Identification and analysis of ribosomal RNA gene and its intergene （ITSs） in Ganoderma and edible fungus based on third⁃generation sequencing technology

ZHANG Weiwei; YUAN Yuhong; GE Yaqian; ZHU Yanrong; ZHAO Jienan; SUN Jianhe; HUANG Linjia; HU Zehua; XU Jiahao; GUO Liting; GAO Yufeng; ZHANG Yihui; WANG Yanping; HUANG Gang; WANG Yixing; DAI Fangping

doi:10.16306/j.1008-861x.2022.06.002

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Identification and analysis of ribosomal RNA gene and its intergene （ITSs） in Ganoderma and edible fungus based on third⁃generation sequencing technology

Special column on Identification of traditional Chinese medicine | 更新时间：2022-11-26

- Identification and analysis of ribosomal RNA gene and its intergene （ITSs） in Ganoderma and edible fungus based on third⁃generation sequencing technology
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue 6, Pages: 5-12(2022)
- 作者机构：
  
  1.江苏中方基因生物医学科技有限公司（江苏　南通　226126）
  2.南通大学附属海门医院（江苏　南通　226121）
  3.安徽中医药大学（安徽　合肥　230012）
  4.江西中医药大学（江西　南昌　330004）
  5.上海健康医学院（上海　200237）
  6.同济大学附属东方医院（上海　200120）
  7.长三角药学高等工程学院（江苏　南通　226126）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.06.002
  CLC：
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张韦唯,袁育红,戈雅倩等.基于三代测序技术对中药灵芝和食用菌rRNA基因及其基因间序列的鉴定研究[J].上海中医药大学学报,2022,36(06):5-12.

ZHANG Weiwei,YUAN Yuhong,GE Yaqian,et al.Identification and analysis of ribosomal RNA gene and its intergene （ITSs） in Ganoderma and edible fungus based on third⁃generation sequencing technology[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(06):5-12.
张韦唯,袁育红,戈雅倩等.基于三代测序技术对中药灵芝和食用菌rRNA基因及其基因间序列的鉴定研究[J].上海中医药大学学报,2022,36(06):5-12. DOI： 10.16306/j.1008-861x.2022.06.002.

ZHANG Weiwei,YUAN Yuhong,GE Yaqian,et al.Identification and analysis of ribosomal RNA gene and its intergene （ITSs） in Ganoderma and edible fungus based on third⁃generation sequencing technology[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(06):5-12. DOI： 10.16306/j.1008-861x.2022.06.002.

摘要

目的,2,基于三代PacBio测序技术，建立中药灵芝和食用菌rRNA 18S-ITS-28S全长序列文库，从分子水平快速、准确鉴定和研究品种。,方法,2,对收集到的8种食用菌和1种中药材真菌灵芝抽提基因组DNA，设计引物扩增18S-ITS-28S全长序列，18S-ITS-28S全长序列包括连续且完整的18S rRNA-ITS1-5.8S rRNA-ITS2和28S rRNA部分序列，其总长度>3.3 kb。将18S-ITS-28S全长序列进行建库和三代PacBio测序。,结果,2,食用菌和药用灵芝的18S rRNA、5.8S rRNA及部分28S rRNA基因序列相似度高且序列保守，而rRNA基因间（ITS1和ITS2）的序列差异性大。,结论,2,本研究的结果提示，分析rRNA基因间（ITSs）区域，而不是仅仅分析rRNA基因，有助于真菌类中药材的鉴定和研究。

Abstract

Objective： Based on the third-generation PacBio sequencing technology， to establish a full-length sequence database of Ganoderma and edible fungi rRNA 18S-ITS-28S， and to identify species quickly and accurately at the molecular level.,Methods,2,DNA was extracted from 8 kinds of edible fungi and 1 kind of ,Ganoderma lucidum ,which previously collected， and used primers to amplify the full-length sequence of 18S-ITS-28S. The full-length sequence of 18S-ITS-28S included continuous and complete 18S rRNA， 5.8S rRNA and partial 28S rRNA sequence， the total length is longer than 3.3 kb， the PacBio third-generation sequenceing was used to measure the full-length sequence of each sample 18S-ITS-28S samples and construct library.,Results,2,The 18S rRNA， 5.8S rRNA， and partial 28S rRNA genes of edible fungi and medicinal ,Ganoderma lucidum, were highly similar and conserved. The rRNA genes （ITS1 and ITS2） were significantly different.,Conclusion,2,The results of this study suggest that the analysis of rRNA intergenic （ITSs） regions， instead of only the analysis of rRNA genes， is helpful for the identification and research of fungal Chinese medicinal materials.

关键词

灵芝食用菌rRNA基因测序分析鉴定

Keywords

Ganodermaedible fungusrRNA genesequencing analysisidentification

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