Objective:,2,To study the effects of electroacupuncture combined with vibration training on nuclear transcription factor inhibitory protein(IκB) /nuclear transcription factor κB(NF-κB) signaling pathway and motor function in hypoxic-ischemic encephalopathy(HIE) rats.,Methods:,2,Seven days old SD rats were randomly divided into the sham operation group, model group, electroacupuncture group, vibration group and combination group, 20 rats in each group.Except the sham operation group, HIE model was established by modified Rice method in the other groups.Twenty-four hours after modeling, the electroacupuncture group was treated with electroacupuncture at Baihui, Renzhong and Dazhui acupoints, 15 minutes each time; the vibration group was treated with vibration training, 15 minutes each time; and the combination group was treated with electroacupuncture and vibration training successively.The treatments were performed once a day for 2 weeks.After the last intervention, the neurobehavior of rats was evaluated by Bederson score, and the cross-sectional area of gastrocnemius muscle of left hindlimb was measured by ultrasonic diagnostic instrument.The morphological changes of brain tissue were observed under light microscope after HE staining.The mRNA expressions of ,IκB,NF-κB p65,Bcl-2, and ,Bax, in gastrocnemius muscle were detected by PCR.The protein expressions of IκB, NF-κB p65, Bcl-2 and Bax in gastrocnemius muscle were detected by Western blot.,Results:,2,After treatment for 2 weeks, ①The Bederson score of rats in the electroacupuncture group, vibration group and combination group was significantly lower than that in the model group(,P,<,0.05,P,<,0.01), and the score of the combination group was significantly lower than that of the vibration group and the electroacupuncture group(,P,<,0.05) .②Compared with the model group, the cross-sectional area of left gastrocnemius muscle in the electroacupuncture group, vibration group and combination group was increased significantly(,P,<,0.05,P,<,0.01) .The cross-sectional area of the combination group was significantly larger than that of the electroacupuncture group and the vibration group(,P,<,0.05,P,<,0.01) .③HE staining showed that the arrangement of nerve cells in the damaged brain tissue of the model group was disordered, the intercellular space was enlarged, the structure was loose, and the vacuole-like changes, obvious cell body swelling and nuclear atrophy were observed.The pathological injury of brain tissue in the electroacupuncture group, vibration group and combination group was alleviated, and the improvement in the combination group was more significant, and the necrotic cells of brain tissue and inflammatory infiltration in ischemic area were significantly reduced.④Compared with the model group, the mRNA and protein expression levels of IκB, NF-κB p65 and Bax in gastrocnemius muscle of the electroacupuncture group, vibration group and combination group were significantly decreased, and the mRNA and protein expression levels of Bcl-2 were significantly increased(,P,<,0.05,P,<,0.01) .Compared with the electroacupuncture group and vibration group, the mRNA and protein expressions of IκB, NF-κB p65 and Bax were down-regulated in the combination group, and the mRNA and protein expressions of Bcl-2 were up-regulated(,P,<,0.05,P,<,0.01) .,Conclusion:,2,Electroacupuncture combined with vibration training can significantly improve neurobehavior and reduce brain tissue injury in HIE model rats, and the effect is better than electroacupuncture or vibration training alone.Its mechanism may be related to suppressing IκB/NF-κB signaling pathway, inhibiting cell apoptosis of skeletal muscle and improving motor function.