Mechanism study of modified Wuzi Yanzong Pill on prevention and treatment of osteoporosis in ApoE<sup>-/-</sup> mice based on retinoid X receptor expression

WANG Jie; RUAN Ming; ZHUANG Chengyu; BIAN Dongsheng; HUANG Jingjing; YANG Renhao; WANG Lei; YU Pei

doi:10.16306/j.1008-861x.2022.06.011

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Mechanism study of modified Wuzi Yanzong Pill on prevention and treatment of osteoporosis in ApoE^-/- mice based on retinoid X receptor expression

Experiment Research | 更新时间：2022-11-26

- Mechanism study of modified Wuzi Yanzong Pill on prevention and treatment of osteoporosis in ApoE^-/- mice based on retinoid X receptor expression
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue 6, Pages: 70-75(2022)
- 作者机构：
  
  1.上海交通大学医学院附属瑞金医院中医科（上海　200025）
  2.上海交通大学医学院附属瑞金医院骨科（上海　200025）
  3.上海交通大学医学院附属瑞金医院临床营养科（上海　200025）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.06.011
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王洁,阮铭,庄澄宇等.加味五子衍宗丸基于视黄素X受体表达防治ApoE^-/-小鼠骨质疏松的作用机制研究[J].上海中医药大学学报,2022,36(06):70-75.

WANG Jie,RUAN Ming,ZHUANG Chengyu,et al.Mechanism study of modified Wuzi Yanzong Pill on prevention and treatment of osteoporosis in ApoE^-/- mice based on retinoid X receptor expression[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(06):70-75.
王洁,阮铭,庄澄宇等.加味五子衍宗丸基于视黄素X受体表达防治ApoE^-/-小鼠骨质疏松的作用机制研究[J].上海中医药大学学报,2022,36(06):70-75. DOI： 10.16306/j.1008-861x.2022.06.011.

WANG Jie,RUAN Ming,ZHUANG Chengyu,et al.Mechanism study of modified Wuzi Yanzong Pill on prevention and treatment of osteoporosis in ApoE^-/- mice based on retinoid X receptor expression[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(06):70-75. DOI： 10.16306/j.1008-861x.2022.06.011.

摘要

目的,2,探讨加味五子衍宗丸防治载脂蛋白E敲除（ApoE,-/-,）小鼠骨质疏松的相关作用机制。,方法,2,8周龄ApoE,-/-,小鼠随机分为阴性对照组、模型组、治疗组和阳性对照组，每组6只。阴性对照组和模型组小鼠给予常规饲料；治疗组和阳性对照组小鼠给予高脂饲料，同时分别灌胃给予加味五子衍宗丸和阿仑膦酸钠悬液。各组小鼠连续干预24周后，取股骨做微型电子计算机断层扫描（Micro CT）、HE染色、Trap染色及免疫组化染色，观察各组小鼠骨质疏松情况、破骨细胞数量及视黄素X受体（RXR）的表达情况。,结果,2,①与阴性对照组比较，模型组小鼠股骨Micro CT检测指标骨密度（BMD）、骨体积分数（BV/TV）、骨小梁厚度（Tb.Th）和骨小梁数量（Tb.N）均显著下降（,P,<,0.05）；与模型组相比，治疗组的BMD、BV/TV、Tb.Th、Tb.N均显著增加（,P,<,0.05）。②与阴性对照组比较，模型组骨质疏松面积显著增加（,P,<,0.01）；与模型组比较，治疗组及阳性对照组骨质疏松面积显著减少（,P,<,0.01）。③模型组破骨细胞数量显著高于阴性对照组（,P,<,0.01），治疗组和阳性对照组破骨细胞数量则显著低于模型组（,P,<,0.01）。④与阴性对照组比较，模型组小鼠RXRα和RXRβ蛋白表达显著增加（,P,<,0.01）；与模型组比较，治疗组和阳性对照组小鼠RXRα和RXRβ蛋白表达显著减少（,P,<,0.01）。,结论,2,加味五子衍宗丸可抑制ApoE,-/-,小鼠股骨骨质疏松的进展，其作用机制可能与抑制RXR的表达及破骨细胞增殖有关。

Abstract

Objective： To investigate the related mechanism of modified Wuzi Yanzong Pill on prevention and treatment of osteoporosis in apolipoprotein E knockout（ApoE,-/-,） mice.,Methods,2,The 8-week-old ApoE,-/-, mice were randomly divided into negative control group， model group， treatment group and positive control group， with 6 mice in each group. The mice in negative control group and model group were fed with normal diet. The mice in treatment group and positive control group were fed with high fat diet， supplemented with modified Wuzi Yanzong Pill and alendronate sodium by gavage respectively. After 24 weeks of continuous intervention， femurs of mice in each group were taken for micro computed tomography （Micro CT）， HE staining， Trap staining and immumohistochemical staining to observe the status of osteoporosis， the number of osteoclasts and the expression of retinoid X receptor （RXR） in each group.,Results,2,①Compared with the negative control group， femur Micro CT detection indexes of bone mineral density （BMD）， bone volume fraction （BV/TV）， trabecular thickness （Tb.Th） and trabecular number （Tb.N） in model group were significantly decreased （,P,<,0.05）. Compared with the model group， BMD， BV/TV， Tb.Th and Tb.N in treatment group were significantly increased （,P,<,0.05）. ②Compared with the negative control group， the osteoporosis area in the model group was significantly increased （,P,<,0.01）. Compare with the model group， the area of osteoporosis in the treatment group and the positive control group was significantly decreased （,P,<,0.01）. ③The numbers of osteoclasts in the model group were significantly higher than that in the negative control group （,P,<,0.01）， while the number of osteoclasts in the treatment group and positive control group was significantly lower than that in the model group（,P,<,0.01）. ④Compared with the negative control group， the expression of RXRα and RXRβ protein in the model group was significantly increased （,P,<,0.01）； Compared with the model group， the expression of RXRα and RXRβ protein in the treatment group and positive control group was significantly decreased （,P,<,0.01）.,Conclusion,2,Modified Wuzi Yanzong Pill could inhibit the progression of femur osteoporosis in ApoE,-/-,mice， and the mechanism may be related to the inhibition of RXR expression and osteoclast proliferation.

关键词

加味五子衍宗丸骨质疏松ApoE-/-小鼠高脂饮食视黄素X受体

Keywords

modified Wuzi Yanzong PillosteoporosisApoE-/- micehigh fat dietretinoid X receptor

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Mechanism study of modified Wuzi Yanzong Pill on prevention and treatment of osteoporosis in ApoE-/- mice based on retinoid X receptor expression

DOI：10.16306/j.1008-861x.2022.06.011

摘要

Abstract

关键词

Keywords

references

Mechanism study of modified Wuzi Yanzong Pill on prevention and treatment of osteoporosis in ApoE^-/- mice based on retinoid X receptor expression