Objective： To explore the pathological mechanism of the imbalance of “liver-bile acid-intestinal microbiological axis” in non-alcoholic fatty liver disease （NAFLD） model mice induced by high-fat diet， verify the regulation of Cangju Qinggan Formula on the imbalance of “liver-bile acid-intestinal microbiological axis”， and further illustrate the mechanism of Cangju Formula in the treatment of NAFLD.,Methods,2,Thirty-two SPF male SD rats were randomly divided into blank group （N， ,n,=5） and high-fat diet group （HFD， ,n,=27）. Five mice in blank group were only given ordinary diet， and high-fat diet group was fed with hrgh-fat diet （cholestenol 1%，lard 10%， sucrose 20%） to induce NAFLD model. After 8 weeks of feeding， 25 rats in the high-fat diet group were randomly divided into model group （HFDM， ,n,=5）， probiotic group （YSJ， ,n,=5）， Cangju Formula high-dose group （CJFH， ,n,=5）， Cangju Formula medium-dose group （CJFM， ,n,=5）， Cangju Formula low-dose group （CJFL， ,n,=5）. The contents of serum alanine aminotransferase （ALT） ， aspartate aminotransferase （AST） ， high density lipoprotein cholesterol （HDL-C）， low density lipoprotein cholesterol （LDL-C）， triglyceride （TG） and total cholesterol （TC） in serum of rats in each group were measured by automatic biochemical instrument. The degree of liver lesion and lipid deposition in rats were observed by HE staining. Using Illumina high-throughput sequencing method， the V3~V4 region of 16sr RNA of total DNA of rat fecal samples was sequenced and bioinformatics was analyzed.,Results,2,Compared with blank group， serum ALT and AST increased， TC， TG and LDL-C increased， and HLD-C decreased in model group， the differences were statistically significant （,P,<,0.05）. Compared with the model group， serum ALT， AST and TC decreased in the probiotic group and Cangju Formula groups （,P,<,0.05）， serum TG decreased in Cangju Formula medium and high dose group （,P,<,0.05）， serum HDD-C increased in the probiotic group， Cangju Formula medium and high dose group， and serum LDL-C decreased in Cangju Formula high-dose group （,P,<,0.05）. HE staining showed that hepatocytes in the model group presented diffuse vacuoles of different sizes and balloon-like changes， the infiltration of inflammatory cells in the portal area was obvious， and the hepatocytes were disordered with lots of lipid droplet of different sizes and dark color. All treatment groups could improve the damage degree of liver cells in NAFLD rats， with partially scattered vacuoles， and some liver cells returned to normal. The intestinal microflora of Cangju Formula groups were different from those of model group， and gradually approached to the state of the normal group.,Conclusion,2,Cangju Formula can reduce the levels of ALT， AST， TC， TG， LDL-C in serum of rats， and increase the level of HDL-C in serum of rats. It can also improve the pathological status and lipid deposition of NAFLD rats. And it improves NAFLD mainly by improving the number of community species and gradually restoring the composition and structure of disturbed intestinal flora in NAFLD rats fed with high-fat diet to normal.