Exploring mechanism of Mori Folium⁃Carthami Flos on type 2 diabetes based on network pharmacology

ZHOU Yi; NA Lixin

doi:10.16306/j.1008-861x.2022.05.013

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Exploring mechanism of Mori Folium⁃Carthami Flos on type 2 diabetes based on network pharmacology

Experiment Research | 更新时间：2022-11-01

- Exploring mechanism of Mori Folium⁃Carthami Flos on type 2 diabetes based on network pharmacology
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue 5, Pages: 77-85(2022)
- 作者机构：
  
  1.上海中医药大学研究生院（上海　201203）
  2.上海健康医学院健康与公共卫生学院（上海　201318）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.05.013
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周熠,那立欣.基于网络药理学的桑叶-红花治疗2型糖尿病作用机制探讨[J].上海中医药大学学报,2022,36(05):77-85.

ZHOU Yi,NA Lixin.Exploring mechanism of Mori Folium⁃Carthami Flos on type 2 diabetes based on network pharmacology[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(05):77-85.
周熠,那立欣.基于网络药理学的桑叶-红花治疗2型糖尿病作用机制探讨[J].上海中医药大学学报,2022,36(05):77-85. DOI： 10.16306/j.1008-861x.2022.05.013.

ZHOU Yi,NA Lixin.Exploring mechanism of Mori Folium⁃Carthami Flos on type 2 diabetes based on network pharmacology[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(05):77-85. DOI： 10.16306/j.1008-861x.2022.05.013.

摘要

目的,2,基于网络药理学探讨桑叶-红花治疗2型糖尿病的作用机制。,方法,2,基于中药系统药理数据库和分析平台（TCMSP）、治疗靶标数据库（TTD）等进行药物与疾病靶点收集，利用Cytoscape软件构建“有效成分-关键靶点-通路”网络图；基于DAVID 6.8数据库进行GO富集分析与KEGG分析，找出关键靶点及关键成分，并利用AutoDock Vina软件进行分子对接验证；进行细胞实验，验证关键化学成分干预HepG2胰岛素抵抗模型细胞的效果。,结果,2,①网络拓扑分析结果表明，关键药物成分可能为黄芩苷、红厚壳内酯等，关键靶点可能为肿瘤坏死因子（TNF）、AKT丝氨酸/苏氨酸蛋白激酶1（AKT1）等。②KEGG分析发现桑叶-红花抗2型糖尿病主要与胰岛素信号传导通路、腺苷酸活化蛋白激酶（AMPK）通路等信号通路有关。③分子对接发现AKT1与黄芩苷、TNF与红厚壳内酯对接效果最好。④细胞实验证明，20 μg/ml黄芩苷及10 μg/ml红厚壳内酯对HepG2细胞无毒性，且干预HepG2模型细胞后，可改善细胞的胰岛素敏感性。,结论,2,桑叶-红花可能通过胰岛素信号传导通路、胰岛素抵抗相关通路等通路的协同作用发挥治疗2型糖尿病的作用，其关键药效成分可能为黄芩苷和红厚壳内酯。

Abstract

Objective： To explore the mechanism of Mori Folium-Carthami Flos on type 2 diabetes based on network pharmacology.,Methods,2,The targets of drugs and disease were collected based on Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform （TCMSP） and Therapeutic Target Database （TTD）， and the network diagram of “active component-key target-pathway” was constructed by Cytoscape software. GO enrichment analysis and KEGG analysis were carried out based on DAVID 6.8 database to identify key targets and key components， and AutoDock Vina Software was used for molecular docking verification. Cell experiments were performed to verify the effects of key chemical components on insulin resistant model cells.,Results,2,①The results of network topology analysis showed that the key drug components might be baicalin and inophyllum E， and the key targets might be tumor necrosis factor （TNF） and AKT serine/threonine protein kinase 1（AKT1）. ②KEGG analysis showed that the anti-type 2 diabetes of Mori Folium-Carthami Flos was mainly associated with signal pathways such as insulin signaling pathway and adenosine-activated protein kinase （AMPK） pathway. ③Molecular docking showed that AKT1 and baicalin， TNF and inophyllum E had the best molecular docking effect. ④The cell experiments demonstrated that 20 μg/ml baicalin and 10 μg/ml inophyllum E were not toxic to HepG2 cells and could improve the insulin sensitivity of the cells in HepG2 model cells after intervention.,Conclusion,2,Mori Folium-Carthami Flos may play a synergistic role in the treatment of type 2 diabetes through pathways such as insulin signal transduction pathway and insulin resistance related pathway， and the key pharmacodynamic components may be baicalin and inophyllum E.

关键词

桑叶-红花2型糖尿病黄芩苷AKT1网络药理学

Keywords

Mori Folium-Carthami Flostype 2 diabetesbaicalinAKT1network pharmacology

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