Establishment of combination of disease with syndrome mouse model with Parkinson’s disease and study on the attributes of TCM syndrome

Pengfei XUN; Zhuqing HE; Li WANG; Yingdi WANG; Jiancheng HE

doi:10.16306/j.1008-861x.2022.02.007

您当前的位置：

首页 >

文章列表页 >

Establishment of combination of disease with syndrome mouse model with Parkinson’s disease and study on the attributes of TCM syndrome

Experiment Research | 更新时间：2022-08-29

- Establishment of combination of disease with syndrome mouse model with Parkinson’s disease and study on the attributes of TCM syndrome
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 36, Issue 2, Pages: 36-42(2022)
- 作者机构：
  
  1.上海中医药大学基础医学院，上海市健康辨识与评估重点实验室（上海　201203）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2022.02.007
  CLC：
扫描看全文
郇鹏飞,何竹青,王利等.病证结合帕金森病小鼠模型的构建及中医证候属性研究[J].上海中医药大学学报,2022,36(02):36-42.

XUN Pengfei,HE Zhuqing,WANG Li,et al.Establishment of combination of disease with syndrome mouse model with Parkinson’s disease and study on the attributes of TCM syndrome[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(02):36-42.
郇鹏飞,何竹青,王利等.病证结合帕金森病小鼠模型的构建及中医证候属性研究[J].上海中医药大学学报,2022,36(02):36-42. DOI： 10.16306/j.1008-861x.2022.02.007.

XUN Pengfei,HE Zhuqing,WANG Li,et al.Establishment of combination of disease with syndrome mouse model with Parkinson’s disease and study on the attributes of TCM syndrome[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2022,36(02):36-42. DOI： 10.16306/j.1008-861x.2022.02.007.

摘要

目的,2,建立病证结合帕金森病（PD）小鼠模型，并探讨其中医证候属性。,方法,2,采用1-甲基-4-苯基-1，2，3，6-四氢吡啶（MPTP）慢性腹腔注射的方法诱导帕金森病模型，随机分为模型组和药物治疗组，使用六味地黄汤、金匮肾气汤、芪脊舒僵汤进行药物反证，同时设立正常组作为对照。从模拟病因、模拟症状、客观指标、药物反佐四个方面对PD模型中医属性进行评价。,结果,2,与正常组比较，模型组PD小鼠，行为学、舌象、环磷腺苷（cAMP）/环磷鸟苷（cGMP）比值、血栓素A（TXA,2,）/前列环素（PGI,2,）比值和酪氨酸羟化酶含量均存在明显差异（,P,<,0.05）；予以中药干预后，与模型组比较，六味地黄汤组小鼠体温有所改善（,P,<,0.05），但行为学、舌象和客观指标均无明显差异（,P,>,0.05）；金匮肾气汤组和芪脊舒僵汤组小鼠僵直症状、运动能力明显恢复，体温以及舌象、cAMP/cGMP和酪氨酸羟化酶均有改善（,P,<,0.05），并且芪脊舒僵汤组治疗效果明显优于金匮肾气汤组（,P,<,0.05）。除此之外，与模型组比较，只有芪脊舒僵汤组与血瘀密切相关的指标TXA,2,/PGI,2,比值具有明显差异（,P,<,0.05）。,结论,2,采用MPTP慢性腹腔注射的方法可以成功建立病证结合PD小鼠模型，其中医证候属性为阳虚血瘀证。

Abstract

Objective： To establish combination of disease withsyndrome mous emodel with Parkinson’s disease （PD）， and to explore its attributes of traditional Chinese medicine（TCM） syndromes.,Methods,2,The models of Parkinson’s disease induced by chronic intraperitoneal injection of 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine（MPTP） were established and randomly divided into model group and drug treatment group. Liuwei Dihuang Decoction， Jinkui Shenqi Decoction， and Qiji Shujiang Decoction were used respectively for drug counterevidence. At the same time， normal group was set as control. The TCM attributes of PD model were evaluated from four aspects： simulated etiology， simulated symptoms， objective indicators， and drug counterevi-dence.,Results,2,Compared with the normal group， the PD mice in the model group had significant differences in behavior， tongue appearance， cyclic adenosine monophosphate （cAMP）/cyclic guanosine monophosphate （cGMP）ratio， thromboxane A （TXA,2,）/prostacyclin （PGI,2,）ratio and the content of tyrosine hydroxylase（,P,<,0.05）. After intervention with TCM， compared with the model group， the body temperature of mice in Liuwei Dihuang Decoction group was improved（,P,<,0.05）， but there was no significant difference in behavior， tongue appearance and objective indicators（,P,>,0.05）. The stiffness symptoms and exercise ability of the mice in Jinkui Shenqi Decoction group and Qiji Shujiang Decoction group were recovered significantly， and body temperature， tongue appearance， cAMP/cGMP and tyrosine hydroxylase were improved （,P,<,0.05）， and the therapeutic effect of Qiji Shujiang Decoction group was significantly better than that of Jinkui Shenqi Decoction group（,P,<,0.05）. In addition， compared with the model group， the ratio of TXA,2,/PGI,2,， an index closely related to blood stasis， was significantly different only in the Qiji Shujiang Decoction group （,P,<,0.05）.,Conclusion,2,Chronic intraperitoneal injection of MPTP can successfully establish combination of disease and syndrome mouse model with Parkinson’s disease， whose TCM syndrome attribute is Yang deficiency and blood stasis syndrome.

