Molecular pharmacognostical identification of mainstream varieties of Tibetan medicine Jieji Gabao from Sichuan and Gansu in China

ALA Kelsang Gyab; NI Lianghong; ZHAO Zhili; KANG Heng; WU Jinrong; GAAWE Dorje

doi:10.16306/j.1008-861x.2022.06.004

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Molecular pharmacognostical identification of mainstream varieties of Tibetan medicine Jieji Gabao from Sichuan and Gansu in China

Special column on Identification of traditional Chinese medicine | 更新时间：2022-11-26

- Molecular pharmacognostical identification of mainstream varieties of Tibetan medicine Jieji Gabao from Sichuan and Gansu in China
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue 6, Pages: 22-29(2022)
- 作者机构：
  
  1.上海中医药大学中药学院（上海　201203）
  2.西藏自治区藏医院（西藏　拉萨　850000）
  3.西藏藏医药大学（西藏　拉萨　　850000）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.06.004
  CLC：
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尕让甲,倪梁红,赵志礼等.川甘产藏药解吉嘎保主流品种的分子生药学鉴定[J].上海中医药大学学报,2022,36(06):22-29.

ALA Kelsang Gyab,NI Lianghong,ZHAO Zhili,et al.Molecular pharmacognostical identification of mainstream varieties of Tibetan medicine Jieji Gabao from Sichuan and Gansu in China[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(06):22-29.
尕让甲,倪梁红,赵志礼等.川甘产藏药解吉嘎保主流品种的分子生药学鉴定[J].上海中医药大学学报,2022,36(06):22-29. DOI： 10.16306/j.1008-861x.2022.06.004.

ALA Kelsang Gyab,NI Lianghong,ZHAO Zhili,et al.Molecular pharmacognostical identification of mainstream varieties of Tibetan medicine Jieji Gabao from Sichuan and Gansu in China[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(06):22-29. DOI： 10.16306/j.1008-861x.2022.06.004.

摘要

目的,2,梳理川甘产藏药“解吉嘎保”（,http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=37277894&type=,http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=37277902&type=,http://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=37277898&type=,14.15923691,4.44414043,）主流品种，并基于DNA条形码对其进行分子生药学鉴定研究。,方法,2,在川甘产区进行民族植物学考察，共采集11个居群55份样品标本，对其进行基原、药材性状、显微鉴定及质量评价；并基于课题组前期相关工作进一步筛选DNA分子标记，测定各样品及西藏产麻花艽与秦艽组Sect. ,Cruciatar ,9个近缘物种的叶绿体基因组,psbA⁃trnH,、,rpl20⁃rps12,及线粒体基因组,nad1, b/c序列，以龙胆科花锚属椭圆叶花锚,Halenia elliptica,及龙胆属,Gentiana,高山龙胆组Sect. ,Frigidat,直萼龙胆,Gentiana erecto⁃sepala,为外类群，邻接法（neighbor-joining）构建系统发育树，评价各片段的系统学意义，构建DNA条形码。,结果,2,①川甘产藏药解吉嘎保的主流品种为龙胆科龙胆属秦艽组植物麻花艽,Gentiana straminea,。②各样品中龙胆苦苷和马钱苷酸总含量符合《中华人民共和国药典（2020年版）》的标准。③叶绿体,psbA⁃trnH,序列可将秦艽组10个物种分为两类，麻花艽与粗壮秦艽,Gentiana robusta,为一类，其余8个近缘物种为另一大类；线粒体,nad1, b/c序列可进一步区分麻花艽与粗壮秦艽；叶绿体,rpl20⁃rps12,序列则具有麻花艽种下不同产地间分辨率。因此，构建川甘产藏药解吉嘎保主流品种麻花艽基于叶绿体,psbA⁃trnH,、线粒体,nad1, b/c、叶绿体,rpl20⁃rps12,三个片段的组合型DNA条形码。,结论,2,本研究明确了川甘产藏药解吉嘎保的主流品种来源，并构建了其DNA条形码鉴定方法，可为解吉嘎保的基原植物鉴定、道地藏药材质量标准的提升及龙胆科植物系统发育研究等提供基础资料。

Abstract

Objective,2,To investigate the mainstream varieties of Tibetan medicine “Jieji Gaobao” from Sichuan and Gansu in China， and study the method of molecular pharmacognosy identification based on DNA barcoding.,Methods,2,An ethnobotanical survey of “Jieji Gabao” in Sichuan and Gansu was carried out and 55 samples from 11 populations were collected. The origin， medicinal material morphological and microscopic identification as well as quality evaluation of Jieji Gaobao were carried out. Based on our previous work， the DNA molecular markers were further screened. The sequences of chloroplast genome ,psbA⁃trnH,，, rpl20⁃rps12, and mitochondrial genome, nad1, b/c in each sample and 9 related species of ,Gentiana, Sect. ,Cruciata. ,The phylogenetic tree was constructed by neighbor-joining method with ,Halenia elliptica, （Gentianaceae） and ,Gentiana erecto⁃sepala ,（,Gentiana ,Sect. ,Frigidat,） as the outgroups. Then the phylogenetic significances of each fragment were evaluated and DNA barcodes were constructed.,Results,2,①,Gentiana straminea, （Gentianaceae ,Gentiana,） from Sichuan and Gansu is the mainstream variety of Tibetan medicine Jieji Gabao. ②The total content of gentiopicroside and loganic acid in each sample meets the standard of ,Chinese Pharmacopoeia,. ③The chloroplast ,psbA⁃trnH, sequence could divide the 10 species into two groups， with ,Gentiana straminea, and ,Gentiana robusta ,in one group and the other 8 related species in another group； the mitochondrial ,nad1, b/c sequence could further distinguish ,Gentiana straminea, and ,Gentiana robusta,； the chloroplast, rpl20⁃rps12 ,sequence had the resolution between different origin. Therefore， the combinational DNA barcodes based on chloroplast ,psbA⁃trnH,， mitochondrial ,nad1, b/c， and chloroplast ,rpl20⁃rps12, were constructed for ,Gentiana straminea,， the mainstream Tibetan medicine “Jieji Gabao” from Sichuan and Gansu.,Conclusion,2,In this study， the origin of the mainstream varieties of Tibetan medicine Jieji Gabao from Sichuan and Gansu were clarified， and its identification method based on DNA barcoding was constructed. This study could provide basic data for identifying the original plants of Tibetan medicine Jieji Gabao， improving the quality standard of Tibetan genuine medicinal materials and studying the phylogeny of Gentianaceae plants.

关键词

解吉嘎保藏药麻花艽分子生药学DNA条形码

Keywords

Jieji GabaoTibetan medicineGentiana stramineamolecular pharmacognosyDNA barcoding

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