增加，脾体比下降，血清ALT、AST表达下降，肝组织炎症和胶原沉积明显减轻，肝脏NK细胞、NKT细胞、CD4,+,T细胞比例和数量升高，CD8,+,T细胞和B细胞比例和数量下降（,P,<,0.05，,P,<,0.01）。,结论,2,CCl,4,和DMN诱导肝纤维化模型小鼠肝脏淋巴细胞亚群免疫紊乱，中药扶正化瘀方可调节肝脏免疫微环境，纠正免疫紊态，发挥抗肝纤维化作用。

Abstract

关键词

扶正化瘀方肝纤维化CCl4DMN淋巴细胞

Keywords

Fuzheng Huayu Formulahepatic fibrosisCCl4DMNlymphocyte

references

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Shanghai University of Traditional Chinese Medicine， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Institute of Hepatology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Hepatology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Department of Hepatology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

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Characteristics of changes of intrahepatic lymphocyte subsets in mouse with hepatic fibrosis induced by carbon tetrachloride and dimethylnitrosamine and the regulatory effect of Fuzheng Huayu Formula

DOI：10.16306/j.1008-861x.2022.S1.037

摘要

Abstract

关键词

Keywords

references

Characteristics of changes of intrahepatic lymphocyte subsets in mouse with hepatic fibrosis induced by carbon tetrachlorideand dimethylnitrosamine and the regulatory effect of Fuzheng Huayu Formula