Study on content determination and fingerprint of main components of total phenol in Phyllanthi Fructus

Tao YU; Chuncao ZHAO; Jiquan ZHANG

doi:10.16306/j.1008-861x.2022.03.010

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Study on content determination and fingerprint of main components of total phenol in Phyllanthi Fructus

Experiment Research | 更新时间：2022-08-29

- Study on content determination and fingerprint of main components of total phenol in Phyllanthi Fructus
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue 3, Pages: 61-65(2022)
- 作者机构：
  
  1.上海方心制药科技有限公司（上海　201611）
  2.上海市生物医药技术研究院（上海　200032）
  3.上海中医药大学中药现代制剂技术工程研究中心（上海　201203）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.03.010
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于涛,赵春草,张继全.余甘子总酚主要成分的含量测定和指纹图谱研究[J].上海中医药大学学报,2022,36(03):61-65.

YU Tao,ZHAO Chuncao,ZHANG Jiquan.Study on content determination and fingerprint of main components of total phenol in Phyllanthi Fructus[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(03):61-65.
于涛,赵春草,张继全.余甘子总酚主要成分的含量测定和指纹图谱研究[J].上海中医药大学学报,2022,36(03):61-65. DOI： 10.16306/j.1008-861x.2022.03.010.

YU Tao,ZHAO Chuncao,ZHANG Jiquan.Study on content determination and fingerprint of main components of total phenol in Phyllanthi Fructus[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(03):61-65. DOI： 10.16306/j.1008-861x.2022.03.010.

摘要

目的,2,建立同时测定余甘子总酚中没食子酸和葡糖倍苷的UPLC含量测定方法，并进行余甘子总酚的UPLC指纹图谱研究。,方法,2,采用CAPCELL PAK C,18, MG（4.6 mm × 150 mm，5 μm）色谱柱；流动相：甲醇-2%乙酸水溶液，梯度洗脱；检测波长：273 nm；柱温：30 ℃；流速：0.8 ml/min。,结果,2,没食子酸和葡糖倍苷分别在5.82~465.20 μg/ml、12.38~793.00 μg/ml范围内呈良好线性关系；平均加样回收率分别为100.57%和99.22%，,RSD,均,<,3.0%；指纹图谱共标示出7个特征峰，相似度,>,0.90。,结论,2,所建立的方法简便、结果准确、重复性好，可作为余甘子总酚原料的质量控制方法。

Abstract

objective： ,To establish a UPLC assay method for the determination of gallic acid and glucose times nucleoside and a UPLC fingerprint analysis method for total phenol of Phyllanthi Fructus.,Methods,2,The analysis conditions were set at a CAPCELL PAK C,18, MG（4.6 mm × 150 mm，5 μm）chromatographic column； mobile phase： methanol-2% acetic acid aqueous solution， gradient elution； detection wavelength： 273 nm； column temperature： 30 ℃； flow rate： 0.8 ml/min.,Results,2,Good linear relationship was observed for gallic acid in the range of 5.82~465.20 μg/ml and glucose times nucleoside in the range of 12.38~793.00 μg/ml respectively. And the average recovery rates of gallic acid and glucoside were 100.57% and 99.22% respectively， with the ,RSD,s both less than 3.0%. The fingerprint showed 7 characteristic peaks， and the similarity of fingerprint was more than 0.90.,Conclusion,2,This method is simple， accurate and reproducible， and could be used as a quality control method for total phenol of Phyllanthi Fructus.

关键词

余甘子总酚没食子酸葡糖倍苷含量测定指纹图谱

Keywords

total phenol of Phyllanthi Fructusgallic acidglucose times nucleosidecontent determinationfingerprint

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