Effects of Jianpi Bushen Formula on intestinal flora in postoperative patients with colorectal cancer

TAN Yabin; ZHU Mingyang; WANG Yitong; JIANG Lujian; ZUO Minghuan; FENG Baoyue

doi:10.16306/j.1008-861x.2021.06.004

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Effects of Jianpi Bushen Formula on intestinal flora in postoperative patients with colorectal cancer

Clinical Research | 更新时间：2022-09-09

- Effects of Jianpi Bushen Formula on intestinal flora in postoperative patients with colorectal cancer
- Academic Journal of Shanghai University of Traditional Chinese Medicine Vol. 35, Issue 6, Pages: 22-30(2021)
- 作者机构：
  
  1.北京中医药大学（北京　100029）
  2.北京中医药大学东方医院肿瘤科（北京　100078）
  3.宁海县中医医院医疗健康集团（浙江　宁波　315600）
- 作者简介：
- 基金信息：
- DOI：10.16306/j.1008-861x.2021.06.004
  CLC：
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TAN Yabin, ZHU Mingyang, WANG Yitong, et al. Effects of Jianpi Bushen Formula on intestinal flora in postoperative patients with colorectal cancer. [J]. Academic Journal of Shanghai University of Traditional Chinese Medicine 35(6):22-30(2021)
DOI：

TAN Yabin, ZHU Mingyang, WANG Yitong, et al. Effects of Jianpi Bushen Formula on intestinal flora in postoperative patients with colorectal cancer. [J]. Academic Journal of Shanghai University of Traditional Chinese Medicine 35(6):22-30(2021) DOI： 10.16306/j.1008-861x.2021.06.004.

摘要

目的：,2,探讨健脾补肾方对大肠癌（CRC）术后脾肾阳虚证患者肠道菌群的影响。,方法：,2,纳入40例CRC术后脾肾阳虚证患者和10例健康志愿者，患者入组后给予健脾补肾方口服治疗，周期为14 d。健脾补肾方干预前后，评价并记录所有患者的相关临床症状评分，比较患者症状总积分的变化；采集健脾补肾方干预前后患者及健康志愿者的粪便样本，运用16SrDNA测序技术检测各样本中的肠道菌群，分析患者与健康志愿者的肠道菌群结构差异，比较健脾补肾方干预前后患者肠道菌群结构的变化。,结果：,2,①健脾补肾方干预后，CRC术后脾肾阳虚证患者的症状总积分较干预前显著降低（,P,<,0.05）。②受试患者与健康志愿者的肠道菌群结构在各分类水平上均存在差异，健脾补肾方干预前后患者的肠道菌群结构在各分类水平上均发生了改变。,结论：,2,CRC术后脾肾阳虚证患者的肠道菌群结构与健康人存在差异，健脾补肾方干预可改善患者的相关症状及肠道菌群结构，健脾补肾方改善CRC术后脾肾阳虚证患者相关症状的作用机制可能与改善患者的肠道微生态环境有关。

Abstract

Objective:,2,To investigate the effects of Jianpi Bushen Formula on intestinal flora in postoperative patients of colorectal cancer(CRC) with spleen-kidney yang deficiency syndrome.,Methods:,2,Forty postoperative patients of CRC with spleen-kidney yang deficiency syndrome and 10 healthy volunteers were included.After enrollment, the patients were orally treated with Jianpi Bushen Formula with a course of 14 days.Before and after intervention of Jianpi Bushen Formula, the relevant clinical symptom scores of all patients were evaluated and recorded, and the total symptom scores of the patients were compared.The feces specimens of the healthy volunteers and the patients before and after drug treatment were collected, the intestinal flora in all samples were detected by 16SrDNA sequencing technology, the differences of intestinal flora structure between the patients and the healthy volunteers were analyzed, and the intestinal flora structure of the patients before and after intervention of Jianpi Bushen Formula were compared.,Results:,2,①After intervention of Jianpi Bushen Formula, the total symptom score of CRC postoperative patients with spleen-kidney yang deficiency syndrome were significantly decreased compared with that before intervention(,P,<,0.05) .②There were differences in the intestinal flora structure at all classification levels between the patients and the healthy volunteers.Before and after intervention of Jianpi Bushen Formula, the intestinal flora structure of the patients changed at all classification levels.,Conclusion:,2,There are differences in the intestinal flora structure between the healthy people and the CRC postoperative patients with spleen-kidney yang deficiency syndrome.The intervention of Jianpi Bushen Formula can improve the relevant clinical symptoms and the intestinal flora structure of the patients.The mechanism of Jianpi Bushen Formula improving the relevant symptoms of CRC postoperative patients with spleen-kidney yang deficiency syndrome may be related to improving the intestinal microecological environment.

关键词

大肠癌术后脾肾阳虚证健脾补肾方肠道菌群

Keywords

colorectal cancerpostoperationspleen-kidney yang deficiency syndromeJianpi Bushen Formulaintestinal flora

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