关键词

帕金森病病证结合动物模型

Keywords

references

KONNO T， DEUTSCHLÄNDER A， HECKMAN M G， et al. Comparison of clinical features among Parkinson’s disease subtypes： A large retrospective study in a single center［J］. J Neurol Sci， 2018， 386： 39-45.

MORETTI R， MILNER V， Caruso P， et al. Frontal Tasks and Behavior in Rigid or Tremor-Dominant Parkinson Disease［J］. Am J Alzheimers Dis Other Demen， 2017， 32（5）： 300-306.

苏巧珍，雒晓东. 当归四逆加吴茱萸生姜汤治疗僵直少动型帕金森病31例［J］.新中医，2007， 39（5）： 60-61.

梁艳. 芸芍帕安合剂治疗帕金森病的临床疗效及其自噬机制研究［D］. 南京：南京中医药大学，2019.

贾波，李冀. 方剂学［M］. 北京：中国中医药出版社，2014：102-109.

DHINGRA D， GOSWAMI S， GAHALAIN N. Protective effect of hesperetin against haloperidol-induced orofacial dyskinesia and catalepsy in rats［J］. Nutr Neurosci， 2018， 21（9）： 667-675.

潘志强，卢文丽，方肇勤. 小鼠舌象的显微拍摄［J］. 中国中医基础医学杂志，2007， 13（3）： 189-191.

冉秋，何建成. 帕金森病大鼠显微舌象研究［J］. 中医药信息，2009， 26（6）： 13-14.

徐鹏恒，何建成，王利，等. 复方地黄汤联合美多巴治疗帕金森病的临床疗效观察［J］. 中华中医药杂志，2019， 34（2）： 838-840.

徐栋，黄世敬，袁永娥. 中医药治疗帕金森病临床研究进展［J］. 河北中医，2019， 41（11）： 1743-1747.

GIBB W R， LEES A J. A comparison of clinical and pathological features of young- and old-onset Parkinson’s disease［J］. Neurology， 1988， 38（9）： 1402-1406.

HARIZ G M， FORSGREN L. Activities of daily living and quality of life in persons with newly diagnosed Parkinson’s disease according to subtype of disease， and in comparison to healthy controls［J］. Acta Neurol Scand， 2011， 123（1）： 20-27.

罗云坚，张英哲. 常见病中西医结合诊疗常规［M］. 广州：广东科学技术出版，2003： 175-176．

何竹青，王滢迪，王利，等. 中医学对僵直少动型帕金森病的认识及治疗［J/OL］.中华中医药学刊，2022［2021-12-16］. http：//kns.cnki.net/kcms/detail/21.1546.R.20210409.1710.033.htmlhttp://kns.cnki.net/kcms/detail/21.1546.R.20210409.1710.033.html.

吕爱平. 病证结合动物模型研究：从理论创新到技术挑战［J］. 中国中西医结合杂志，2013， 33（1）： 6-7.

郑小伟. 论中医动物模型的造型依据与研制方法［J］. 浙江中医学院学报，2004， 28（6）： 1-4.

陈生弟. 帕金森病［M］. 北京：人民卫生出版社，2006： 119-120．

滕培颍，赵瑞芝，徐福平，等. 附子半夏汤对阳虚模型大鼠不同脏器能量代谢的影响［J］. 新中医，2017， 74（2）： 4-6．

张彦卿. 基于RGB和Lab模式下慢性胃炎Hp+舌色、苔色特点的研究［D］. 广州：广州中医药大学，2011.

张译心，王鑫，刘博，等. 2型糖尿病气阴两虚病证结合动物模型的制备及评价指标的建立［J］. 中国实验动物学报，2021， 29（2）： 219-229.

冉秋，何建成. 一体化病证结合帕金森病大鼠模型中医证候属性研究［J］. 中国实验动物学报，2011，19（6）：465-471.

张翠珍，王天芳. cAMP、cGMP拮抗性代谢调节与中医证候关系研究进展［J］. 北京中医药大学学报，1999（6）： 51-53.

SANTOS M T，COSIN J，SANCHEZ E，et al. Residual platelet thromboxane A2 and prothrombotic effects of erythrocytes are important determinants of aspirin resistance in patients with vascular disease［J］. J Thromb Haemost，2008， 6（4）： 615-621.

张兰凤，王阶. 血瘀证的细胞学和分子学研究进展［J］. 中国中医基础医学杂志，2003， 9（1）： 71-72，76.

王炜为，高晗，李文涛. 补肾止颤方对6-OHDA诱导的帕金森病模型大鼠神经保护作用的研究［J］. 世界科学技术-中医药现代化，2020， 22（9）： 3270-3276.

Views

512

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Clinical observation on “Modified Wuhu Zhuifeng Powder” in the treatment of Parkinson's disease complicated with mild cognitive impairment of type of kidney deficiency and blood stasis

Related Author

No data

Related Institution

Guangxi University of Traditional Chinese Medicine

Department of Geriatrics, First Affiliated Hospital of Guangxi University of Traditional Chinese Medical

⁰