Study on <italic style="font-style: italic">in vitro</italic> release of berberine hydrochloride thermosensitive hydrogel based on nano graphene oxide

Weiwei ZHI; Huahua WANG; Shasha WANG; Ning ZHOU; Yongyan JIA; Xiali ZHU

doi:10.16306/j.1008-861x.2022.03.011

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Study on in vitro release of berberine hydrochloride thermosensitive hydrogel based on nano graphene oxide

Experiment Research | 更新时间：2022-08-29

- Study on in vitro release of berberine hydrochloride thermosensitive hydrogel based on nano graphene oxide
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue 3, Pages: 66-71(2022)
- 作者机构：
  
  1.河南中医药大学药学院（河南　郑州　450046）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.03.011
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直炜炜,王华华,王莎莎等.基于纳米氧化石墨烯的盐酸小檗碱温敏水凝胶体外释放度研究[J].上海中医药大学学报,2022,36(03):66-71.

ZHI Weiwei,WANG Huahua,WANG Shasha,et al.Study on in vitro release of berberine hydrochloride thermosensitive hydrogel based on nano graphene oxide[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(03):66-71.
直炜炜,王华华,王莎莎等.基于纳米氧化石墨烯的盐酸小檗碱温敏水凝胶体外释放度研究[J].上海中医药大学学报,2022,36(03):66-71. DOI： 10.16306/j.1008-861x.2022.03.011.

ZHI Weiwei,WANG Huahua,WANG Shasha,et al.Study on in vitro release of berberine hydrochloride thermosensitive hydrogel based on nano graphene oxide[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(03):66-71. DOI： 10.16306/j.1008-861x.2022.03.011.

摘要

目的,2,建立基于纳米氧化石墨烯的盐酸小檗碱温敏水凝胶（BH-NGO-gel）中盐酸小檗碱及其体外释放度的测定方法。,方法,2,采用高效液相色谱法，C,18,色谱柱（4.6 mm×250 mm， 5 μm）；流动相乙腈-0.03 mol/L磷酸二氢钾（28∶72， v/v）；检测波长350 nm；流速1 ml/min；柱温30 ℃；进样量10 μl。释放介质为含0.5%吐温80的0.1 mol/L磷酸盐缓冲液（PBS， pH 7.4），采用透析法分别于37 ℃和42 ℃条件下进行体外释放度试验，并采用DDSolver软件拟合药物释放模型。,结果,2,盐酸小檗碱的线性范围为2～20 μg/ml，高、中、低3个浓度的回收率均,>,95%，,RSD,均,<,2%。相比于盐酸小檗碱溶液，BH-NGO-gel释药速度明显较慢（,P,<,0.001）；42 ℃释药速率高于37 ℃，但无明显统计学差异（,P,>,0.05）。BH-NGO-gel在37 ℃和42 ℃ 下的释药过程均符合Makoid-Banakar释药模型，相关系数,r,分别为0.993 6和0.986 9。,结论,2,建立的BH-NGO-gel中盐酸小檗碱体外释放度测定方法简便、可行。BH-NGO-gel具有明显的缓释作用，且具有温度依赖性的释药特征。

Abstract

Objective： To establish a determination method for berberine hydrochloride and its ,in vitro, release of berberine hydrochloride thermosensitive hydrogel based on nano graphene oxide （BH-NGO-gel）.,Methods,2,The high performance liquid chromatography method was used， performed on a C,18, column （4.6 mm × 250 mm， 5 μm） at a flow rate of 1 ml/min with mobile phase of acetonitrile-0.03 mol/L monobasic potassium phosphate （28∶72， v/v）. The detection wavelength was 350 nm， the column temperature was 30 ℃ and the injection volume was 10 μl. The release medium was 0.1 mol/L phosphate buffer （PBS， pH 7.4） containing 0.5% Tween 80. The ,in vitro ,release tests were carried out by dialysis method at 37 ℃ and 42 ℃ respectively. And the kinetic model of drug release was analyzed by DDSolver software.,Results,2,The linear range of berberine hydrochloride was 2~20 μg/ml， and the recoveries of high， medium and low concentrations were all more than 95%， and, RSD, were all less than 2%. Compared with berberine hydrochloride solution， the drug release rate of BH-NGO-gel was significantly slower （,P,<,0.001）， and the drug release rate at 42 ℃ was higher than that of 37 ℃， but there was no significant statistical difference （,P,>,0.05）. The ,in vitro, release processes of BH-NGO-gel at 42 ℃ and 37 ℃ were all accorded with Makoid-Banakar model， and the correlation coefficients ,r, was 0.993 6 and 0.986 9， respectively.,Conclusion,2,The established method for determining berberine hydrochloride ,in vitro, release from BH-NGO-gel is simple and feasible. BH-NGO-gel had obvious sustained release effect and temperature dependent drug release characteristics.

关键词

盐酸小檗碱温敏水凝胶纳米氧化石墨烯体外释放度高效液相色谱法

Keywords

berberine hydrochloridethermosensitive hydrogelnano graphene oxidein vitro releasehigh performance liquid chromatography

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Study on in vitro release of berberine hydrochloride thermosensitive hydrogel based on nano graphene oxide

DOI：10.16306/j.1008-861x.2022.03.011

摘要

Abstract

关键词

Keywords

references